BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,349,989	T→C	M136V (ATG→GTG)	glpT ←	sn‑glycerol‑3‑phosphate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,349,989	0	T	C	100.0%	607.1 / NA	194	M136V (ATG→GTG)	glpT	sn‑glycerol‑3‑phosphate permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (94/100); total (94/100)

Only 100 of 220 total aligned reads displayed.

TGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑T‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCA  >  NC_000913/2349899‑2350078

tgtgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCcacccca‑c‑cc                                                                                         >  1:6291055/1‑93 (MQ=255)

  tgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑cccc                                                                                       >  2:2973014/1‑93 (MQ=255)

  tgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑cccc                                                                                       <  2:4036664/93‑1 (MQ=255)

    cgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTg                                                                                     <  2:504741/93‑1 (MQ=255)

     gcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTgg                                                                                    >  1:2102263/1‑93 (MQ=255)

        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCTACTACCAGTGCACCATAGTACGACCACACGGCCGCCACCCCA‑C‑CCCCTGGaac                                                                                 >  2:2784794/1‑93 (MQ=255)

        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGaac                                                                                 <  1:5530565/93‑1 (MQ=255)

        caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGaac                                                                                 <  1:2183560/93‑1 (MQ=255)

         aGTTCCACACTGACACAATGCCTCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGaacc                                                                                <  1:1800117/93‑1 (MQ=255)

          gTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGaacc                                                                                >  1:3431256/1‑93 (MQ=255)

            tCCACACTGCCACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCC‑C‑CCCCTGGaaccaac                                                                             <  1:1665412/93‑1 (MQ=255)

            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGaaccaa                                                                              >  1:1872555/1‑93 (MQ=255)

            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGaaccaac                                                                             >  1:7184189/1‑93 (MQ=255)

            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGaaccaac                                                                             <  1:4129421/93‑1 (MQ=255)

            tCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGaaccaac                                                                             <  1:2742521/93‑1 (MQ=255)

                cacTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCCCCCCACC‑CCCCTGGAACCAACCGc                                                                          >  2:525628/1‑93 (MQ=255)

                cacTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCa                                                                         >  1:7321478/1‑93 (MQ=255)

                cacTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCa                                                                         <  1:595762/93‑1 (MQ=255)

                 acTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑CCCCCCTGGAACCAACCGCa                                                                         <  1:6150825/93‑1 (MQ=255)

                 acTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCag                                                                        >  2:1295577/1‑93 (MQ=255)

                    gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAgg                                                                     >  2:427729/1‑93 (MQ=255)

                    gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAgg                                                                     <  1:33265/93‑1 (MQ=255)

                     acacAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCACCCGCCGAgg                                                                     >  1:2349863/1‑93 (MQ=255)

                     acacAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGa                                                                    <  2:7574903/93‑1 (MQ=255)

                     acacAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGa                                                                    <  2:2034451/93‑1 (MQ=255)

                     acacAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGa                                                                    <  1:2635281/93‑1 (MQ=255)

                       tcaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCTCA‑C‑CCCCTGGAACCAACCGCAGAGGaac                                                                  >  1:7023295/2‑93 (MQ=255)

                       acaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑CCCCCCTGGAACCAACCGCAGAGGaa                                                                   <  2:1117125/93‑1 (MQ=255)

                       acaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGaac                                                                  >  1:2691749/1‑93 (MQ=255)

                         aaTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGaaca                                                                 >  2:422793/1‑93 (MQ=255)

                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGaacaac                                                               <  2:6825625/93‑1 (MQ=255)

                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGaacaac                                                               >  2:4741317/1‑93 (MQ=255)

                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGaacaac                                                               >  2:4852132/1‑93 (MQ=255)

                            gccgccACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑CCCCCCTGGAACCAACCGCAGAGGAacaaca                                                              <  1:5625238/93‑1 (MQ=255)

                             ccgccACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAGCAACAGt                                                            >  2:5855538/1‑93 (MQ=255)

                              cgccACGTTCTTTCTGCGACCACCAGTGTACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTa                                                           >  2:5196745/1‑93 (MQ=255)

                               gccACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTaa                                                          >  1:2884184/1‑92 (MQ=255)

                               gccACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTAc                                                          >  1:5166051/1‑93 (MQ=255)

                                ccACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACa                                                         >  2:2080985/1‑93 (MQ=255)

                                 cACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACaa                                                        >  1:801782/1‑93 (MQ=255)

                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       >  2:3141802/1‑93 (MQ=255)

                                  aCGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       >  2:6609075/1‑93 (MQ=255)

                                  aCGTTCTTTCTGCCACCACCAGTGCACCATAGTACGACCACGCGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACaaa                                                       <  1:7078164/93‑1 (MQ=255)

                                    gTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACaaaa                                                      >  2:1778750/1‑92 (MQ=255)

                                    gTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAAc                                                      >  1:3845185/1‑93 (MQ=255)

                                    gTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACa                                                     <  2:1210491/93‑1 (MQ=255)

                                          tctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCg                                               <  1:6224454/93‑1 (MQ=255)

