BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,074	A→G	N145S (AAC→AGC)	yfcG →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,074	0	A	G	98.8%	795.0 / ‑5.3	254	N145S (AAC→AGC)	yfcG	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (134/116); minor base T (2/1); total (137/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

ATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTC  >  NC_000913/2418984‑2419163

catcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCa                                                                                         >  1:1484395‑M2/22‑93 (MQ=255)

catcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCa                                                                                         <  1:797339‑M2/72‑1 (MQ=255)

catcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCa                                                                                         >  2:3311149‑M2/22‑93 (MQ=255)

catcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCa                                                                                         <  2:3954976‑M2/72‑1 (MQ=255)

 atcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCaa                                                                                        <  1:5132142‑M2/73‑1 (MQ=255)

   cattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGc                                                                                      <  1:4184280‑M2/75‑1 (MQ=255)

    attttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCg                                                                                     >  1:4583182‑M2/18‑93 (MQ=255)

         aatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                >  1:7861793‑M2/13‑93 (MQ=255)

          atcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGa                                                                               >  2:7240649‑M2/12‑93 (MQ=255)

            cacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGaaa                                                                             <  1:4879981‑M2/84‑1 (MQ=255)

            cacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGaaa                                                                             <  2:669167‑M2/84‑1 (MQ=255)

              cagccccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAAc                                                                           >  2:5842494‑M2/8‑93 (MQ=255)

               cagcccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACt                                                                          >  1:5963343‑M2/7‑93 (MQ=255)

                   ccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGcc                                                                      <  2:483135‑M2/91‑1 (MQ=255)

                    cCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGccc                                                                     >  1:6759503‑M2/2‑93 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCct                                                                    <  1:6445364/93‑1 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGGCTTTACCATGTACTGATCAAGCGGCTGGAACACTCGCCct                                                                    >  2:4367782/1‑93 (MQ=255)

                      cccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctg                                                                   >  1:4891045/1‑93 (MQ=255)

                       ccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTGCCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctgg                                                                  <  1:2723067/93‑1 (MQ=255)

                       ccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctgg                                                                  <  1:2827638/93‑1 (MQ=255)

                         ccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggct                                                                <  1:5776093/93‑1 (MQ=255)

                         ccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggct                                                                >  2:2715978/1‑93 (MQ=255)

                         ccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGACctggct                                                                >  1:7224736/1‑93 (MQ=255)

                          cAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggctg                                                               <  2:3397366/93‑1 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggctgg                                                              >  1:6051477/1‑93 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggctgg                                                              <  1:2459413/93‑1 (MQ=255)

                            aaCCATTCCTTACGCTATTGAATGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTATTGAGCAAGCGTCTGGAAAACTCGCCCTGGCTggg                                                             >  2:4316028/1‑93 (MQ=255)

                            aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTggg                                                             >  1:987249/1‑93 (MQ=255)

                            aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTggg                                                             >  2:6359319/1‑93 (MQ=255)

                            aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTggg                                                             >  2:7358866/1‑93 (MQ=255)

                            aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTggg                                                             >  2:7665892/1‑93 (MQ=255)

                            aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTggg                                                             <  1:495509/93‑1 (MQ=255)

                             aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGa                                                            <  1:7747064/93‑1 (MQ=255)

                                aTTCCTTAGGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGc                                                         <  2:2538446/93‑1 (MQ=255)

                                aTTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGc                                                         <  2:4205782/93‑1 (MQ=255)

                                 ttCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCg                                                        >  1:6184295/1‑93 (MQ=255)

                                 ttCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTCGCTGGGAGGCg                                                        >  2:4584579/1‑93 (MQ=255)

                                  tCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCga                                                       >  2:633019/1‑93 (MQ=255)

                                  tCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCga                                                       >  2:1229122/1‑93 (MQ=255)

                                   ccTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCgag                                                      <  1:188770/93‑1 (MQ=255)

                                    cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCgaga                                                     >  1:5201143/1‑93 (MQ=255)

                                    cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCgaga                                                     <  1:2659554/93‑1 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGaa                                                    <  2:6642929/93‑1 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGaa                                                    >  1:3274758/1‑93 (MQ=255)

                                      tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGATCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAAc                                                   <  2:1946466/93‑1 (MQ=255)

