BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,649	T→C	C409R (TGC→CGC)	yagF →	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,649	0	T	C	99.6%	763.0 / ‑5.2	247	C409R (TGC→CGC)	yagF	putative dehydratase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (111/135); total (112/135)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 259 total aligned reads displayed.

ATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGC  >  NC_000913/283560‑283738

aTCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGcc                                                                                        >  2:4582546/1‑93 (MQ=255)

   tGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgc                                                                                     >  2:732327/1‑93 (MQ=255)

     gACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgcgc                                                                                   >  1:3393208/1‑93 (MQ=255)

     gACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgcgc                                                                                   <  1:2419295/93‑1 (MQ=255)

     gACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTgcgc                                                                                   <  1:4029910/93‑1 (MQ=255)

        gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAg                                                                                >  2:4956876/1‑93 (MQ=255)

        gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAg                                                                                <  2:225776/93‑1 (MQ=255)

        gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAg                                                                                >  1:524403/1‑93 (MQ=255)

         ccATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGc                                                                               <  2:4390128/93‑1 (MQ=255)

          cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCa                                                                              <  2:3484540/93‑1 (MQ=255)

          cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCa                                                                              <  1:3699924/93‑1 (MQ=255)

           aTGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAg                                                                             >  1:5175693/1‑93 (MQ=255)

            tgaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGGGCAgg                                                                            >  1:1280917/1‑93 (MQ=255)

            tgaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAgg                                                                            >  2:2601841/1‑93 (MQ=255)

                cgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACgg                                                                        >  1:1446143/1‑93 (MQ=255)

                 gtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGc                                                                       <  2:5510053/93‑1 (MQ=255)

                 gtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGc                                                                       >  2:6620078/1‑93 (MQ=255)

                    cccgGCAAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGTGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTa                                                                    <  1:6121866/92‑1 (MQ=255)

                     ccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTag                                                                   <  1:2814336/93‑1 (MQ=255)

                     ccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTag                                                                   >  1:1072265/1‑93 (MQ=255)

                      cgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTaga                                                                  <  1:6934274/93‑1 (MQ=255)

                       ggCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTagag                                                                 <  1:6510068/93‑1 (MQ=255)

                       ggCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTagag                                                                 <  1:2299753/93‑1 (MQ=255)

                        gCCAGACGGTGGGCGAGAACCTTGAATGGTGGCACGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGc                                                                >  2:917187/1‑93 (MQ=255)

                         ccAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGcc                                                               >  2:5655418/1‑93 (MQ=255)

                          cAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCg                                                              >  1:1871258/1‑93 (MQ=255)

                            gACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGa                                                            <  2:4907139/93‑1 (MQ=255)

                            gACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGa                                                            <  2:7895414/93‑1 (MQ=255)

                            gACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGa                                                            >  2:7500804/1‑93 (MQ=255)

                             aCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAt                                                           <  1:4812172/93‑1 (MQ=255)

                             aCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAt                                                           >  2:7200919/1‑93 (MQ=255)

                             aCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAt                                                           <  1:1355248/93‑1 (MQ=255)

                              cGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGGTg                                                          >  2:1612142/1‑93 (MQ=255)

                              cGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATg                                                          >  2:4479332/1‑93 (MQ=255)

                               ggTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGa                                                         <  1:1468936/93‑1 (MQ=255)

                               cgTGGCCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGa                                                         <  1:3156856/92‑1 (MQ=255)

                                   ggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTg                                                     <  1:4600584/93‑1 (MQ=255)

                                   ggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTg                                                     >  2:1389968/1‑93 (MQ=255)

                                    gCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGa                                                    <  1:4544160/93‑1 (MQ=255)

                                     cGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGAt                                                   <  1:5065672/93‑1 (MQ=255)

                                     cGAGAACCTTGAATGGTGGCAGGAGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGAt                                                   >  2:588675/1‑93 (MQ=255)

                                      gagaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATc                                                  <  2:3622730/93‑1 (MQ=255)

                                      gagaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATc                                                  <  2:6268638/93‑1 (MQ=255)

                                       agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc                                                 <  1:1156226/93‑1 (MQ=255)

                                       agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc                                                 >  2:1870256/1‑93 (MQ=255)

                                       agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc                                                 >  2:3361562/1‑93 (MQ=255)

                                       agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATcc                                                 <  1:5353053/93‑1 (MQ=255)

                                        gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCt                                                <  1:5555040/93‑1 (MQ=255)

                                         aaCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTg                                               >  1:565161/1‑93 (MQ=255)

