BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,741	T→C	D76G (GAC→GGC)	yejK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,741	0	T	C	99.5%	657.2 / ‑5.4	214	D76G (GAC→GGC)	yejK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (131/82); total (131/83)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 239 total aligned reads displayed.

CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA  >  NC_000913/2281651‑2281829

ccaaaCAGCAATAGTGCACCGTAATCAATAGGATATTTCGCTAATTAGTCACGCAGGCGGCGGGTTGCCGCGCGGCTAAATGCCAGGAAGccc                                                                                        <  2:4399305/91‑1 (MQ=255)

cAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGccc                                                                                        <  1:433287/93‑1 (MQ=255)

  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctc                                                                                      >  2:6905987/1‑93 (MQ=255)

   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                     <  1:7600670/93‑1 (MQ=255)

   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGAGGCCGGCTGCCGCGCGGCTAAATGCCAGCAAGCCctct                                                                                     >  1:822668/1‑93 (MQ=255)

     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACTCAGGCGGCCTGTTGCCGCGCGGCCAAATGCCGGGCACGCctctcg                                                                                   >  1:5988095/1‑91 (MQ=255)

     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                   <  2:2254004/93‑1 (MQ=255)

     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                   <  1:995784/93‑1 (MQ=255)

      aGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGAGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCg                                                                                  >  2:7872968/1‑93 (MQ=255)

       gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                 >  1:4094346/1‑93 (MQ=255)

       gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                 >  1:2617040/1‑93 (MQ=255)

        cACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGcc                                                                                >  1:6967070/1‑93 (MQ=255)

         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCCTCGccc                                                                               >  2:7657555/1‑93 (MQ=255)

         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                               >  2:4014167/1‑93 (MQ=255)

         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                               <  2:5345433/93‑1 (MQ=255)

         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                               >  2:2685934/1‑93 (MQ=255)

            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                            <  1:3253224/93‑1 (MQ=255)

            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                            <  1:4711852/93‑1 (MQ=255)

             aTCCACCGTCAGCAAAAGGATATATCGCTAATTCGTCACGCAGGCTGACGGTTGCCGCGCGGCTAAATGCCAGGACGCCCTCTTCGCCCTGAc                                                                           >  2:3789463/1‑93 (MQ=255)

               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                         <  1:2512041/93‑1 (MQ=255)

               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                         <  1:1940248/93‑1 (MQ=255)

                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                        >  2:6689151/1‑93 (MQ=255)

                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                        >  1:391490/1‑93 (MQ=255)

                 aCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTg                                                                       >  1:2009434/1‑93 (MQ=255)

                 aCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTg                                                                       <  1:2299206/93‑1 (MQ=255)

                      cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                  >  1:2182632/1‑93 (MQ=255)

                       aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcg                                                                 >  2:4273818/1‑93 (MQ=255)

                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                               >  2:805880/1‑93 (MQ=255)

                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                               <  1:7844120/93‑1 (MQ=255)

                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                               <  2:4391928/93‑1 (MQ=255)

                          aaaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                              >  1:7753438/1‑93 (MQ=255)

                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                         <  1:492263/93‑1 (MQ=255)

                                atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                        <  2:3458894/93‑1 (MQ=255)

                                 tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                       >  2:6256906/1‑93 (MQ=255)

                                 tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                       >  1:6415998/1‑93 (MQ=255)

                                  atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                      >  2:3026792/1‑93 (MQ=255)

                                  atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                      >  2:721475/1‑93 (MQ=255)

                                   tttCGCCAATTCGTCACGCAGGCGGCCTGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcgc                                                     <  1:2738553/93‑1 (MQ=255)

                                      cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAg                                                  <  1:1209879/93‑1 (MQ=255)

                                      cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAg                                                  <  1:6547545/93‑1 (MQ=255)

                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                               >  1:6490680/1‑93 (MQ=255)

                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                               >  1:3128392/1‑93 (MQ=255)