                                         gCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTAc                                                >  1:2374116/1‑93 (MQ=255)

                                          cTATTGAACGTTATCAGGTTGAAACTCATCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACa                                               <  2:3280858/93‑1 (MQ=255)

                                             ttGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCa                                            <  1:5631079/93‑1 (MQ=255)

                                             ttGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCa                                            <  1:6925929/93‑1 (MQ=255)

                                              tGACCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGTCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAt                                           <  1:7345735/93‑1 (MQ=255)

                                              tGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAt                                           <  2:6033140/93‑1 (MQ=255)

                                              tGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAt                                           <  2:7325785/93‑1 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          <  2:2898389/93‑1 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          <  2:2433420/93‑1 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          >  1:4523796/1‑93 (MQ=255)

                                                aaCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTg                                         >  1:7434466/1‑93 (MQ=255)

                                                 aCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGc                                        >  2:3435119/1‑93 (MQ=255)

                                                   gTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCgg                                      >  2:5493010/1‑93 (MQ=255)

                                                    ttATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGa                                     >  2:5667777/1‑93 (MQ=255)

                                                    ttATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGa                                     <  1:4712396/93‑1 (MQ=255)

                                                    ttATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGa                                     >  2:4065856/1‑93 (MQ=255)

                                                     tATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGat                                    >  2:1123870/1‑93 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   <  1:6299664/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   <  2:4525804/93‑1 (MQ=255)

                                                       tCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGatat                                  >  2:5969891/1‑93 (MQ=255)

                                                        cAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATAtt                                 <  2:4253938/93‑1 (MQ=255)

                                                          ggTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGc                               <  1:3070733/93‑1 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              >  1:3757550/1‑93 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              <  1:1645703/93‑1 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              <  1:7021276/93‑1 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  2:5760348/93‑1 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  2:1928534/93‑1 (MQ=255)

                                                             tGACACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctg                            <  2:6249048/93‑1 (MQ=255)

                                                             tGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctg                            <  1:1264611/93‑1 (MQ=255)

                                                              gAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                           <  2:1482321/93‑1 (MQ=255)

                                                              gAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                           <  1:7639053/93‑1 (MQ=255)

                                                               aaaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgct                          <  1:3916372/93‑1 (MQ=255)

                                                               aaaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgct                          >  2:6018394/1‑93 (MQ=255)

                                                                aaCTCAGCGTTTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         <  2:5069927/93‑1 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         >  1:162506/1‑93 (MQ=255)

                                                                 aCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTgg                        <  2:227154/93‑1 (MQ=255)

                                                                  cTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGc                       <  1:3835587/93‑1 (MQ=255)

                                                                   tCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGcc                      >  2:2734358/1‑93 (MQ=255)

                                                                   tCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGcc                      >  1:1081084/1‑93 (MQ=255)

                                                                     aGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCgg                    >  1:7473399/1‑92 (MQ=255)

                                                                       cGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                  <  1:6593076/93‑1 (MQ=255)

                                                                        gTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTggg                 >  1:1823164/1‑93 (MQ=255)

                                                                        gTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTggg                 >  2:1876098/1‑93 (MQ=255)

                                                                         acTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGt                <  1:5333805/92‑1 (MQ=255)

                                                                           tttACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTa              >  1:6495641/1‑93 (MQ=255)

                                                                           tttACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTa              >  1:2927196/1‑93 (MQ=255)

                                                                            ttACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTaa             >  1:2193380/1‑93 (MQ=255)

                                                                             tACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAt            <  1:1368499/93‑1 (MQ=255)

                                                                                cATGTACTGAGCAAGCGGCTGGAAAGCTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc         >  2:234344/1‑93 (MQ=255)

                                                                                 aTGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt        <  2:1556773/93‑1 (MQ=255)

                                                                                  tGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTg       <  1:2593965/93‑1 (MQ=255)

                                                                                    tACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGa     >  2:7135034/1‑93 (MQ=255)

                                                                                     aCTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGAc    <  1:3486811/93‑1 (MQ=255)

                                                                                      cTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACt   <  2:6907073/93‑1 (MQ=255)

                                                                                       tGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTc  <  2:1075129/93‑1 (MQ=255)

ATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTC  >  NC_000913/2418984‑2419163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`