BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,496	T→C	G189G (GGT→GGC)	yeiG →	putative esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,496	0	T	C	98.4%	769.8 / ‑1.6	250	G189G (GGT→GGC)	yeiG	putative esterase
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (129/117); total (131/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

GGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGATCA  >  NC_000913/2242406‑2242585

gggTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGa                                                                                         <  2:4968592/93‑1 (MQ=255)

gggTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGa                                                                                         <  2:503325/93‑1 (MQ=255)

    aaaTACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGAc                                                                                     >  1:6666508/1‑93 (MQ=255)

         cacCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACaaaaa                                                                                <  2:3580830/93‑1 (MQ=255)

         cacCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACaaaaa                                                                                <  2:1496408/93‑1 (MQ=255)

         cacCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACaaaaa                                                                                <  2:4588938/93‑1 (MQ=255)

         cacCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACaaaaa                                                                                <  1:690587/93‑1 (MQ=255)

         cacCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACaaaaa                                                                                >  2:2757543/1‑93 (MQ=255)

           ccAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATg                                                                              >  1:4963409/1‑93 (MQ=255)

            cAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGc                                                                             >  1:4216608/1‑93 (MQ=255)

             aGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCa                                                                            <  1:4031502/93‑1 (MQ=255)

              gCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCAt                                                                           >  2:4043138/1‑93 (MQ=255)

               cGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATg                                                                          <  1:5024735/93‑1 (MQ=255)

                gTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATgg                                                                         >  1:2912353/1‑93 (MQ=255)

                gTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATgg                                                                         <  1:1429212/93‑1 (MQ=255)

                gTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATgg                                                                         >  2:6416137/1‑93 (MQ=255)

                 tttCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGc                                                                        >  2:6981099/1‑93 (MQ=255)

                   tCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTg                                                                      <  2:13759/93‑1 (MQ=255)

                      gCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACACAAATGCATGGCTGGaa                                                                   >  1:3365707/1‑93 (MQ=255)

                      gCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGaa                                                                   <  1:2234933/93‑1 (MQ=255)

                       ccTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAAt                                                                  <  1:6576836/93‑1 (MQ=255)

                        cTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATg                                                                 <  1:1078201/93‑1 (MQ=255)

                         tttGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATgg                                                                >  1:5652853/1‑93 (MQ=255)

                          ttGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATggg                                                               <  2:4783058/93‑1 (MQ=255)

                             cgcCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACa                                                            >  2:4626620/1‑93 (MQ=255)

                              gcCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAg                                                           <  2:4527463/93‑1 (MQ=255)

                               ccAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACCAAAATGCATGGCTGGAATGGGACCGt                                                          >  1:3538563/1‑93 (MQ=255)

                                cAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGtt                                                         <  2:2473983/93‑1 (MQ=255)

                                cAATTGTGAATCCATGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGtt                                                         >  1:2417923/1‑93 (MQ=255)

                                 aaTTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTg                                                        >  1:6962555/1‑93 (MQ=255)

                                 aaTTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTg                                                        >  1:2274878/1‑93 (MQ=255)

                                 aaTTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTg                                                        >  1:4532954/1‑93 (MQ=255)

                                     gtgAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGTTGGAATGGGACAGTTGCGCa                                                    >  1:18737/1‑93 (MQ=255)

                                      tgAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCAc                                                   >  1:5917024/1‑93 (MQ=255)

                                      tgAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCAc                                                   <  1:7631187/93‑1 (MQ=255)

                                      tgAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCAc                                                   <  2:4398960/93‑1 (MQ=255)

                                      tgAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCAc                                                   <  2:335183/93‑1 (MQ=255)

                                      tgAATCCGTGCAGCGTCCCGTGGCGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCAc                                                   <  2:4302617/93‑1 (MQ=255)

                                       gAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACt                                                  <  2:6677589/93‑1 (MQ=255)

                                       gAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACt                                                  <  2:3397403/93‑1 (MQ=255)

                                        aaTCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTg                                                 <  2:6105232/93‑1 (MQ=255)

                                         aTCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGa                                                <  2:3220206/93‑1 (MQ=255)

                                           ccGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATg                                              <  2:7292283/93‑1 (MQ=255)

                                             gTGCGGCGTCCGGTGGGAAATAAAAGCTTTTAGCAGTTATTTAGGCGAGGAAAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTa                                            <  1:3431017/93‑1 (MQ=255)

                                             gTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTa                                            <  2:3074497/93‑1 (MQ=255)

                                             gTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTa                                            >  1:1593043/1‑93 (MQ=255)

                                             gTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTa                                            <  2:3289483/93‑1 (MQ=255)

                                             gTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTa                                            >  2:7707950/1‑93 (MQ=255)

