BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,066,300	T→C	intergenic (‑957/‑330)	trs56 ← / → b1995	IS5 transposase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,066,300	0	T	C	100.0%	824.6 / NA	256	intergenic (‑957/‑330)	trs56/b1995	IS5 transposase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (115/141); total (115/141)

Only 100 of 257 total aligned reads displayed.

TCTGGCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGAC  >  NC_000913/2066210‑2066389

tctGGCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCt                                                                                         <  2:87564/93‑1 (MQ=255)

tctGGCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCt                                                                                         <  1:3993809/93‑1 (MQ=255)

  tGGCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTGAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTa                                                                                       <  1:1628154/93‑1 (MQ=255)

  tGGCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTa                                                                                       >  2:4637637/1‑93 (MQ=255)

    gCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTAcc                                                                                     >  1:2553705/1‑93 (MQ=255)

      cATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGc                                                                                   >  1:2741734/1‑93 (MQ=255)

       aTTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCa                                                                                  >  1:2157626/1‑93 (MQ=255)

        ttAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCat                                                                                 >  1:5025497/1‑93 (MQ=255)

        ttAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCat                                                                                 <  2:6588394/93‑1 (MQ=255)

         tAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCata                                                                                <  2:4422860/93‑1 (MQ=255)

         tAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCata                                                                                <  2:2699773/93‑1 (MQ=255)

           gagTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCAtata                                                                              >  1:6625416/1‑93 (MQ=255)

           gagTAACATTCTGCATTCATCCTTTTGAAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCAtcta                                                                              <  2:7019249/93‑1 (MQ=255)

            agTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATAc                                                                             <  2:4475302/93‑1 (MQ=255)

             gTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACg                                                                            <  2:669685/93‑1 (MQ=255)

              tAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACgg                                                                           >  1:7124383/1‑93 (MQ=255)

               aaCCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGt                                                                          >  2:3636148/1‑93 (MQ=255)

                 ccTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCa                                                                        <  1:1105053/93‑1 (MQ=255)

                  cTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCaa                                                                       >  1:1513783/1‑93 (MQ=255)

                   ttCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAAt                                                                      >  1:2757521/1‑93 (MQ=255)

                   ttCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAAt                                                                      >  1:5440966/1‑93 (MQ=255)

                    tCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAAtt                                                                     <  2:5300866/93‑1 (MQ=255)

                    tCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAAtt                                                                     >  2:1034652/1‑93 (MQ=255)

                     cTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAAttt                                                                    <  2:2122498/93‑1 (MQ=255)

                      tGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTc                                                                   <  2:5116528/93‑1 (MQ=255)

                      tGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTc                                                                   <  2:2904437/93‑1 (MQ=255)

                       gCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCa                                                                  <  1:195576/93‑1 (MQ=255)

                        cATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAg                                                                 >  2:2595450/1‑93 (MQ=255)

                         aTTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGc                                                                >  2:6701881/1‑93 (MQ=255)

                          ttCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCa                                                               >  1:4105821/1‑93 (MQ=255)

                          ttCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCa                                                               >  1:801188/1‑93 (MQ=255)

                             aTCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaatt                                                            >  2:3431221/1‑93 (MQ=255)

                             aTCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaatt                                                            <  2:6370281/93‑1 (MQ=255)

                              tCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaatta                                                           >  1:5354117/1‑93 (MQ=255)

                                cTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaattaat                                                         <  2:1064359/93‑1 (MQ=255)

                                 ttttGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaattaatt                                                        >  2:1305908/1‑93 (MQ=255)

                                 ttttGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaattaatt                                                        <  1:899634/93‑1 (MQ=255)

                                 ttttGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCaattaatt                                                        >  2:7219583/1‑93 (MQ=255)

                                  tttGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAattaatta                                                       >  2:7140555/1‑93 (MQ=255)

                                  tttGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAattaatta                                                       <  2:1396098/93‑1 (MQ=255)

                                  tttGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAattaatta                                                       <  1:5819792/93‑1 (MQ=255)

                                     gTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACAt                                                    >  2:4555550/1‑93 (MQ=255)

                                      tAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACAtt                                                   <  2:1640217/93‑1 (MQ=255)

                                       aaaaaGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTa                                                  <  1:1410419/93‑1 (MQ=255)

                                        aaaaGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTAt                                                 >  1:4405161/1‑93 (MQ=255)

                                         aaaGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATc                                                >  1:627050/1‑93 (MQ=255)

                                          aaGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATcc                                               <  1:1326089/93‑1 (MQ=255)

                                           aGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCa                                              <  2:5039595/93‑1 (MQ=255)

                                                atatTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCa                                         >  2:5811530/1‑93 (MQ=255)

                                                  atTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCaaa                                       <  1:4231809/93‑1 (MQ=255)

