BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,865	T→A	L135Q (CTG→CAG)	suhB →	SuhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,865	0	T	A	99.6%	894.4 / ‑3.8	245	L135Q (CTG→CAG)	suhB	SuhB
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (115/129); total (115/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

Only 100 of 256 total aligned reads displayed.

TTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACG  >  NC_000913/2661775‑2661954

 tCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGaa                                                                                        <  2:7855230/93‑1 (MQ=255)

  cGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAAc                                                                                       >  2:3157186/1‑93 (MQ=255)

   gTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACg                                                                                      <  1:350023/93‑1 (MQ=255)

    tATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACgg                                                                                     <  2:3686977/93‑1 (MQ=255)

     aTCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGc                                                                                    >  1:5340844/1‑93 (MQ=255)

     aTCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGc                                                                                    >  2:4001287/1‑93 (MQ=255)

      tCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCt                                                                                   >  2:1032583/1‑93 (MQ=255)

      tCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCt                                                                                   >  1:4229771/1‑93 (MQ=255)

      tCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCt                                                                                   >  1:724358/1‑93 (MQ=255)

       cAAAGGCCGCACCGAATGTGCTGTGGTATACGATCCTATTCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTa                                                                                  <  2:5490876/93‑1 (MQ=255)

          aGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCg                                                                               >  1:7690846/1‑93 (MQ=255)

           ggCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCgc                                                                              >  1:6007353/1‑92 (MQ=255)

           ggCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGa                                                                              <  2:1889014/93‑1 (MQ=255)

            gCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGAc                                                                             <  1:6133617/93‑1 (MQ=255)

             ccGCCCCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACt                                                                            <  1:2867420/93‑1 (MQ=255)

             ccGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACt                                                                            >  2:6479261/1‑93 (MQ=255)

             ccGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACt                                                                            <  1:2302623/93‑1 (MQ=255)

               gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                          <  1:5634221/93‑1 (MQ=255)

               gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                          <  1:3870172/93‑1 (MQ=255)

                cACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgcg                                                                         >  1:1260789/1‑93 (MQ=255)

                 aCCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgcgc                                                                        <  1:7764212/93‑1 (MQ=255)

                 aCCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgcgc                                                                        <  1:6394031/93‑1 (MQ=255)

                   cGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCCCTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCgg                                                                      <  1:5114053/93‑1 (MQ=255)

                    gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                     >  2:4309762/1‑93 (MQ=255)

                    gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                     <  1:1012442/93‑1 (MQ=255)

                    gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                     <  1:6851716/93‑1 (MQ=255)

                     aaGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgca                                                                    >  1:4350216/1‑93 (MQ=255)

                       gTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACTGCAGAACGGCTACGGACTGCGCGgcagc                                                                  >  2:6822241/1‑93 (MQ=255)

                       gTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgcagc                                                                  <  1:6513910/93‑1 (MQ=255)

                         tGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCAc                                                                >  1:5013659/1‑93 (MQ=255)

                         tGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCAc                                                                >  2:5401001/1‑93 (MQ=255)

                          gCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCAcc                                                               <  2:3825049/93‑1 (MQ=255)

                          gCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCAcc                                                               <  2:260022/93‑1 (MQ=255)

                            tgtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGc                                                             >  2:7430352/1‑93 (MQ=255)

                              tgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTc                                                           >  1:4786046/1‑93 (MQ=255)

                              tgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTc                                                           >  1:1957276/1‑93 (MQ=255)

                               ggTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTcg                                                          >  2:5216032/1‑93 (MQ=255)

                               ggTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTcg                                                          >  2:2529033/1‑93 (MQ=255)

                               ggTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTcg                                                          >  2:5274931/1‑93 (MQ=255)

                                gTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTcgc                                                         >  2:6162498/1‑93 (MQ=255)

                                  ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                       >  2:3311159/1‑93 (MQ=255)

                                  ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                       >  1:3889605/1‑93 (MQ=255)

                                  ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                       <  2:2025215/93‑1 (MQ=255)

                                    aCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtc                                                     >  1:4868895/1‑93 (MQ=255)

                                    aCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtc                                                     <  2:5910616/93‑1 (MQ=255)

                                    aCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtc                                                     <  1:536005/93‑1 (MQ=255)

                                      gATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtctc                                                   <  1:4573753/93‑1 (MQ=255)

                                      gATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtctc                                                   <  2:6807565/93‑1 (MQ=255)

                                      gATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtctc                                                   >  2:7121636/1‑93 (MQ=255)

