BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,917	A→G	L41P (CTG→CCG)	afuC ←	AfuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,917	0	A	G	99.7%	910.6 / ‑5.2	294	L41P (CTG→CCG)	afuC	AfuC
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (140/153); total (140/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 320 total aligned reads displayed.

CCATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCA  >  NC_000913/277827‑278006

ccATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGagcggc                                                                                         >  1:352026/1‑93 (MQ=255)

 cATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAgcggcg                                                                                        <  1:1551888/93‑1 (MQ=255)

  aTCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCACCCAGGCGCACACTAGTGGTTTTGCCGCAGCCGGCCGGGCCGAGCGGCGt                                                                                       >  1:3438251/1‑93 (MQ=255)

  aTCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAgcggcgc                                                                                       >  2:2831785/1‑92 (MQ=255)

  aTCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGt                                                                                       <  1:7280450/93‑1 (MQ=255)

   tCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGTCGTTCGGGCCGAGCGGCGTc                                                                                      >  1:7029632/1‑93 (MQ=255)

   tCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTc                                                                                      >  2:3049247/1‑93 (MQ=255)

    cAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCa                                                                                     >  2:4474224/1‑93 (MQ=255)

    cAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCa                                                                                     <  1:3256481/93‑1 (MQ=255)

          aaaTTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCAttt                                                                               <  2:6226047/93‑1 (MQ=255)

          aaaTTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCAttt                                                                               <  1:429336/93‑1 (MQ=255)

           aaTTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTg                                                                              >  2:1782112/1‑93 (MQ=255)

           aaTTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTg                                                                              >  1:3496316/1‑93 (MQ=255)

             tttGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGcc                                                                            >  1:6689872/1‑93 (MQ=255)

             tttGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGACGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGcc                                                                            >  1:2791779/1‑93 (MQ=255)

              ttGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGccc                                                                           >  1:3756814/1‑93 (MQ=255)

                gCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATGGTGGTTTTTCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCt                                                                         <  1:2171120/93‑1 (MQ=39)

                gCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCt                                                                         >  1:4071644/1‑93 (MQ=255)

                  ccTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGc                                                                       >  2:5063621/1‑93 (MQ=255)

                  ccTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGc                                                                       <  1:1943280/93‑1 (MQ=255)

                   cTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCg                                                                      >  2:4045554/1‑93 (MQ=255)

                    ttCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCgg                                                                     >  1:3002823/1‑93 (MQ=255)

                    ttCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCgg                                                                     >  2:2407528/1‑93 (MQ=255)

                    ttCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCgg                                                                     >  1:2512753/1‑93 (MQ=255)

                      cGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGa                                                                   <  1:7376281/93‑1 (MQ=255)

                      cGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGa                                                                   >  1:939799/1‑93 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  >  2:3669547/1‑93 (MQ=255)

                       gCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGAt                                                                  <  1:580083/93‑1 (MQ=255)

                        cTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCCGCGGGATg                                                                 >  2:7040698/1‑93 (MQ=255)

                         tCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATgg                                                                <  2:1195555/93‑1 (MQ=255)

                            gTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGa                                                             <  1:6252377/93‑1 (MQ=255)

                             gttttCGAGCGGGGCAACCAGGGGCAAAATAGTGGTTTTGCCGCGTCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAg                                                            <  2:6928823/92‑1 (MQ=255)

                              ttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGatg                                                           <  2:4676638/93‑3 (MQ=255)

                              ttttCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAgg                                                           >  1:2703375/1‑93 (MQ=255)

                                 tCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTg                                                        >  1:204442/1‑93 (MQ=255)

                                  ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGa                                                       >  2:7914437/1‑93 (MQ=255)

                                  ccAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGa                                                       <  2:524935/93‑1 (MQ=255)

                                   cAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCGGCAGCCGGACGGTCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGat                                                      <  1:5686907/93‑1 (MQ=255)

                                    aGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGata                                                     <  1:3318760/93‑1 (MQ=255)

                                    aGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGata                                                     >  2:1417685/1‑93 (MQ=255)

                                     gCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGatat                                                    >  1:7883650/1‑93 (MQ=255)

                                       ccGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTg                                                  <  2:3421342/93‑1 (MQ=255)

                                        cGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGt                                                 <  2:3807490/93‑1 (MQ=255)

                                        cGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGt                                                 <  1:4462894/93‑1 (MQ=255)

                                         ggCAACCAGTCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTc                                                <  2:1224933/93‑1 (MQ=255)

                                          gCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCg                                               >  2:1961700/1‑93 (MQ=255)

                                           cAACCAGGTGCAAGATATTGGTTTGCCCGCGGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGa                                              <  1:1741686/93‑1 (MQ=255)

                                           cAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGa                                              <  2:6682364/93‑1 (MQ=255)

                                           cAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGa                                              <  2:7380009/93‑1 (MQ=255)

                                           cAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGa                                              <  2:2256424/93‑1 (MQ=255)

