BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,890	T→C	V105A (GTG→GCG)	b4533 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,890	0	T	C	99.5%	675.0 / NA	215	V105A (GTG→GCG)	b4533	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (91/123); minor base G (1/0); total (92/123)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

TCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGC  >  NC_000913/2370800‑2370979

tCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGa                                                                                         <  2:6377013/93‑1 (MQ=255)

tCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGa                                                                                         <  2:5547493/93‑1 (MQ=255)

tCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGa                                                                                         >  2:3785848/1‑93 (MQ=255)

 cTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGAt                                                                                        <  1:7101531/93‑1 (MQ=255)

  tGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGGGATc                                                                                       >  2:6097248/1‑93 (MQ=255)

  tGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATc                                                                                       >  1:7548444/1‑93 (MQ=255)

   gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATcc                                                                                      <  1:5160395/93‑1 (MQ=255)

   gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATcc                                                                                      <  2:5940810/93‑1 (MQ=255)

   gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATcc                                                                                      >  2:3777499/1‑93 (MQ=255)

   gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATcc                                                                                      <  2:4315324/93‑1 (MQ=255)

    ggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCt                                                                                     <  2:7369804/93‑1 (MQ=255)

     gTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTc                                                                                    <  1:4622675/93‑1 (MQ=255)

     gTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTc                                                                                    <  1:15941/93‑1 (MQ=255)

       gACGCTGTGTGTAGTAAAGCGGTGGCACGAACCGGTATCCCCGCGTCGCTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCgg                                                                                  <  2:3330712/93‑1 (MQ=255)

       gACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCgg                                                                                  <  1:881669/93‑1 (MQ=255)

        aCGCGGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCggg                                                                                 <  2:5048806/93‑1 (MQ=255)

         cGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGa                                                                                <  1:411918/93‑1 (MQ=255)

          gctggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAg                                                                               >  1:5314493/1‑93 (MQ=255)

          gctggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAg                                                                               <  1:3321598/93‑1 (MQ=255)

           ctggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGt                                                                              >  1:7268364/1‑93 (MQ=255)

           ctggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGt                                                                              <  1:795542/93‑1 (MQ=255)

            tggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTa                                                                             >  2:3080231/1‑93 (MQ=255)

            tggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTa                                                                             <  2:1247565/93‑1 (MQ=255)

              gctgCAGTAAAACTGTGGCCCGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACg                                                                           <  1:2556475/93‑1 (MQ=255)

                 gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTTACTGGTGTGGGGTGGGGTTGATTATTGGCGGCATTGCGATCCTCGGGAGTACgggg                                                                        >  1:2896020/1‑91 (MQ=255)

                 gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGgtg                                                                        >  1:2894632/1‑93 (MQ=255)

                 gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGgtg                                                                        >  1:4491555/1‑93 (MQ=255)

                   aGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTa                                                                      <  1:3238827/93‑1 (MQ=255)

                    gTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTaa                                                                     >  1:7749269/1‑93 (MQ=255)

                    gTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTaa                                                                     <  2:6664365/93‑1 (MQ=255)

                    gTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTaa                                                                     >  1:5225878/1‑93 (MQ=255)

                    gTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTaa                                                                     <  2:7841469/93‑1 (MQ=255)

                     tAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTCCGGTGTAAt                                                                    <  2:7762461/93‑1 (MQ=255)

                     tAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAAt                                                                    <  2:3226224/93‑1 (MQ=255)

                     tAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAAt                                                                    <  1:622241/93‑1 (MQ=255)

                     tAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAAt                                                                    >  2:5330337/1‑93 (MQ=255)

                     tAAAACTGCCGCACGAACCCGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAAt                                                                    <  2:7073256/93‑1 (MQ=255)

                      aaaaCTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATg                                                                   <  1:3855999/93‑1 (MQ=255)

                       agccTGGGGCCCGAACCGGTATCGCCGCGCCACTGGTGTGGGGCGGTGTTCATTGTGGGCGGCATCGCGCCCCTCGGGAGTCCGGTTTAATgg                                                                  <  2:2597114/90‑1 (MQ=255)

                        aaCTGTGGCACGAACCGGTATCGCCGCGTCACGGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATggg                                                                 <  1:5669364/93‑1 (MQ=255)

                         aCTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                <  2:7525705/93‑1 (MQ=255)

                         aCTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                <  1:7363384/93‑1 (MQ=255)

                         aCTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                >  2:4419390/1‑93 (MQ=255)

                          cTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGcc                                                               >  2:1082569/1‑93 (MQ=255)

                            gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTg                                                             >  1:1678264/1‑93 (MQ=255)

                            gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTg                                                             >  2:4627294/1‑93 (MQ=255)

                            gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTg                                                             <  1:5722791/93‑1 (MQ=255)

