BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,464,010	T→C	H230H (CAT→CAC)	yfdC →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,464,010	0	T	C	99.6%	714.7 / ‑5.0	228	H230H (CAT→CAC)	yfdC	putative transport
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (117/110); total (117/111)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 246 total aligned reads displayed.

TATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCACTACCTAC  >  NC_000913/2463920‑2464099

tATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCacat                                                                                         >  1:5675912/1‑93 (MQ=255)

tATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCacat                                                                                         >  2:6370038/1‑93 (MQ=255)

tATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCacat                                                                                         >  1:7805340/1‑93 (MQ=255)

tATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCacat                                                                                         <  2:2532848/93‑1 (MQ=255)

       tGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTc                                                                                  <  1:4269054/93‑1 (MQ=255)

         gATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCgg                                                                                <  1:2058462/93‑1 (MQ=255)

         gATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCgg                                                                                >  2:4165078/1‑93 (MQ=255)

         gATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCgg                                                                                <  1:2544789/93‑1 (MQ=255)

          aTGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGt                                                                               <  1:7222373/93‑1 (MQ=255)

          aTGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGt                                                                               <  2:3565867/93‑1 (MQ=255)

           tGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGtt                                                                              <  1:264708/93‑1 (MQ=255)

            gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTc                                                                             >  1:3677433/1‑93 (MQ=255)

               tCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGt                                                                          >  2:7111414/1‑93 (MQ=255)

               tCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGt                                                                          <  2:3226645/93‑1 (MQ=255)

                 cTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTg                                                                        >  1:2747515/1‑93 (MQ=255)

                  tGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGa                                                                       <  1:658503/93‑1 (MQ=255)

                   gCAGCGGGTGCGGCAAGGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGaa                                                                      <  1:4761567/93‑1 (MQ=255)

                   gCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGaa                                                                      >  1:5905315/1‑93 (MQ=255)

                    cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGaaa                                                                     >  2:6215439/1‑93 (MQ=255)

                    cAGCGGGTACGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTATTGaaa                                                                     >  2:4907349/1‑93 (MQ=255)

                      gCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATc                                                                   >  1:6290790/1‑93 (MQ=255)

                      gCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATc                                                                   <  2:1944466/93‑1 (MQ=255)

                        gggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCct                                                                 <  1:2982298/93‑1 (MQ=255)

                        gggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCct                                                                 <  2:5905331/93‑1 (MQ=255)

                        gggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCct                                                                 <  1:986804/93‑1 (MQ=255)

                         ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctc                                                                <  2:5036203/93‑1 (MQ=255)

                          gTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctct                                                               >  1:1164805/1‑93 (MQ=255)

                          gTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCctct                                                               <  2:5628459/93‑1 (MQ=255)

                             cGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATc                                                            <  2:1613444/93‑1 (MQ=255)

                             cGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATc                                                            <  2:2859180/93‑1 (MQ=255)

                              ggCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCt                                                           >  1:4048139/1‑93 (MQ=255)

                               gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                                          >  1:3356495/1‑93 (MQ=255)

                               gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                                          >  2:3917511/1‑93 (MQ=255)

                                gaaaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTgg                                                         <  1:6077678/92‑1 (MQ=255)

                                cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTgg                                                         >  2:2052759/1‑93 (MQ=255)

                                 aaaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgt                                                        <  1:7559523/93‑1 (MQ=255)

                                 aaaGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgt                                                        <  1:2133598/93‑1 (MQ=255)

                                   aGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgtgt                                                      <  1:1187849/93‑1 (MQ=255)

                                   aGATTGTGGTGATTATATTGAGGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgtgt                                                      >  1:1331318/1‑93 (MQ=255)

                                     aTTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                                    >  1:4977375/1‑93 (MQ=255)

                                        gtggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                                                 <  1:505706/93‑1 (MQ=255)

                                        gtggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                                                 <  1:6388356/93‑1 (MQ=255)

                                         tggttATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACg                                                <  1:5849522/93‑1 (MQ=255)

                                         tggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACg                                                <  2:6714607/93‑1 (MQ=255)

                                          ggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACgg                                               <  1:5207639/93‑1 (MQ=255)

                                           gtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGt                                              >  1:666881/1‑93 (MQ=255)

                                           gtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGt                                              <  1:2847660/93‑1 (MQ=255)

                                            tgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTa                                             >  2:7605353/1‑93 (MQ=255)

                                             gATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTAc                                            >  1:3261183/1‑93 (MQ=255)

