BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,775	A→G	Y386C (TAC→TGC)	yagG →	putative permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,775	0	A	G	99.6%	720.8 / ‑4.8	236	Y386C (TAC→TGC)	yagG	putative permease
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (111/123); total (111/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 259 total aligned reads displayed.

GCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCT  >  NC_000913/285685‑285864

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         >  1:1704478/1‑93 (MQ=255)

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         >  2:4425578/1‑93 (MQ=255)

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         >  2:380366/1‑93 (MQ=255)

 ccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGgcgtgcgt                                                                                        >  1:6408429/1‑93 (MQ=255)

    cGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCaa                                                                                     <  2:4256251/93‑1 (MQ=255)

      aCGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGGGGGCTGGATCCTGGCGTGCGTCCACt                                                                                   >  1:3237644/1‑93 (MQ=255)

       cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                  <  1:197576/93‑1 (MQ=255)

       cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                  <  2:4831629/93‑1 (MQ=255)

        gggCTGGTGTTCTCCCCCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                 <  1:6047921/93‑1 (MQ=255)

        gggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAt                                                                                 <  2:6558373/93‑1 (MQ=255)

        gggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGGGGGCTGGATCCTGGCGGGCGTCAACTAt                                                                                 >  1:2768983/1‑93 (MQ=255)

         ggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAtt                                                                                <  2:2538662/93‑1 (MQ=255)

          gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                               <  1:3733619/93‑1 (MQ=255)

          gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                               <  1:1199372/93‑1 (MQ=255)

           cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                              <  1:1090533/93‑1 (MQ=255)

           cTGGCGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                              <  2:2720661/93‑1 (MQ=255)

            tGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCg                                                                             >  1:7145496/1‑93 (MQ=255)

             ggTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGc                                                                            >  1:6335118/1‑93 (MQ=255)

              gtgtTCTCCGCCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTTGGCTGGATCCTGGCGTGCGTCAACTATTCCGcc                                                                           >  1:4231954/1‑93 (MQ=255)

              gtgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGcc                                                                           >  2:7457090/1‑93 (MQ=255)

               tgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCca                                                                          >  2:1135862/1‑93 (MQ=255)

                gtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcag                                                                         >  2:3360606/1‑93 (MQ=255)

                  tctcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagca                                                                       <  2:6266208/93‑1 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     >  1:1402403/1‑93 (MQ=255)

                     ccACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagca                                                                    <  1:3072744/93‑1 (MQ=255)

                      cACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagcag                                                                   >  1:3399706/1‑93 (MQ=255)

                        cctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCg                                                                 >  1:5335872/1‑93 (MQ=255)

                         ctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGt                                                                <  1:4956602/93‑1 (MQ=255)

                            cctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCa                                                             <  2:7888283/93‑1 (MQ=255)

                            cctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCa                                                             <  2:399678/93‑1 (MQ=255)

                            cctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCa                                                             >  2:7097/1‑93 (MQ=255)

                             ctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAg                                                            >  2:4995908/1‑93 (MQ=255)

                              tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                           <  2:4045424/93‑1 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          >  1:5984225/1‑93 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          >  1:3489079/1‑93 (MQ=255)

                                ttCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCg                                                         >  2:3456845/1‑93 (MQ=255)

                                ttCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCg                                                         <  2:4157590/93‑1 (MQ=255)

                                 tCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGAAGACgg                                                        >  1:60923/1‑93 (MQ=255)

                                  cAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGTTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGt                                                       <  2:3028103/93‑1 (MQ=255)

                                  cAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGt                                                       <  2:5507699/93‑1 (MQ=255)

                                   aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                      >  1:6626341/1‑93 (MQ=255)

                                   aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                      <  2:2290314/93‑1 (MQ=255)

                                   aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                      <  2:1945893/93‑1 (MQ=255)

                                   aGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGtt                                                      <  2:1618531/93‑1 (MQ=255)

                                    gCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTg                                                     >  2:1777614/1‑93 (MQ=255)

                                     ccTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGa                                                    <  2:3142701/93‑1 (MQ=255)

                                      cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                   <  1:6289136/93‑1 (MQ=255)

                                      cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                   <  2:6566910/93‑1 (MQ=255)

