Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 232,218 A→G D98G (GAC→GGC)  yafE → putative biotin synthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913232,2180AG99.7% 929.4 / ‑5.0 300D98G (GAC→GGC) yafEputative biotin synthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (149/150);  total (149/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

Only 100 of 309 total aligned reads displayed.

GTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCA  >  NC_000913/232128‑232307
                                                                                          |                                                                                         
gTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCg                                                                                         >  1:3527660/1‑93 (MQ=255)
 ttATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGt                                                                                        >  1:3318106/1‑93 (MQ=255)
  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       >  2:5522464/1‑93 (MQ=255)
   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTATGCTGATTGTGATGGACGTaa                                                                                      >  1:4639238/1‑93 (MQ=255)
   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGGGTaa                                                                                      >  1:6055291/1‑93 (MQ=255)
   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTaa                                                                                      >  1:2458294/1‑93 (MQ=255)
    ttCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAAt                                                                                     <  2:5787783/93‑1 (MQ=255)
    ttCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAAt                                                                                     <  2:333940/93‑1 (MQ=255)
    ttCTGCCCATCACTGGCATGATGTTGGTCCAGCACTGCGAGAAGTAAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAAt                                                                                     <  1:169839/93‑1 (MQ=255)
        gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtct                                                                                 <  2:6854842/93‑1 (MQ=255)
         cccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtctc                                                                                <  1:869236/93‑1 (MQ=255)
          ccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTcc                                                                               <  1:4193017/93‑1 (MQ=255)
          ccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTcc                                                                               <  1:7343697/93‑1 (MQ=255)
           cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCg                                                                              <  1:5591426/93‑1 (MQ=255)
            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             >  1:1878027/1‑93 (MQ=255)
             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            >  2:3439169/1‑93 (MQ=255)
             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            >  1:2201949/1‑93 (MQ=255)
              cACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGt                                                                           >  2:484505/1‑93 (MQ=255)
               aCTGGCATGATGTTGGGGAAGCACTGCGAGAAGTGAACAGGATATTGAAAACTGCAGGTGGGATGATTATGATGGGCGTAAGGTGTCCGGGTc                                                                          >  2:502016/1‑93 (MQ=255)
                cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCa                                                                         >  2:1363483/1‑93 (MQ=255)
                cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCa                                                                         <  1:4235023/93‑1 (MQ=255)
                 tGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCAc                                                                        <  2:5456421/93‑1 (MQ=255)
                    cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCa                                                                     <  2:930968/93‑1 (MQ=255)
                    cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCa                                                                     <  1:2900061/93‑1 (MQ=255)
                     atgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAg                                                                    >  1:2970580/1‑93 (MQ=255)
                     atgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAg                                                                    <  1:1623449/93‑1 (MQ=255)
                     atgatgGTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGCCTCATTGTGATGGGCGTAATGTCTCCGGGTCACCCAg                                                                    <  1:4474227/93‑1 (MQ=255)
                      tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGt                                                                   <  1:6361677/93‑1 (MQ=255)
                      tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGt                                                                   >  1:3619287/1‑93 (MQ=255)
                       gatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTg                                                                  <  2:2342749/93‑1 (MQ=255)
                        atgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgc                                                                 <  2:2094907/93‑1 (MQ=255)
                        atgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgc                                                                 <  2:6195524/93‑1 (MQ=255)
                         tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgcg                                                                >  2:4598761/1‑93 (MQ=255)
                             ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGAc                                                            <  2:2789467/93‑1 (MQ=255)
                              gTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACa                                                           >  1:7936623/1‑93 (MQ=255)
                                gcagcaCTGCGAGAAGTGAATATGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCCCCCAGTGCGCGACATc                                                         <  2:5889751/93‑1 (MQ=255)
                                  agcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTg                                                       >  2:7611933/1‑93 (MQ=255)
                                  agcaCTGCGAAAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTg                                                       <  1:4427999/93‑1 (MQ=255)
                                     aCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                                    <  2:4961352/93‑1 (MQ=255)
                                       tGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                                  >  1:7871723/1‑93 (MQ=255)
                                       tGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                                  <  1:1031745/93‑1 (MQ=255)
                                       tGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                                  >  2:4545111/1‑93 (MQ=255)
                                       tGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                                  >  1:345103/1‑93 (MQ=255)
                                        gCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                                 <  2:3480707/93‑1 (MQ=255)
                                         cGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAg                                                <  1:4779354/93‑1 (MQ=255)
                                           agaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                                              >  2:3996348/1‑93 (MQ=255)
                                            gaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTGCAGACg                                             >  2:2645578/1‑93 (MQ=255)
                                            gaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACg                                             >  1:24897/1‑93 (MQ=255)
