BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,381	A→G	I530I (ATT→ATC)	b2373 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,381	0	A	G	99.5%	669.4 / ‑5.2	211	I530I (ATT→ATC)	b2373	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (78/132); total (79/132)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 218 total aligned reads displayed.

TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470

ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         >  1:4767774/1‑93 (MQ=255)

ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  1:4421477/93‑1 (MQ=255)

ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         >  1:6065136/1‑93 (MQ=255)

 tGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATa                                                                                        <  2:1549315/93‑1 (MQ=255)

 tGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATa                                                                                        <  2:2380618/93‑1 (MQ=255)

   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      <  2:7186212/93‑1 (MQ=255)

   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      >  2:7075342/1‑93 (MQ=255)

    tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCg                                                                                     <  2:6717327/93‑1 (MQ=255)

    tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCg                                                                                     <  1:1982789/93‑1 (MQ=255)

     ttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCgg                                                                                    <  1:7466865/93‑1 (MQ=255)

      tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggt                                                                                   <  1:3145061/93‑1 (MQ=255)

       gggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtg                                                                                  >  1:5201698/1‑93 (MQ=255)

       gggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtg                                                                                  <  2:2830190/93‑1 (MQ=255)

        ggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtgg                                                                                 <  1:1684291/93‑1 (MQ=255)

         gTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtggt                                                                                >  2:5654816/1‑93 (MQ=255)

          ttAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGtt                                                                               <  2:3157892/93‑1 (MQ=255)

            aaGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTaa                                                                             <  1:3394448/93‑1 (MQ=255)

                ttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCa                                                                         <  1:6451895/93‑1 (MQ=255)

                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       >  2:1255953/1‑93 (MQ=255)

                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       >  1:5668273/1‑93 (MQ=255)

                   gTAATATGGCTACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGa                                                                      <  2:4515636/93‑1 (MQ=255)

                   gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGa                                                                      <  2:5986615/93‑1 (MQ=255)

                   gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATCACCACATTAATAATGCTCGGTTTGTGCGACTGGACACCGGTGGTTACACCATGa                                                                      >  1:833024/1‑93 (MQ=255)

                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   <  1:6903528/93‑1 (MQ=255)

                        atGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAg                                                                 >  1:6327283/1‑93 (MQ=255)

                          ggCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGtt                                                               <  2:3561908/93‑1 (MQ=255)

                          ggCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGtt                                                               >  1:4584334/1‑93 (MQ=255)

                          ggCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGtt                                                               >  1:4336793/1‑93 (MQ=255)

                            ccACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCa                                                             <  2:6164731/93‑1 (MQ=255)

                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            >  2:1922549/1‑93 (MQ=255)

                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            >  2:7272/1‑93 (MQ=255)

                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  1:7612745/93‑1 (MQ=255)

                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           >  2:4946537/1‑93 (MQ=255)

                              aCTTTCAGTTCCTGCTGCAGGGTCAATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  1:700579/93‑1 (MQ=255)

                               cTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCt                                                          >  1:4904051/1‑93 (MQ=255)

                               cTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCt                                                          <  2:2890834/93‑1 (MQ=255)

                                tttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCtt                                                         >  1:5433868/1‑92 (MQ=255)

                                tttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTg                                                         >  1:1186321/1‑93 (MQ=255)

                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  2:492272/93‑1 (MQ=255)

                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  2:4075416/93‑1 (MQ=255)

                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  1:766217/93‑1 (MQ=255)

                                   cAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCg                                                      <  2:1718855/93‑1 (MQ=255)

                                     gTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtg                                                    >  1:6816884/1‑93 (MQ=255)

                                        ccTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtg                                                 <  1:4858357/93‑1 (MQ=255)

                                         ctgctgCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtgc                                                >  2:3184872/1‑92 (MQ=255)

                                         ctgctgCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGa                                                <  1:3676380/93‑1 (MQ=255)

                                         ctgctgCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGa                                                <  2:3289623/93‑1 (MQ=255)