                                                  atTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCaaa                                       >  2:2230438/1‑93 (MQ=255)

                                                  atTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCaaa                                       <  1:7128769/93‑1 (MQ=255)

                                                    tAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGt                                     >  1:3412853/1‑93 (MQ=255)

                                                    tAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGt                                     <  1:7336695/93‑1 (MQ=255)

                                                    tAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGt                                     >  1:2266653/1‑93 (MQ=255)

                                                    tAGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGt                                     >  1:4747352/1‑93 (MQ=255)

                                                     aGTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTa                                    <  1:3266614/93‑1 (MQ=255)

                                                      gTATCAGCAATTAACCGGACCTGATACTTATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTAt                                   >  1:2611927/1‑93 (MQ=255)

                                                      gTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTAt                                   <  1:2515470/93‑1 (MQ=255)

                                                      gTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTAt                                   >  2:5442560/1‑93 (MQ=255)

                                                      gTATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTAt                                   >  2:2791372/1‑93 (MQ=255)

                                                       tATCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTAtt                                  <  1:7794556/93‑1 (MQ=255)

                                                       tATCAGCAATTAACCGGACCTGATACTGATATGAGCCTCACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTAtt                                  <  1:6899255/93‑1 (MQ=255)

                                                         tCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTg                                <  1:1670932/93‑1 (MQ=255)

                                                         tCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTg                                <  1:7657770/93‑1 (MQ=255)

                                                         tCAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTg                                >  2:7724363/1‑93 (MQ=255)

                                                          cAGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGt                               >  1:85151/1‑93 (MQ=255)

                                                           aGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGtc                              <  1:6147484/93‑1 (MQ=255)

                                                           aGCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGtc                              <  1:582988/93‑1 (MQ=255)

                                                            gCAATTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGtca                             <  1:7764732/93‑1 (MQ=255)

                                                              aaTTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGtcatc                           <  1:4705474/93‑1 (MQ=255)

                                                               aTTAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGtcatca                          >  1:3889352/1‑93 (MQ=255)

                                                                ttAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATcac                         >  2:5995666/1‑93 (MQ=255)

                                                                ttAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATcac                         <  2:566961/93‑1 (MQ=255)

                                                                ttAACCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATcac                         <  2:5921992/93‑1 (MQ=255)

                                                                  aaCCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACaa                       <  2:6937691/93‑1 (MQ=255)

                                                                  aaCCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACaa                       <  2:198424/93‑1 (MQ=255)

                                                                   aCCGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAat                      <  2:7091437/93‑1 (MQ=255)

                                                                   aCCGGACCTGATACTGATATGAGCCTTACCGCATACACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAat                      <  1:2482009/93‑1 (MQ=255)

                                                                    ccGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatg                     >  2:326854/1‑93 (MQ=255)

                                                                     cGGACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatga                    >  2:5589150/1‑93 (MQ=255)

                                                                      ggACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatgat                   <  1:1860706/93‑1 (MQ=255)

                                                                      ggACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatgat                   <  1:5716737/93‑1 (MQ=255)

                                                                      ggACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatgat                   <  1:5779025/93‑1 (MQ=255)

                                                                      ggACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatgat                   <  2:4183516/93‑1 (MQ=255)

                                                                       gACCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAatgatg                  <  2:3820432/93‑1 (MQ=255)

                                                                        aCCTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATgg                 <  2:731575/93‑1 (MQ=255)

                                                                         ccTGATACTGATATGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGt                >  2:7493265/1‑93 (MQ=255)

                                                                           tgatactgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTAc              <  2:4689121/93‑1 (MQ=255)

                                                                            gatactgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTAct             >  1:6487586/1‑92 (MQ=255)

                                                                             atactgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACct            >  2:5962669/1‑93 (MQ=255)

                                                                               tctgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACcttc          <  2:6247967/92‑1 (MQ=255)

                                                                               actgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACcttc          >  1:7786188/1‑93 (MQ=255)

                                                                               actgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACcttc          <  2:4913960/93‑1 (MQ=255)

                                                                                ctgataTGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACcttct         >  1:5043800/1‑93 (MQ=255)

                                                                                   atatGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTc      <  1:776711/93‑1 (MQ=255)

                                                                                    tatGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCa     <  2:4166990/93‑1 (MQ=255)

                                                                                      tGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGa   <  1:7507427/93‑1 (MQ=255)

                                                                                      tGAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGa   >  2:7864122/1‑93 (MQ=255)

                                                                                       gAGCCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGac  <  1:523338/93‑1 (MQ=255)

TCTGGCCATTAGAGTAACCTTCTGCATTCATCCTTTTGTAAAAAGTTTATATTAGTATCAGCAATTAACCGGACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGAC  >  NC_000913/2066210‑2066389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`