BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,919	C→T	R156H (CGC→CAC)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,919	0	C	T	100.0%	855.4 / NA	242	R156H (CGC→CAC)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (112/130); total (112/130)

Only 100 of 248 total aligned reads displayed.

GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259829‑2260008

ggTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGa                                                                                         <  1:2019513/93‑1 (MQ=255)

 gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAg                                                                                        >  1:4330390/1‑93 (MQ=255)

      aCCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATc                                                                                   >  2:1565303/1‑93 (MQ=255)

      aCCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATc                                                                                   <  2:1920667/93‑1 (MQ=255)

       ccAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATcc                                                                                  <  1:2285097/93‑1 (MQ=255)

        cAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCa                                                                                 <  1:7722714/93‑1 (MQ=255)

          gCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGt                                                                               >  1:5866727/1‑93 (MQ=255)

           ccACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTc                                                                              <  2:5976772/93‑1 (MQ=255)

            cACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCg                                                                             >  2:139817/1‑93 (MQ=255)

             aCGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCgg                                                                            <  2:2375145/93‑1 (MQ=255)

              cGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGt                                                                           >  1:4066948/1‑93 (MQ=255)

                 tGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGaa                                                                        <  1:5212947/93‑1 (MQ=255)

                     gCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCt                                                                    >  1:1003511/1‑93 (MQ=255)

                     gCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCt                                                                    >  2:5630063/1‑93 (MQ=255)

                      cTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCtt                                                                   >  2:3820633/1‑93 (MQ=255)

                       tttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  1:1103948/93‑1 (MQ=255)

                       tttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  2:6117923/93‑1 (MQ=255)

                       tttCAAACCTGCTACTAACGCTACACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTc                                                                  <  1:3872113/93‑1 (MQ=255)

                        ttCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTcc                                                                 <  2:5096831/93‑1 (MQ=255)

                         tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  2:247842/93‑1 (MQ=255)

                         tCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCg                                                                <  2:7065535/93‑1 (MQ=255)

                          cAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCgg                                                               <  1:7313593/93‑1 (MQ=255)

                            aaCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGc                                                             <  2:3741523/93‑1 (MQ=255)

                            aaCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGc                                                             >  1:85646/1‑93 (MQ=255)

                             aCCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCt                                                            <  1:2976413/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  1:2060545/93‑1 (MQ=255)

                               cTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGa                                                          <  2:7722272/93‑1 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGACCGCTTCCGGGCTGAc                                                         >  1:408522/1‑93 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         <  2:1452776/93‑1 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         >  1:6786875/1‑93 (MQ=255)

                                tGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGAc                                                         >  1:1164610/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  2:7649328/1‑93 (MQ=255)

                                   tactaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                      >  1:2020917/1‑93 (MQ=255)

                                     ctaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTg                                                    <  1:6402652/93‑1 (MQ=255)

                                     ctaACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTg                                                    <  1:7897284/93‑1 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   <  1:4166238/93‑1 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:1681349/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:4664583/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                                                   >  1:3240662/1‑93 (MQ=255)

                                      taACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGATGACGCCTGa                                                   >  2:4816859/1‑93 (MQ=255)

                                        aCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACg                                                 <  1:2426838/93‑1 (MQ=255)

                                        aCGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTAACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACg                                                 <  2:541379/93‑1 (MQ=255)

                                         cggttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACgg                                                <  2:5963366/90‑1 (MQ=255)

                                            ttCACGGCTACTATCAAAGATAATTCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  2:2761496/93‑1 (MQ=255)

                                            ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  1:1778367/93‑1 (MQ=255)

                                            ttCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTaa                                             <  2:7336706/93‑1 (MQ=255)

                                             tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            <  2:7576012/93‑1 (MQ=255)

                                             tCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                                            <  1:3831422/93‑1 (MQ=255)

                                                cGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCtt                                         >  1:1523078/1‑93 (MQ=255)

                                                 ggCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCGTCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTc                                        <  2:1840781/93‑1 (MQ=255)

