BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,470,983	T→C	Y95C (TAC→TGC)	yfdO ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,470,983	0	T	C	99.6%	700.8 / NA	221	Y95C (TAC→TGC)	yfdO	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (110/110); minor base G (1/0); total (111/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CATGCTCACCCCCTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGTACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCACCAGAAC  >  NC_000913/2470893‑2471072

cATGCTCACCCCCTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCAc                                                                                         >  1:4974862/1‑93 (MQ=255)

  tGCTCACCCCCTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGt                                                                                       <  1:466168/93‑1 (MQ=255)

    cTCACCCCCTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGc                                                                                     <  1:1095329/93‑1 (MQ=255)

      cACCCCCTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTg                                                                                   >  1:4376682/1‑93 (MQ=255)

         ccccTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacg                                                                                >  2:5409910/1‑93 (MQ=255)

          cccTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacgg                                                                               >  2:676400/1‑93 (MQ=255)

          cccTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacgg                                                                               <  2:5965069/93‑1 (MQ=255)

           ccTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacgga                                                                              <  2:1672304/93‑1 (MQ=255)

           ccTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacgga                                                                              >  1:6877290/1‑93 (MQ=255)

            cTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAGCTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacggac                                                                             <  1:3388803/93‑1 (MQ=255)

            cTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacggac                                                                             <  1:7361473/93‑1 (MQ=255)

            cTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacggac                                                                             >  1:4214023/1‑93 (MQ=255)

             tGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacggacg                                                                            >  1:1466711/1‑93 (MQ=255)

             tGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTgacggacg                                                                            >  2:3800626/1‑93 (MQ=255)

               aaTCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGc                                                                          <  1:3124622/93‑1 (MQ=255)

                aTCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCg                                                                         >  2:7380851/1‑93 (MQ=255)

                 tCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGa                                                                        >  1:2074379/1‑93 (MQ=255)

                 tCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGa                                                                        <  1:5366235/93‑1 (MQ=255)

                 tCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGa                                                                        >  1:3868094/1‑93 (MQ=255)

                 tCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGa                                                                        <  2:4481081/93‑1 (MQ=255)

                 tCCTGCCGGGATCTGGCTGTAGTCCACAGTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGa                                                                        <  2:3106341/93‑1 (MQ=255)

                   cTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATa                                                                      >  1:3942958/1‑93 (MQ=255)

                    tGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGCAGTACGGGTGTTCGCGTTTTTCTGTGCACGTGCTGACGGAGGGCGATaa                                                                     >  1:862430/1‑93 (MQ=255)

                    tGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATaa                                                                     <  1:4538789/93‑1 (MQ=255)

                    tGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATaa                                                                     >  2:1488053/1‑93 (MQ=255)

                    tGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATaa                                                                     <  1:2034049/93‑1 (MQ=255)

                     gCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAg                                                                    <  1:2232679/93‑1 (MQ=255)

                     gCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAg                                                                    <  1:6581611/93‑1 (MQ=255)

                     gCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAg                                                                    <  1:3603520/93‑1 (MQ=255)

                      gcGGGATCTGGCTGTAGTCCACATTGTTGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAgc                                                                   <  1:1036458/92‑1 (MQ=255)

                        gggATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAgcgc                                                                 >  2:2163714/1‑93 (MQ=255)

                        gggATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAgcgc                                                                 >  1:2504159/1‑93 (MQ=255)

                         ggATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCa                                                                <  2:3364670/93‑1 (MQ=255)

                         ggATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCa                                                                >  2:248965/1‑93 (MQ=255)

                         ggATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCa                                                                <  1:6073837/93‑1 (MQ=255)

                         ggATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCa                                                                >  1:1833951/1‑93 (MQ=255)

                          gATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAg                                                               <  1:7067970/93‑1 (MQ=255)

                           aTCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAgg                                                              >  2:6855766/1‑93 (MQ=255)

                           aTCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAgg                                                              <  1:1915566/93‑1 (MQ=255)

                           aTCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGATGACGGACGGCGATAAGCGCAgg                                                              >  2:4306556/1‑93 (MQ=255)

                            tCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAggg                                                             >  1:778192/1‑93 (MQ=255)

                            tCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAggg                                                             >  1:2138412/1‑93 (MQ=255)

                              tGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGaa                                                           >  2:5007509/1‑93 (MQ=255)

                               ggCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGaaa                                                          <  1:7100208/93‑1 (MQ=255)

                                 cTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGc                                                        <  2:4217747/93‑1 (MQ=255)

                                  tGTAGTCCACATTGTCGTAACTGGCTTTGAAGTAGGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATGAGCGCAGGGAAAGCt                                                       >  1:4828592/1‑93 (MQ=255)

                                  tGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCt                                                       <  2:1789346/93‑1 (MQ=255)

                                   gTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTc                                                      >  2:5461279/1‑93 (MQ=255)

                                      gTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATc                                                   >  2:2114003/1‑93 (MQ=255)

