BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,958,588	T→C	M168T (ATG→ACG)	argS →	arginine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,958,588	0	T	C	99.1%	658.4 / ‑3.0	214	M168T (ATG→ACG)	argS	arginine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (105/107); total (106/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

Only 100 of 222 total aligned reads displayed.

TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAA  >  NC_000913/1958498‑1958675

ttGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGc                                                                                       <  2:3434263/93‑1 (MQ=255)

   gTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGa                                                                                    <  2:6297856/93‑1 (MQ=255)

    tGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAt                                                                                   <  1:7086970/93‑1 (MQ=255)

     gACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAtt                                                                                  <  2:4787864/93‑1 (MQ=255)

        gcagcaGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCa                                                                               <  2:2411654/93‑1 (MQ=255)

        gcagcaGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCa                                                                               <  2:1992227/93‑1 (MQ=255)

         cagcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAt                                                                              <  2:1134118/93‑1 (MQ=255)

          agcatTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATg                                                                             <  2:7138953/93‑1 (MQ=255)

           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                            >  2:5930896/1‑93 (MQ=255)

           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                            >  1:2341876/1‑93 (MQ=255)

           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                            <  2:727214/93‑1 (MQ=255)

             agTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCt                                                                          >  2:363403/1‑93 (MQ=255)

             agTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCt                                                                          >  1:1297551/1‑93 (MQ=255)

              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                         >  2:6857673/1‑93 (MQ=255)

              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                         >  2:7738329/1‑93 (MQ=255)

                gCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGa                                                                       >  1:3871774/1‑93 (MQ=255)

                  gTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaa                                                                     >  2:3833398/1‑93 (MQ=255)

                   tACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaaa                                                                    <  2:4868637/93‑1 (MQ=255)

                    aCTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAag                                                                   <  1:2775542/93‑1 (MQ=255)

                      tctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagca                                                                 >  2:134018/1‑93 (MQ=255)

                      tctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagca                                                                 <  1:1541354/93‑1 (MQ=255)

                       ctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcag                                                                <  2:733001/93‑1 (MQ=255)

                          gAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagcag                                                             >  2:4808615/1‑93 (MQ=255)

                            gTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                           >  1:1895396/1‑93 (MQ=255)

                            gTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                           >  1:5625532/1‑93 (MQ=255)

                            gTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                           <  2:5500282/93‑1 (MQ=255)

                             ttCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGcagcagcag                                                          <  2:3895939/93‑1 (MQ=255)

                             ttCCTCGCTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGcagcagcag                                                          <  2:2205481/93‑1 (MQ=255)

                               ccTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGa                                                        <  2:2609510/93‑1 (MQ=255)

                               ccTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGa                                                        <  1:3812397/93‑1 (MQ=255)

                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                       >  1:1126661/1‑93 (MQ=255)

                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                       >  2:7191063/1‑93 (MQ=255)

                                 tCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaa                                                      >  1:1469964/1‑93 (MQ=255)

                                   ggTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAc                                                    >  1:7220848/1‑93 (MQ=255)

                                      cacaAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGcc                                                 <  2:4652102/93‑1 (MQ=255)

                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                               <  2:4065396/93‑1 (MQ=255)

                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                               <  1:3703894/93‑1 (MQ=255)

                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                               >  1:7608414/1‑93 (MQ=255)

                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGt                                              <  1:6859613/93‑1 (MQ=255)

                                          aaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTg                                             >  1:3542422/1‑93 (MQ=255)

                                          aaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTg                                             >  1:7546499/1‑93 (MQ=255)

                                            gTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaa                                           <  2:1298580/93‑1 (MQ=255)

                                            gTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaa                                           >  1:3279150/1‑93 (MQ=255)

                                             tGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaaa                                          >  2:3140375/1‑93 (MQ=255)

                                             tGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaaa                                          >  1:677536/1‑93 (MQ=255)

                                              gATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAt                                         <  2:6284308/93‑1 (MQ=255)

                                               gttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATg                                        >  1:3362981/2‑93 (MQ=255)

