BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,964,115	G→A	S34L (TCA→TTA)	flhB ←	FlhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,964,115	0	G	A	99.5%	768.8 / NA	214	S34L (TCA→TTA)	flhB	FlhB
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (106/107); minor base C (1/0); total (107/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTC  >  NC_000913/1964025‑1964204

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  1:3961533/93‑1 (MQ=255)

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  1:5280031/93‑1 (MQ=255)

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  2:457757/93‑1 (MQ=255)

aGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAg                                                                                         <  1:2306811/93‑1 (MQ=255)

 gCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAgg                                                                                        >  1:4885751/1‑93 (MQ=255)

 gCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAgg                                                                                        <  2:138536/93‑1 (MQ=255)

  cccAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCCACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAGATCAGCAGTAAGGt                                                                                       <  2:82212/93‑1 (MQ=255)

      gCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGt                                                                                   >  2:6917040/1‑93 (MQ=255)

        ggAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTc                                                                                 >  2:6191187/1‑93 (MQ=255)

          agagCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCAc                                                                               <  2:5747304/93‑1 (MQ=255)

           aagCATGCCCGACAATCGACGGGCCAGCGACACGCCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACg                                                                              <  2:4904522/92‑1 (MQ=255)

            agCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACgg                                                                             >  2:3784692/1‑93 (MQ=255)

            agCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACgg                                                                             <  2:12467/93‑1 (MQ=255)

            agCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACgg                                                                             <  1:1337263/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  1:2692229/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  2:1702391/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  1:594397/93‑1 (MQ=255)

               aTGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGaa                                                                          <  1:4450060/93‑1 (MQ=255)

                tGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         >  2:2910474/1‑93 (MQ=255)

                tGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         >  1:1160042/1‑93 (MQ=255)

                tGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         >  1:2455244/1‑93 (MQ=255)

                tGCCCGACAATCGACGGGCCAGAGACACACCGCCAAACCAGATAACACTAAAGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGAGAAc                                                                         >  2:1021835/1‑93 (MQ=255)

                ggCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAc                                                                         <  2:5347842/92‑1 (MQ=255)

                  cccGACAATCGACGGGCCAGCGACACGCCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAcgc                                                                       <  1:3031405/93‑1 (MQ=255)

                  cccGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCATTTCACGGGAAcgc                                                                       <  2:1577385/93‑1 (MQ=255)

                   ccGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAAcgcg                                                                      >  1:7141702/1‑93 (MQ=255)

                    cGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCgg                                                                     <  2:3353044/93‑1 (MQ=255)

                    cGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCgg                                                                     >  2:790049/1‑93 (MQ=255)

                    cGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGGAAGGTCAGTTCACGGGAACGCgg                                                                     >  2:4331207/1‑93 (MQ=255)

                      aCAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGa                                                                   >  1:776164/1‑93 (MQ=255)

                      aCAACCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGa                                                                   <  2:7139938/93‑1 (MQ=255)

                       cAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAt                                                                  <  2:4806759/93‑1 (MQ=255)

                       cAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAt                                                                  >  2:1042423/1‑93 (MQ=255)

                        aaTCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAtt                                                                 <  1:3610699/93‑1 (MQ=255)

                         aTCGACGGGCCAGCGCCACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGAttt                                                                <  2:3426284/93‑1 (MQ=255)

                          tCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTg                                                               >  1:5348053/1‑93 (MQ=255)

                          tCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTg                                                               >  2:3258752/1‑93 (MQ=255)

                               gggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCct                                                          >  1:3114235/1‑93 (MQ=255)

                               gggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCct                                                          >  2:6488982/1‑93 (MQ=255)

                                ggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctc                                                         <  1:196623/93‑1 (MQ=255)

                                ggCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctc                                                         <  2:2764519/93‑1 (MQ=255)

                                 gCCAGCGACACACCGCGAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctct                                                        <  1:1011752/93‑1 (MQ=255)

                                 gCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCctct                                                        <  1:7645992/93‑1 (MQ=255)

                                   cAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTc                                                      >  1:4571681/1‑93 (MQ=255)

                                   cAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTc                                                      <  2:3037957/93‑1 (MQ=255)

                                     gCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTccc                                                    <  1:5452927/93‑1 (MQ=255)

                                       gACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCcgc                                                  <  1:7686542/93‑1 (MQ=255)

                                       gACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCcgc                                                  >  2:6910302/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  2:7087564/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  1:7086275/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  1:3185825/1‑93 (MQ=255)

                                          acacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCt                                               >  2:4045550/1‑93 (MQ=255)

