Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,983,890 T→C T88A (ACT→GCT)  araF ← L‑arabinose‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,983,8900TC99.5% 667.2 / NA 215T88A (ACT→GCT) araFL‑arabinose‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (90/124);  total (91/124)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

GTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGTACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAA  >  NC_000913/1983801‑1983979
                                                                                         |                                                                                         
 ttAACAAGCTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACaa                                                                                       <  1:7203611/93‑1 (MQ=255)
 ttAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACaa                                                                                       >  1:6001009/1‑93 (MQ=255)
    aCAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATa                                                                                    <  1:7086703/93‑1 (MQ=255)
     cAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATaa                                                                                   <  1:895811/93‑1 (MQ=255)
     cAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATaa                                                                                   <  2:3139602/93‑1 (MQ=255)
      aaaCTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAc                                                                                  <  1:5472585/93‑1 (MQ=255)
       aaCTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACg                                                                                 >  1:4278042/1‑93 (MQ=255)
          tGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGaaa                                                                              <  1:2548028/93‑1 (MQ=255)
           ggTCATCCACGGCAATGACTTTCATATCGTAGCCACGCTCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAAc                                                                             <  2:4185250/93‑1 (MQ=255)
           ggTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAAc                                                                             <  2:4185248/93‑1 (MQ=255)
           ggTCACCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAAc                                                                             <  2:7823639/93‑1 (MQ=255)
               aTCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCttt                                                                         >  1:6299949/1‑93 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        <  2:7608846/93‑1 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        <  2:1775282/93‑1 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        <  1:3287005/93‑1 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        >  1:6678973/1‑93 (MQ=255)
                 ccACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTg                                                                       >  2:3623788/1‑93 (MQ=255)
                 ccACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTg                                                                       >  1:3574741/1‑93 (MQ=255)
                  cACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTgc                                                                      <  2:5062452/93‑1 (MQ=255)
                     ggCAATGACTTTGATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGcc                                                                   <  2:4988524/93‑1 (MQ=255)
                      gCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCa                                                                  <  1:5300314/93‑1 (MQ=255)
                       cAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCAc                                                                 <  1:5442500/93‑1 (MQ=255)
                         aTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTg                                                               <  1:3761343/93‑1 (MQ=255)
                         aTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTg                                                               >  1:3865556/1‑93 (MQ=255)
                         aTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTg                                                               <  2:2235899/93‑1 (MQ=255)
                         aTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTg                                                               <  1:7671544/93‑1 (MQ=255)
                           gACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGc                                                             >  2:3998907/1‑93 (MQ=255)
                            aCTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCa                                                            <  1:4980927/93‑1 (MQ=255)
                            aCTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCa                                                            <  1:208050/93‑1 (MQ=255)
                                tCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCa                                                        <  2:6431134/93‑1 (MQ=255)
                                tCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCa                                                        >  1:1959986/1‑93 (MQ=255)
                                tCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCa                                                        >  1:1809292/1‑93 (MQ=255)
                                 cATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAg                                                       >  1:7355663/1‑93 (MQ=255)
                                 cATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAg                                                       >  1:1197377/1‑93 (MQ=255)
                                 cATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAg                                                       <  1:3893605/93‑1 (MQ=255)
                                   tatCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGc                                                     <  2:6001416/93‑1 (MQ=255)
                                   tatCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGc                                                     >  2:5953871/1‑93 (MQ=255)
                                    atCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCt                                                    <  1:6930333/93‑1 (MQ=255)
                                    atCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCt                                                    >  2:6873019/1‑93 (MQ=255)
                                     tCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTg                                                   >  2:156422/1‑93 (MQ=255)
                                      cGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGt                                                  <  2:2061983/93‑1 (MQ=255)
                                        tAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCg                                                >  1:2144818/1‑93 (MQ=255)
                                        tAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCg                                                <  2:6330545/93‑1 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              <  1:5720769/93‑1 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              <  1:3981424/93‑1 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              >  1:4486411/1‑93 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACGGGCAGCCAGGCTGTCGAt                                              <  1:4795322/93‑1 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGGTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGTAGCCAAGCTGTCGac                                              >  2:6224553/1‑92 (MQ=255)
                                           ccACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATc                                             >  2:6118301/1‑93 (MQ=255)
                                           ccACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATc                                             <  2:5283165/93‑1 (MQ=255)
