BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,791,298	T→C	T95T (ACA→ACG)	btuD ←	ATP‑binding component of vitamin B12 transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,791,298	0	T	C	100.0%	643.1 / NA	204	T95T (ACA→ACG)	btuD	ATP‑binding component of vitamin B12 transport system
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (107/97); total (107/97)

Only 100 of 209 total aligned reads displayed.

TTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCA  >  NC_000913/1791209‑1791387

 tGGTCCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCa                                                                                       <  1:6097895/93‑1 (MQ=255)

   gTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAgg                                                                                     <  1:4887666/93‑1 (MQ=255)

     gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTa                                                                                   <  1:2061393/93‑1 (MQ=255)

     gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTa                                                                                   <  2:4359920/93‑1 (MQ=255)

      cTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAg                                                                                  <  1:740428/93‑1 (MQ=255)

       tACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGt                                                                                 <  1:124608/93‑1 (MQ=255)

       tACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGt                                                                                 >  2:7082488/1‑93 (MQ=255)

        aCGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTg                                                                                >  2:6526142/1‑93 (MQ=255)

        aCGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTg                                                                                >  2:1444544/1‑93 (MQ=255)

         cGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGc                                                                               <  2:2792345/93‑1 (MQ=255)

           tCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             <  2:7698133/93‑1 (MQ=255)

           tCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             <  2:3928706/93‑1 (MQ=255)

              gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAc                                                                          >  2:6469005/1‑93 (MQ=255)

               aGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAcc                                                                         <  1:483585/93‑1 (MQ=255)

               aGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAcc                                                                         >  2:3982308/1‑93 (MQ=255)

                gTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCg                                                                        >  1:3622874/1‑93 (MQ=255)

                gTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCg                                                                        >  2:6514056/1‑93 (MQ=255)

                   tGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCATTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCg                                                                     <  2:7009605/93‑1 (MQ=255)

                    gTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGt                                                                    <  2:4702714/93‑1 (MQ=255)

                     tcatcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGtt                                                                   >  1:7152514/1‑93 (MQ=255)

                      catcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTg                                                                  <  1:3429771/93‑1 (MQ=255)

                       atcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGc                                                                 >  2:4040189/1‑93 (MQ=255)

                       atcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGc                                                                 >  1:6841340/1‑93 (MQ=255)

                        tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                >  1:102083/1‑93 (MQ=255)

                        tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                >  2:7370437/1‑93 (MQ=255)

                        tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                >  1:183203/1‑93 (MQ=255)

                         caAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCaa                                                               <  2:1659686/93‑1 (MQ=255)

                          aaGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCaaa                                                              <  1:1519983/93‑1 (MQ=255)

                          aaGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCaaa                                                              <  2:6861617/93‑1 (MQ=255)

                           agagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAc                                                             <  2:427149/93‑1 (MQ=255)

                           agagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAc                                                             <  2:5390287/93‑1 (MQ=255)

                           agagCCAGCGCCCCTGCGACATCATTAAGTAGTTCGGTACGCGTTGTATCGTGCTGATGCAGGGGCAGGTAGTGCCAGTCCGGCGTTGCAAAc                                                             <  2:5772362/93‑1 (MQ=255)

                             agCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcgg                                                           <  1:2683581/93‑1 (MQ=255)

                               ccAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                         <  1:3127162/93‑1 (MQ=255)

                               ccAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                         >  1:5823084/1‑93 (MQ=255)

                                 aGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACggcggc                                                       >  1:6471421/1‑93 (MQ=255)

                                  gcgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGgcggcg                                                      >  1:3573643/1‑93 (MQ=255)

                                  gcgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGgcggcg                                                      >  2:3076952/1‑93 (MQ=255)

                                      cccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctg                                                  <  1:3739130/93‑1 (MQ=255)

                                      cccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctg                                                  >  1:219454/1‑93 (MQ=255)

                                       ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 >  2:3341163/1‑93 (MQ=255)

                                        cTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGCTGCAAACGGCGGCGTctgct                                                >  2:1862582/1‑93 (MQ=255)

                                         tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               >  2:1682632/1‑93 (MQ=255)

                                         cgCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               <  1:2530541/92‑1 (MQ=255)

                                          gCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgt                                              <  2:6453614/93‑1 (MQ=255)

                                          gCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgt                                              >  2:4261669/1‑93 (MQ=255)

                                           cGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgtt                                             <  2:2504871/93‑1 (MQ=255)

                                            gACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                            >  2:6339093/1‑93 (MQ=255)