                                              aTTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACg                                           >  2:2101750/1‑93 (MQ=255)

                                              aTTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACg                                           >  2:5409202/1‑93 (MQ=255)

                                               ttATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc                                          >  1:6451821/1‑93 (MQ=255)

                                                tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCt                                         <  1:1577533/93‑1 (MQ=255)

                                                  tatTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGc                                       <  2:2321730/93‑1 (MQ=255)

                                                   atTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCa                                      >  1:5359966/1‑93 (MQ=255)

                                                   atTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCa                                      <  1:7872443/93‑1 (MQ=255)

                                                   atTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCa                                      >  2:6408542/1‑93 (MQ=255)

                                                    ttGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                                     <  1:320948/93‑1 (MQ=255)

                                                    ttGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                                     >  2:4102753/1‑93 (MQ=255)

                                                    ttGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                                     >  2:7826374/1‑93 (MQ=255)

                                                    ttGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                                     <  1:5197582/93‑1 (MQ=255)

                                                      gatgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTg                                   >  1:138399/1‑93 (MQ=255)

                                                      gatgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTg                                   >  2:6567296/1‑93 (MQ=255)

                                                      gatgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTg                                   <  1:6879996/93‑1 (MQ=255)

                                                       atgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTgg                                  >  2:382846/1‑93 (MQ=255)

                                                        tgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGa                                 >  1:7608595/1‑93 (MQ=255)

                                                        tgaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGa                                 <  1:1079432/93‑1 (MQ=255)

                                                         gaCCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAg                                >  1:3030886/1‑93 (MQ=255)

                                                           ccTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCg                              >  2:4967763/1‑93 (MQ=255)

                                                           ccTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCg                              >  2:5697796/1‑93 (MQ=255)

                                                           ccTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCg                              >  2:3367954/1‑93 (MQ=255)

                                                            cTGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGa                             >  1:897499/1‑93 (MQ=255)

                                                             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAt                            >  1:5468447/1‑93 (MQ=255)

                                                              ggCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAtt                           >  2:4780867/1‑93 (MQ=255)

                                                               gCTTATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAttt                          <  1:1857921/93‑1 (MQ=255)

                                                                 ttATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCa                        >  2:3019286/1‑93 (MQ=255)

                                                                 ttATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCa                        <  1:7341060/93‑1 (MQ=255)

                                                                 ttATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCa                        >  2:1349673/1‑93 (MQ=255)

                                                                  tATTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTcct                       <  2:5492896/93‑3 (MQ=255)

                                                                   aTTGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATc                      <  1:3396441/93‑1 (MQ=255)

                                                                    ttGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt                     <  2:5832634/93‑1 (MQ=255)

                                                                     tGCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTg                    >  1:1682981/1‑93 (MQ=255)

                                                                      gCCCTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTgg                   >  2:6198362/1‑93 (MQ=255)

                                                                       cccTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGc                  <  2:546977/93‑1 (MQ=255)

                                                                         cTGGGTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGccc                >  1:3082355/1‑93 (MQ=255)

                                                                          tGGGTGCCACCACCCTCGTCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCt               <  2:7036772/93‑1 (MQ=255)

                                                                           gggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCtt              <  1:4599064/93‑1 (MQ=255)

                                                                            ggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTc             <  1:4364348/93‑1 (MQ=255)

                                                                            ggTGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTc             <  1:674343/93‑1 (MQ=255)

                                                                              tGACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGc           <  1:5909868/93‑1 (MQ=255)

                                                                               gACACCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCa          >  2:1446179/1‑93 (MQ=255)

                                                                                acacCACCCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGCGCGATTTCATCTGGCCCTTCGCAc         >  1:1917136/1‑93 (MQ=255)

                                                                                  cccacccacATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActa       <  2:4435702/92‑1 (MQ=255)

                                                                                  accaccCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActa       >  2:843082/1‑93 (MQ=255)

                                                                                   ccacccacATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActac      <  2:7217591/93‑1 (MQ=255)

                                                                                    caccCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacc     >  2:7872927/1‑93 (MQ=255)

                                                                                     accCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacct    >  1:7651734/1‑93 (MQ=255)

                                                                                     accCACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActacct    >  1:5620744/1‑93 (MQ=255)

                                                                                      cccACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCActaccta   >  1:360687/1‑93 (MQ=255)

                                                                                       ccACATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTAATCTGGCCCTTCGCActacctac  >  2:5345043/1‑93 (MQ=255)

TATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTGGCCCTTCGCACTACCTAC  >  NC_000913/2463920‑2464099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`