BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,242	T→C	V192V (GTA→GTG)	b2226 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,242	0	T	C	99.6%	728.9 / ‑5.1	237	V192V (GTA→GTG)	b2226	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (128/108); total (129/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

Only 100 of 248 total aligned reads displayed.

CAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGG  >  NC_000913/2327152‑2327331

caGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCAc                                                                                         <  1:4419193/93‑1 (MQ=255)

 aGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCAcc                                                                                        <  2:2256322/93‑1 (MQ=255)

    ggTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGa                                                                                     >  1:4753419/1‑93 (MQ=255)

     gtgtACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGAc                                                                                    <  1:3348262/93‑1 (MQ=255)

     gtgtACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGAc                                                                                    >  2:2811338/1‑93 (MQ=255)

     gtgtACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGAc                                                                                    <  1:2666236/93‑1 (MQ=255)

     gtgtACCATCGGTTAACCAGCCAGCAAAAACACCCTGACGGATTTCCTGTTTATCATCGGCAACCCAGCTCCAGGTTTTCACCCGCACCCGAc                                                                                    <  1:5981123/93‑1 (MQ=255)

      tgtACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACt                                                                                   >  2:2776927/1‑93 (MQ=255)

        tACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTGCACCCGCACCCGACTAc                                                                                 >  1:7043835/1‑93 (MQ=255)

         aCCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTAcc                                                                                >  2:3833304/1‑93 (MQ=255)

          ccATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCg                                                                               >  2:1715193/1‑93 (MQ=255)

          ccATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCg                                                                               <  2:3405098/93‑1 (MQ=255)

            aTCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAg                                                                             >  2:4827725/1‑93 (MQ=255)

            aTCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAg                                                                             <  2:5544709/93‑1 (MQ=255)

            aTCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAg                                                                             >  2:3053646/1‑93 (MQ=255)

             tCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGc                                                                            <  1:3158359/93‑1 (MQ=255)

             tCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGc                                                                            >  2:5693853/1‑93 (MQ=255)

             tCGGTTAACCAGCCAGCAAAACCACCCTGACGGAGTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGc                                                                            >  2:4956221/1‑93 (MQ=255)

              cGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCa                                                                           <  1:5469912/93‑1 (MQ=255)

              cGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCa                                                                           <  2:7559388/93‑1 (MQ=255)

               ggTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCAt                                                                          >  2:6662066/1‑93 (MQ=255)

                gTTAGCCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATg                                                                         <  1:864982/93‑1 (MQ=255)

                gTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGCGCATg                                                                         >  1:232881/1‑93 (MQ=255)

                gTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATg                                                                         >  1:7009255/1‑93 (MQ=255)

                 ttAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATgg                                                                        <  1:6588075/93‑1 (MQ=255)

                  tAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGa                                                                       <  1:1504242/93‑1 (MQ=255)

                    aCCAGCCAGCAAACCCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATg                                                                     <  2:3560628/93‑1 (MQ=255)

                     ccagccagCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGc                                                                    <  2:7656360/93‑1 (MQ=255)

                           agcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACAcc                                                              >  1:5718313/1‑93 (MQ=255)

                             cAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGa                                                            <  2:7074352/93‑1 (MQ=255)

                              aacaccaccCTGACGGATTTCCTGTTTATCATCGGCAACCCAGCTCCAGGTTTTCGCCCGCACCCGACTACCGAGCATGGATGCGACACCGAt                                                           <  2:7008798/90‑1 (MQ=255)

                               aaaCCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGAtt                                                          <  2:1339300/93‑1 (MQ=255)

                               aaaCCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGAtt                                                          >  2:7911371/1‑93 (MQ=255)

                                aaCCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGAttt                                                         <  1:7618779/93‑1 (MQ=255)

                                 accaccCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGAtttt                                                        >  2:3479809/1‑93 (MQ=255)

                                 accaccCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGAtttt                                                        <  2:6232415/93‑1 (MQ=255)

                                  cgaccCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTc                                                       <  2:3995078/91‑1 (MQ=255)

                                  ccaccCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTc                                                       <  1:3914491/93‑1 (MQ=255)

                                    accCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGc                                                     >  2:5107984/1‑93 (MQ=255)

                                    accCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGc                                                     >  1:1140251/1‑93 (MQ=255)

                                     cccTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGcc                                                    >  1:2178222/1‑93 (MQ=255)

                                       cTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTc                                                  <  2:3244235/93‑1 (MQ=255)

                                        tGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCg                                                 >  1:7066623/1‑93 (MQ=255)

                                            ggATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCa                                             <  2:1573548/93‑1 (MQ=255)

                                             gATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAg                                            >  1:1338729/1‑93 (MQ=255)

                                             gATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAg                                            <  2:6378428/93‑1 (MQ=255)

                                               tttCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCGCCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGca                                          <  2:5909155/93‑1 (MQ=255)

                                                ttCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcac                                         <  2:3661674/93‑1 (MQ=255)

