BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,874	T→C	L50L (TTA→TTG)	b2084 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,874	0	T	C	99.3%	864.6 / NA	273	L50L (TTA→TTG)	b2084	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (154/117); minor base A (1/1); total (155/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963

tCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCaa                                                                                         >  1:392713/1‑93 (MQ=255)

tCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCaa                                                                                         <  2:5455870/93‑1 (MQ=255)

 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTCCACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  1:3385701/93‑1 (MQ=255)

 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        >  2:3632871/1‑93 (MQ=255)

 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  1:550575/93‑1 (MQ=255)

 ccGTGTAAAGATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  1:6645481/93‑1 (MQ=255)

     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                    <  1:115047/93‑1 (MQ=255)

      tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCt                                                                                   >  2:4515040/1‑93 (MQ=255)

      tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCt                                                                                   >  1:6142024/1‑93 (MQ=255)

       aaaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCtt                                                                                  <  2:1477981/93‑1 (MQ=255)

        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 <  1:4920801/93‑1 (MQ=255)

        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 >  1:1236208/1‑93 (MQ=255)

          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                               <  1:7269135/93‑1 (MQ=255)

           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAt                                                                              <  1:3231857/93‑1 (MQ=255)

            tCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAtt                                                                             >  1:7786394/1‑93 (MQ=255)

              tGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGa                                                                           >  2:404183/1‑93 (MQ=255)

               gCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAc                                                                          >  2:6911488/1‑93 (MQ=255)

               gCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAc                                                                          <  1:6001028/93‑1 (MQ=255)

                cccGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAcc                                                                         >  2:1688868/1‑93 (MQ=255)

                      cGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCg                                                                   >  2:3879793/1‑93 (MQ=255)

                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 >  2:6212903/1‑93 (MQ=255)

                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 >  1:5643876/1‑93 (MQ=255)

                         tAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATa                                                                <  1:4096227/93‑1 (MQ=255)

                         tAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATa                                                                >  2:2128403/1‑93 (MQ=255)

                          aGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAg                                                               <  1:2976320/93‑1 (MQ=255)

                          aGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAg                                                               >  1:5124024/1‑93 (MQ=255)

                           gCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                              >  1:3655733/1‑93 (MQ=255)

                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  1:2477190/1‑93 (MQ=255)

                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  2:4317652/1‑93 (MQ=255)

                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                           <  2:4264656/93‑1 (MQ=255)

                               ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAGtcc                                                          >  1:6235430/1‑93 (MQ=255)

                               agCTCATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATACGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcc                                                          <  1:4352066/92‑1 (MQ=255)

                                  tGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctcc                                                       <  1:3492657/93‑1 (MQ=255)

                                  tGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctcc                                                       <  2:7496187/93‑1 (MQ=255)

                                   gATGGAGAACTCTGCTACACGGGCTTATTGTTGTATGCATAAGCCCTATCTCTGGCAACCGTCTGCCAGCGACCACAGCGATAGAATcctcct                                                      >  1:7027721/1‑93 (MQ=255)

                                     tGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTc                                                    <  2:7203116/93‑1 (MQ=255)

                                      ggAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCa                                                   <  1:6570440/93‑1 (MQ=255)

                                      ggAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCa                                                   >  2:6737685/1‑93 (MQ=255)

                                       gagaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAACACATCGATAGAATCCTCCTTCAt                                                  <  1:1062202/93‑1 (MQ=255)

                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 <  2:7178219/93‑1 (MQ=255)

                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 >  2:4863497/1‑93 (MQ=255)

                                            ctctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCAc                                             <  1:6297105/93‑1 (MQ=255)

                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                           <  2:5533150/93‑1 (MQ=255)

                                                gCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATg                                         <  1:2026025/93‑1 (MQ=255)

                                                   acacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCt                                      <  2:4730718/93‑1 (MQ=255)

                                                    cacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCtt                                     <  2:548662/93‑1 (MQ=255)

