BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	195,014	A→G	I38V (ATT→GTT)	yaeS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	195,014	0	A	G	99.6%	855.0 / NA	275	I38V (ATT→GTT)	yaeS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (155/119); total (155/120)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTG  >  NC_000913/194924‑195103

 cACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTc                                                                                        <  2:6245742/93‑1 (MQ=255)

 cACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTc                                                                                        <  2:1143715/93‑1 (MQ=255)

  aCTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCg                                                                                       <  1:7836863/93‑1 (MQ=255)

  aCTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCg                                                                                       <  1:3163525/93‑1 (MQ=255)

   cTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGt                                                                                      >  1:2651371/1‑93 (MQ=255)

    ttAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTg                                                                                     >  1:2403667/1‑93 (MQ=255)

    ttAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAACCAAGGGAAGGTTCGTg                                                                                     <  2:5218814/93‑1 (MQ=255)

        cGAAAAATTGCCACCGCGTGGCTTCCGTCAGGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAATGTTCGTGCCtt                                                                                 <  1:2483653/93‑1 (MQ=255)

         gAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCttt                                                                                >  2:5588435/1‑93 (MQ=255)

         gAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCttt                                                                                <  1:1684246/93‑1 (MQ=255)

         gAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCttt                                                                                <  2:6315233/93‑1 (MQ=255)

          aaaaaTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTg                                                                               <  1:5875610/93‑1 (MQ=255)

           aaaaTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTgg                                                                              <  2:436918/93‑1 (MQ=255)

              aTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCTGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCa                                                                           <  2:4682037/93‑1 (MQ=255)

              aTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCa                                                                           >  1:4105792/1‑93 (MQ=255)

                  ccAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATaaa                                                                       >  1:1651216/1‑93 (MQ=255)

                  ccAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATaaa                                                                       >  2:5034827/1‑93 (MQ=255)

                   cAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAg                                                                      >  1:2480228/1‑93 (MQ=255)

                   cAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAg                                                                      >  2:3112204/1‑93 (MQ=255)

                    aGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGc                                                                     <  1:7012657/93‑1 (MQ=255)

                     gcgcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGcc                                                                    <  1:769920/93‑1 (MQ=255)

                      cgcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCg                                                                   <  1:4350390/93‑1 (MQ=255)

                       gcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCgg                                                                  <  1:1338921/93‑1 (MQ=255)

                        cATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCggg                                                                 >  2:875100/1‑93 (MQ=255)

                        cATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCggg                                                                 >  2:6829194/1‑93 (MQ=255)

                          tGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGc                                                               <  2:3779088/93‑1 (MQ=255)

                          tGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGc                                                               >  2:1320737/1‑93 (MQ=255)

                          tGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGCTCGTGCCTTTGGGCATAAAGCCGCGGc                                                               >  1:97259/1‑93 (MQ=255)

                           ggCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCa                                                              >  1:4203880/1‑93 (MQ=255)

                           ggCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCa                                                              <  2:7893297/93‑1 (MQ=255)

                           ggCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCa                                                              >  1:1746883/1‑93 (MQ=255)

                              tGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaaa                                                           >  2:5241117/1‑93 (MQ=255)

                              tGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaaa                                                           <  2:3916157/93‑1 (MQ=255)

                               gCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAt                                                          <  2:4814331/93‑1 (MQ=255)

                               gCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAt                                                          >  1:4444745/1‑93 (MQ=255)

                                ccGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtc                                                         <  1:6004806/93‑1 (MQ=255)

                                 cGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtcc                                                        >  1:3082063/1‑93 (MQ=255)

                                  gTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCATGCCTTTGGGCATAAAGCCGGGGCAAAAtccg                                                       >  2:3393165/1‑93 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     >  2:7100981/1‑93 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     >  2:6505586/1‑93 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     >  2:925966/1‑93 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     <  1:6743250/93‑1 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     >  1:3051354/1‑93 (MQ=255)

                                     aTGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtcc                                                    >  1:6394268/1‑93 (MQ=255)

                                     aTGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtcc                                                    >  1:61219/1‑93 (MQ=255)

                                     aTGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtcc                                                    >  1:3229628/1‑93 (MQ=255)

                                     aTGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtcc                                                    <  2:7513033/93‑1 (MQ=255)

                                      tGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtccg                                                   >  1:6073896/1‑93 (MQ=255)

                                       gTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTccgc                                                  >  2:4912281/1‑93 (MQ=255)

