BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,885	A→G	S13G (AGC→GGC)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,885	0	A	G	99.5%	624.6 / ‑5.0	202	S13G (AGC→GGC)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (101/99); total (102/100)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

Only 100 of 223 total aligned reads displayed.

TTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTC  >  NC_000913/2265795‑2265974

 tttttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGgcggcg                                                                                        >  2:6706834/1‑93 (MQ=255)

  ttttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTcggcggcgg                                                                                       <  1:904336/93‑1 (MQ=255)

    ttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGa                                                                                     <  1:6463809/93‑1 (MQ=255)

     tAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCgggga                                                                                    >  2:3188657/1‑91 (MQ=255)

         cAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGACAAcc                                                                                >  2:2527465/1‑93 (MQ=255)

         cAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAAcc                                                                                >  2:3040743/1‑93 (MQ=255)

           ggATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGGAAACCAc                                                                              >  2:1630536/1‑93 (MQ=255)

            aaTTACCGCTCAGTTACTTTACAAGAGTATTGCGGAGCCGTTGCGACCAGGACCACCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACg                                                                             <  2:7099020/92‑1 (MQ=255)

             aTTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGt                                                                            <  2:2979518/93‑1 (MQ=255)

                aaCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGa                                                                         <  1:7692382/93‑1 (MQ=255)

                 aCGCTCAGTTACCTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCTCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAt                                                                        <  1:1046597/93‑1 (MQ=255)

                 aCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAt                                                                        <  1:4533605/93‑1 (MQ=255)

                 aCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAt                                                                        <  1:3608474/93‑1 (MQ=255)

                  cGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAtt                                                                       >  1:7531161/1‑93 (MQ=255)

                  cGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAtt                                                                       <  2:1124410/93‑1 (MQ=255)

                  cGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAtt                                                                       >  2:1418710/1‑93 (MQ=255)

                  cGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAtt                                                                       >  2:3378103/1‑93 (MQ=255)

                  cGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAtt                                                                       >  2:5474269/1‑93 (MQ=255)

                   gCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttc                                                                      <  2:867836/93‑1 (MQ=255)

                   gCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttc                                                                      >  2:4554336/1‑93 (MQ=255)

                    cTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttct                                                                     >  1:4332025/1‑93 (MQ=255)

                     tCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttctt                                                                    >  1:7835126/1‑93 (MQ=255)

                     tCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttctt                                                                    >  1:5474383/1‑93 (MQ=255)

                       atttAACTAACCAGAGTTTGGCGGAGCCGTTGTGCCCAGGCCCACCCTCATCCCCGTTTTTCGCGGCGGGGGAAAACCCACGTCGATTCTTCa                                                                  <  2:7005292/91‑1 (MQ=255)

                       aGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCa                                                                  <  2:2743811/93‑1 (MQ=255)

                        gTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCAt                                                                 <  1:3388181/93‑1 (MQ=255)

                         ttAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATc                                                                <  2:3890194/93‑1 (MQ=255)

                         ttAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATc                                                                >  1:2100499/1‑93 (MQ=255)

                          tAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCt                                                               <  1:5054665/93‑1 (MQ=255)

                              ttACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTa                                                           <  1:791401/93‑1 (MQ=255)

                                aCCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGGGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGc                                                         <  1:186446/93‑1 (MQ=255)

                                aCCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACTACGTCGATTCTTCATCTGTTAGc                                                         >  2:4308332/1‑93 (MQ=255)

                                 ccAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGcc                                                        >  2:2801068/1‑93 (MQ=255)

                                 ccAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGcc                                                        >  2:4010887/1‑93 (MQ=255)

                                  cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGccc                                                       >  1:2866877/1‑93 (MQ=255)

                                  cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGccc                                                       >  1:3855789/1‑93 (MQ=255)

                                  cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGccc                                                       >  1:6044591/1‑93 (MQ=255)

                                  cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGcac                                                       >  2:3838471/1‑91 (MQ=255)

                                  cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAAACACGTCGATTCTTCATCTGTTAGccc                                                       >  2:5669119/1‑93 (MQ=255)

                                   agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCa                                                      >  2:1253024/1‑93 (MQ=255)

                                    aagTTTTGGGGAGCCGTTGTGCCCAGGACCACCCGCATCACCGGTTTTCTCGGCGGCGGAAAAACCACGTCGATTCTTCATCTGTTAGCCCAt                                                     <  1:1436098/92‑1 (MQ=255)

                                     agTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCCCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATa                                                    <  1:6362830/93‑1 (MQ=255)

                                     agTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATa                                                    >  1:4722505/1‑93 (MQ=255)

                                       tATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATaaa                                                  >  2:7240894/1‑93 (MQ=255)

                                       tATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATaaa                                                  >  2:6628832/1‑93 (MQ=255)

                                        aTTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAg                                                 <  1:3923964/93‑1 (MQ=255)

