BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,011,752	T→C	S168P (TCC→CCC)	fliF →	FliF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,011,752	0	T	C	99.5%	616.4 / ‑5.2	199	S168P (TCC→CCC)	fliF	FliF
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (102/96); total (102/97)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 203 total aligned reads displayed.

TCTCGTACCATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAATCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGC  >  NC_000913/2011662‑2011841

 ctcGTACAATCGAACCTATCGGCCCAGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAAcccc                                                                                        <  2:1029490/93‑1 (MQ=255)

  tcGTACCATCGAACCTATCGGCCCGGTAGAAGGGGCGCGCGTACATCTGGCAATGCCGAACCCGTCTTTATTCATCCGTGAACAAAAACcccc                                                                                       <  1:7043796/93‑1 (MQ=255)

  tcGTACCATCGAAACTATCGGNCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACcccc                                                                                       <  1:6393331/93‑1 (MQ=255)

  tcGTACCATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACcccc                                                                                       <  1:7374266/93‑1 (MQ=255)

  tcGTACCATCGAAACAATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACcccc                                                                                       >  1:5785218/1‑93 (MQ=255)

   cGTACCATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCt                                                                                      >  1:4736254/1‑93 (MQ=255)

      aCCATCGAAACTATCGGCCCGGTTAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCt                                                                                   <  2:4295181/93‑1 (MQ=255)

       ccATCGAAACTATCGGCCCAGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTg                                                                                  >  2:4764939/1‑93 (MQ=255)

       ccATCGAAACTATCGGCCCAGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTg                                                                                  >  1:7257002/1‑93 (MQ=255)

        cATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGc                                                                                 <  1:4697260/93‑1 (MQ=255)

        cATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGc                                                                                 >  1:1352513/1‑93 (MQ=255)

          tCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCAt                                                                               >  1:5542567/1‑93 (MQ=255)

           cGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATc                                                                              <  1:6675920/93‑1 (MQ=255)

           cGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATc                                                                              <  1:2514494/93‑1 (MQ=255)

           cGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATGCGTCCGTGAACAAAAACCCCCTTCTGCATc                                                                              <  2:3717674/93‑1 (MQ=255)

           cGAAACTATCGGCACGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATc                                                                              <  1:7296411/93‑1 (MQ=255)

              aaCTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGt                                                                           <  2:3262912/93‑1 (MQ=255)

               aCTATCGGCCCGGGAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTg                                                                          <  1:7156228/93‑1 (MQ=255)

                cTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATCCGTCCGTTAACAAAAACCCCCTTCTGCATCGGTGa                                                                         <  1:2555582/93‑1 (MQ=255)

                 tATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGAc                                                                        >  2:3398187/1‑93 (MQ=255)

                 tATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGAc                                                                        >  1:6143552/1‑93 (MQ=255)

                 tATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGAc                                                                        >  1:2442280/1‑93 (MQ=255)

                 tATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGAc                                                                        <  2:4531539/93‑1 (MQ=255)

                 tATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTATTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGAc                                                                        >  1:2402023/1‑93 (MQ=255)

                  aTCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACg                                                                       >  2:7467658/1‑93 (MQ=255)

                  aTCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACg                                                                       >  1:692052/1‑93 (MQ=255)

                     ggCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTa                                                                    >  2:490063/1‑93 (MQ=255)

                     ggCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTa                                                                    <  2:4701802/93‑1 (MQ=255)

                      gCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTaa                                                                   >  2:3455165/1‑93 (MQ=255)

                      gCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTaa                                                                   >  2:6823272/1‑93 (MQ=255)

                      gCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTaa                                                                   >  2:6382448/1‑93 (MQ=255)

                       cccGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAACCCCCCTTCTGCATCGGTGACGGTaaa                                                                  <  2:4041146/93‑1 (MQ=255)

                       cccGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTaaa                                                                  <  1:7473353/93‑1 (MQ=255)

                         cGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATc                                                                <  2:4568018/93‑1 (MQ=255)

                         cGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATc                                                                <  1:2029408/93‑1 (MQ=255)

                         cGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATc                                                                >  1:7437442/1‑93 (MQ=255)

                         cGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATc                                                                >  1:3807162/1‑93 (MQ=255)

                            tAAAAGTGGCGCTCGTACATCGGGCAATGCCGAAACCGTCTTTATTCTTCCGTGAACAAAATCCCCCTTCTGCATCGGTGACGGTAAATCTGt                                                             <  2:1311810/93‑1 (MQ=255)

                            tAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGt                                                             <  2:3176416/93‑1 (MQ=255)

                            tAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGt                                                             <  2:7831551/93‑1 (MQ=255)

                             aaaaGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGtt                                                            <  2:6263927/93‑1 (MQ=255)

                             aaaaGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGtt                                                            >  1:7590675/1‑93 (MQ=255)

                              aaaGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTa                                                           <  1:1940512/93‑1 (MQ=255)

                               acggggCGCGCGGACCTCGGGCAATGCCGACACCGTCGTTAGTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTAc                                                          <  1:4076739/91‑1 (MQ=255)

                                aGGGGCGCGCGCACAACTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTccc                                                         <  1:2100215/93‑1 (MQ=255)

                                 ggggCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTAccc                                                        <  1:3489345/93‑1 (MQ=255)

                                   ggCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCgg                                                      >  1:4599637/1‑93 (MQ=255)