                                         tGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTccgccg                                                <  1:3940896/93‑1 (MQ=255)

                                          gCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCgg                                               >  1:4847537/1‑93 (MQ=255)

                                             aTCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCt                                            >  1:2979986/1‑93 (MQ=255)

                                              tCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTg                                           >  2:2074291/1‑93 (MQ=255)

                                                aTTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGtc                                         <  2:5456248/93‑1 (MQ=255)

                                                    tGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtt                                     <  2:5122766/93‑1 (MQ=255)

                                                    tGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtt                                     >  2:1568866/1‑93 (MQ=255)

                                                     ggACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCttt                                    <  1:4021099/93‑1 (MQ=255)

                                                       aCGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTg                                  <  2:6667669/93‑1 (MQ=255)

                                                        cGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGc                                 <  1:1855758/93‑1 (MQ=255)

                                                        cGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGc                                 <  2:2281182/93‑1 (MQ=255)

                                                         ggCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCg                                >  2:7748918/1‑93 (MQ=255)

                                                         ggCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCg                                <  1:1276492/93‑1 (MQ=255)

                                                         ggCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCg                                <  1:7345461/93‑1 (MQ=255)

                                                           gaaTGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGc                              <  1:5657177/92‑1 (MQ=255)

                                                                gCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaaca                         >  1:2169600/1‑93 (MQ=255)

                                                                gCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaaca                         <  1:7206120/93‑1 (MQ=255)

                                                                 ccGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaacaa                        <  1:2064539/93‑1 (MQ=255)

                                                                  cGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCaacaac                       <  2:4574771/93‑1 (MQ=255)

                                                                  cGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCaacaac                       <  2:448565/93‑1 (MQ=255)

                                                                  cGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCaacaac                       >  1:5294625/1‑93 (MQ=255)

                                                                  cGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCaacaac                       <  2:3479781/93‑1 (MQ=255)

                                                                   gCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACg                      >  2:3446686/1‑93 (MQ=255)

                                                                    cTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACgg                     <  1:1560800/93‑1 (MQ=255)

                                                                    cTGGGCAAAAAAGCAAGCGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACgg                     <  2:4720049/93‑1 (MQ=255)

                                                                     tGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGt                    >  2:3009972/1‑93 (MQ=255)

                                                                     tGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGt                    >  2:6010571/1‑93 (MQ=255)

                                                                     tGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGt                    <  2:7242200/93‑1 (MQ=255)

                                                                     tGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGt                    <  2:735822/93‑1 (MQ=255)

                                                                      gggCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTa                   >  1:6241534/1‑93 (MQ=255)

                                                                      gggCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTa                   <  1:7618432/93‑1 (MQ=255)

                                                                      gggCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTa                   >  1:4883084/1‑93 (MQ=255)

                                                                         cAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTg                >  1:87187/1‑93 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa               <  1:3665219/93‑1 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa               <  1:283364/93‑1 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCCCCCGGCTGTCTCTTTTGCGGCCAACACCGGGAttta               >  2:5798397/1‑91 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTGGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCGGTCTCTTTTGCGGCCAACAACGGTATTGa               <  2:1440315/93‑1 (MQ=255)

                                                                             aaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGc            >  2:261846/1‑93 (MQ=255)

                                                                             aaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGc            >  1:5125670/1‑93 (MQ=255)

                                                                              aaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCg           >  1:5709275/1‑93 (MQ=255)

                                                                               aGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACATCGGTATCGAGGCGt          >  1:6093862/1‑93 (MQ=255)

                                                                               aGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGt          <  2:2389062/93‑1 (MQ=255)

                                                                                gCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGtt         >  1:7431985/1‑93 (MQ=255)

                                                                                  aaGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTaa       <  2:2786352/93‑1 (MQ=255)

                                                                                  aaGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTaa       >  2:3203270/1‑93 (MQ=255)

                                                                                  aaGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTaa       >  1:5313682/1‑93 (MQ=255)

                                                                                  aaGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTaa       <  2:3874931/93‑1 (MQ=255)

                                                                                  aaGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTaa       <  1:7280575/93‑1 (MQ=255)

                                                                                   aGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAAc      <  1:1029393/93‑1 (MQ=255)

                                                                                   aGGGAAGGTGCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAAc      <  1:2461453/93‑1 (MQ=255)

                                                                                       aaGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTg  >  1:3408298/1‑93 (MQ=255)

CCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTG  >  NC_000913/194924‑195103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`