                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                            >  2:7346122/1‑93 (MQ=255)

                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                            >  2:1571720/1‑93 (MQ=255)

                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                            <  1:412858/93‑1 (MQ=255)

                                              cGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                          <  1:732290/93‑1 (MQ=255)

                                               gTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctc                                         >  2:3373178/1‑93 (MQ=255)

                                                tCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctct                                        >  1:7560828/1‑93 (MQ=255)

                                                  ccGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                      <  1:5539890/92‑1 (MQ=255)

                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                      <  2:236475/93‑1 (MQ=255)

                                                    gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                    <  2:6378766/93‑1 (MQ=255)

                                                     cAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCt                                   >  1:6697184/1‑93 (MQ=255)

                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  1:5179597/1‑93 (MQ=255)

                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  1:3287649/1‑93 (MQ=255)

                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  2:7429805/1‑93 (MQ=255)

                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  2:2760252/1‑93 (MQ=255)

                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  2:1079972/1‑93 (MQ=255)

                                                         cggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCATCTGCGCCAGTTCACTCTCTTCGCTAAAc                               <  2:4795712/93‑1 (MQ=255)

                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             <  1:1746335/93‑1 (MQ=255)

                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             <  1:7427010/93‑1 (MQ=255)

                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             <  2:6051/93‑1 (MQ=255)

                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             >  1:3558008/1‑93 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           <  2:3875433/93‑1 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           <  2:5433478/93‑1 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           >  2:1279267/1‑93 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           >  1:3822957/1‑93 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           >  2:1788358/1‑93 (MQ=255)

                                                              ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                          <  1:5333071/93‑1 (MQ=255)

                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGCCGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         <  1:2995985/93‑1 (MQ=255)

                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         <  2:6207723/93‑1 (MQ=255)

                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         >  2:4800524/1‑93 (MQ=255)

                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         >  1:7177091/1‑93 (MQ=255)

                                                                ttGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGt                        >  2:1030706/1‑93 (MQ=255)

                                                                    cgcgcgGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGc                    <  2:3674678/93‑1 (MQ=255)

                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTTACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCTGCAAGCttt                 >  2:2340386/1‑93 (MQ=255)

                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCttt                 >  1:1579162/1‑93 (MQ=255)

                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCttt                 >  1:4440966/1‑93 (MQ=255)

                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCCCCCTGACCCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCttt                 <  1:2639268/93‑1 (MQ=255)

                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                <  2:5923351/93‑1 (MQ=255)

                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                >  1:397341/1‑93 (MQ=255)

                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                >  2:5505274/1‑93 (MQ=255)

                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                <  2:2141972/93‑1 (MQ=255)

                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt              <  2:1858127/93‑1 (MQ=255)

                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt              <  2:2509885/93‑1 (MQ=255)

                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt              <  2:6351841/93‑1 (MQ=255)

                                                                            tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt            >  2:1425140/1‑93 (MQ=255)

                                                                            tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt            >  1:571778/1‑93 (MQ=255)

                                                                              aaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg          >  2:3279633/1‑93 (MQ=255)

                                                                                   cAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCt     >  1:6853937/1‑93 (MQ=255)

                                                                                   cAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCt     >  1:6201773/1‑93 (MQ=255)

                                                                                   cAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCt     >  1:3667473/1‑93 (MQ=255)

                                                                                    aGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTTGCGCta    >  1:3595362/1‑93 (MQ=255)

                                                                                    aGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta    >  2:5768295/1‑93 (MQ=255)

                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat   >  2:5554222/1‑93 (MQ=255)

                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat   >  1:5275677/1‑93 (MQ=255)

                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat   >  1:2389116/1‑93 (MQ=255)

                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  <  2:4804510/93‑1 (MQ=255)

                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  >  2:2215913/1‑93 (MQ=255)

                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  >  2:5270986/1‑93 (MQ=255)

                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  <  2:294464/93‑1 (MQ=255)

CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA  >  NC_000913/2281651‑2281829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`