BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	288,283	A→G	L35P (CTG→CCG)	yagI ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	288,283	0	A	G	99.6%	824.8 / ‑4.0	263	coding (104/759 nt)	yagI	putative regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (117/145); minor base . (1/0); total (118/145)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

Only 100 of 267 total aligned reads displayed.

GAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGAC  >  NC_000913/288195‑288372

gAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGccc                                                                                       >  2:5392598/1‑93 (MQ=255)

gAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGccc                                                                                       <  2:6547744/93‑1 (MQ=255)

   gCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCAt                                                                                    <  2:5098995/93‑1 (MQ=255)

    cGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATc                                                                                   >  2:7527505/1‑93 (MQ=255)

    cGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATc                                                                                   >  2:443417/1‑93 (MQ=255)

     gATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCa                                                                                  >  1:7690654/1‑93 (MQ=255)

     gATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCa                                                                                  <  2:3548577/93‑1 (MQ=255)

      aTACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAg                                                                                 >  2:365499/1‑93 (MQ=255)

        aCTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGtt                                                                               >  2:556603/1‑93 (MQ=255)

        aCTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGtt                                                                               <  2:3695043/93‑1 (MQ=255)

         cTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGACCCCATCAGttt                                                                              <  1:1236696/93‑1 (MQ=255)

           tGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGc                                                                            <  1:5223386/93‑1 (MQ=255)

             ccGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTg                                                                          >  1:3729244/1‑93 (MQ=255)

              cGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGa                                                                         <  2:7091288/93‑1 (MQ=255)

               gTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGAt                                                                        >  1:2492733/1‑93 (MQ=255)

                ttCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATg                                                                       <  2:248458/93‑1 (MQ=255)

                  ctcCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTc                                                                     <  1:3867895/93‑1 (MQ=255)

                   tcCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCg                                                                    >  1:253911/1‑93 (MQ=255)

                   tcCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCg                                                                    >  1:1001199/1‑93 (MQ=255)

                    ccGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCgg                                                                   <  1:7246715/93‑1 (MQ=255)

                     cGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGt                                                                  >  1:4547532/1‑93 (MQ=255)

                     cGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGt                                                                  <  2:6754989/93‑1 (MQ=255)

                      gggTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTg                                                                 <  2:464362/93‑1 (MQ=255)

                       ggTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGa                                                                <  2:3872060/93‑1 (MQ=255)

                       ggTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGa                                                                <  1:5620628/93‑1 (MQ=255)

                       ggTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGa                                                                >  1:5867398/1‑93 (MQ=255)

                         ttCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATc                                                              <  2:5780021/93‑1 (MQ=255)

                         ttCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATc                                                              <  2:3400690/93‑1 (MQ=255)

                            tGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCGTa                                                           <  2:1016984/93‑1 (MQ=255)

                             gATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTaa                                                          >  1:2964701/1‑93 (MQ=255)

                             gATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTaa                                                          <  1:2175427/93‑1 (MQ=255)

                             gATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTaa                                                          >  2:1134026/1‑93 (MQ=255)

                             gATCGATATAGCCGTGAAGCTGCAGGGTTCTTAGCAGCGAGTGGAGGGTCCTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTaa                                                          <  2:5647574/93‑1 (MQ=255)

                              aTCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGGGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAg                                                         >  2:7335006/1‑93 (MQ=255)

                               tCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGc                                                        >  2:2739934/1‑93 (MQ=255)

                                cGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCt                                                       >  2:7529907/1‑93 (MQ=255)

                                cGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCt                                                       <  2:1569393/93‑1 (MQ=255)

                                  atatAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCg                                                     <  1:5599863/93‑1 (MQ=255)

                                  atatAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCg                                                     >  1:4320065/1‑93 (MQ=255)

                                   tataGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCgg                                                    >  1:1973783/1‑93 (MQ=255)

                                   tataGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCgg                                                    >  1:6689463/1‑93 (MQ=255)

                                    ataGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGt                                                   <  1:763663/93‑1 (MQ=255)

                                    ataGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGt                                                   <  2:7908737/93‑1 (MQ=255)

                                    ataGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGt                                                   <  1:1946399/93‑1 (MQ=255)

                                     taGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTg                                                  >  1:3635187/1‑93 (MQ=255)

                                          gTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTg                                             >  1:1520867/1‑93 (MQ=255)

                                             aaGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTc                                          >  1:1111945/1‑93 (MQ=255)

                                              aGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCg                                         >  1:5840274/1‑93 (MQ=255)

                                               gCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGt                                        <  2:1263358/93‑1 (MQ=255)

                                               gCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGt                                        >  1:2909944/1‑93 (MQ=255)

                                                   cAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGaa                                    >  1:6906653/1‑93 (MQ=255)