                                            gACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                            >  2:197970/1‑93 (MQ=255)

                                             aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           >  2:4419203/1‑93 (MQ=255)

                                             aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  2:3239739/93‑1 (MQ=255)

                                             aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  2:1437540/93‑1 (MQ=255)

                                               atcatTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGa                                         >  1:7424092/1‑93 (MQ=255)

                                                  atTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAg                                      >  2:3465696/1‑93 (MQ=255)

                                                   ttCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGa                                     >  2:2735085/1‑93 (MQ=255)

                                                    tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAt                                    >  2:5585175/1‑93 (MQ=255)

                                                    tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAt                                    <  2:341118/93‑1 (MQ=255)

                                                     cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   <  1:5302729/93‑1 (MQ=255)

                                                     cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   <  1:5949777/93‑1 (MQ=255)

                                                     cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   <  1:6288008/93‑1 (MQ=255)

                                                     cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   <  2:6111852/93‑1 (MQ=255)

                                                     cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATa                                   <  1:5614745/93‑1 (MQ=255)

                                                      agtcgtTCGGTACGCGTTTTATCGTGCTGATGCCGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAg                                  <  2:2952650/93‑1 (MQ=255)

                                                      agtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAg                                  <  1:4126365/93‑1 (MQ=255)

                                                      agtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAg                                  <  1:526099/93‑1 (MQ=255)

                                                       gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 >  2:1419425/1‑93 (MQ=255)

                                                       gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 <  2:6421800/93‑1 (MQ=255)

                                                       gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 >  2:2105959/1‑93 (MQ=255)

                                                        tagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAggg                                >  2:6286600/1‑92 (MQ=255)

                                                        tagtTCGGTACGCGTTTTAGCGAGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  2:2300307/93‑1 (MQ=255)

                                                        gagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  2:6949086/92‑1 (MQ=255)

                                                          gtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcgg                              >  2:2065783/1‑92 (MQ=255)

                                                          gtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcgc                              <  1:2846235/93‑1 (MQ=255)

                                                            tCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGa                            >  2:2758623/1‑93 (MQ=255)

                                                            tCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGa                            <  1:2402478/93‑1 (MQ=255)

                                                               gcaCGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTGTGCTGTTGTGGAAGATAGGCGCGATGc                         >  2:1105286/3‑93 (MQ=255)

                                                               gTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGc                         >  1:4210240/1‑93 (MQ=255)

                                                               gTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGc                         <  1:633115/93‑1 (MQ=255)

                                                                 aCGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAg                       <  2:3706562/93‑1 (MQ=255)

                                                                 aCGCGTTTCATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAg                       <  2:6796664/93‑1 (MQ=255)

                                                                     gTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGcgcg                   >  1:703198/1‑93 (MQ=255)

                                                                      ttttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGTCGGCGTCTCCTGTTGTGAAAGATAGGCGCGATGCCGCGCGa                  >  2:5898188/1‑93 (MQ=255)

                                                                      ttttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGa                  >  2:5202776/1‑93 (MQ=255)

                                                                        ttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGt                <  2:1429537/93‑1 (MQ=255)

                                                                        ttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGt                >  1:2275692/1‑93 (MQ=255)

                                                                         tATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtt               <  2:4834836/93‑1 (MQ=255)

                                                                         tATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtt               <  1:435321/93‑1 (MQ=255)

                                                                           tCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtttt             >  2:3210440/1‑93 (MQ=255)

                                                                           tCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtttt             >  1:2328310/1‑93 (MQ=255)

                                                                             gTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgt           <  2:7217903/93‑1 (MQ=255)

                                                                              tGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGACAGATAGGCGCGATGCAGCGCGAGTTttgtt          >  1:55268/1‑93 (MQ=255)

                                                                              tGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgtt          >  2:1118274/1‑93 (MQ=255)

                                                                              tGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgtt          <  1:5726943/93‑1 (MQ=255)

                                                                                cTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc        >  2:4119974/1‑93 (MQ=255)

                                                                                cTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc        <  1:5753922/93‑1 (MQ=255)

                                                                                  gATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCgg      >  1:6697811/1‑93 (MQ=255)

                                                                                  gATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCgg      >  2:1010257/1‑93 (MQ=255)

                                                                                  gATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCgg      >  1:6171630/1‑93 (MQ=255)

                                                                                      cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  >  2:5989687/1‑93 (MQ=255)

                                                                                      cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  <  1:4934158/93‑1 (MQ=255)

TTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCA  >  NC_000913/1791209‑1791387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`