                                               aTTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATg                                        <  2:2460603/93‑1 (MQ=255)

                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       >  1:3218043/1‑93 (MQ=255)

                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       <  2:4440726/93‑1 (MQ=255)

                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       >  2:4559980/1‑93 (MQ=255)

                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       <  2:1532398/93‑1 (MQ=255)

                                                 tCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGa                                      <  1:5466027/93‑1 (MQ=255)

                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    >  2:2063176/1‑93 (MQ=255)

                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    <  1:757471/93‑1 (MQ=255)

                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    <  1:5285374/93‑1 (MQ=255)

                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    <  1:6064197/93‑1 (MQ=255)

                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                   <  1:3018561/93‑1 (MQ=255)

                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                   <  2:1379959/93‑1 (MQ=255)

                                                     gcAAACCACGTCGGCGACTGGGGCCCTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctg                                  >  2:7588573/1‑93 (MQ=255)

                                                     gcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctg                                  >  1:6505671/1‑93 (MQ=255)

                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                >  2:7922015/1‑93 (MQ=255)

                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                >  2:658509/1‑93 (MQ=255)

                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                            <  2:4604906/93‑1 (MQ=255)

                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                           <  2:5395305/93‑1 (MQ=255)

                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                           <  1:4467937/93‑1 (MQ=255)

                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                          <  1:7263920/93‑1 (MQ=255)

                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                          <  1:20396/93‑1 (MQ=255)

                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                          <  1:4850116/93‑1 (MQ=255)

                                                              ctCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCtt                         <  1:789526/92‑1 (MQ=255)

                                                              ctCGGCGACTGGGGCACGCATTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCtt                         <  2:289550/92‑1 (MQ=255)

                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                        >  1:877859/1‑93 (MQ=255)

                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGCGAAATGGAGCTGGCTGACCTTg                        >  1:4639409/1‑93 (MQ=255)

                                                               tCGGCGACTGGAGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                        <  1:57461/93‑1 (MQ=255)

                                                                cGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGa                       >  2:5559668/1‑93 (MQ=255)

                                                                 ggCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGaa                      <  2:6451376/93‑1 (MQ=255)

                                                                   cGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAgg                    <  2:6038581/93‑1 (MQ=255)

                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                   <  1:815736/93‑1 (MQ=255)

                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                   >  1:7057627/1‑93 (MQ=255)

                                                                     aCTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGtt                  >  2:521029/1‑93 (MQ=255)

                                                                      cTGGGGCACTCAGTTCGGTACGGTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGttt                 >  1:7526506/1‑93 (MQ=255)

                                                                      cTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGttt                 >  2:3793238/1‑93 (MQ=255)

                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAGGCAGCAGCAGGAAAACGCCGGTGCAATGGAGCTGGCTGACCTTGAAGGTTTc                >  1:1269390/1‑93 (MQ=255)

                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                <  2:2754326/93‑1 (MQ=255)

                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                >  2:7644846/1‑93 (MQ=255)

                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCt               >  1:4072551/1‑93 (MQ=255)

                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCt               <  1:106554/93‑1 (MQ=255)

                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGGGAAATGGAGCGGGCTGGCCTTGAAGTGTTCt               >  1:2534360/1‑93 (MQ=255)

                                                                             aCTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcgc          >  2:2368718/1‑93 (MQ=255)

                                                                              cTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcgcg         >  1:6411948/1‑93 (MQ=255)

                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  1:4781008/1‑93 (MQ=255)

                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  2:5406246/1‑93 (MQ=255)

                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  2:3503219/1‑93 (MQ=255)

                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  1:3579478/1‑93 (MQ=255)

                                                                                 aGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATg      >  1:7385522/1‑93 (MQ=255)

                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    >  2:1742522/1‑93 (MQ=255)

                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    <  1:1448267/93‑1 (MQ=255)

                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    >  1:1277076/1‑93 (MQ=255)

                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    >  1:4240930/1‑93 (MQ=255)

                                                                                     cGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGaa  <  2:5625565/93‑1 (MQ=255)

TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAA  >  NC_000913/1958498‑1958675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`