                                       aTCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCg                                                  >  1:2087320/1‑93 (MQ=255)

                                       aTCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCg                                                  >  2:69386/1‑93 (MQ=255)

                                         ccTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAc                                                >  1:2480278/1‑93 (MQ=255)

                                         ccTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAc                                                <  1:702691/93‑1 (MQ=255)

                                          cTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACg                                               <  2:6716915/93‑1 (MQ=255)

                                          cTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACg                                               <  2:3218219/93‑1 (MQ=255)

                                           tATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACgg                                              <  2:215593/93‑1 (MQ=255)

                                            aTGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGt                                             >  2:1185639/1‑93 (MQ=255)

                                              gCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTAc                                           >  1:7425302/1‑93 (MQ=255)

                                                gTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTa                                         >  1:2747596/1‑93 (MQ=255)

                                                gTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTa                                         <  2:2679209/93‑1 (MQ=255)

                                                 tAACGACCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAt                                        <  2:4551587/93‑1 (MQ=255)

                                                 tAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAt                                        <  2:6364281/93‑1 (MQ=255)

                                                 tAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAt                                        <  1:6342733/93‑1 (MQ=255)

                                                    cGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                     <  2:2288944/93‑1 (MQ=255)

                                                     gAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTg                                    >  2:6185106/1‑93 (MQ=255)

                                                      aaCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTgg                                   >  2:7413397/1‑93 (MQ=255)

                                                        cTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCg                                 >  1:1716216/1‑93 (MQ=255)

                                                        cTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAATCTGGCg                                 >  2:465999/1‑93 (MQ=255)

                                                          gTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAc                               <  2:4964589/93‑1 (MQ=255)

                                                           ttCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAcc                              <  1:6679344/93‑1 (MQ=255)

                                                            tCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCg                             <  1:7905924/93‑1 (MQ=255)

                                                            tCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCg                             <  2:6045420/93‑1 (MQ=255)

                                                            tCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCACGCGATCTCGACGGTACTATTCTGGCGACCg                             <  2:1642262/93‑1 (MQ=255)

                                                              cccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGc                           <  2:4946205/92‑1 (MQ=255)

                                                              aCCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGc                           <  1:7654642/93‑1 (MQ=255)

                                                               ccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCt                          >  2:296200/1‑93 (MQ=255)

                                                               ccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCt                          <  2:4098095/93‑1 (MQ=255)

                                                                 gCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTc                        >  1:3035034/1‑93 (MQ=255)

                                                                 gCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGAGACCAGCTTc                        >  1:6432833/1‑93 (MQ=255)

                                                                  ccACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTcc                       <  1:6783288/93‑1 (MQ=255)

                                                                  ccACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTcc                       <  2:2925166/93‑1 (MQ=255)

                                                                   cACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTccc                      <  1:2877889/93‑1 (MQ=255)

                                                                   cACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGGACTATTCTGGCGACCGGCTTccc                      >  2:3758842/1‑93 (MQ=255)

                                                                     cTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGt                    <  1:2986525/93‑1 (MQ=255)

                                                                     cTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGt                    <  2:5001040/93‑1 (MQ=255)

                                                                     cTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGt                    <  1:652897/93‑1 (MQ=255)

                                                                      tCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGtt                   >  2:7822727/1‑93 (MQ=255)

                                                                         cgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCaa                <  2:1482782/93‑1 (MQ=255)

                                                                           gTCGGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAg              <  1:1332172/93‑1 (MQ=255)

                                                                           gTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCaaac              >  2:395783/1‑92 (MQ=255)

                                                                           gTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAg              <  2:5818657/93‑1 (MQ=255)

                                                                              aGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaa           <  2:7529865/93‑1 (MQ=255)

                                                                               gggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaa          >  1:1078682/1‑93 (MQ=255)

                                                                                ggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaaa         <  2:422536/93‑1 (MQ=255)

                                                                                ggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaaa         <  1:3714744/93‑1 (MQ=255)

                                                                                 gcgcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAAc        <  1:1799406/93‑1 (MQ=255)

                                                                                   gcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAg      >  2:4807243/1‑93 (MQ=255)

                                                                                   gcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAg      <  1:6351850/93‑1 (MQ=255)

                                                                                     acaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTa    >  2:3482470/1‑93 (MQ=255)

                                                                                      cagcagAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAc   <  1:3792413/93‑1 (MQ=255)

TTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACG  >  NC_000913/2661775‑2661954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`