                                      cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGCGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGGTGAg                                                   >  1:4840261/1‑93 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  >  1:4880098/1‑93 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  >  1:6245197/1‑93 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  <  1:867485/93‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  >  1:6641717/1‑93 (MQ=255)

                                        gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGt                                                 <  2:1858330/93‑1 (MQ=255)

                                         aaGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTg                                                >  2:3984219/1‑93 (MQ=255)

                                         aaGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTg                                                <  2:579010/93‑1 (MQ=255)

                                             ttGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTca                                            <  2:5224422/93‑1 (MQ=255)

                                                gCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTcacca                                         <  1:3941307/93‑1 (MQ=255)

                                                  cTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCaccacc                                       <  2:763795/93‑1 (MQ=255)

                                                  cTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCaccacc                                       >  2:6444493/1‑93 (MQ=255)

                                                  cTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGGGCAGCCGGTTGAGGGGCTCaccacc                                       >  1:4062617/1‑93 (MQ=255)

                                                   tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccacca                                      >  1:5447165/1‑93 (MQ=255)

                                                   tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccacca                                      <  2:6861781/93‑1 (MQ=255)

                                                   tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccacca                                      >  2:4741018/1‑93 (MQ=255)

                                                    ggCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCAt                                     <  1:7574221/93‑1 (MQ=255)

                                                      cGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCa                                   <  2:7330883/93‑1 (MQ=255)

                                                       gATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaa                                  <  1:5168340/93‑1 (MQ=255)

                                                       gATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaa                                  <  1:2426468/93‑1 (MQ=255)

                                                       gATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaa                                  >  2:5435530/1‑93 (MQ=255)

                                                        aTTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaaa                                 >  2:3856870/1‑93 (MQ=255)

                                                          tGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAt                               <  2:5122201/93‑1 (MQ=255)

                                                             cggggcggTGGTGGTCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttct                            <  1:3871834/93‑1 (MQ=255)

                                                             cggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttct                            <  2:1469126/93‑1 (MQ=255)

                                                              gggggggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctg                           <  2:5038847/93‑1 (MQ=255)

                                                              ggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctg                           >  2:7214438/1‑93 (MQ=255)

                                                                 gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                        <  1:2631227/93‑1 (MQ=255)

                                                                  cggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttct                       <  2:3559501/93‑1 (MQ=255)

                                                                   ggtggtGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttctg                      <  1:5248480/93‑1 (MQ=255)

                                                                   ggtggtGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttctg                      >  1:3585883/1‑93 (MQ=255)

                                                                    gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                     <  1:1665886/93‑1 (MQ=255)

                                                                    gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                     <  2:4374638/93‑1 (MQ=255)

                                                                    gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                     <  1:296763/93‑1 (MQ=255)

                                                                       gtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtg                  >  2:7832889/1‑93 (MQ=255)

                                                                        tggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtgg                 <  2:458768/93‑1 (MQ=255)

                                                                        tggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtgg                 <  1:3618186/93‑1 (MQ=255)

                                                                         gggCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtggt                <  1:5775703/93‑1 (MQ=255)

                                                                           gCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGc              >  1:1043364/1‑93 (MQ=255)

                                                                             tGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg            <  2:3639891/93‑1 (MQ=255)

                                                                             tGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg            >  1:7385330/1‑93 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           >  1:2458483/1‑93 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           <  1:2568377/93‑1 (MQ=255)

                                                                               gATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt          >  2:5472455/1‑93 (MQ=255)

                                                                               gATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggt          >  1:4741194/1‑93 (MQ=255)

                                                                                aTCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtg         >  2:5797727/1‑93 (MQ=255)

                                                                                aTCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtg         >  1:2705120/1‑93 (MQ=255)

                                                                                 tCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtgg        >  1:6950602/1‑93 (MQ=255)

                                                                                  ccTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtggt       <  2:6135534/93‑1 (MQ=255)

                                                                                      gcgtgcgtCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctc   <  1:228277/93‑1 (MQ=255)

                                                                                      gcgtgcgtCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctc   <  2:2360515/93‑1 (MQ=255)

                                                                                       cGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctct  >  1:4489563/1‑93 (MQ=255)

GCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCT  >  NC_000913/285685‑285864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`