BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,918	A→G	T624T (ACT→ACC)	cirA ←	CirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,918	0	A	G	99.2%	819.3 / ‑4.0	258	T624T (ACT→ACC)	cirA	CirA
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (142/113); total (144/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 265 total aligned reads displayed.

ACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATA  >  NC_000913/2242828‑2243007

acgacgTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGt                                                                                         <  1:5542730/93‑1 (MQ=255)

  gacgTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCa                                                                                       >  2:650284/1‑93 (MQ=255)

    cgtccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCAcc                                                                                     >  2:7546302/1‑93 (MQ=255)

    cgtccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCAcc                                                                                     <  1:191386/93‑1 (MQ=255)

    cgtccgtcCTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCAcc                                                                                     <  2:3989937/93‑1 (MQ=255)

     gtccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCt                                                                                    >  2:166074/1‑93 (MQ=255)

     gtccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCt                                                                                    <  2:6672171/93‑1 (MQ=255)

     gtccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCt                                                                                    <  2:5382970/93‑1 (MQ=255)

     gtccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCt                                                                                    >  1:5978422/1‑93 (MQ=255)

      tccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTg                                                                                   >  2:2736793/1‑93 (MQ=255)

       ccgtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGc                                                                                  <  1:155345/93‑1 (MQ=255)

         gtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCa                                                                                <  2:7692435/93‑1 (MQ=255)

         gtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCa                                                                                <  1:1350154/93‑1 (MQ=255)

         gtcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCa                                                                                >  2:626281/1‑93 (MQ=255)

         gtcTTCGTGATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCa                                                                                <  2:7634117/93‑1 (MQ=255)

          tcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAg                                                                               >  2:1851128/1‑93 (MQ=255)

          tcTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAg                                                                               >  1:526111/1‑93 (MQ=255)

           cTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAgg                                                                              >  2:3360401/1‑93 (MQ=255)

           cTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAgg                                                                              >  2:7295237/1‑93 (MQ=255)

            ttCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGc                                                                             >  2:7576918/1‑93 (MQ=255)

            ttCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCAACTGCCAGGc                                                                             >  2:1794086/1‑93 (MQ=255)

             tCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGcc                                                                            <  1:3430781/93‑1 (MQ=255)

               gTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCcgc                                                                          >  1:107750/1‑93 (MQ=255)

                 tataGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCgcgc                                                                        >  1:6914395/1‑93 (MQ=255)

                  ataGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCATGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGcc                                                                       >  1:1770525/1‑93 (MQ=255)

                   taGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCg                                                                      <  2:4014281/93‑1 (MQ=255)

                       tGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTTGTCACCTTCCAGGCCGCGCCGGTAt                                                                  >  2:199044/1‑93 (MQ=255)

                       tGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTAt                                                                  <  2:79241/93‑1 (MQ=255)

                        gTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTAtt                                                                 >  1:3853693/1‑93 (MQ=255)

                         tAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTc                                                                <  2:7813078/93‑1 (MQ=255)

                          aGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTcc                                                               <  1:2330406/93‑1 (MQ=255)

                           gtcgtcACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCa                                                              <  2:4637949/93‑1 (MQ=255)

                           gtcgtcACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCa                                                              >  2:721238/1‑93 (MQ=255)

                             cgtcACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGa                                                            >  2:5910877/1‑93 (MQ=255)

                               tcACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATg                                                          >  1:4558487/1‑93 (MQ=255)

                               tcACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATg                                                          <  1:1736718/93‑1 (MQ=255)

                                cACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATgg                                                         >  1:7062189/1‑93 (MQ=255)

                                  cGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGta                                                       <  2:353457/93‑1 (MQ=255)

                                  cGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGta                                                       <  1:3135893/93‑1 (MQ=255)

                                  cGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGta                                                       <  2:1399345/93‑1 (MQ=255)

                                    aCTGAGATCTTTGTCGCGAAGGTTCAGGACGCCTGCGCGCAGTTGGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGtata                                                     <  1:7401183/93‑1 (MQ=255)

                                    aCTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGtata                                                     <  1:6755615/93‑1 (MQ=255)

                                    aCTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGtata                                                     >  1:4277903/1‑93 (MQ=255)

                                     cTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATaa                                                    >  1:419424/1‑93 (MQ=255)

                                     cTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATaa                                                    >  2:256881/1‑93 (MQ=255)

                                      tGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAAc                                                   <  1:2875590/93‑1 (MQ=255)

                                      tGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAAc                                                   >  1:3007349/1‑93 (MQ=255)

                                      tGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATCCCTGTTGGTATAAc                                                   >  1:2429640/1‑93 (MQ=255)

                                       gagaTCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAAcc                                                  >  1:1127637/1‑93 (MQ=255)

                                       gagaTCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAAcc                                                  <  1:5691970/93‑1 (MQ=255)

