BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,461,706	T→C	S225S (AGT→AGC)	b2345 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,461,706	0	T	C	99.7%	999.4 / ‑5.8	313	S225S (AGT→AGC)	b2345	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (162/150); total (162/151)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 315 total aligned reads displayed.

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  2:3032392/1‑93 (MQ=255)

  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  1:2280694/1‑93 (MQ=255)

  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  2:2352825/1‑93 (MQ=255)

  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  1:3229844/1‑93 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  2:4623109/93‑1 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     <  2:4481614/93‑1 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     >  1:1042030/1‑93 (MQ=255)

     tgtctctcGTTTACTACGATCA‑GACGACTACCACATTCAAAGAGGATTTCGTATTAAGGGTCATAGTCTAACCCAATATTGTAGCGAAAACGc                                                                                   <  2:4943644/91‑1 (MQ=255)

      ttctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGc                                                                                   <  1:1725789/92‑1 (MQ=255)

      aTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGc                                                                                   >  1:2808549/1‑93 (MQ=255)

      aTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGc                                                                                   >  1:4342789/1‑93 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCCATATTGTAGCGAAAACGcc                                                                                  <  1:7663678/93‑1 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  <  1:3580935/93‑1 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  >  1:6659824/1‑93 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  >  2:5198554/1‑93 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  >  2:1531170/1‑93 (MQ=255)

        ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCa                                                                                 <  2:3214710/93‑1 (MQ=255)

         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                >  1:3670856/1‑93 (MQ=255)

         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                <  2:2884095/93‑1 (MQ=255)

         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                <  1:7518692/93‑1 (MQ=255)

          ctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAgg                                                                               >  2:7658990/1‑93 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAggg                                                                              >  2:6051071/1‑92 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              >  2:1347252/1‑93 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              <  1:980322/93‑1 (MQ=255)

             ggTTACAACGATCAGGACGACTACCACGTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGt                                                                            >  2:7785018/1‑93 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  1:2851350/1‑93 (MQ=255)

                tactacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCt                                                                         <  2:7533195/93‑1 (MQ=255)

                tactacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCt                                                                         >  1:4055755/1‑93 (MQ=255)

                 actacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCtt                                                                        >  2:1455153/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      <  2:642435/93‑1 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      <  2:1704005/93‑1 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      >  2:3728962/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      >  1:7411295/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      <  1:3735486/93‑1 (MQ=255)

                     cGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGc                                                                    <  2:4311624/93‑1 (MQ=255)

                      gATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCg                                                                   >  2:7276933/1‑93 (MQ=255)

                      gATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCg                                                                   >  2:6756366/1‑93 (MQ=255)

                       aTCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGt                                                                  <  1:7547395/93‑1 (MQ=255)

                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 >  1:6242538/1‑93 (MQ=255)

                          aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGt                                                               <  1:4116260/93‑1 (MQ=255)

                           ggACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                              >  1:5926008/1‑93 (MQ=255)

                           ggACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                              >  2:3588926/1‑93 (MQ=255)

                           ggACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                              <  1:925405/93‑1 (MQ=255)

                             acgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTg                                                            <  1:7112162/93‑1 (MQ=255)

                                  tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                       >  2:5807521/1‑93 (MQ=255)

                                   aCCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCa                                                      >  1:631887/1‑93 (MQ=255)

                                   aCCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCa                                                      >  2:5143284/1‑93 (MQ=255)

                                    ccACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAg                                                     <  1:4509668/93‑1 (MQ=255)

                                    ccACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAg                                                     >  1:256420/1‑93 (MQ=255)

                                      ccaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTa                                                   <  2:318805/92‑1 (MQ=255)

                                      acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTa                                                   >  2:2288714/1‑93 (MQ=255)

                                        aTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                 >  2:1912818/1‑93 (MQ=255)

                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                >  2:1615003/1‑93 (MQ=255)

                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                <  2:2560070/93‑1 (MQ=255)

                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                >  1:7867002/1‑93 (MQ=255)

                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                >  1:6915959/1‑93 (MQ=255)

                                          tCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGc                                               <  2:6550255/93‑1 (MQ=255)

                                            aaaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTa                                             <  2:5675064/93‑1 (MQ=255)

                                             aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAt                                            <  2:5346213/93‑1 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          <  1:1300381/93‑1 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  1:5849979/1‑93 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          <  1:5600862/93‑1 (MQ=255)

                                                aagAATTCGTATTAATGGTCATAGTCTCACCAAATATTGTAGCGAAATCGCAAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                         <  2:331793/91‑1 (MQ=255)

                                                 ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                        >  2:6925435/1‑93 (MQ=255)

                                                 ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                        <  2:6158111/93‑1 (MQ=255)

                                                  gAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATgg                                       >  1:4578258/1‑93 (MQ=255)

                                                    aTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCa                                     >  2:2130908/1‑93 (MQ=255)

                                                      tCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGa                                   <  1:4658382/93‑1 (MQ=255)

                                                       cGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGaa                                  <  2:2703771/93‑1 (MQ=255)

                                                        gTATTAATGGTGATAGTCTTACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                 <  2:4786294/93‑1 (MQ=255)

                                                         tATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATc                                >  1:124746/1‑93 (MQ=255)

                                                            tAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAtt                             >  2:5937562/1‑93 (MQ=255)

                                                             aaTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                            >  2:4160889/1‑93 (MQ=255)

                                                             aaTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                            <  2:5762616/93‑1 (MQ=255)

                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           >  2:6093877/1‑93 (MQ=255)

                                                               tGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGc                          <  2:1788789/93‑1 (MQ=255)

                                                                 gTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaa                        <  1:1117447/93‑1 (MQ=255)

                                                                 gTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaa                        <  2:5490881/93‑1 (MQ=255)

                                                                   gATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACt                      <  1:6538070/93‑1 (MQ=255)

                                                                   gATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACt                      >  2:6825216/1‑93 (MQ=255)

                                                                    aTAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTg                     <  1:2398494/93‑1 (MQ=255)

                                                                     tAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                    >  1:1902074/1‑93 (MQ=255)

                                                                     tAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                    <  1:2479913/93‑1 (MQ=255)

                                                                       gTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGtt                  <  1:3376005/93‑1 (MQ=255)

                                                                        tctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTc                 >  2:573402/1‑93 (MQ=255)

                                                                        tctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTc                 <  2:7059718/93‑1 (MQ=255)

                                                                         ctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTcc                >  1:302643/1‑93 (MQ=255)

                                                                             cccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc            >  1:1435388/1‑93 (MQ=255)

                                                                              ccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatca           >  1:5053710/1‑93 (MQ=255)

                                                                              ccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatca           <  2:6516393/93‑1 (MQ=255)

                                                                               cAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcat          >  1:4763512/1‑93 (MQ=255)

                                                                                aaTATTGTAGCGAAAACGCCAGGTGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGACAGAATCATTAGCAACTGGTTCCGatcatc         <  2:5397693/93‑1 (MQ=255)

                                                                                aaTATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcatc         >  2:7327339/1‑93 (MQ=255)

                                                                                aaTATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcatc         <  1:6482487/93‑1 (MQ=255)

                                                                                aaTATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTCGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcatc         <  2:4055754/93‑1 (MQ=255)

                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        <  1:3502426/93‑1 (MQ=255)

                                                                                  tatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAg       <  2:3551878/93‑1 (MQ=255)

                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     <  1:681788/93‑1 (MQ=255)

                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     >  2:7691262/1‑93 (MQ=255)

                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     <  1:5296630/93‑1 (MQ=255)

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`