                                       tCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGCAAGCTCATcc                                                  >  2:2880251/1‑93 (MQ=255)

                                       tCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATcc                                                  >  2:359572/1‑93 (MQ=255)

                                       tCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATcc                                                  >  1:3037904/1‑93 (MQ=255)

                                       tCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATcc                                                  >  2:4005204/1‑93 (MQ=255)

                                         cacaTTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATcccg                                                >  2:1238577/1‑92 (MQ=255)

                                         cacaTTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCa                                                >  2:3706625/1‑93 (MQ=255)

                                         cacaTTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCa                                                <  1:1199573/93‑1 (MQ=255)

                                           caTTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCAtt                                              >  1:2953379/1‑93 (MQ=255)

                                                tCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTcc                                         <  2:2436466/93‑1 (MQ=255)

                                                   tAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGc                                      <  1:7291658/93‑1 (MQ=255)

                                                   tAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGc                                      <  2:1168405/93‑1 (MQ=255)

                                                      cTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGc                                   <  1:5507084/93‑1 (MQ=255)

                                                       tGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCt                                  >  2:6343293/1‑93 (MQ=255)

                                                        ggCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCtt                                 <  2:5369236/93‑1 (MQ=255)

                                                        ggCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCtt                                 >  1:6678735/1‑93 (MQ=255)

                                                        ggCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCtt                                 <  2:4752108/93‑1 (MQ=255)

                                                         gCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTc                                <  1:5038740/93‑1 (MQ=255)

                                                         gCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTc                                >  1:1574808/1‑93 (MQ=255)

                                                          cTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCg                               <  2:4655648/93‑1 (MQ=255)

                                                           tttGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGa                              <  2:6502768/93‑1 (MQ=255)

                                                            ttGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGAc                             <  1:2703079/93‑1 (MQ=255)

                                                            ttGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGAc                             <  1:5133022/93‑1 (MQ=255)

                                                            ttGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGAc                             <  1:1263699/93‑1 (MQ=255)

                                                            ttGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGAc                             >  2:504097/1‑93 (MQ=255)

                                                             tGAAGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACg                            <  2:3973547/93‑1 (MQ=255)

                                                               aaGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGTGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACggg                          >  1:4719349/1‑93 (MQ=255)

                                                                aGTACGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGc                         >  2:3528790/1‑93 (MQ=255)

                                                                   aCGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGa                      <  2:7504243/93‑1 (MQ=255)

                                                                    cGGGTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAg                     <  1:4015320/93‑1 (MQ=255)

                                                                      ggTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCa                   <  2:3042948/93‑1 (MQ=255)

                                                                      ggTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCa                   >  1:7548610/1‑93 (MQ=255)

                                                                       gTCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCAc                  >  1:4908346/1‑93 (MQ=255)

                                                                        tCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGTCTGAGCAct                 >  1:4509519/1‑92 (MQ=255)

                                                                        tCTTCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACg                 >  1:368902/1‑93 (MQ=255)

                                                                          ttCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGtt               >  2:2671554/1‑93 (MQ=255)

                                                                          ttCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGtt               >  2:2011375/1‑93 (MQ=255)

                                                                          ttCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGtt               >  1:7245983/1‑93 (MQ=255)

                                                                          ttCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCCTCGACGGGCTGAGCACGtt               >  2:7013814/1‑93 (MQ=255)

                                                                           tCGCGTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTa              >  1:986451/1‑93 (MQ=255)

                                                                            cgcgTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTAc             <  2:2068202/93‑1 (MQ=255)

                                                                            cgcgTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTAc             >  1:1446228/1‑93 (MQ=255)

                                                                             gcgTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACg            <  1:5722326/93‑1 (MQ=255)

                                                                              cgTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGTGCACGTTACgg           <  2:1278320/93‑1 (MQ=255)

                                                                              cgTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACgg           >  2:2313336/1‑93 (MQ=255)

                                                                               gTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGc          >  2:4106837/1‑93 (MQ=255)

                                                                               gTTTTTCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGc          <  1:4347017/93‑1 (MQ=255)

                                                                                tttttCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCa         >  1:2298295/1‑93 (MQ=255)

                                                                                tttttCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCa         >  1:4936232/1‑93 (MQ=255)

                                                                                    tCTGTGCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCACCAg     <  1:5819985/93‑1 (MQ=255)

                                                                                      tgtgCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCACCAGaa   <  2:6051038/93‑1 (MQ=255)

                                                                                      tgtgCACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCACCAGaa   <  2:7934969/93‑1 (MQ=255)

CATGCTCACCCCCTGAATCCTGCCGGGATCTGGCTGTAGTCCACATTGTCGTAACTGGCTTTGAAGTACGGGTCTTCGCGTTTTTCTGTGTACGTGCTGACGGACGGCGATAAGCGCAGGGAAAGCTCATCCCATTTTTCCCGCAGCTTCGACGGGCTGAGCACGTTACGGCACCAGAAC  >  NC_000913/2470893‑2471072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`