                                              aGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                                           <  2:1934189/93‑1 (MQ=255)
                                                tGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCACGACATCTGGTTACAGACGGTAg                                         >  2:5657171/1‑93 (MQ=255)
                                                 gAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                                        >  1:2044363/1‑93 (MQ=255)
                                                 gAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                                        <  2:6824184/93‑1 (MQ=255)
                                                 gAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                                        >  2:2403952/1‑93 (MQ=255)
                                                    tAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                                     >  2:1666022/1‑93 (MQ=255)
                                                    tAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                                     <  1:4335693/93‑1 (MQ=255)
                                                     aGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCa                                    <  2:7053374/93‑1 (MQ=255)
                                                     aGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCa                                    >  1:194773/1‑93 (MQ=255)
                                                      ggATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                                   <  2:6059696/93‑1 (MQ=255)
                                                      ggATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                                   <  1:1481636/93‑1 (MQ=255)
                                                      ggATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                                   >  2:5982762/1‑93 (MQ=255)
                                                       gATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAtt                                  <  1:6791589/93‑1 (MQ=255)
                                                       gATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAtt                                  <  2:1204946/93‑1 (MQ=255)
                                                       gATATTGAAACCTGGCGGTAGGATGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGACGCAtt                                  >  2:354899/1‑93 (MQ=255)
                                                        atatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCTTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                                 <  1:7061500/93‑1 (MQ=255)
                                                        atatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                                 <  1:6654988/93‑1 (MQ=255)
                                                         tatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAc                                >  2:1138476/1‑93 (MQ=255)
                                                          atTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcg                               <  1:4072009/93‑1 (MQ=255)
                                                          atTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcg                               <  2:6566572/93‑1 (MQ=255)
                                                           ttGAAACCTGGCGGTAGGCTGATTGTGATGGGCTTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgc                              <  1:3188389/93‑1 (MQ=255)
                                                             gAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGa                            <  2:6653022/93‑1 (MQ=255)
                                                             gAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGa                            <  1:6573224/93‑1 (MQ=255)
                                                             gAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGa                            <  1:2853779/93‑1 (MQ=255)
                                                             gAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGa                            <  2:3136416/93‑1 (MQ=255)
                                                              aaaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGAt                           <  1:1119791/93‑1 (MQ=255)
                                                               aaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATa                          >  2:5160143/1‑93 (MQ=255)
                                                               aaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATa                          <  1:7551413/93‑1 (MQ=255)
                                                                 ccTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAc                                <  1:4592350/85‑1 (MQ=255)
                                                                 ccTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc                        <  1:5028564/93‑1 (MQ=255)
                                                                   tGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctc                      <  1:1224061/93‑1 (MQ=255)
                                                                    ggCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct                     <  2:7264836/93‑1 (MQ=255)
                                                                      cGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCa                   <  2:1807238/93‑1 (MQ=255)
                                                                       ggTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCAc                  >  1:2781904/1‑93 (MQ=255)
                                                                        gTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACg                 >  1:3665185/1‑93 (MQ=255)
                                                                         tAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGt                >  2:2137957/1‑93 (MQ=255)
                                                                          aGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTa               <  2:3229623/93‑1 (MQ=255)
                                                                          aGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTa               >  2:2514357/1‑93 (MQ=255)
                                                                           ggCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTAc              >  2:3784826/1‑93 (MQ=255)
                                                                           ggCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTAc              >  1:3087125/1‑93 (MQ=255)
                                                                              tGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaa           >  2:7893143/1‑93 (MQ=255)
                                                                               taTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          <  1:5850790/92‑1 (MQ=255)
                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          <  2:1518717/93‑1 (MQ=255)
                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          >  2:4652739/1‑93 (MQ=255)
                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          >  1:3836308/1‑93 (MQ=255)
                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          <  1:7640946/93‑1 (MQ=255)
                                                                                 ttGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACt        <  1:5424576/93‑1 (MQ=255)
                                                                                 ttGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACt        <  1:3993514/93‑1 (MQ=255)
                                                                                   gtgATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTAc      <  2:7391446/93‑1 (MQ=255)
                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  >  1:5278095/1‑93 (MQ=255)
                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  <  2:6935743/93‑1 (MQ=255)
                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  <  2:4459126/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCA  >  NC_000913/232128‑232307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