BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,879	A→G	N651S (AAC→AGC)	yaeT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,879	0	A	G	99.6%	874.7 / ‑5.4	280	N651S (AAC→AGC)	yaeT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (138/141); total (139/141)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 290 total aligned reads displayed.

TCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTA  >  NC_000913/199789‑199968

tcgatGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCt                                                                                         >  2:5370760/1‑93 (MQ=255)

    tGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGTCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTa                                                                                     >  1:7654741/1‑93 (MQ=255)

    tGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTa                                                                                     <  2:5874684/93‑1 (MQ=255)

     gACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTAt                                                                                    >  2:1028556/1‑93 (MQ=255)

      aCGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATg                                                                                   >  1:6282684/1‑93 (MQ=255)

        gATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGcc                                                                                 <  1:3970738/93‑1 (MQ=255)

          tCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCgg                                                                               >  2:2374406/1‑93 (MQ=255)

          tCACAAATGGGTTGTTCTGGGGCGTACCCACTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCgg                                                                               >  2:4531552/1‑93 (MQ=255)

           cacaAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCggt                                                                              <  2:6797605/93‑1 (MQ=255)

           cacaAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCggt                                                                              >  1:1729230/1‑93 (MQ=255)

            acaAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCggtg                                                                             <  1:5209817/93‑1 (MQ=255)

             caAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCggtgg                                                                            >  1:391836/1‑93 (MQ=255)

             caAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGGGATGGTTTAGGCGGCAAAGAGAGGCCGTTCTACGAGAGCTTCTATGCCggtgg                                                                            >  1:6897130/1‑93 (MQ=255)

                aTGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTTGGCGGCAAAGAGATGCCGTTCTACGAGTGCTTCTATGCCGGTGGTTc                                                                         >  2:7027818/1‑93 (MQ=255)

                aTGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTc                                                                         <  2:1239445/93‑1 (MQ=255)

                aTGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTc                                                                         <  2:1898328/93‑1 (MQ=255)

                 tGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTcc                                                                        >  2:386797/1‑93 (MQ=255)

                  gggTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCa                                                                       <  2:5556858/93‑1 (MQ=255)

                  gggTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCa                                                                       <  2:905960/93‑1 (MQ=255)

                  gggTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCa                                                                       <  1:2822537/93‑1 (MQ=255)

                  gggTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGCGGTTCCa                                                                       >  1:7531826/1‑93 (MQ=255)

                   ggTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAg                                                                      <  2:2315694/93‑1 (MQ=255)

                      tgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCAc                                                                   <  2:6065658/93‑1 (MQ=255)

                      tgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCAc                                                                   <  1:4533118/93‑1 (MQ=255)

                      tgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCAc                                                                   >  1:4672123/1‑93 (MQ=255)

                      tgttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCAc                                                                   <  1:2024089/93‑1 (MQ=255)

                       gttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCGACGAGAGCTTCTATGCCGGTGGTTCCAGCaac                                                                  >  2:1150835/1‑91 (MQ=255)

                        ttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcg                                                                 >  2:5439419/1‑93 (MQ=255)

                        ttCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcg                                                                 >  1:4955424/1‑93 (MQ=255)

                        gtCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcg                                                                 <  1:3671602/92‑1 (MQ=255)

                          cTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcgtg                                                               >  2:2207882/1‑93 (MQ=255)

                            ggggCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcgtgcg                                                             <  2:7154249/93‑1 (MQ=255)

                            ggggCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcgtgcg                                                             >  2:3363544/1‑93 (MQ=255)

                             gggCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcgtgcgt                                                            <  1:1008934/93‑1 (MQ=255)

                              ggCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACcgtgcgtg                                                           <  2:4824619/93‑1 (MQ=255)

                                  tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCTTGCGTGGCtt                                                       >  1:4712770/1‑93 (MQ=255)

                                  tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCtt                                                       >  1:4010548/1‑93 (MQ=255)

                                  tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCtt                                                       >  1:5609568/1‑93 (MQ=255)

                                  tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCtt                                                       >  1:4347508/1‑93 (MQ=255)

                                  tACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGGTCCAGCACCGTGCGTGGCtt                                                       >  1:5082804/1‑93 (MQ=255)

                                   aCCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTc                                                      <  2:2021182/93‑1 (MQ=255)

                                    cccGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGAGGCAGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCttac                                                     >  2:7891580/1‑91 (MQ=255)

                                     ccGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCa                                                    <  1:6838870/93‑1 (MQ=255)

                                       gCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGt                                                  <  1:5945449/93‑1 (MQ=255)

                                          ggggTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCa                                               <  2:795349/93‑1 (MQ=255)

                                            ggTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAAt                                             >  1:6101966/1‑93 (MQ=255)

                                             gTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATa                                            <  2:5848150/93‑1 (MQ=255)

                                              ttATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCAACGTGCGTGGCTTCCAGTCCAATAc                                           >  2:5173384/1‑93 (MQ=255)

                                                aTGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGCTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCa                                         >  2:5727195/1‑93 (MQ=255)

                                                 tGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCAt                                        <  2:145634/93‑1 (MQ=255)

