BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,993	A→G	D303G (GAC→GGC)	ptsI →	PEP‑protein phosphotransferase system enzyme I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,993	0	A	G	100.0%	907.3 / NA	287	D303G (GAC→GGC)	ptsI	PEP‑protein phosphotransferase system enzyme I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (127/160); total (127/160)

Only 100 of 290 total aligned reads displayed.

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

    cATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGa                                                                                     <  1:3556674/93‑1 (MQ=255)

     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    <  2:5656497/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAATGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:7072070/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  1:902220/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:7928874/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:1540451/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  1:742179/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  1:5645566/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  1:5142137/93‑1 (MQ=255)

          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               >  1:2177279/1‑93 (MQ=255)

          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               <  2:3635069/93‑1 (MQ=255)

          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCCCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               <  2:2979602/93‑1 (MQ=255)

            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             <  1:4934480/93‑1 (MQ=255)

             ggTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGcc                                                                            >  1:6285209/1‑93 (MQ=255)

              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           >  2:4945044/1‑93 (MQ=255)

              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           >  2:1447072/1‑93 (MQ=255)

                 cGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACt                                                                        >  1:1743215/1‑93 (MQ=255)

                 cGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACt                                                                        >  1:4232058/1‑93 (MQ=255)

                 cGTGACGTTGAAGGTGCAGAGCGGAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTCCCTGTTCATGGGCCGCGACGCACTGCCCACt                                                                        <  2:5408228/93‑1 (MQ=255)

                 cGTGACGTGGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACt                                                                        <  2:5566091/93‑1 (MQ=255)

                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       >  2:6854238/1‑93 (MQ=255)

                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       >  1:1859059/1‑93 (MQ=255)

                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       >  1:3476710/1‑93 (MQ=255)

                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      <  2:107976/93‑1 (MQ=255)

                    gACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     <  1:305920/93‑1 (MQ=255)

                    gACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     <  1:6404087/93‑1 (MQ=255)

                    gACGTTCAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     <  1:6660572/93‑1 (MQ=255)

                     aCGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaag                                                                    >  1:3651449/1‑93 (MQ=255)

                       gTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaa                                                                  <  2:2033447/93‑1 (MQ=255)

                       gTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaa                                                                  >  2:1799481/1‑93 (MQ=255)

                       gTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaa                                                                  >  1:6101125/1‑93 (MQ=255)

                         tGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaaga                                                                <  2:4043840/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               <  2:3907351/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               <  2:5234397/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               >  1:2491997/1‑93 (MQ=255)

                             ggTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            <  1:453424/93‑1 (MQ=255)

                               tGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTCAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  1:7042705/93‑1 (MQ=255)

                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  1:4963597/93‑1 (MQ=255)

                                    agCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgctg                                                     <  1:7302668/93‑1 (MQ=255)

                                       gTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCtt                                                  >  1:1184830/1‑93 (MQ=255)

                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 >  1:6559775/1‑93 (MQ=255)

                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 <  2:6969343/93‑1 (MQ=255)

                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 <  2:2361025/93‑1 (MQ=255)

                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               >  2:6606096/1‑93 (MQ=255)

                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               >  1:3358519/1‑93 (MQ=255)

                                            ggCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaaa                                             <  1:618791/93‑1 (MQ=255)

                                            ggCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaaa                                             <  2:5813147/93‑1 (MQ=255)

                                            ggCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaaa                                             <  1:1416734/93‑1 (MQ=255)

                                             gcgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAg                                            >  2:6487614/1‑93 (MQ=255)

                                              cgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGc                                           <  1:5892101/93‑1 (MQ=255)

                                              cgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGc                                           <  2:4741717/93‑1 (MQ=255)

                                                cTGAAGGTGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         >  2:4506941/1‑93 (MQ=255)

                                                cTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         <  2:6232752/93‑1 (MQ=255)

                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  1:5792292/93‑1 (MQ=255)

                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  1:2725889/93‑1 (MQ=255)

                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        >  2:3268932/1‑93 (MQ=255)

                                                  taaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  2:4554021/92‑1 (MQ=255)

                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     <  2:7180427/93‑1 (MQ=255)

                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     >  2:2476160/1‑93 (MQ=255)

                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    >  2:3418173/1‑93 (MQ=255)

                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    <  2:4073051/93‑1 (MQ=255)

                                                       cGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGa                                  >  2:5112588/1‑93 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 <  2:7691261/93‑1 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 >  2:2636822/1‑93 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 >  1:5989692/1‑93 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 >  1:4721291/1‑93 (MQ=255)

                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                <  2:1699138/93‑1 (MQ=255)

                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                >  2:7539490/1‑93 (MQ=255)

                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                >  1:4840096/1‑93 (MQ=255)

                                                          ttgTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGGGGCTGAAGc                               <  1:5232991/91‑1 (MQ=255)

                                                          tGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGc                               <  1:4507078/93‑1 (MQ=255)

                                                          tGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGc                               >  1:7892911/1‑93 (MQ=255)

                                                           ggTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCg                              <  2:2281112/93‑1 (MQ=255)

                                                            gTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgt                             >  1:6507755/1‑93 (MQ=255)

                                                            gTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgt                             <  2:6149226/93‑1 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            >  2:7298935/1‑93 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            <  1:4052721/93‑1 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCACACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            <  1:1114018/93‑1 (MQ=255)

                                                              cTGTCTCGTACTGAGTTCCTGTTCATGGGACGCGACGGACTGCCGACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           <  1:2527037/93‑1 (MQ=255)

                                                              cTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           <  1:2078373/93‑1 (MQ=255)

                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          >  1:3836323/1‑93 (MQ=255)

                                                                gTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTgg                         >  2:5702353/1‑93 (MQ=255)

                                                                 tATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGc                        >  1:323243/1‑93 (MQ=255)

                                                                      tACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCa                   >  1:3942/1‑93 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 >  1:1607727/1‑93 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  1:5396282/93‑1 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGTGACGAATTGCCTACTGAAGAAGACCAATTTGCTGATTACAAAGCATTGGCTGAAGCGTGTGGCTCGCAAGc                <  2:2468963/93‑1 (MQ=255)

                                                                          gAGTTCCTGTTCATGGGCCGCGACGCACTGTCCACTGAAGAAGCACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCAAAAGCg               >  1:4587505/1‑93 (MQ=255)

                                                                             ttCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGtt            >  2:1281013/1‑93 (MQ=255)

                                                                              tCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGGTAGCgggtt           >  2:3381564/1‑90 (MQ=255)

                                                                              tCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTa           <  2:3187649/93‑1 (MQ=255)

                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  2:7871755/93‑1 (MQ=255)

                                                                                cTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtc         >  1:2409732/1‑93 (MQ=255)

                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcg        <  1:5322020/93‑1 (MQ=255)

                                                                                   ttCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgtt      <  1:4813529/93‑1 (MQ=255)

                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     <  2:3830227/93‑1 (MQ=255)

                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     >  2:5868550/1‑93 (MQ=255)

                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     >  1:2277160/1‑93 (MQ=255)

                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTa  >  2:5195601/1‑93 (MQ=255)

                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGCTATCGTTCGTa  >  2:3358221/1‑93 (MQ=255)

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`