                             tgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGa                                                            >  2:5113448/1‑93 (MQ=255)

                              ggCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGAt                                                           <  2:608159/93‑1 (MQ=255)

                                           aTCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                              <  2:5509370/93‑1 (MQ=255)

                                           aTCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                              >  2:453544/1‑93 (MQ=255)

                                            tCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCa                                             <  1:2378905/93‑1 (MQ=255)

                                            tCGCCGCGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCa                                             >  2:2778602/1‑93 (MQ=255)

                                              gccgcGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGc                                           <  1:480042/93‑1 (MQ=255)

                                              gccgcGTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGc                                           <  1:5889438/93‑1 (MQ=255)

                                                 gcgTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTc                                        >  1:1439317/1‑93 (MQ=255)

                                                   gTCACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCat                                      >  2:6496767/1‑93 (MQ=255)

                                                     cACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatta                                    >  1:157201/1‑93 (MQ=255)

                                                     cACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatta                                    <  1:50500/93‑1 (MQ=255)

                                                     cACTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatta                                    <  1:6484570/93‑1 (MQ=255)

                                                      aCTGGTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCattat                                   >  2:7908647/1‑93 (MQ=255)

                                                       cTGGTGTGGGGGGGTGTTCATTATTGGCGGCATTGCGATCCACGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCattatt                                  <  2:6841496/93‑1 (MQ=255)

                                                         ggTGTGGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGc                                <  1:1133638/93‑1 (MQ=255)

                                                             tgGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCa                            <  2:3201939/93‑1 (MQ=255)

                                                             tgGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCa                            <  1:7030622/93‑1 (MQ=255)

                                                             tgGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCa                            <  2:5691013/93‑1 (MQ=255)

                                                             tgGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCa                            <  2:1546742/93‑1 (MQ=255)

                                                             tgGGGTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCa                            <  1:6090368/93‑1 (MQ=255)

                                                             tgGGGGGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCa                            >  2:7458231/1‑93 (MQ=255)

                                                              ggggTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAg                           <  1:6270933/93‑1 (MQ=255)

                                                               gggTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGt                          <  1:1172574/93‑1 (MQ=255)

                                                               gggTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGt                          <  2:2079907/93‑1 (MQ=255)

                                                               gggTGGTGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGCTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGt                          >  2:725538/1‑93 (MQ=255)

                                                                ggtggtGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGtt                         >  2:3973052/1‑93 (MQ=255)

                                                                 gtggtgTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTg                        >  1:3490324/1‑93 (MQ=255)

                                                                    gtgTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcgc                     >  1:6758124/3‑93 (MQ=255)

                                                                     tgTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCa                    >  2:1025054/2‑93 (MQ=255)

                                                                     tgTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGCTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCa                    >  1:5519648/2‑93 (MQ=255)

                                                                      gTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa                   >  1:2764410/1‑93 (MQ=255)

                                                                      gTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa                   <  2:5865730/93‑1 (MQ=255)

                                                                      gTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa                   <  2:2062094/93‑1 (MQ=255)

                                                                        tCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAtt                 >  2:4147419/1‑93 (MQ=255)

                                                                        tCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAtt                 <  1:1295083/93‑1 (MQ=255)

                                                                        tCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAtt                 >  2:6900619/1‑93 (MQ=255)

                                                                         cATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTa                <  1:1980348/93‑1 (MQ=255)

                                                                          attattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTaa               >  2:3479389/1‑93 (MQ=255)

                                                                          attattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTaa               <  1:6544494/93‑1 (MQ=255)

                                                                           ttattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAg              <  1:2521482/93‑1 (MQ=255)

                                                                             attGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGcc            <  1:1902386/93‑1 (MQ=255)

                                                                              ttGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCt           >  2:2538902/1‑93 (MQ=255)

                                                                                tgcggtATTGTGATCCTCGGGAGTACGTGGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg         <  1:4232134/92‑1 (MQ=255)

                                                                                  cggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGt       <  2:6825863/93‑1 (MQ=255)

                                                                                   ggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtt      <  2:4670797/93‑1 (MQ=255)

                                                                                   ggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtt      >  1:6664132/1‑93 (MQ=255)

                                                                                     cATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtttt    >  1:2833578/1‑93 (MQ=255)

                                                                                      aTTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTg   >  1:1071519/1‑93 (MQ=255)

                                                                                       ttGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc  >  2:4857915/1‑93 (MQ=255)

                                                                                       ttGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc  <  2:1800618/93‑1 (MQ=255)

                                                                                       ttGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc  <  1:1024207/93‑1 (MQ=255)

                                                                                       ttGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc  >  2:824367/1‑93 (MQ=255)

TCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGC  >  NC_000913/2370800‑2370979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`