BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	200,658	T→C	R59R (CGT→CGC)	hlpA →	histone‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	200,658	0	T	C	99.3%	924.5 / ‑3.6	293	R59R (CGT→CGC)	hlpA	histone‑like protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (139/152); total (140/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

Only 100 of 297 total aligned reads displayed.

GGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGC  >  NC_000913/200568‑200747

gggCAGCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCAt                                                                                         <  1:4469232/93‑1 (MQ=255)

gggCAGCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCAt                                                                                         >  2:4412526/1‑93 (MQ=255)

  gCAGCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATgg                                                                                       <  2:6237076/93‑1 (MQ=255)

   cAGCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGa                                                                                      >  1:7929915/1‑93 (MQ=255)

    aGCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGaa                                                                                     <  2:7007223/93‑1 (MQ=255)

     gCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGaaa                                                                                    >  2:1265856/1‑93 (MQ=255)

     gCCTGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGaaa                                                                                    >  1:4835825/1‑93 (MQ=255)

        tGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCg                                                                                 >  1:1721684/1‑93 (MQ=255)

        tGTTCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCg                                                                                 >  1:3052982/1‑93 (MQ=255)

          ttCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGAt                                                                               >  2:7640798/1‑93 (MQ=255)

           tCCAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATc                                                                              <  2:3673360/93‑1 (MQ=255)

            ccAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCt                                                                             <  2:3330560/93‑1 (MQ=255)

            ccAGCGGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCt                                                                             <  1:1081786/93‑1 (MQ=255)

              agcggGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGc                                                                           <  2:2124704/93‑1 (MQ=255)

               gcgggTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCa                                                                          <  2:4486420/90‑1 (MQ=255)

               gcgggTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCa                                                                          <  2:774676/90‑1 (MQ=255)

               gcgggTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGGTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAAGCGATCTGCa                                                                          >  2:3184449/4‑93 (MQ=255)

                cgggTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAg                                                                         <  1:7748715/91‑1 (MQ=255)

                cgggTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAg                                                                         <  1:1375527/91‑1 (MQ=255)

                 gggTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAgg                                                                        >  1:1443497/2‑93 (MQ=255)

                    tAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTa                                                                     <  1:5374657/93‑1 (MQ=255)

                      gcgcAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTaaa                                                                   <  2:5796539/93‑1 (MQ=255)

                       ggcAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTaaaa                                                                  <  1:6738871/92‑1 (MQ=255)

                       cgcAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTaaaa                                                                  <  1:5440382/93‑1 (MQ=255)

                        gcAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAAt                                                                 <  1:2043341/93‑1 (MQ=255)

                          aGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGa                                                               >  1:5780860/1‑93 (MQ=255)

                           gAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaa                                                              >  2:1373055/1‑93 (MQ=255)

                           gAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaa                                                              >  2:5653960/1‑93 (MQ=255)

                            aaaaCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaa                                                             >  1:5900451/1‑93 (MQ=255)

                            aaaaCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaa                                                             <  2:1454741/93‑1 (MQ=255)

                            aaaaCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaa                                                             <  1:936227/93‑1 (MQ=255)

                             aaaCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaaa                                                            <  2:3099033/93‑1 (MQ=255)

                             aaaCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaaa                                                            <  1:6381570/93‑1 (MQ=255)

                             aaaCCGGTGGTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaaa                                                            >  2:7805177/1‑93 (MQ=255)

                              aaCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGaaaaa                                                           >  2:3814764/1‑93 (MQ=255)

                               aCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAg                                                          >  1:1946919/1‑93 (MQ=255)

                               aCCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAg                                                          >  1:21650/1‑93 (MQ=255)

                                ccGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGc                                                         <  1:2282278/93‑1 (MQ=255)

                                ccGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGc                                                         >  1:3391332/1‑93 (MQ=255)

                                 cGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCt                                                        <  1:6183481/93‑1 (MQ=255)

                                 cGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCt                                                        >  2:35893/1‑93 (MQ=255)

                                  ggTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTg                                                       >  1:6936194/1‑93 (MQ=255)

                                   gtgtTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGc                                                      >  1:2975571/1‑93 (MQ=255)

                                   gtgtTTCTAACACGCTAGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGc                                                      <  1:6414409/93‑1 (MQ=255)

                                    tgtTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCa                                                     <  1:3570367/93‑1 (MQ=255)

                                      tttCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGt                                                   <  2:5614523/93‑1 (MQ=255)

                                      tttCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGt                                                   >  1:7480671/1‑93 (MQ=255)

                                      tttCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGt                                                   >  2:7896246/1‑93 (MQ=255)

