Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,644,497 A→G R283R (CGT→CGC)  pbpC ← putative peptidoglycan enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,644,4970AG99.6% 858.0 / ‑5.2 272R283R (CGT→CGC) pbpCputative peptidoglycan enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (117/154);  total (117/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

Only 100 of 280 total aligned reads displayed.

AACACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTG  >  NC_000913/2644407‑2644586
                                                                                          |                                                                                         
 acacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        <  2:1453668/93‑1 (MQ=255)
 acacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        <  2:34738/93‑1 (MQ=255)
 acacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        <  2:4364368/93‑1 (MQ=255)
 acacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        >  2:4884981/1‑93 (MQ=255)
 acacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgt                                                                                        >  2:5573370/1‑93 (MQ=255)
  cacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCTAGGcgtc                                                                                       <  2:7762626/93‑1 (MQ=255)
  cacGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgtc                                                                                       >  1:6576469/1‑93 (MQ=255)
   acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGcgtcg                                                                                      >  2:5961062/1‑93 (MQ=255)
     gCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGtt                                                                                    >  1:7113581/1‑93 (MQ=255)
       atatCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGa                                                                                  <  2:6269060/93‑1 (MQ=255)
          tCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGCTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAgc                                                                               >  2:2947067/1‑92 (MQ=255)
           cGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGAc                                                                              <  1:2567745/93‑1 (MQ=255)
           cGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGAc                                                                              <  1:1269480/93‑1 (MQ=255)
           cGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGAc                                                                              <  1:1177758/93‑1 (MQ=255)
            ggTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGAcc                                                                             <  2:7640268/93‑1 (MQ=255)
            ggTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGAcc                                                                             >  1:3191485/1‑93 (MQ=255)
              tATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCgg                                                                           >  2:69894/1‑93 (MQ=255)
              tATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCgg                                                                           <  1:1020414/93‑1 (MQ=255)
                tGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCa                                                                         <  2:6136246/93‑1 (MQ=255)
                tGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCa                                                                         <  1:7288423/93‑1 (MQ=255)
                tGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCa                                                                         <  1:824946/93‑1 (MQ=255)
                tGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCa                                                                         >  1:4847897/1‑93 (MQ=255)
                tGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCa                                                                         >  1:2709300/1‑93 (MQ=255)
                  aTCAACCGCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATc                                                                       <  2:1633622/93‑1 (MQ=255)
                     aaCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCaa                                                                    <  1:7847627/93‑1 (MQ=255)
                      aCCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAAc                                                                   <  1:7697040/93‑1 (MQ=255)
                        cACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACga                                                                 >  1:1071035/1‑92 (MQ=255)
                         aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                >  1:5174935/1‑93 (MQ=255)
                         aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                >  1:3576271/1‑93 (MQ=255)
                         aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                <  1:4978711/93‑1 (MQ=255)
                         aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                <  1:3753853/93‑1 (MQ=255)
                         aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgta                                                                <  1:343022/93‑1 (MQ=255)
                          cGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtag                                                               <  1:2938823/93‑1 (MQ=255)
                           gATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagt                                                              <  1:6905406/93‑1 (MQ=255)
                            atcatcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagta                                                             >  2:46239/1‑93 (MQ=255)
                            atcatcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACgtagta                                                             <  1:5316224/93‑1 (MQ=255)
                             tcatcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGtagtag                                                            >  1:7905597/1‑93 (MQ=255)
                              catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTagtagt                                                           <  2:1191638/93‑1 (MQ=255)
                              catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTagtagt                                                           <  1:5934536/93‑1 (MQ=255)
                              catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTagtagt                                                           >  1:6338341/1‑93 (MQ=255)
                               atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTg                                                          <  1:2032677/93‑1 (MQ=255)
                                tcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGa                                                         >  1:2224938/1‑93 (MQ=255)
                                tcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGa                                                         <  1:545873/93‑1 (MQ=255)
                                tcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGa                                                         >  1:1533222/1‑93 (MQ=255)
                                tcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGa                                                         <  2:7163523/93‑1 (MQ=255)
                                   ccAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGAttt                                                      >  2:2626085/1‑93 (MQ=255)
                                     aGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTg                                                    >  2:5827496/1‑93 (MQ=255)
                                       tGAGCTTCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTc                                                  >  2:6370399/1‑93 (MQ=255)
                                         aGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgc                                                <  1:7548445/93‑1 (MQ=255)
                                             gcgcGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgctttt                                            <  2:1815731/93‑1 (MQ=255)
                                              cgcgGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttg                                           <  1:7499935/93‑1 (MQ=255)