                                                 ggCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTc                                        <  2:1288205/93‑1 (MQ=255)

                                                    tactaTCAAAGATAATACAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGc                                     >  2:5221123/1‑93 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  >  2:648291/1‑93 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  <  1:1925946/93‑1 (MQ=255)

                                                       taTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGa                                  >  1:6345389/1‑93 (MQ=255)

                                                        aTCAAAGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGac                                 <  2:3151265/93‑1 (MQ=255)

                                                          cAAAGATAATGCAAGGACCCTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGacac                               <  1:6565152/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  2:4363782/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:6086802/1‑93 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              <  2:1823878/93‑1 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  2:3271827/1‑93 (MQ=255)

                                                           aaaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca                              >  1:1425115/1‑93 (MQ=255)

                                                            aaGATAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcacaa                             >  1:3182893/1‑92 (MQ=255)

                                                               aTAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                          <  2:7894112/93‑1 (MQ=255)

                                                               aTAATGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                          >  1:3447656/1‑93 (MQ=255)

                                                                 aaTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCat                        >  2:7219914/1‑93 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       >  1:940178/1‑93 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                       >  2:5782589/1‑93 (MQ=255)

                                                                  aTGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGAGAGCTTCCGCTGACACAGACCata                       >  2:4308161/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      >  2:6866202/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      >  2:1030281/1‑93 (MQ=255)

                                                                   tGCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCatat                      <  2:801096/93‑1 (MQ=255)

                                                                    gCAAGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATc                     >  2:6566987/1‑93 (MQ=255)

                                                                    gCAAGCCCACTGCCTCCGCAGGTGAGTCATCCAGTCGGTGATCGCTTCCGGCCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATc                     <  2:3413753/93‑1 (MQ=255)

                                                                       aGGACACTGACTCCGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  <  2:2090910/93‑1 (MQ=255)

                                                                       aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  >  1:4962011/1‑93 (MQ=255)

                                                                       aGGACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa                  >  1:709538/1‑93 (MQ=255)

                                                                        ggACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAAc                 <  1:4774500/93‑1 (MQ=255)

                                                                         gACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACt                >  1:5709945/1‑93 (MQ=255)

                                                                         gACACTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACt                <  2:4714745/93‑1 (MQ=255)

                                                                          acacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTg               >  1:5264563/1‑93 (MQ=255)

                                                                          acacTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTg               >  1:3801440/1‑93 (MQ=255)

                                                                            acTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAc             <  1:7468661/93‑1 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            >  1:3035719/1‑93 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            >  2:2666804/1‑93 (MQ=255)

                                                                             cTGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGAcc            >  1:7679108/1‑93 (MQ=255)

                                                                              tGACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCg           >  1:741316/1‑93 (MQ=255)

                                                                              tGACTACGCAGGTCAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTACGCTGACACAGACCATATCGAACTGACCg           >  1:4540690/1‑93 (MQ=255)

                                                                               gccTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          <  2:2248987/91‑1 (MQ=255)

                                                                               gACTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGa          >  1:2633622/1‑93 (MQ=255)

                                                                                 cTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGc        >  1:6447250/1‑93 (MQ=255)

                                                                                 cTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGc        >  1:1326958/1‑93 (MQ=255)

                                                                                 cTACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGc        >  2:215250/1‑93 (MQ=255)

                                                                                  tACGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGcc       <  1:3669783/93‑1 (MQ=255)

                                                                                   gcGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCa      <  2:3039490/92‑1 (MQ=255)

                                                                                   aCGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCa      <  2:6739348/93‑1 (MQ=255)

                                                                                    cGCAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAg     <  1:5464465/93‑1 (MQ=255)

                                                                                      cAGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCt   >  1:533025/1‑93 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  <  2:746691/93‑1 (MQ=255)

                                                                                       aGGTGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTc  >  2:1731652/1‑93 (MQ=255)

GGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAGCTC  >  NC_000913/2259829‑2260008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`