                                           cacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCtt                                              >  2:5969188/1‑93 (MQ=255)

                                           cacCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCtt                                              >  1:286415/1‑93 (MQ=255)

                                            cccGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCttt                                             <  1:1670041/92‑1 (MQ=255)

                                             ccGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCtttt                                            <  2:6127110/93‑1 (MQ=255)

                                             acGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCGCTCTTCCCGCGCtttt                                            >  2:2491085/2‑93 (MQ=255)

                                               gCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTc                                          <  2:2602055/93‑1 (MQ=255)

                                               gCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTc                                          >  1:6589423/1‑93 (MQ=255)

                                                 cAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTa                                        <  1:451054/93‑1 (MQ=255)

                                                   aaCCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGt                                      >  2:4130151/1‑93 (MQ=255)

                                                    aCCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTc                                     <  1:6057334/93‑1 (MQ=255)

                                                     ccAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCg                                    >  2:1112664/1‑93 (MQ=255)

                                                      cAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCgg                                   <  1:2968060/93‑1 (MQ=255)

                                                      cAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCgg                                   <  2:6407141/93‑1 (MQ=255)

                                                      cAGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCgg                                   >  1:7238115/1‑93 (MQ=255)

                                                       aGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCggg                                  >  1:3559214/1‑92 (MQ=255)

                                                       aGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGt                                  >  2:4082394/1‑93 (MQ=255)

                                                       aGATAACACTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGt                                  <  2:2417076/93‑1 (MQ=255)

                                                            acacTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgt                             <  1:4280503/93‑1 (MQ=255)

                                                            acacTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgt                             <  1:7699515/93‑1 (MQ=255)

                                                             cacTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgtg                            >  2:7325080/1‑93 (MQ=255)

                                                              acTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgtgt                           >  1:3253335/1‑93 (MQ=255)

                                                              acTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGgtgt                           <  2:6764778/93‑1 (MQ=255)

                                                               cTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGtgtg                          >  2:6833742/1‑93 (MQ=255)

                                                               cTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGtgtg                          <  2:637235/93‑1 (MQ=255)

                                                               cTAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGtgtg                          >  1:1457795/1‑93 (MQ=255)

                                                                tAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTgg                         <  2:5487213/93‑1 (MQ=255)

                                                                tAACGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTgg                         <  1:7456702/93‑1 (MQ=255)

                                                                 aaCGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTggg                        <  1:7303592/93‑1 (MQ=255)

                                                                  aCGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTgggg                       <  1:4684398/93‑1 (MQ=255)

                                                                   cGCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGTATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTggggg                      <  2:5321351/93‑1 (MQ=255)

                                                                    gCCCACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGc                     <  2:561991/93‑1 (MQ=255)

                                                                     cccACCAGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCt                    >  2:5252492/1‑93 (MQ=255)

                                                                      ccaccaGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCtt                   >  1:5191477/1‑93 (MQ=255)

                                                                       caccaGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTc                  >  1:4418871/1‑93 (MQ=255)

                                                                        accaGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCTGTGAGGTGTGGGGGCTTCt                 >  1:5699813/1‑93 (MQ=255)

                                                                           aGCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGtt              >  1:675445/1‑93 (MQ=255)

                                                                            gCAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGttt             >  1:4115649/1‑93 (MQ=255)

                                                                             cAAAATCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGtttt            >  2:7640005/1‑93 (MQ=255)

                                                                              aaaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTg           >  1:4955007/1‑93 (MQ=255)

                                                                               aaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgt          >  2:2385649/1‑93 (MQ=255)

                                                                               aaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgt          <  2:1911698/93‑1 (MQ=255)

                                                                               aaaTCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgt          <  1:2656649/93‑1 (MQ=255)

                                                                                  tCAGCAGTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTgtcgt       <  2:7278915/93‑1 (MQ=255)

                                                                                      cagTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGct   <  1:2375394/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:5521695/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:559653/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:5959816/93‑1 (MQ=255)

                                                                                       agTAAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGctc  <  1:2103543/93‑1 (MQ=255)

AGCCCAGCGGAGAGCATGCCCGACAATCGACGGGCCAGCGACACACCGCCAAACCAGATAACACTAACGCCCACCAGCAAAATCAGCAGTGAGGTCAGTTCACGGGAACGCGGGATTTGCCCCTCTTCCCGCGCTTTTTCTAGTCGGTGAGGTGTGGGGGCTTCTGTTTTGTCGTCGCTC  >  NC_000913/1964025‑1964204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`