                                      gcgcgTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCcg                                                   <  2:5718404/93‑1 (MQ=255)

                                       cgcgTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCcgc                                                  <  1:1419334/93‑1 (MQ=255)

                                         cgTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCgcgc                                                <  1:1063925/93‑1 (MQ=255)

                                          gTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCa                                               <  1:7273853/93‑1 (MQ=255)

                                          gTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCa                                               <  1:2879311/93‑1 (MQ=255)

                                           tACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCAc                                              >  2:7172049/1‑93 (MQ=255)

                                           tACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCAc                                              >  2:2535040/1‑93 (MQ=255)

                                             cATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTc                                            >  1:984468/1‑93 (MQ=255)

                                             cATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTc                                            <  1:4334236/93‑1 (MQ=255)

                                              aTCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCg                                           >  1:3018843/1‑93 (MQ=255)

                                               tCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGa                                          <  2:760821/93‑1 (MQ=255)

                                               tCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGa                                          <  1:4382271/93‑1 (MQ=255)

                                               tCTGGCAATGCCGAAACCGCCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGa                                          <  2:3001697/93‑1 (MQ=255)

                                                cTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACTCGGCCGCGCACTCGAt                                         >  1:5049300/1‑93 (MQ=255)

                                                 tGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATg                                        >  1:3939152/1‑93 (MQ=255)

                                                 tGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCGGTTACCCGGCCGCGCACTCGATg                                        >  2:4345034/1‑93 (MQ=255)

                                                  ggCAATGTCCAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGa                                       <  2:1844535/93‑1 (MQ=255)

                                                   gCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGaa                                      <  1:1195930/93‑1 (MQ=255)

                                                       tGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGc                                  >  1:1768899/1‑93 (MQ=255)

                                                           gAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAAt                              >  1:1797005/1‑93 (MQ=255)

                                                           gAAACCGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAAt                              >  2:1811909/1‑93 (MQ=255)

                                                             aaCCGTCTTTATTCGTCCGTGAAC‑AAAACCCCCCTCTTGATTGGGGACGGGAAAACTGGTACCCCGCCGCGCACCCGGTGAAGGGGAAATTAg                           >  1:6331547/1‑93 (MQ=255)

                                                               ccGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGATAGGCAAATTAgc                          >  1:1911404/1‑93 (MQ=255)

                                                               ccGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAgc                          <  1:2663660/93‑1 (MQ=255)

                                                                cGTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAgcg                         >  1:7059755/1‑93 (MQ=255)

                                                                 gTCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAgcgc                        >  2:2865261/1‑93 (MQ=255)

                                                                 ctCTTTATTCGTCCGTGAACAAAACCCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAgcgc                        <  2:5514547/92‑1 (MQ=255)

                                                                  tCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGcc                       >  2:705909/1‑93 (MQ=255)

                                                                  tCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGcc                       >  2:5282806/1‑93 (MQ=255)

                                                                  tCTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGcc                       >  1:1097960/1‑93 (MQ=255)

                                                                   cTTTATTCGTCCGTGAACAAAAACCCCCTTCTGCATCCGTGACGGTAAATCTGTTACCCGGCTGCGCACTCGATGAAGGGCAAATTAGCGCCa                      >  1:7780686/1‑93 (MQ=255)

                                                                    tttATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCAt                     >  2:3417624/1‑93 (MQ=255)

                                                                      tATTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATtg                   >  1:3285452/1‑93 (MQ=255)

                                                                       aTTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATtgt                  >  2:7659491/1‑93 (MQ=255)

                                                                       aTTCGTCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATtgt                  <  1:3494951/93‑1 (MQ=255)

                                                                            tCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATc             >  1:21640/1‑93 (MQ=255)

                                                                            tCCGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATc             <  2:1416299/93‑1 (MQ=255)

                                                                             ccGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCt            <  1:272519/93‑1 (MQ=255)

                                                                             ccGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCt            <  1:479396/93‑1 (MQ=255)

                                                                              cGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTg           <  1:1437155/93‑1 (MQ=255)

                                                                              cGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTg           <  2:3876089/93‑1 (MQ=255)

                                                                              cGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTg           <  2:1045434/93‑1 (MQ=255)

                                                                              cGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTg           >  1:564452/1‑93 (MQ=255)

                                                                              cGTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTg           <  1:5948580/93‑1 (MQ=255)

                                                                               gTGAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTgg          >  2:5104431/1‑93 (MQ=255)

                                                                                 gAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGtt        >  2:5463353/1‑93 (MQ=255)

                                                                                 gAACAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGtt        <  2:2905812/93‑1 (MQ=255)

                                                                                    cAAAAACCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTcc     <  2:2718414/93‑1 (MQ=255)

                                                                                     aaaaaCCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCa    <  2:5192119/93‑1 (MQ=255)

                                                                                     aaaaaCCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCa    <  2:6064846/93‑1 (MQ=255)

                                                                                      aaaaCCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCac   >  2:5341408/1‑92 (MQ=255)

                                                                                      aaaaCCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAg   >  2:6609303/1‑93 (MQ=255)

                                                                                      aaaaCCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAg   <  2:7366952/93‑1 (MQ=255)

TCTCGTACCATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCTTTATTCGTCCGTGAACAAAAATCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGC  >  NC_000913/2011662‑2011841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`