                                                    aGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAAc                                   <  1:1646967/93‑1 (MQ=255)

                                                    aGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAAc                                   >  1:2819239/1‑93 (MQ=255)

                                                     gggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCTATCAGTTTGCTGATGTCTGTGTTTTTTAGCTCGGTGTCCTGCTCGTTGAAct                                  >  2:7004151/1‑92 (MQ=255)

                                                     gggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACa                                  <  1:6267394/93‑1 (MQ=255)

                                                     gggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACa                                  <  1:7077796/93‑1 (MQ=255)

                                                     gggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACa                                  >  1:4001643/1‑93 (MQ=255)

                                                      ggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAg                                 >  1:648680/1‑93 (MQ=255)

                                                      ggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAg                                 >  2:5527886/1‑93 (MQ=255)

                                                      ggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAg                                 <  1:565208/93‑1 (MQ=255)

                                                      ggTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAg                                 <  2:2620217/93‑1 (MQ=255)

                                                       gTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAgg                                >  1:4845383/1‑93 (MQ=255)

                                                       gTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATATTAAGCTCGGTGGCCTGCTCGTTGAACAgg                                <  2:2282402/93‑1 (MQ=255)

                                                        tttttAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGt                               >  1:7920033/1‑93 (MQ=255)

                                                           ttAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGa                            <  2:3130882/93‑1 (MQ=255)

                                                           ttAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGa                            <  1:5510132/93‑1 (MQ=255)

                                                           ttAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGa                            <  2:393419/93‑1 (MQ=255)

                                                           ttAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGa                            <  1:4183166/93‑1 (MQ=255)

                                                           ttAGCAGCGAGAGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGa                            >  2:4568028/1‑93 (MQ=255)

                                                            tAGCAGCGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAg                           <  2:6909507/93‑1 (MQ=255)

                                                             agcagcGAGTGGAGGGTTCTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAgg                          <  2:6405182/93‑1 (MQ=255)

                                                              gcagcGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAggt                         >  1:5396786/1‑92 (MQ=255)

                                                              gcagcGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGa                         <  2:6269757/93‑1 (MQ=255)

                                                               cagcGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGAt                        <  2:1177491/93‑1 (MQ=255)

                                                                agcGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATc                       <  2:7681565/93‑1 (MQ=255)

                                                                agcGAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATc                       <  1:3810295/93‑1 (MQ=255)

                                                                   gAGTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGc                    >  1:4912453/1‑93 (MQ=255)

                                                                   gAGTGGAGGAGACTCTTCCTCGGCCCCATCAGTTTGGTGATGTCGGTGATCTTAACCTGGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGc                    <  2:4149350/93‑1 (MQ=255)

                                                                     gTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCaa                  >  2:222359/1‑93 (MQ=255)

                                                                     gTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCaa                  <  2:714069/93‑1 (MQ=255)

                                                                     gTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCaa                  >  2:1739523/1‑93 (MQ=255)

                                                                     gTGGAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCaa                  <  2:6074375/93‑1 (MQ=255)

                                                                       ggAGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACg                <  2:3506177/93‑1 (MQ=255)

                                                                         aGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCa              <  1:7818432/93‑1 (MQ=255)

                                                                         aGGGTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCa              <  1:7444469/93‑1 (MQ=255)

                                                                          gggTACTCTTGCTCGGCTCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCAc             >  2:5288376/1‑93 (MQ=255)

                                                                          gggTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCAc             >  2:6306518/1‑93 (MQ=255)

                                                                          gggTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCAc             <  1:3565085/93‑1 (MQ=255)

                                                                            gTACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGt           <  1:861985/93‑1 (MQ=255)

                                                                             tACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGtt          >  1:1450196/1‑93 (MQ=255)

                                                                             tACTCTTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGtt          >  2:6818865/1‑93 (MQ=255)

                                                                              tctctTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTc         <  1:5133769/92‑1 (MQ=255)

                                                                               ctctTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCa        <  2:5962413/93‑1 (MQ=255)

                                                                                 ctTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGTACAGGTCGAGGATCTGCAACGCACGTTCAAc      <  1:92799/93‑1 (MQ=255)

                                                                                 ctTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAAc      <  1:7334892/93‑1 (MQ=255)

                                                                                 ctTGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAAc      <  2:6654407/93‑1 (MQ=255)

                                                                                  ttGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACa     >  2:6202599/1‑93 (MQ=255)

                                                                                  ttGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACa     <  1:3081932/93‑1 (MQ=255)

                                                                                  ttGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACa     >  2:2006462/1‑93 (MQ=255)

                                                                                  ttGCTCGGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACa     <  1:5935414/93‑1 (MQ=255)

GAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGAC  >  NC_000913/288195‑288372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`