BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,950,024	T→C	G202G (GGT→GGC)	yecE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,950,024	0	T	C	99.1%	668.1 / ‑4.0	214	G202G (GGT→GGC)	yecE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (99/113); total (100/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

TGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTT  >  NC_000913/1949934‑1950113

tGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAg                                                                                         >  1:7547631/1‑93 (MQ=255)

 gAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGt                                                                                        <  2:4263492/93‑1 (MQ=255)

  aaGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtg                                                                                       <  1:3114551/93‑1 (MQ=255)

   aGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGATGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtga                                                                                      <  1:2499556/93‑1 (MQ=255)

   aGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtga                                                                                      <  2:2070012/93‑1 (MQ=255)

    gCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtgat                                                                                     >  2:4503002/1‑93 (MQ=255)

    gCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtgat                                                                                     >  2:6627632/1‑93 (MQ=255)

     cTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtgatg                                                                                    >  2:2157502/1‑93 (MQ=255)

      tATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtgatga                                                                                   >  2:3448791/1‑93 (MQ=255)

      tATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGtgatga                                                                                   <  2:3244467/93‑1 (MQ=255)

        ttCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGata                                                                                 <  2:147370/93‑1 (MQ=255)

         tCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGatat                                                                                >  2:4115903/1‑93 (MQ=255)

           gcgACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGa                                                                              >  2:7712546/1‑93 (MQ=255)

            cgACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGAc                                                                             <  2:33686/93‑1 (MQ=255)

             gACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACg                                                                            >  1:6546661/1‑93 (MQ=255)

             gACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACg                                                                            <  2:4544529/93‑1 (MQ=255)

              aCGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGc                                                                           >  1:7318932/1‑93 (MQ=255)

              aCGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGc                                                                           <  1:1333382/93‑1 (MQ=255)

              aCGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGc                                                                           >  2:4999738/1‑93 (MQ=255)

                 cTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCaaa                                                                        >  1:1146741/1‑93 (MQ=255)

                  tCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCaaaa                                                                       >  2:3692971/1‑93 (MQ=255)

                   cAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAAc                                                                      <  2:7445299/93‑1 (MQ=255)

                   cAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAAc                                                                      <  1:3755834/93‑1 (MQ=255)

                   cAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAAc                                                                      >  2:6953999/1‑93 (MQ=255)

                    aaCGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAAcc                                                                     >  2:7080260/1‑93 (MQ=255)

                    aaCGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAAcc                                                                     >  1:4826308/1‑93 (MQ=255)

                     aCGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCg                                                                    >  1:6969853/1‑93 (MQ=255)

                     aCGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCg                                                                    >  1:24179/1‑93 (MQ=255)

                         aaaaaaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGaa                                                                >  2:4441427/1‑93 (MQ=255)

                           aaaaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAatt                                                              >  1:4385109/1‑93 (MQ=255)

                            aaaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAatta                                                             <  1:7494404/93‑1 (MQ=255)

                            aaaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAatta                                                             <  1:6408813/93‑1 (MQ=255)

                             aaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAattat                                                            <  2:2783605/93‑1 (MQ=255)

                             aaCCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAattat                                                            >  1:2164053/1‑93 (MQ=255)

                               ccTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTAttt                                                          >  1:3490286/1‑93 (MQ=255)

                               ccTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTAttt                                                          <  2:1832042/93‑1 (MQ=255)

                               ccTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTAttt                                                          <  1:965853/93‑1 (MQ=255)

                                cTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGTTCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTc                                                         <  2:6670978/93‑1 (MQ=255)

                                cTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTc                                                         <  2:7765048/93‑1 (MQ=255)

                                cTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTc                                                         <  2:2930181/93‑1 (MQ=255)

                                 tAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCa                                                        >  1:942914/1‑93 (MQ=255)

                                 tAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCa                                                        >  1:3212891/1‑93 (MQ=255)

                                 caaaGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCa                                                        <  1:1891653/92‑1 (MQ=255)

                                  aaaGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAg                                                       <  2:5977978/93‑1 (MQ=255)

                                    aGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGt                                                     >  2:1829325/1‑93 (MQ=255)

                                      ttCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCt                                                   >  2:4936705/1‑93 (MQ=255)

                                       tCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTg                                                  >  1:707103/1‑93 (MQ=255)

                                       tCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTg                                                  >  1:1909215/1‑93 (MQ=255)

                                       tCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTg                                                  <  1:1918164/93‑1 (MQ=255)

                                        ccGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTgg                                                 <  2:608132/93‑1 (MQ=255)

                                        ccGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTgg                                                 <  2:824730/93‑1 (MQ=255)