                                              tGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTAt                                           >  1:491519/1‑93 (MQ=255)

                                              tGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTAt                                           <  2:7245611/93‑1 (MQ=255)

                                              tGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTAt                                           >  2:5609329/1‑93 (MQ=255)

                                               gCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATg                                          <  1:6146318/93‑1 (MQ=255)

                                                cAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGc                                         <  1:722213/93‑1 (MQ=255)

                                                 aGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGcc                                        >  1:3322542/1‑93 (MQ=255)

                                                  gCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCa                                       <  2:1041411/93‑1 (MQ=255)

                                                  gCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCa                                       >  2:4929136/1‑93 (MQ=255)

                                                   cGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAg                                      >  1:120472/1‑93 (MQ=255)

                                                   cGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAg                                      <  2:5921543/93‑1 (MQ=255)

                                                    gTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAga                                     >  1:5777152/1‑92 (MQ=255)

                                                     tCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTa                                    >  1:6428238/1‑93 (MQ=255)

                                                     tCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTa                                    >  1:5612292/1‑93 (MQ=255)

                                                      cccGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTaa                                   <  2:2289427/93‑1 (MQ=255)

                                                      cccGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTaa                                   <  1:4644385/93‑1 (MQ=255)

                                                         gTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAAcgc                                <  2:6843092/93‑1 (MQ=255)

                                                          tGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAAcgcg                               >  1:769807/1‑93 (MQ=255)

                                                          tGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAAcgcg                               <  1:7261248/93‑1 (MQ=255)

                                                          tGGGGAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAAcgcg                               >  1:2035588/1‑93 (MQ=255)

                                                           ggggAATCAAAGCGTTTAGCAGCTATTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACgcgc                              <  1:3931729/93‑1 (MQ=255)

                                                            gggAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCa                             <  2:2739598/93‑1 (MQ=255)

                                                            gggAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCa                             <  1:2054764/93‑1 (MQ=255)

                                                            gggAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAAAGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCa                             <  1:1534249/93‑1 (MQ=255)

                                                              gAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAgg                           >  2:7877922/1‑93 (MQ=255)

                                                              gAATCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAgg                           >  1:2619196/1‑93 (MQ=255)

                                                               aaTCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGa                          >  2:1933384/1‑93 (MQ=255)

                                                               aaTCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGa                          >  2:4848490/1‑93 (MQ=255)

                                                               aaTCAAAGCGTTTAGCAACTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGa                          <  1:7029256/93‑1 (MQ=255)

                                                                aTCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGAt                         <  2:1795548/93‑1 (MQ=255)

                                                                 tCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATg                        >  2:3139749/1‑93 (MQ=255)

                                                                 tCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATg                        >  1:6645385/1‑93 (MQ=255)

                                                                 tCAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATg                        >  1:7895238/1‑93 (MQ=255)

                                                                 acAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATg                        <  2:6264025/92‑1 (MQ=255)

                                                                  cAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGc                       >  1:7748405/1‑93 (MQ=255)

                                                                  cAAAGCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGc                       <  2:5210789/93‑1 (MQ=255)

                                                                      gCGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATc                   >  2:7554128/1‑93 (MQ=255)

                                                                       cGTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATcc                  <  1:7515508/93‑1 (MQ=255)

                                                                        gTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATccc                 >  1:991777/1‑93 (MQ=255)

                                                                        gTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATccc                 >  1:3687064/1‑93 (MQ=255)

                                                                        gTTTAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATccc                 <  1:4443466/93‑1 (MQ=255)

                                                                           tAGCAGCTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGAc              <  2:5273535/93‑1 (MQ=255)

                                                                             gcagcTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGc            >  2:6189848/1‑93 (MQ=255)

                                                                               agcTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCtt          >  2:3636786/1‑93 (MQ=255)

                                                                                gcTATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTa         <  1:5278660/93‑1 (MQ=255)

                                                                                  tATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATc       >  1:5523609/1‑93 (MQ=255)

                                                                                  tATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATc       <  1:3849085/93‑1 (MQ=255)

                                                                                  tATTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATc       >  1:3584093/1‑93 (MQ=255)

                                                                                   aTTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGCTGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCg      >  2:1331405/1‑93 (MQ=255)

                                                                                   aTTTAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATACCAGTAACGCGCAGGATGCGATCCCGACGCTTATCg      <  2:3004460/93‑1 (MQ=255)

                                                                                    tttAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGa     >  2:2106009/1‑93 (MQ=255)

                                                                                    tttAGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGa     <  1:869796/93‑1 (MQ=255)

                                                                                       aGGCGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGATCa  <  1:7106389/93‑1 (MQ=255)

GGGTAAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTATTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCCCGACGCTTATCGATCA  >  NC_000913/2242406‑2242585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`