                                                    tGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACcaca                                     <  2:1265189/93‑1 (MQ=255)

                                                    tGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACcaca                                     >  2:2134174/1‑93 (MQ=255)

                                                     gTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACAt                                    <  1:2437129/93‑1 (MQ=255)

                                                       ttATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATcc                                  <  2:1917590/93‑1 (MQ=255)

                                                        tATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCa                                 >  1:6840618/1‑93 (MQ=255)

                                                        tATCATCGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCa                                 >  2:2725013/1‑93 (MQ=255)

                                                         atcatcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAg                                <  2:6110448/93‑1 (MQ=255)

                                                         atcatcGGCAAACCAGCTCCAGGATTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAg                                <  2:4281509/93‑1 (MQ=255)

                                                          tcatcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGt                               >  1:1536845/1‑93 (MQ=255)

                                                           catcGGCAAACCAGCTCCAGGTTTCCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTa                              <  1:6342250/93‑1 (MQ=255)

                                                            atcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTaa                             <  2:4045433/93‑1 (MQ=255)

                                                            atcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTaa                             <  1:5265071/93‑1 (MQ=255)

                                                            atcGGCAAACCAGCGCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTaa                             <  2:4506195/93‑1 (MQ=255)

                                                             tcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGGCTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAg                            >  2:7797909/1‑93 (MQ=255)

                                                             tcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTaaa                            >  2:7152066/1‑92 (MQ=255)

                                                             tcGGCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAg                            <  2:1178458/93‑1 (MQ=255)

                                                               ggCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGAc                          <  1:2952858/93‑1 (MQ=255)

                                                               ggCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGAc                          <  1:185085/93‑1 (MQ=255)

                                                               ggCAAACCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGAc                          <  1:4639624/93‑1 (MQ=255)

                                                                  aaaCCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTc                       >  1:4026605/1‑93 (MQ=255)

                                                                  aaaCCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTc                       <  2:4718403/93‑1 (MQ=255)

                                                                   aaCCAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTcc                      >  2:1363120/1‑93 (MQ=255)

                                                                      cAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGa                   <  1:4262780/93‑1 (MQ=255)

                                                                      cAGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGa                   >  1:4952867/1‑93 (MQ=255)

                                                                       aGCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAg                  >  2:794839/1‑93 (MQ=255)

                                                                        gCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGc                 >  2:6468394/1‑93 (MQ=255)

                                                                        gCTCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGc                 >  2:4387244/1‑93 (MQ=255)

                                                                          tCCAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCtt               >  1:3091878/1‑93 (MQ=255)

                                                                           ccAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCttt              <  1:5965336/93‑1 (MQ=255)

                                                                           ccAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCttt              >  1:163992/1‑93 (MQ=255)

                                                                           ccAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCttt              >  1:2792019/1‑93 (MQ=255)

                                                                            cAGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTg             >  1:1375817/1‑93 (MQ=255)

                                                                            cAGGTTTGCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTg             <  2:243421/93‑1 (MQ=255)

                                                                             aGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACTGATTTTCGCCTCGTCCAGCATCACATCCAGTAAGACTTCCTGAGCTTTGt            <  2:5423615/93‑1 (MQ=255)

                                                                             aGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGt            >  2:2058080/1‑93 (MQ=255)

                                                                             aGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGt            <  2:7537420/93‑1 (MQ=255)

                                                                             aGGTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGt            >  1:6042258/1‑93 (MQ=255)

                                                                              ggTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGtt           >  2:1880374/1‑93 (MQ=255)

                                                                              ggTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGtt           >  1:6145667/1‑93 (MQ=255)

                                                                               gTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGttt          >  2:5563301/1‑93 (MQ=255)

                                                                               gTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGttt          <  2:2463177/93‑1 (MQ=255)

                                                                               gTTTTCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGttt          <  1:3488682/93‑1 (MQ=255)

                                                                                ttttCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGtttt         <  2:7332740/93‑1 (MQ=255)

                                                                                ttttCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGtttt         <  1:6541946/93‑1 (MQ=255)

                                                                                ttttCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGtttt         >  2:2702291/1‑93 (MQ=255)

                                                                                ttttCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGtttt         <  2:2993561/93‑1 (MQ=255)

                                                                                 tttCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTg        >  2:5199728/1‑93 (MQ=255)

                                                                                   tCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTgcg      >  2:6827205/1‑93 (MQ=255)

                                                                                   tCACCCGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACACCCAGTAAGACTTCCTGAGCTTTGTTTTgcg      >  1:5554194/1‑93 (MQ=255)

                                                                                      cccGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCgg   >  1:976783/1‑93 (MQ=255)

                                                                                      cccGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCgg   <  2:5298263/93‑1 (MQ=255)

                                                                                       ccGCACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCggg  >  2:3341543/1‑93 (MQ=255)

CAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGG  >  NC_000913/2327152‑2327331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`