                                        aTTGAGGACCCGCTGTGACCAGGCCCAACCTGATCTCCGGTTTGCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAg                                                 <  2:3506193/93‑1 (MQ=255)

                                         ttGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGa                                                >  1:3918954/1‑93 (MQ=255)

                                           gCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATc                                              <  1:6641203/93‑1 (MQ=255)

                                           gCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATc                                              <  2:6677551/93‑1 (MQ=255)

                                            cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATcc                                             <  1:172648/93‑1 (MQ=255)

                                            cGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATcc                                             >  2:5180905/1‑93 (MQ=255)

                                              gAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCa                                           >  2:6415667/1‑93 (MQ=255)

                                              gAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCa                                           >  2:5694522/1‑93 (MQ=255)

                                               aGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCaa                                          <  1:7390832/93‑1 (MQ=255)

                                                gCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAAc                                         >  2:6179545/1‑93 (MQ=255)

                                                   gTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCGCAACGaa                                      >  1:2973269/1‑93 (MQ=255)

                                                   gTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaa                                      >  2:1472694/1‑93 (MQ=255)

                                                   gTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaa                                      <  1:3464067/93‑1 (MQ=255)

                                                   gTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaa                                      >  2:7603879/1‑93 (MQ=255)

                                                    ttGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCCACGaaa                                     >  2:2243296/1‑93 (MQ=255)

                                                    ttGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaa                                     >  2:2843828/1‑93 (MQ=255)

                                                    ttGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaa                                     >  2:6947447/1‑93 (MQ=255)

                                                      gtgGCCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaaa                                   <  2:5939529/93‑1 (MQ=255)

                                                        gACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATg                                 <  2:5508676/93‑1 (MQ=255)

                                                        gACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATg                                 >  2:4154604/1‑93 (MQ=255)

                                                         aCCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATgg                                >  1:4196916/1‑93 (MQ=255)

                                                         aCCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATgg                                <  1:2310440/93‑1 (MQ=255)

                                                           cAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGc                              >  1:2354055/1‑93 (MQ=255)

                                                           cAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGc                              >  2:1699607/1‑93 (MQ=255)

                                                           cAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGc                              >  2:1191202/1‑93 (MQ=255)

                                                             ggACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCgg                            <  2:5288644/93‑1 (MQ=255)

                                                             ggACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCgg                            <  1:3996937/93‑1 (MQ=255)

                                                              gACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGt                           <  1:1233329/93‑1 (MQ=255)

                                                                ccACCCTCACCACCGTTTTTCTCGGCGGCGGAAAACCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTAc                         <  2:6938553/93‑1 (MQ=255)

                                                                 cAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACt                        <  2:2041538/93‑1 (MQ=255)

                                                                  aaCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTg                       <  1:2932105/93‑1 (MQ=255)

                                                                  aaCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTg                       >  1:6167195/1‑93 (MQ=255)

                                                                    ccTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGt                     <  2:4641943/93‑1 (MQ=255)

                                                                    ccTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGt                     <  2:7214384/93‑1 (MQ=255)

                                                                      tcatcaCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTa                   >  2:4409330/1‑93 (MQ=255)

                                                                      tcatcaCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTa                   >  1:1272555/1‑93 (MQ=255)

                                                                       catcaCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTaa                  >  1:5712289/1‑93 (MQ=255)

                                                                         tcaCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATg                <  2:1768178/93‑1 (MQ=255)

                                                                          caCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGa               <  1:513739/93‑1 (MQ=255)

                                                                           aCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa              <  1:3054389/93‑1 (MQ=255)

                                                                           aCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa              >  2:7894276/1‑93 (MQ=255)

                                                                           aCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa              <  2:642009/93‑1 (MQ=255)

                                                                            cgggTTTCCGCGGCGGCGGAAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAAt             <  1:6935536/91‑1 (MQ=255)

                                                                              ggTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAAttt           <  1:5381852/93‑1 (MQ=255)

                                                                               gTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAACAATGGGCGGTACTGGTTAATGAATTTg          >  1:3407665/1‑93 (MQ=255)

                                                                                ttttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg         <  2:7422247/93‑1 (MQ=255)

                                                                                ttttCTCGGCCGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg         >  1:7187060/1‑93 (MQ=255)

                                                                                  ttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgggg       >  1:3240910/1‑93 (MQ=255)

                                                                                  ttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgggg       <  1:609187/93‑1 (MQ=255)

                                                                                   tctcGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGa      <  1:7299494/93‑1 (MQ=255)

                                                                                    ctcGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGaa     <  2:2349528/93‑1 (MQ=255)

                                                                                    ctcGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGaa     <  1:6715625/93‑1 (MQ=255)

                                                                                      cggcggcggGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGt   <  1:4973669/93‑1 (MQ=255)

                                                                                      cggcggcggGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGATACTGGTTAATGAATTTGGGGAAGt   <  2:4796860/93‑1 (MQ=255)

TTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTC  >  NC_000913/2265795‑2265974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`