                                             aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGAtt                                            >  1:1640567/1‑93 (MQ=255)

                                             aCCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGAtt                                            <  1:7314190/93‑1 (MQ=255)

                                              ccAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTa                                           <  2:5447071/93‑1 (MQ=255)

                                                aGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTAcc                                         >  2:7861237/1‑93 (MQ=255)

                                                 ggCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCg                                        >  2:2182251/1‑93 (MQ=255)

                                                   ggcAAAATAGTGGTTTTGCCGCAGCCGGAGGGGCCGGGCGGCGTCACCATTTGCCCCTGGGGGATGGTGAGGTTGATATTGTCGATTACCGTa                                      <  2:6459670/92‑1 (MQ=255)

                                                     cAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTCATATTGTCGATTACCGTAtt                                    <  2:7616199/93‑1 (MQ=255)

                                                       aaaTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTAc                                  >  2:1643076/1‑93 (MQ=255)

                                                         aTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTg                                <  1:2367009/93‑1 (MQ=255)

                                                         aTAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTg                                <  1:5048166/93‑1 (MQ=255)

                                                          tAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGc                               <  1:4602156/93‑1 (MQ=255)

                                                            gTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCa                             >  1:5543760/1‑93 (MQ=255)

                                                            gTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCa                             >  1:7471630/1‑93 (MQ=255)

                                                            gTGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCa                             >  2:4396412/1‑93 (MQ=255)

                                                             tGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCaa                            <  1:1051365/93‑1 (MQ=255)

                                                             tGGTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCaa                            >  1:4703793/1‑93 (MQ=255)

                                                              ggTTTTGCCGCAGCCGGACGGGCTGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCaaa                           <  2:4278395/93‑1 (MQ=255)

                                                              ggTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCaaa                           <  2:5628808/93‑1 (MQ=255)

                                                              ggTTTTGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCaaa                           >  2:6333572/1‑93 (MQ=255)

                                                                  ttGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGt                       <  2:2607824/93‑1 (MQ=255)

                                                                  ttGCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGt                       <  1:4390304/93‑1 (MQ=255)

                                                                    gCCGCAGCCGGACGGGCCGCGCGGCGTCACCATTTGCCCCAGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTTCTGCCAAATCGttt                     <  2:3459355/93‑1 (MQ=255)

                                                                    gCCGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGttt                     >  2:4665174/1‑93 (MQ=255)

                                                                     ccGCAGCCGGACGGTCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    <  1:4811167/93‑1 (MQ=255)

                                                                     ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    <  2:6722389/93‑1 (MQ=255)

                                                                     ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    <  2:5349662/93‑1 (MQ=255)

                                                                     ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    <  2:5861029/93‑1 (MQ=255)

                                                                     ccGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCCGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTa                    >  2:770545/1‑93 (MQ=255)

                                                                      cGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                   <  1:5709250/93‑1 (MQ=255)

                                                                      cGCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAg                   <  1:5136557/93‑1 (MQ=255)

                                                                       gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGGTGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGt                  >  2:6105239/1‑93 (MQ=255)

                                                                       gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGt                  <  2:482597/93‑1 (MQ=255)

                                                                       gCAGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGt                  <  1:5484114/93‑1 (MQ=255)

                                                                         aGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                <  1:6832752/93‑1 (MQ=255)

                                                                         aGCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGa                <  2:3750426/93‑1 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc               >  1:6046619/1‑93 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc               >  1:6432475/1‑93 (MQ=255)

                                                                          gCCGGACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGAc               >  2:5777031/1‑93 (MQ=255)

                                                                             ggACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt            <  2:7502091/93‑1 (MQ=255)

                                                                             ggACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt            <  2:2551836/93‑1 (MQ=255)

                                                                             ggACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGtt            >  1:7429364/1‑93 (MQ=255)

                                                                              gACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTg           <  2:6782119/93‑1 (MQ=255)

                                                                              gACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTg           >  2:449347/1‑93 (MQ=255)

                                                                              gACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTg           <  2:7242413/93‑1 (MQ=255)

                                                                              gACGGGCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTg           <  2:3617813/93‑1 (MQ=255)

                                                                                  ggCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCa       >  2:5687765/1‑93 (MQ=255)

                                                                                  ggCCGAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCa       <  1:1259183/93‑1 (MQ=255)

                                                                                      gAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTc   >  2:385134/1‑93 (MQ=255)

                                                                                      gAGCGGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTc   >  2:1866696/1‑93 (MQ=255)

                                                                                       agcggcGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCa  >  1:7589684/1‑93 (MQ=255)

CCATCAATGAAAATTTGCCCTTCGCTCGGTTTTTCCAGCCCGGCAACCAGGCGCAAAATAGTGGTTTTGCCGCAGCCGGACGGGCCGAGCAGCGTCACCATTTGCCCCTGCGGGATGGTGAGGTTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCA  >  NC_000913/277827‑278006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`