                                         ctgctgCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtgc                                                >  1:6504236/1‑92 (MQ=255)

                                          tgctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc                                               <  2:3624905/93‑1 (MQ=255)

                                            ctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGt                                             <  2:5746406/93‑1 (MQ=255)

                                            ctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGt                                             >  1:7252209/1‑93 (MQ=255)

                                             tgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGtt                                            <  1:2971411/93‑1 (MQ=255)

                                              gcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTta                                           <  2:3710170/93‑1 (MQ=255)

                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          <  2:867219/93‑1 (MQ=255)

                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          <  1:2026286/93‑1 (MQ=255)

                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          <  1:152197/93‑1 (MQ=255)

                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          >  2:7656615/1‑93 (MQ=255)

                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          <  1:4851436/93‑1 (MQ=255)

                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  2:5900942/93‑1 (MQ=255)

                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  1:5296806/93‑1 (MQ=255)

                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  1:6205442/93‑1 (MQ=255)

                                                 gggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtatac                                        >  1:3134066/1‑92 (MQ=255)

                                                  ggTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGc                                       <  1:4607419/93‑1 (MQ=255)

                                                    tCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCa                                     >  1:7174050/1‑93 (MQ=255)

                                                      gATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAc                                   >  1:6346932/1‑93 (MQ=255)

                                                      gATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAc                                   >  1:2701105/1‑93 (MQ=255)

                                                        tGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGc                                 >  2:5412428/1‑93 (MQ=255)

                                                          aCCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCa                               <  1:2136734/93‑1 (MQ=255)

                                                          aCCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCa                               <  2:1840068/93‑1 (MQ=255)

                                                             acaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGa                            <  2:6071437/93‑1 (MQ=255)

                                                             acaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGa                            <  2:5130452/93‑1 (MQ=255)

                                                             acaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGa                            >  2:7729334/1‑93 (MQ=255)

                                                               aTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGaaa                          <  2:20785/93‑1 (MQ=255)

                                                               aTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGaaa                          <  2:6306464/93‑1 (MQ=255)

                                                                ttAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAc                         <  2:4393114/93‑1 (MQ=255)

                                                                  aataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGc                       <  1:3605321/93‑1 (MQ=255)

                                                                  aataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGc                       >  1:6970462/1‑93 (MQ=255)

                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      >  1:4022531/1‑93 (MQ=255)

                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  1:6629108/93‑1 (MQ=255)

                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  1:1053282/93‑1 (MQ=255)

                                                                     aatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATc                    <  2:7034166/93‑1 (MQ=255)

                                                                     aatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATc                    <  2:4421271/93‑1 (MQ=255)

                                                                     aatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATc                    <  1:385538/93‑1 (MQ=255)

                                                                      atGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATcc                   >  2:1037797/1‑93 (MQ=255)

                                                                       tGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  2:4557250/93‑1 (MQ=255)

                                                                        ggTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGTATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAt                 <  2:1789728/93‑1 (MQ=255)

                                                                           cGGTTTGCGCGACTGGATACCGGTGGTTGAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  1:2472448/93‑1 (MQ=255)

                                                                           cGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  2:5153662/93‑1 (MQ=255)

                                                                           cGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  1:4249447/93‑1 (MQ=255)

                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             <  1:229240/93‑1 (MQ=255)

                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             >  1:1801532/1‑93 (MQ=255)

                                                                             gtgtGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            <  2:2207823/90‑1 (MQ=255)

                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            >  1:1408733/1‑93 (MQ=255)

                                                                              tttGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAttt           <  1:5977662/93‑1 (MQ=255)

                                                                                tGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGt         <  2:4657280/93‑1 (MQ=255)

                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  1:2564462/93‑1 (MQ=255)

                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  2:2419884/93‑1 (MQ=255)

                                                                                   gcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAt      <  1:817418/93‑1 (MQ=255)

                                                                                    cgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATa     <  1:6785031/93‑1 (MQ=255)

                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   >  2:7408118/1‑93 (MQ=255)

TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`