                                           ctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGc                                              <  1:1353751/93‑1 (MQ=255)

                                             gCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCaa                                            <  1:2292736/93‑1 (MQ=255)

                                             gCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCaa                                            <  1:328748/93‑1 (MQ=255)

                                             gCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCaa                                            <  2:349213/93‑1 (MQ=255)

                                              cGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAt                                           <  1:5482614/93‑1 (MQ=255)

                                               gACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATg                                          >  1:540351/1‑93 (MQ=255)

                                               gACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATg                                          >  1:425997/1‑93 (MQ=255)

                                                 ccaccaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCt                                        <  2:3708209/93‑1 (MQ=255)

                                                  caccaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTc                                       <  1:6793308/93‑1 (MQ=255)

                                                  caccaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTc                                       <  2:5906162/93‑1 (MQ=255)

                                                  caccaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTc                                       <  1:3149727/93‑1 (MQ=255)

                                                  caccaGTGCAACATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTc                                       <  2:2559389/93‑1 (MQ=255)

                                                   accaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTca                                      >  1:497174/1‑92 (MQ=255)

                                                    cgaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCGGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGa                                     <  1:7210584/91‑1 (MQ=255)

                                                    ccaGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGa                                     <  1:7288248/93‑1 (MQ=255)

                                                      aGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACg                                   >  1:3625624/1‑93 (MQ=255)

                                                        tGCACCATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTc                                 <  2:2635340/93‑1 (MQ=255)

                                                         gCACCATAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTc                                 >  2:623018/1‑93 (MQ=255)

                                                            ccATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGccc                             <  1:668559/93‑1 (MQ=255)

                                                             cATAGTACGACCACACGGCGGCCCCCCCC‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCa                            <  1:377397/93‑1 (MQ=255)

                                                             cATAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCa                            <  1:6480026/93‑1 (MQ=255)

                                                              aTAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCa                            <  2:866606/93‑1 (MQ=255)

                                                              aTAGTACGACCACACGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCa                            >  2:7850544/1‑93 (MQ=255)

                                                              aTAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCAt                           >  2:678334/1‑93 (MQ=255)

                                                               tAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          >  1:1290980/1‑93 (MQ=255)

                                                               tAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          <  1:4460290/93‑1 (MQ=255)

                                                               tAGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATg                          <  2:3894938/93‑1 (MQ=255)

                                                                aGTACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATgg                         >  2:7009070/1‑93 (MQ=255)

                                                                  tACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACACACATCACCGCAATGCTCGACGTCGCCCATGGca                       >  1:7632849/1‑93 (MQ=255)

                                                                  tACGACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGca                       >  2:581493/1‑93 (MQ=255)

                                                                   aCGACCACACGGCGGCCCCCCCC‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGcac                      <  1:6769225/93‑1 (MQ=255)

                                                                     gACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACaa                    >  2:3454697/1‑93 (MQ=255)

                                                                     gACCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACaa                    <  1:1927185/93‑1 (MQ=255)

                                                                      aCCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACaaa                   <  2:4286090/93‑1 (MQ=255)

                                                                      aCCACACGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACaaa                   >  1:2683198/1‑93 (MQ=255)

                                                                        cacaCGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGc                 <  1:488764/93‑1 (MQ=255)

                                                                         ccacGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGcc                <  2:760652/92‑1 (MQ=255)

                                                                         acacGGGGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGcc                >  2:7678627/1‑93 (MQ=255)

                                                                         acacGGCGGCCGCCCCA‑C‑CCCCTGGAACCAACGGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCAGGGCACAAAGcc                <  2:257012/93‑1 (MQ=255)

                                                                         acacGGCGGCCACCCCACC‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGc                 >  1:5734184/1‑93 (MQ=255)

                                                                         acacGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGcc                >  1:4759079/1‑93 (MQ=255)

                                                                         acacGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGcc                >  1:1053228/1‑93 (MQ=255)

                                                                           acGGCGGCCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCa              <  1:5196648/93‑1 (MQ=255)

                                                                             ggcggcCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATa            <  1:1523381/93‑1 (MQ=255)

                                                                               cggcCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACGAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATaaa          <  2:326591/93‑1 (MQ=255)

                                                                               cggcCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATaaa          <  2:5608716/93‑1 (MQ=255)

                                                                                ggcCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAaac         <  1:1201556/93‑1 (MQ=255)

                                                                                 gcCACCCCA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAaaca        >  1:2379839/1‑93 (MQ=255)

                                                                                  ccacccca‑c‑cCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAaacaa       <  2:2883280/93‑1 (MQ=255)

                                                                                     ccccA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACAt    <  2:1095460/93‑1 (MQ=255)

                                                                                      cccA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATc   <  2:6298780/93‑1 (MQ=255)

                                                                                      cccA‑C‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATc   <  2:2374409/93‑1 (MQ=255)

                                                                                       ccA‑C‑CCCCTGGAACCAACCGCATAGGAACAACAGTGCAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCa  >  1:1407542/1‑93 (MQ=255)

TGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCA‑T‑CCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCA  >  NC_000913/2349899‑2350078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`