                                             aCGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGAAGCCCGGCTGtcgctcgc                                           >  2:2214480/1‑93 (MQ=255)
                                               gcgcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGt                                         <  2:260423/93‑1 (MQ=255)
                                               gcgcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGt                                         >  1:1733117/1‑93 (MQ=255)
                                               gcgcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGt                                         <  1:7221653/93‑1 (MQ=255)
                                                cgcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGtt                                        >  2:33908/1‑93 (MQ=255)
                                                 gcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTc                                       >  2:2797090/1‑93 (MQ=255)
                                                  cTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCa                                      >  1:3042694/1‑93 (MQ=255)
                                                  cTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCa                                      >  2:1879904/1‑93 (MQ=255)
                                                  cTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCa                                      <  2:3100278/93‑1 (MQ=255)
                                                   tttCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCaa                                     <  2:1207640/93‑1 (MQ=255)
                                                    ttCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAAt                                    >  1:4780725/1‑93 (MQ=255)
                                                        cgacgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGttt                                <  2:2677592/93‑1 (MQ=255)
                                                         gacgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttt                               >  1:6571197/1‑93 (MQ=255)
                                                         gacgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttt                               <  2:6523163/93‑1 (MQ=255)
                                                         gacgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttt                               >  1:6839162/1‑93 (MQ=255)
                                                          acgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGttttt                              <  1:4915562/93‑1 (MQ=255)
                                                          acgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGttttt                              <  1:2364991/93‑1 (MQ=255)
                                                          acgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGttttt                              >  2:4388724/1‑93 (MQ=255)
                                                           cgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttttt                             <  1:5944790/93‑1 (MQ=255)
                                                            gaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTc                            <  1:4636101/93‑1 (MQ=255)
                                                              tGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGc                          >  1:7621974/1‑93 (MQ=255)
                                                                 cAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCAt                       <  2:7179362/93‑1 (MQ=255)
                                                                  agagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCGGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATc                      >  1:7745238/1‑93 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     >  1:3598196/1‑93 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     >  1:4437979/1‑93 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     <  1:455003/93‑1 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCAAAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     <  2:6902996/93‑1 (MQ=255)
                                                                    agCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcg                    <  2:2391541/93‑1 (MQ=255)
                                                                    agCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcg                    <  2:4562214/93‑1 (MQ=255)
                                                                     gCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcgg                   <  2:2509344/93‑1 (MQ=255)
                                                                        gatgttGGGGTCCGGGGAACAAATAACCAACGCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcac                <  1:6632968/89‑1 (MQ=255)
                                                                        gAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcac                >  1:3743529/1‑93 (MQ=255)
                                                                        gAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcac                >  2:7502954/1‑93 (MQ=255)
                                                                          gTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTTGCCATCcggcacgg              >  2:802344/1‑93 (MQ=255)
                                                                           tttGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcacggc             <  1:7496255/93‑1 (MQ=255)
                                                                           tttGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcacggc             >  2:1052779/1‑93 (MQ=255)
                                                                           tttGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcacggc             >  1:3496996/1‑93 (MQ=255)
                                                                             tGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCaa           <  1:7724868/93‑1 (MQ=255)
                                                                              ggggTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAAt          >  2:4679500/1‑93 (MQ=255)
                                                                              ggggTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAAt          <  1:152284/93‑1 (MQ=255)
                                                                                 gTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCtt       <  1:2415570/93‑1 (MQ=255)
                                                                                  tCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTta      <  1:7760875/93‑1 (MQ=255)
                                                                                   ccGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaa     >  1:231155/1‑93 (MQ=255)
                                                                                    cGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaat    >  1:605611/1‑93 (MQ=255)
                                                                                    cGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaat    <  1:4054150/93‑1 (MQ=255)
                                                                                     ggAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaata   >  1:7255094/1‑93 (MQ=255)
                                                                                     ggAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaata   >  1:1321602/1‑93 (MQ=255)
                                                                                      gAGCACAAATAACGCACCCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaataa  <  1:583723/93‑1 (MQ=255)
                                                                                      gAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaataa  <  2:3879637/93‑1 (MQ=255)
                                                                                      gAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaataa  <  1:3255254/93‑1 (MQ=255)
                                                                                         |                                                                                         
GTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGTACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAA  >  NC_000913/1983801‑1983979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