                                       gagaTCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAAcc                                                  >  1:6264265/1‑93 (MQ=255)

                                         gaTCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGc                                                >  1:6175028/1‑93 (MQ=255)

                                          aTCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGCATTCCAGATGGTATAACCGcc                                               >  2:6814989/1‑93 (MQ=255)

                                           tCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGccc                                              >  2:7456845/1‑93 (MQ=255)

                                            ccTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCg                                             >  1:5086292/1‑93 (MQ=255)

                                              ttGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGgt                                           >  2:4790408/1‑93 (MQ=255)

                                                  cGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGttt                                       <  1:4699099/93‑1 (MQ=255)

                                                    ccAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTa                                     >  1:374990/1‑93 (MQ=255)

                                                    ccAAGGTGCAGCCCGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTa                                     <  1:3962969/93‑1 (MQ=255)

                                                     cAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAg                                    >  1:3431857/1‑93 (MQ=255)

                                                     cAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAg                                    >  1:6522438/1‑93 (MQ=255)

                                                     cAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAg                                    >  1:4381390/1‑93 (MQ=255)

                                                        ggTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCg                                 >  1:2808719/1‑93 (MQ=255)

                                                        ggTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCg                                 <  2:2580925/93‑1 (MQ=255)

                                                          ttCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTc                               <  1:5905548/93‑1 (MQ=255)

                                                          ttCAGCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTc                               <  2:320452/93‑1 (MQ=255)

                                                              gCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCgcgc                           <  1:5543322/93‑1 (MQ=255)

                                                              gCACGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCgcgc                           <  2:4190552/93‑1 (MQ=255)

                                                              gCACGCCCGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGGCCGGTGTTTTAACCGTCgcgc                           >  2:3741593/1‑93 (MQ=255)

                                                                aCGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTg                         >  2:569088/1‑93 (MQ=255)

                                                                aCGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTg                         >  2:3882738/1‑93 (MQ=255)

                                                                 cGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGt                        <  2:2102716/93‑1 (MQ=255)

                                                                 cGCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGt                        >  1:4947588/1‑93 (MQ=255)

                                                                  gCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTc                       <  2:3016513/93‑1 (MQ=255)

                                                                  gCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTc                       <  2:6985402/93‑1 (MQ=255)

                                                                  gCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTc                       >  2:6525919/1‑93 (MQ=255)

                                                                  gCCTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTc                       <  1:5373621/93‑1 (MQ=255)

                                                                   ccTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCg                      >  2:1214173/1‑93 (MQ=255)

                                                                   ccTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCg                      <  1:803721/93‑1 (MQ=255)

                                                                    cTGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCgg                     <  1:6454179/93‑1 (MQ=255)

                                                                     tGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGgc                    <  1:6389667/93‑1 (MQ=255)

                                                                     tGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGgc                    >  1:4855466/1‑93 (MQ=255)

                                                                     tGCGCGCAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGgc                    >  1:7481520/1‑93 (MQ=255)

                                                                      gcgcgcAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGgcg                   <  1:3302163/93‑1 (MQ=255)

                                                                       cgcgcAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGgcgc                  <  1:7794232/93‑1 (MQ=255)

                                                                        gcgcAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGcgcg                 >  2:561748/1‑93 (MQ=255)

                                                                         cgcAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGt                >  1:4874086/1‑93 (MQ=255)

                                                                          gcAGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGtt               <  2:8883/93‑1 (MQ=255)

                                                                            aGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTc             >  2:2327708/1‑93 (MQ=255)

                                                                            aGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTc             <  1:5801131/93‑1 (MQ=255)

                                                                            aGTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTc             >  1:985355/1‑93 (MQ=255)

                                                                             gTTTGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCt            >  1:926740/1‑93 (MQ=255)

                                                                              tttGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTg           >  2:602671/1‑93 (MQ=255)

                                                                              tttGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTg           >  1:564276/1‑93 (MQ=255)

                                                                                tGACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGcc         <  1:215688/93‑1 (MQ=255)

                                                                                 gACGTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGccc        <  2:1367532/93‑1 (MQ=255)

                                                                                    gTCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCACTGTCGGCGCGTTTCTGCCCGGt     >  1:1260034/1‑93 (MQ=255)

                                                                                     tCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGta    >  1:51210/1‑93 (MQ=255)

                                                                                     tCTTTGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGta    >  1:2849952/1‑93 (MQ=255)

                                                                                       tttGGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGtata  >  1:4487208/1‑93 (MQ=255)

ACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGCGCAGTTTGACGTCTTTAGTCACCTGCCAGGCCGCGCCGGTATTCCAGATGGTATAACCGCCCGGTGTTTTAGCCGTCGCGCTGTCGGCGCGTTTCTGCCCGGTATA  >  NC_000913/2242828‑2243007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`