                                          ggTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGtt                                               <  2:4816781/93‑1 (MQ=255)

                                          ggTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGtt                                               <  1:4225619/93‑1 (MQ=255)

                                           gTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTa                                              <  2:6273703/93‑1 (MQ=255)

                                            tACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTAc                                             >  1:6634854/1‑93 (MQ=255)

                                            tACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTAc                                             <  2:3732576/93‑1 (MQ=255)

                                             aCATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACa                                            <  2:2436112/93‑1 (MQ=255)

                                              cATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaa                                           >  1:3032907/1‑93 (MQ=255)

                                               aTGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTAATGATGTGACGCAAAACCGGGAATTATTTAAGGACTGTTTACaca                                          >  1:2648330/1‑91 (MQ=255)

                                                tGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaaaa                                         >  1:6265926/1‑93 (MQ=255)

                                                tGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaaaa                                         >  2:6661009/1‑93 (MQ=255)

                                                 gCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACaaaaa                                        <  2:7225135/93‑1 (MQ=255)

                                                  cTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAAt                                       >  2:4360613/1‑93 (MQ=255)

                                                    gTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTa                                     <  2:5652542/93‑1 (MQ=255)

                                                        tGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAgcgc                                 >  1:1032159/1‑93 (MQ=255)

                                                        tGACGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAgcgc                                 >  1:148551/1‑93 (MQ=255)

                                                         gACGGCGACAAATCTGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCa                                <  1:1201741/93‑1 (MQ=255)

                                                          aCGGCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAg                               <  1:2133226/93‑1 (MQ=255)

                                                             gCGACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTgg                            >  2:6404708/1‑93 (MQ=255)

                                                               gACAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCa                          <  2:7614312/93‑1 (MQ=255)

                                                               gACAAATCCGCTGATCCGCTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATCTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCa                          <  1:4505587/93‑1 (MQ=255)

                                                                aCAAATCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCAt                         <  1:1660433/93‑1 (MQ=255)

                                                                  aaaTCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCa                       <  2:2292018/93‑1 (MQ=255)

                                                                    aTCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGa                     <  1:2503886/93‑1 (MQ=255)

                                                                    aTCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGa                     >  2:1994198/1‑93 (MQ=255)

                                                                     tCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGAc                    >  2:5522129/1‑93 (MQ=255)

                                                                     tCCGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGAc                    <  2:1030180/93‑1 (MQ=255)

                                                                      ccGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGAcc                   >  1:3680299/1‑93 (MQ=255)

                                                                       cGCTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCa                  >  1:3539656/1‑93 (MQ=255)

                                                                         cTGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACt                >  1:5401221/1‑93 (MQ=255)

                                                                          tGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTa               <  1:2932460/93‑1 (MQ=255)

                                                                          tGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTa               <  2:2515338/93‑1 (MQ=255)

                                                                          tGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTa               <  1:1581052/93‑1 (MQ=255)

                                                                          tGATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTa               <  2:2666404/93‑1 (MQ=255)

                                                                          tGATCCGTTGTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTa               <  1:1873712/93‑1 (MQ=255)

                                                                           gATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTAc              >  2:706221/1‑93 (MQ=255)

                                                                           gATCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTAc              <  1:5719366/93‑1 (MQ=255)

                                                                             tCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGc            >  1:5095812/1‑93 (MQ=255)

                                                                             tCCGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGc            <  1:1252844/93‑1 (MQ=255)

                                                                              ccGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGcc           >  1:4685812/1‑93 (MQ=255)

                                                                               cGTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCt          <  2:317881/93‑1 (MQ=255)

                                                                                gTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCtt         >  1:3975667/1‑93 (MQ=255)

                                                                                gTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCtt         >  1:3300147/1‑93 (MQ=255)

                                                                                gTTTTATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCtt         >  1:761572/1‑93 (MQ=255)

                                                                                 ttttATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTa        >  2:5137403/1‑93 (MQ=255)

                                                                                 ttttATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTa        <  2:7285926/93‑1 (MQ=255)

                                                                                 ttttATCGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTa        <  2:1088951/93‑1 (MQ=255)

                                                                                       cGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCtttt  <  1:1841222/93‑1 (MQ=255)

                                                                                       cGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCtttt  <  2:2144791/93‑1 (MQ=255)

                                                                                       cGGCAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTAAGCCTTATCtttt  <  1:4831136/93‑1 (MQ=255)

TGAAGCTATTCGCGACGCTCAACGAAAAAAACCTAAAGTTCCGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTT  >  NC_000913/1949934‑1950113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`