                                      tttCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGt                                                   >  1:6588432/1‑93 (MQ=255)

                                        tCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTcc                                                 <  1:4904716/93‑1 (MQ=255)

                                         cTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCa                                                <  1:1877109/93‑1 (MQ=255)

                                         cTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCa                                                <  1:7230056/93‑1 (MQ=255)

                                          tAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCAt                                               <  2:6641366/93‑1 (MQ=255)

                                                gCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGc                                         <  2:3185386/93‑1 (MQ=255)

                                                 cTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCg                                        <  2:5250843/93‑1 (MQ=255)

                                                  tGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCgg                                       <  2:2200757/93‑1 (MQ=255)

                                                  tGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCT‑AAATGAAAAAGCTGCAGTCCATGAAAGCggg                                      >  2:2992615/1‑93 (MQ=255)

                                                   ggAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCggg                                      >  2:5988468/1‑93 (MQ=255)

                                                    gAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGc                                     >  1:3705637/1‑93 (MQ=255)

                                                    gAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGc                                     <  2:2579120/93‑1 (MQ=255)

                                                    gAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGc                                     <  2:5446524/93‑1 (MQ=255)

                                                     aaaaTGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCa                                    >  2:5550325/1‑93 (MQ=255)

                                                      aaaTGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAg                                   >  1:7090946/1‑93 (MQ=255)

                                                       aaTGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGc                                  >  1:1506705/1‑93 (MQ=255)

                                                         tGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGa                                <  2:4895279/93‑1 (MQ=255)

                                                          gAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGAt                               <  2:1006108/93‑1 (MQ=255)

                                                          gAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGAt                               <  1:4659526/93‑1 (MQ=255)

                                                          gAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGAt                               >  1:2679256/1‑93 (MQ=255)

                                                          gAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGAt                               <  1:6024396/93‑1 (MQ=255)

                                                           aGTTCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATc                              <  1:3942026/93‑1 (MQ=255)

                                                             ttCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGc                            >  1:2827215/1‑93 (MQ=255)

                                                              tCAAAGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCa                           >  1:700531/1‑93 (MQ=255)

                                                                 aaGGCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTa                        >  2:4314650/1‑93 (MQ=255)

                                                                    gCCGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGc                     <  2:3950674/93‑1 (MQ=255)

                                                                      cGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTg                   >  2:7501922/1‑93 (MQ=255)

                                                                      cGTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTg                   <  2:1833910/93‑1 (MQ=255)

                                                                       gTGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTgg                  >  2:1613699/1‑93 (MQ=255)

                                                                        tGCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGa                 <  1:5108146/93‑1 (MQ=255)

                                                                         gCCAGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGCCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGaa                <  1:6484953/93‑1 (MQ=255)

                                                                            aGCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGaaaaa             >  1:2408667/1‑93 (MQ=255)

                                                                             gCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAg            >  2:6417864/1‑93 (MQ=255)

                                                                             gCGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAg            <  2:1960055/93‑1 (MQ=255)

                                                                              cGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGa           <  1:4174959/93‑1 (MQ=255)

                                                                              cGAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGa           <  1:7828513/93‑1 (MQ=255)

                                                                               gAACTGCAGCGCATGGAAACGGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGAc          <  2:7297039/93‑1 (MQ=255)

                                                                               gAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGAc          <  2:869704/93‑1 (MQ=255)

                                                                               gAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGAc          >  2:5928607/1‑93 (MQ=255)

                                                                               gAACTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGAc          >  2:1671864/1‑93 (MQ=255)

                                                                                 aCTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACgg        >  2:1104288/1‑92 (MQ=255)

                                                                                 aCTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGt        <  1:7652381/93‑1 (MQ=255)

                                                                                  cTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTg       >  2:4211032/1‑93 (MQ=255)

                                                                                  cTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTg       >  2:2238338/1‑93 (MQ=255)

                                                                                  cTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTg       <  2:7783307/93‑1 (MQ=255)

                                                                                  cTGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTg       >  1:4367102/1‑93 (MQ=255)

                                                                                   tGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGa      >  2:2740540/1‑93 (MQ=255)

                                                                                   tGCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGa      >  1:1656217/1‑93 (MQ=255)

                                                                                    gCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGAt     >  2:7359450/1‑93 (MQ=255)

                                                                                    gCAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCCGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGAt     <  2:654257/93‑1 (MQ=255)

                                                                                     cAGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATg    <  1:4239211/93‑1 (MQ=255)

                                                                                      aGCGCATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATgg   <  2:1217794/93‑1 (MQ=255)

GGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGC  >  NC_000913/200568‑200747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`