                                                    cacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCtt                                     <  2:3581886/93‑1 (MQ=255)

                                                     acGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCttt                                    <  2:5426394/93‑1 (MQ=255)

                                                     acGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCttt                                    >  1:2785874/1‑93 (MQ=255)

                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   <  1:2190413/93‑1 (MQ=255)

                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   >  2:5808407/1‑93 (MQ=255)

                                                       gggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCa                                  >  1:3170205/1‑93 (MQ=255)

                                                         gCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACt                                <  2:732737/93‑1 (MQ=255)

                                                          cTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACtt                               <  2:3400745/93‑1 (MQ=255)

                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              >  1:7187657/1‑93 (MQ=255)

                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              <  2:2869979/93‑1 (MQ=255)

                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              <  1:2345141/93‑1 (MQ=255)

                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            >  2:4626838/1‑93 (MQ=255)

                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           >  2:517238/1‑93 (MQ=255)

                                                                tttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGc                         >  2:1550668/1‑93 (MQ=255)

                                                                  tttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAt                       >  1:4654350/1‑93 (MQ=255)

                                                                   ttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATc                      >  2:2112337/1‑93 (MQ=255)

                                                                   ttATGCATAAGCCCTATCTCTGGCAACCATCTTCCATTGACCACATCGCTAGAATTCCCCTTCATAGCACGATGCCTTTCACTTATCGGCATc                      >  2:5045137/1‑93 (MQ=255)

                                                                    tATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCg                     >  2:4210297/1‑93 (MQ=255)

                                                                     aTGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATATAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGt                    >  1:6983164/1‑93 (MQ=255)

                                                                     aTGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGt                    >  1:4916030/1‑93 (MQ=255)

                                                                      tGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTg                   >  2:117885/1‑93 (MQ=255)

                                                                      tGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTg                   <  2:3209693/93‑1 (MQ=255)

                                                                        cATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCt                 >  2:5897634/1‑93 (MQ=255)

                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  1:5621171/1‑93 (MQ=255)

                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  2:2699009/1‑93 (MQ=255)

                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  1:1786528/1‑93 (MQ=255)

                                                                            aGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCca             >  2:4833277/1‑93 (MQ=255)

                                                                             gCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcac            >  1:6535452/1‑93 (MQ=255)

                                                                              cccTTTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcaca           <  1:6779727/93‑1 (MQ=255)

                                                                              cccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcaca           >  1:4857376/1‑93 (MQ=255)

                                                                              cccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGCGCTCCcaca           <  1:3891126/93‑1 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATATCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  2:7128074/1‑93 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          <  2:3376901/93‑1 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  2:4108397/1‑93 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  1:845398/1‑93 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  1:950791/1‑93 (MQ=255)

                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         >  2:7733798/1‑93 (MQ=255)

                                                                                cTATCTCTGACAACCGTCTTCCATTGGCCACATCGATAGCATCCTCCTACATAGCCCGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         <  2:7029587/93‑1 (MQ=255)

                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        <  2:2453406/93‑1 (MQ=255)

                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  2:6131308/1‑93 (MQ=255)

                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  2:1360119/1‑93 (MQ=255)

                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt       >  1:4450578/1‑93 (MQ=255)

                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt       <  2:2359823/93‑1 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      <  2:2897937/93‑1 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  1:4595644/1‑93 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  1:7459124/1‑93 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  2:1265404/1‑93 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  2:1933551/1‑93 (MQ=255)

                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     <  1:5832976/93‑1 (MQ=255)

                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     >  1:1922158/1‑93 (MQ=255)

                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCTGCATCGTGCTCCCACACGTTCCg    >  1:3524905/1‑93 (MQ=255)

                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    >  2:6351526/1‑93 (MQ=255)

                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    <  2:4444960/93‑1 (MQ=255)

                                                                                       tGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc  >  1:4258878/1‑93 (MQ=255)

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`