BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,975,207	T→C	intergenic (‑44/+83)	motA ← / ← flhC	MotA/FlhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,975,207	0	T	C	99.6%	781.2 / NA	252	intergenic (‑44/+83)	motA/flhC	MotA/FlhC
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (139/112); total (140/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 274 total aligned reads displayed.

CCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACA  >  NC_000913/1975117‑1975296

ccgAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCa                                                                                         >  2:4067802/1‑93 (MQ=255)

 cgAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAg                                                                                        >  2:3269361/1‑93 (MQ=255)

  gAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAgg                                                                                       >  2:4798395/1‑93 (MQ=255)

  gAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAgg                                                                                       <  2:6389184/93‑1 (MQ=255)

   aaaaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGc                                                                                      >  1:1836882/1‑93 (MQ=255)

   aaaaCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGc                                                                                      >  1:2787643/1‑93 (MQ=255)

      aCTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGtt                                                                                   >  1:4846376/1‑93 (MQ=255)

        tGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTaa                                                                                 <  1:4113911/93‑1 (MQ=255)

          tACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcg                                                                               >  2:5860404/1‑93 (MQ=255)

           aCCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcgc                                                                              <  1:1648219/93‑1 (MQ=255)

           aCCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcgc                                                                              <  1:5915008/93‑1 (MQ=255)

           aCCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcgc                                                                              <  2:3114806/93‑1 (MQ=255)

            ccGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcgcg                                                                             >  2:6069200/1‑93 (MQ=255)

            ccGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcgcg                                                                             >  1:3564512/1‑93 (MQ=255)

             cGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAAcgcgc                                                                            >  2:7152580/1‑93 (MQ=255)

                gaACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGAt                                                                         >  1:5548403/1‑93 (MQ=255)

                 aacaacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGAtt                                                                        <  2:2414074/93‑1 (MQ=255)

                 aacaacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGAtt                                                                        >  1:4593538/1‑93 (MQ=255)

                  acaacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTg                                                                       <  2:3252163/93‑1 (MQ=255)

                  acaacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTg                                                                       <  1:6875060/93‑1 (MQ=255)

                   caacCAGGTAACCTAATAAGATAAGCACGACATTATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTgg                                                                      <  1:7607409/93‑1 (MQ=255)

                    aacCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTggg                                                                     >  2:3479921/1‑93 (MQ=255)

                     acCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTgggg                                                                    >  2:5848713/1‑93 (MQ=255)

                      ccAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGc                                                                   >  2:6343709/1‑93 (MQ=255)

                      ccAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGc                                                                   <  1:7146150/93‑1 (MQ=255)

                      ccAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGc                                                                   <  1:2576319/93‑1 (MQ=255)

                        aGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCaa                                                                 >  2:3766570/1‑93 (MQ=255)

                        aGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCaa                                                                 >  1:334854/1‑93 (MQ=255)

                             aCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCaaaaaaa                                                            <  1:1374024/93‑1 (MQ=255)

                             aCCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCaaaaaaa                                                            >  2:7310604/1‑93 (MQ=255)

                              ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCaaaaaaaa                                                           <  2:7371332/93‑1 (MQ=255)

                              ccTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCaaaaaaaa                                                           <  1:115876/93‑1 (MQ=255)

                                taataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAagc                                                         >  1:3855561/1‑93 (MQ=255)

                                 aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTACCGCGCGATTGGGGCAAAAAAAagca                                                        >  2:2224412/1‑93 (MQ=255)

                                 aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAagca                                                        <  2:1177460/93‑1 (MQ=255)

                                 aataaGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAagca                                                        >  2:4737449/1‑93 (MQ=255)

                                   taagataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAagcagc                                                      <  2:4545805/93‑1 (MQ=255)

                                     agataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCgg                                                    >  1:5071313/1‑93 (MQ=255)

                                       ataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTa                                                  <  2:402306/93‑1 (MQ=255)

                                       ataagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGC‑AAAAAAAGCAGCGGTAc                                                 <  2:7417241/93‑1 (MQ=255)

                                        taagCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAc                                                 <  1:4197953/93‑1 (MQ=255)

                                          agCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgt                                               <  1:2268028/93‑1 (MQ=255)

                                           gCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTAcgtc                                              <  2:4204417/93‑1 (MQ=255)

                                                aCATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTAc                                         >  1:565723/1‑93 (MQ=255)

                                                aCATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTAc                                         >  2:4923004/1‑93 (MQ=255)

                                                aCATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTAc                                         <  1:4469756/93‑1 (MQ=255)

                                                   tcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgc                                      >  1:6415078/1‑93 (MQ=255)

                                                   tcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgc                                      >  1:3915018/1‑93 (MQ=255)

                                                   tcatcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGAGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgc                                      >  2:4312686/1‑93 (MQ=255)

                                                    catcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgct                                     <  1:301270/93‑1 (MQ=255)

                                                    catcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgct                                     >  2:443527/1‑93 (MQ=255)

                                                     atcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgctg                                    >  1:423089/1‑93 (MQ=255)