                                         aaCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTACAGCCGGATGACGTGATCCTg                                               >  1:189147/1‑93 (MQ=255)

                                          aCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgc                                              >  2:4195400/1‑93 (MQ=255)

                                            cTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccg                                            >  2:1978408/1‑93 (MQ=255)

                                             ttGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTgccgc                                           >  2:2333558/1‑93 (MQ=255)

                                                 aTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCggg                                       >  2:3543217/1‑92 (MQ=255)

                                                 aTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGga                                       <  2:3780108/93‑1 (MQ=255)

                                                 aTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGga                                       <  1:869304/93‑1 (MQ=255)

                                                     tggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAg                                   <  1:6047350/93‑1 (MQ=255)

                                                       gCAGGCGTCGGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGc                                 <  1:597919/93‑1 (MQ=255)

                                                       gCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGc                                 >  2:5118891/1‑93 (MQ=255)

                                                        cAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCa                                <  2:5199670/93‑1 (MQ=255)

                                                         aGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaa                               >  2:3049280/1‑93 (MQ=255)

                                                          ggCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaa                              >  1:7144926/1‑93 (MQ=255)

                                                           gCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaaa                             <  1:2501403/93‑1 (MQ=255)

                                                           gCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaaa                             <  1:5525470/93‑1 (MQ=255)

                                                           gCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaaa                             <  1:720656/93‑1 (MQ=255)

                                                            cgggcGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAg                            <  1:3065885/89‑1 (MQ=255)

                                                             gTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGc                           >  1:6376910/1‑93 (MQ=255)

                                                               ccGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGa                         <  2:7241074/93‑1 (MQ=255)

                                                                cGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaa                        >  2:1726027/1‑93 (MQ=255)

                                                                cGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaa                        >  1:7841140/1‑93 (MQ=255)

                                                                 gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCTAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       <  1:7631447/93‑1 (MQ=255)

                                                                 gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       <  1:6095121/93‑1 (MQ=255)

                                                                 gAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                       <  2:6823019/93‑1 (MQ=255)

                                                                   gcgcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAgg                     <  2:1434030/93‑1 (MQ=255)

                                                                   gcgcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAgg                     >  2:2960138/1‑93 (MQ=255)

                                                                     gcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGc                   <  2:1256803/93‑1 (MQ=255)

                                                                     gcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGc                   <  2:4017137/93‑1 (MQ=255)

                                                                     gcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGc                   <  1:2155546/93‑1 (MQ=255)

                                                                     gcCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGc                   <  1:1939221/93‑1 (MQ=255)

                                                                      ccGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCt                  <  2:7599304/93‑1 (MQ=255)

                                                                      ccGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCt                  <  1:6378805/93‑1 (MQ=255)

                                                                        gggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGa                >  2:7927091/1‑93 (MQ=255)

                                                                        gggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGa                <  2:5774724/93‑1 (MQ=255)

                                                                         ggCGCGCTTCCGCCAGCTCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAc               <  1:4524346/93‑1 (MQ=255)

                                                                         ggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAGGGCAAAAGCGAAAGGAGTGAc               >  2:6223130/1‑93 (MQ=255)

                                                                         ggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAc               <  1:2473323/93‑1 (MQ=255)

                                                                         ggCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAc               <  2:1261180/93‑1 (MQ=255)

                                                                           cgcgcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCt             >  1:2684387/1‑93 (MQ=255)

                                                                             cgcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGTCAAAAGCGAAAGGGCTGACCTCg           >  2:4287764/1‑93 (MQ=255)

                                                                              gcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGa          >  2:5948228/1‑93 (MQ=255)

                                                                              gcTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGa          <  1:361149/93‑1 (MQ=255)

                                                                               cTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAc         >  2:2833860/1‑93 (MQ=255)

                                                                               cTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAc         <  1:172617/93‑1 (MQ=255)

                                                                                ttCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACg        <  1:3498026/93‑1 (MQ=255)

                                                                                ttCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACg        <  1:3580061/93‑1 (MQ=255)

                                                                                   cGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTc     <  2:644397/93‑1 (MQ=255)

                                                                                    gCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCt    <  1:7530084/93‑1 (MQ=255)

                                                                                     ccAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTg   >  1:367230/1‑93 (MQ=255)

                                                                                      cAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGc  >  1:3679502/1‑93 (MQ=255)

                                                                                      cAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGc  <  1:4238883/93‑1 (MQ=255)

ATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGC  >  NC_000913/283560‑283738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`