                                                  gTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgcttt                                       >  2:7359201/1‑93 (MQ=255)
                                                  gTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgcttt                                       >  1:5165418/1‑93 (MQ=255)
                                                   tGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctttt                                      <  2:2358403/93‑1 (MQ=255)
                                                   tGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCgcttttgctttt                                      >  1:4501508/1‑93 (MQ=255)
                                                     gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTAc                                    >  1:7817475/1‑93 (MQ=255)
                                                      cAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTAcc                                   <  1:3986866/93‑1 (MQ=255)
                                                       aaCCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCg                                  <  1:4037470/93‑1 (MQ=255)
                                                       aaCCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCg                                  >  2:4509972/1‑93 (MQ=255)
                                                        atcGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGa                                 <  1:5237132/91‑1 (MQ=255)
                                                         ccGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAg                                >  2:3708854/1‑93 (MQ=255)
                                                           gCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGca                              >  2:5145168/1‑93 (MQ=255)
                                                           gCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGca                              >  1:5595982/1‑93 (MQ=255)
                                                           gCCCTTTCCAGTTTTGCGCCAATTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGca                              >  2:5015509/1‑93 (MQ=255)
                                                            cccTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcat                             >  2:4313264/1‑93 (MQ=255)
                                                            cccTTTCCAGTTTTGCGCCAGTTCGTCCAGGCGTCGTTGAAGACAGGCATCCAACGTAGGAGTGATTTTGTCGCTTTTGCTTTTACCGAGcag                             >  1:3693169/1‑92 (MQ=255)
                                                              cTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatct                           >  1:4699796/1‑92 (MQ=255)
                                                              cTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatca                           <  1:3811180/93‑1 (MQ=255)
                                                              cTTTCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatca                           >  2:2450338/1‑93 (MQ=255)
                                                               tttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatcat                          >  2:3438128/1‑93 (MQ=255)
                                                               tttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatcat                          <  2:3962553/93‑1 (MQ=255)
                                                               tttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatcat                          >  2:6480431/1‑93 (MQ=255)
                                                               tttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGcatcat                          >  1:2627862/1‑93 (MQ=255)
                                                                ttCGAGTTTTGCGCTAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATg                         <  2:336153/93‑1 (MQ=255)
                                                                ttCCAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATg                         <  1:306024/93‑1 (MQ=255)
                                                                   cAGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATgcga                      >  1:5847632/1‑92 (MQ=255)
                                                                    aGTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGcgcg                     >  1:4209595/1‑93 (MQ=255)
                                                                     gTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGa                    >  1:7231253/1‑93 (MQ=255)
                                                                     gTTTTGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATAATGCGCGa                    >  2:5708491/1‑93 (MQ=255)
                                                                       tttGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGata                  >  1:5791430/1‑91 (MQ=255)
                                                                       tttGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGaaa                  <  1:7360447/93‑1 (MQ=255)
                                                                         tGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAAc                <  2:2781232/93‑1 (MQ=255)
                                                                         tGCGCCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAAc                <  1:1650968/93‑1 (MQ=255)
                                                                          gcgcCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACa               <  2:6690129/93‑1 (MQ=255)
                                                                           cgcCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAg              <  1:4187344/93‑1 (MQ=255)
                                                                           cgcCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAg              <  2:7522646/93‑1 (MQ=255)
                                                                            gcCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGc             <  1:1752924/93‑1 (MQ=255)
                                                                            gcCAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGGAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGc             >  1:4621663/1‑93 (MQ=255)
                                                                             ccAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCg            <  2:4788376/93‑1 (MQ=255)
                                                                              cAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCgg           >  1:6794128/1‑93 (MQ=255)
                                                                              cAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCgg           >  1:1870130/1‑93 (MQ=255)
                                                                              cAGTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGCAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCgg           >  1:6255289/1‑93 (MQ=255)
                                                                                gTTCTTCCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTg         >  1:4409979/1‑93 (MQ=255)
                                                                                   cttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCa      <  1:3163588/93‑1 (MQ=255)
                                                                                   cttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCa      <  2:2180729/93‑1 (MQ=255)
                                                                                   cttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCa      <  2:2289493/93‑1 (MQ=255)
                                                                                    ttcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAg     <  2:2871974/93‑1 (MQ=255)
                                                                                     tcCAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGGCAGt    >  2:1440161/1‑93 (MQ=255)
                                                                                      ccAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGtt   <  1:3505820/93‑1 (MQ=255)
                                                                                      ccAGGCGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGtt   <  1:4375135/93‑1 (MQ=255)
                                                                                          |                                                                                         
AACACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTG  >  NC_000913/2644407‑2644586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