                                                      tcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgctgc                                   <  1:3195985/93‑1 (MQ=255)

                                                      tcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgctgc                                   <  2:1822011/93‑1 (MQ=255)

                                                      tcCTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCgctgc                                   >  1:7889144/1‑93 (MQ=255)

                                                       ccTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGTAGCGGTACGTCGTTACCgctgct                                  >  2:5654301/1‑93 (MQ=255)

                                                        ctcccACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                 <  2:5915297/90‑1 (MQ=255)

                                                        cTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                 >  2:2103722/1‑93 (MQ=255)

                                                        cTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                 >  1:3839035/1‑93 (MQ=255)

                                                        cTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                 >  2:2180009/1‑93 (MQ=255)

                                                        cTTCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGCTAACGCGCGATTGGGGCACAAAAAAGCAGCGGTACGTCGTTACCGctgctg                                 >  1:2413258/1‑93 (MQ=255)

                                                         ttCCACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTgg                                >  1:5411966/1‑93 (MQ=255)

                                                           ccACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGaa                              >  2:460602/1‑93 (MQ=255)

                                                           ccACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGaa                              <  1:7682354/93‑1 (MQ=255)

                                                            cACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAAt                             >  1:285744/1‑93 (MQ=255)

                                                            cACTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAAt                             >  1:6088569/1‑93 (MQ=255)

                                                             aCTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTCCTGGAATg                            >  2:1540985/1‑93 (MQ=255)

                                                              cTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGt                           >  1:2211458/1‑93 (MQ=255)

                                                              cTGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGt                           <  1:4278589/93‑1 (MQ=255)

                                                               tGTTGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGtt                          >  2:1045206/1‑93 (MQ=255)

                                                                 ttGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgc                        >  1:3979541/1‑93 (MQ=255)

                                                                 ttGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgc                        <  2:5734181/93‑1 (MQ=255)

                                                                  tGACCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTgcg                       <  2:6461092/93‑1 (MQ=255)

                                                                    aCCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAGAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGcc                     <  1:7453761/93‑1 (MQ=255)

                                                                    aCCATGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGcc                     <  1:7535345/93‑1 (MQ=255)

                                                                       aTGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCa                  <  2:127153/93‑1 (MQ=255)

                                                                       aTGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCa                  <  1:1047474/93‑1 (MQ=255)

                                                                       aTGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCa                  <  2:1964596/93‑1 (MQ=255)

                                                                        tGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCAc                 >  1:3389870/1‑93 (MQ=255)

                                                                        tGACAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCAc                 >  1:4293376/1‑93 (MQ=255)

                                                                        tGACAGGATGTTCAGTCGCCAGGAGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCAc                 >  2:7444771/1‑93 (MQ=255)

                                                                         gACAGGATTTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCTTTACCGCTGCTGGAATGTTGCGCCTCAcc                <  2:6212124/93‑1 (MQ=255)

                                                                           cAGGATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGt              <  2:5307023/93‑1 (MQ=255)

                                                                             ggATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTAt            <  1:874887/93‑1 (MQ=255)

                                                                              gATGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATc           >  1:7064728/1‑93 (MQ=255)

                                                                               aTGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCa          >  1:7335113/1‑93 (MQ=255)

                                                                               aTGTTCAGTCGCCAGGAGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCa          >  2:5221364/1‑93 (MQ=255)

                                                                                tGTTCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAg         >  1:7820138/1‑93 (MQ=255)

                                                                                  ttCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTT‑CCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTa      >  2:7762852/1‑93 (MQ=255)

                                                                                   tCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTa      <  2:5968459/93‑1 (MQ=255)

                                                                                   tCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTa      >  1:5749413/1‑93 (MQ=255)

                                                                                   tCAGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTa      <  1:1496605/93‑1 (MQ=255)

                                                                                     aGTCGCCAGGCGTTGACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa    <  1:6594219/93‑1 (MQ=255)

                                                                                     aGTCGCCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTaaa    <  2:4117172/93‑1 (MQ=255)

                                                                                      gtcgccAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAAc   >  2:6695742/1‑93 (MQ=255)

                                                                                      gtcgccAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAAc   <  1:3736928/93‑1 (MQ=255)

                                                                                       tcgccAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACa  <  2:1664184/93‑1 (MQ=255)

                                                                                       tcgccAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACa  <  1:10141/93‑1 (MQ=255)

                                                                                       tcgccAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACa  >  1:5834130/1‑93 (MQ=255)

                                                                                       tcgccAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACa  >  1:4532947/1‑93 (MQ=255)

CCGAAAACTGTACCGAGAACAACCAGGTAACCTAATAAGATAAGCACGACATCATCCTTCCACTGTTGACCATGACAGGATGTTCAGTCGTCAGGCGTTAACGCGCGATTGGGGCAAAAAAAAGCAGCGGTACGTCGTTACCGCTGCTGGAATGTTGCGCCTCACCGTATCAGTTAAACA  >  NC_000913/1975117‑1975296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`