                                                  ggTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCAtt                                       <  1:2975685/93‑1 (MQ=255)

                                                  ggTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCAtt                                       >  1:927441/1‑93 (MQ=255)

                                                    tGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTgg                                     <  2:2533599/93‑1 (MQ=255)

                                                    tGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTgg                                     >  2:3586676/1‑93 (MQ=255)

                                                    tGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTgg                                     >  2:1318487/1‑93 (MQ=255)

                                                     gATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGt                                    <  1:266563/93‑1 (MQ=255)

                                                      aTGGTTTAGGCGGCAAAGAGATGCCGGTCTAAGAGAGCTTCTATGCCGGTGGTTCCAGCCCCGTGCGTGGCTTCCAGTCCAATACCATTGGTc                                   <  1:5401678/93‑1 (MQ=255)

                                                       tGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTcc                                  >  2:3085198/1‑93 (MQ=255)

                                                         gTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGa                                >  2:2683087/1‑93 (MQ=255)

                                                           ttAGGCGGCAAAGAGATGCCGTTGTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATCCCATTGGTCCGaaa                              <  2:4332784/93‑1 (MQ=255)

                                                           ttAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGaaa                              <  1:3726399/93‑1 (MQ=255)

                                                           ttAGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGaaa                              <  1:1288721/93‑1 (MQ=255)

                                                             aGGCGGCAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGc                            <  1:663801/93‑1 (MQ=255)

                                                              ggcggcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCa                           <  1:5334282/93‑1 (MQ=255)

                                                              ggcggcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCa                           >  2:1818064/1‑93 (MQ=255)

                                                                cggcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGt                         <  2:296059/93‑1 (MQ=255)

                                                                cggcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGt                         <  1:1047528/93‑1 (MQ=255)

                                                                 ggcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGATAGCAGtt                        <  2:7761077/93‑1 (MQ=255)

                                                                 ggcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGtt                        <  1:7562633/93‑1 (MQ=255)

                                                                  gcAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGttt                       <  1:5868078/93‑1 (MQ=255)

                                                                   cAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTGCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTa                      >  2:3630240/1‑93 (MQ=255)

                                                                   cAAAGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTa                      <  2:2868156/93‑1 (MQ=255)

                                                                    aaaGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTAc                     <  2:6551146/93‑1 (MQ=255)

                                                                     aaGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACt                    <  2:4065383/93‑1 (MQ=255)

                                                                     aaGAGATGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACt                    >  2:5027023/1‑93 (MQ=255)

                                                                       gagaTGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTc                  >  1:7046307/1‑93 (MQ=255)

                                                                        agaTGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTcc                 <  2:76090/93‑1 (MQ=255)

                                                                         gaTGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTccg                >  2:4943469/1‑92 (MQ=255)

                                                                         gaTGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTccc                >  1:2382866/1‑93 (MQ=255)

                                                                         gaTGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTccc                >  1:1908154/1‑93 (MQ=255)

                                                                         gaTGCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTccc                >  1:2228253/1‑93 (MQ=255)

                                                                            gCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCa             <  1:3152387/93‑1 (MQ=255)

                                                                            gCCGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCa             >  1:1652130/1‑93 (MQ=255)

                                                                              cGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATc           <  2:172424/93‑1 (MQ=255)

                                                                              cGTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATc           <  2:3533558/93‑1 (MQ=255)

                                                                               gTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATcg          >  2:734877/1‑92 (MQ=255)

                                                                               gTTCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCa          >  2:2078795/1‑93 (MQ=255)

                                                                               attCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCa          <  1:2504943/92‑1 (MQ=255)

                                                                                ttCTACGAGAGCTTCTGTGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAg         >  2:7508778/1‑93 (MQ=255)

                                                                                ttCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAg         >  2:2466686/1‑93 (MQ=255)

                                                                                ttCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAg         >  1:7370684/1‑93 (MQ=255)

                                                                                 tCTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAgg        >  1:342262/1‑93 (MQ=255)

                                                                                  cTACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGc       <  1:7309549/93‑1 (MQ=255)

                                                                                   tACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGcc      >  1:115528/1‑93 (MQ=255)

                                                                                   tACGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGcc      >  2:6220145/1‑93 (MQ=255)

                                                                                    aCGAGAGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCa     <  1:4854316/93‑1 (MQ=255)

                                                                                      gagaGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGt   <  1:2429190/93‑1 (MQ=255)

                                                                                      gagaGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGt   <  1:7195782/93‑1 (MQ=255)

                                                                                      gagaGCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGt   <  1:5783926/93‑1 (MQ=255)

                                                                                       agagCTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTa  <  1:6269067/93‑1 (MQ=255)

TCGATGACGATCACAAATGGGTTGTTCTGGGGCGTACCCGCTGGGGTTATGGTGATGGTTTAGGCGGCAAAGAGATGCCGTTCTACGAGAACTTCTATGCCGGTGGTTCCAGCACCGTGCGTGGCTTCCAGTCCAATACCATTGGTCCGAAAGCAGTTTACTTCCCGCATCAGGCCAGTA  >  NC_000913/199789‑199968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`