BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,699,813	T→C	E226G (GAA→GGA)	recO ←	RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,699,813	0	T	C	99.6%	714.3 / ‑4.9	225	E226G (GAA→GGA)	recO	RecO
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (120/104); total (120/105)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 236 total aligned reads displayed.

GTTGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGG  >  NC_000913/2699723‑2699901

 ttGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTcccc                                                                                       <  2:5295454/93‑1 (MQ=255)

  tGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCt                                                                                      <  2:1023526/93‑1 (MQ=255)

    aCGTCTAACAGTAATTTAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGc                                                                                    <  1:2702747/93‑1 (MQ=255)

      gCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGctc                                                                                  <  2:3077231/93‑1 (MQ=255)

       ccTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAATTGCCGGAACAGTCCCCTGctct                                                                                 >  1:6622405/1‑93 (MQ=255)

        cTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCtt                                                                                <  1:110550/93‑1 (MQ=255)

        cTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCtt                                                                                >  1:4896051/1‑93 (MQ=255)

         tAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCttt                                                                               >  2:4189454/1‑93 (MQ=255)

          aaCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTa                                                                              <  1:4251196/93‑1 (MQ=255)

           aCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTat                                                                             >  1:4048625/1‑92 (MQ=255)

           aCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTaa                                                                             >  2:6028452/1‑93 (MQ=255)

           aCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTaa                                                                             <  1:3922202/93‑1 (MQ=255)

             aGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAg                                                                           >  1:7465591/1‑93 (MQ=255)

                aaTTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGtt                                                                        >  1:1286711/1‑93 (MQ=255)

                 aTTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGttt                                                                       <  2:6242620/93‑1 (MQ=255)

                  ttCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTa                                                                      >  1:2277765/1‑93 (MQ=255)

                  atCAGCCATTACAATCCTCATCATTCATAATGTGTTTTCAACGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTa                                                                      <  1:2337163/92‑1 (MQ=255)

                   tCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTAc                                                                     <  2:5220663/93‑1 (MQ=255)

                    cAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTAcc                                                                    <  2:6366298/93‑1 (MQ=255)

                      gCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGc                                                                  <  2:4035062/93‑1 (MQ=255)

                      gCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGc                                                                  >  1:5916801/1‑93 (MQ=255)

                      gCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGc                                                                  >  1:7381057/1‑93 (MQ=255)

                       ccATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGcc                                                                 <  2:7366668/93‑1 (MQ=255)

                       ccATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGcc                                                                 >  1:4206671/1‑93 (MQ=255)

                        cATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCa                                                                >  2:7344052/1‑93 (MQ=255)

                        cATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCa                                                                >  2:1787683/1‑93 (MQ=255)

                        cATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCa                                                                >  1:2869705/1‑93 (MQ=255)

                        cATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCa                                                                >  1:1924739/1‑93 (MQ=255)

                        cATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCa                                                                >  2:1475546/1‑93 (MQ=255)

                          tGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAg                                                              >  1:536250/1‑93 (MQ=255)

                           gACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGa                                                             <  1:4741810/93‑1 (MQ=255)

                            aCAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGAt                                                            <  1:2454936/93‑1 (MQ=255)

                              aaTCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATAc                                                          <  2:4727843/93‑1 (MQ=255)

                               aTCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACg                                                         >  1:3501341/1‑93 (MQ=255)

                                tCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACgg                                                        >  2:1508066/1‑93 (MQ=255)

                                  cTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCt                                                      >  1:4157383/1‑93 (MQ=255)

                                     atcatTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTaa                                                   <  1:5719439/93‑1 (MQ=255)

                                     atcatTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTaa                                                   <  1:580713/93‑1 (MQ=255)

                                     atcatTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTaa                                                   >  1:457484/1‑93 (MQ=255)

                                      tcattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAg                                                  >  1:7212365/1‑93 (MQ=255)

                                      tcattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAg                                                  >  1:7094896/1‑93 (MQ=255)

                                      tcattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAg                                                  >  2:1878734/1‑93 (MQ=255)

                                       cattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgc                                                 <  1:6350813/93‑1 (MQ=255)

                                       cattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgc                                                 <  2:7465658/93‑1 (MQ=255)

                                       cattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgc                                                 >  2:1242998/1‑93 (MQ=255)

                                         ttcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcgc                                               >  1:5683779/1‑93 (MQ=255)

                                           catAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACGGCCAAGATACGGCTTAAGCGCCa                                             >  2:4299130/1‑93 (MQ=255)

                                           catAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCa                                             <  2:3254110/93‑1 (MQ=255)

                                           catAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCa                                             <  1:2114920/93‑1 (MQ=255)

                                            atAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCAt                                            <  2:6726547/93‑1 (MQ=255)

                                            atAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCAt                                            <  1:2489766/93‑1 (MQ=255)

                                             tAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATg                                           >  1:1860575/1‑93 (MQ=255)

                                             tAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATg                                           >  2:251270/1‑93 (MQ=255)

                                              aaTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGc                                          >  1:7290853/1‑93 (MQ=255)

                                               aTGTGTTTTAACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCg                                         >  2:6385949/1‑93 (MQ=255)

                                                 gtgtTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCggg                                       <  1:7710711/93‑1 (MQ=255)

                                                  tgtTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGt                                      <  2:6230697/93‑1 (MQ=255)

                                                    ttttCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTaa                                    <  2:4528226/93‑1 (MQ=255)

                                                      ttCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAg                                  >  1:3625389/1‑93 (MQ=255)

                                                      ttCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAg                                  <  1:1873219/93‑1 (MQ=255)

                                                       tCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGc                                 >  2:5604502/1‑93 (MQ=255)

                                                         aCCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGt                               >  2:5904873/1‑93 (MQ=255)

                                                          ccGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGtt                              <  2:7065873/93‑1 (MQ=255)

                                                           cGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGttt                             <  2:2230370/93‑1 (MQ=255)

                                                            gTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTc                            <  1:4003230/93‑1 (MQ=255)

                                                             ttCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcg                           >  2:1618261/1‑93 (MQ=255)

                                                             ttCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcg                           >  2:2781908/1‑93 (MQ=255)

                                                             ttCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcg                           >  1:3169850/1‑93 (MQ=255)

                                                             ttCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcg                           >  1:5533214/1‑93 (MQ=255)

                                                             ttCGCTTAGGCATAAACTGCCGGAACACTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcg                           >  1:5046459/1‑93 (MQ=255)

                                                              tCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcgc                          <  1:7641793/93‑1 (MQ=255)

                                                               cGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcgcg                         >  1:4567232/1‑93 (MQ=255)

                                                                 cTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCgcggc                       >  1:3217/1‑93 (MQ=255)

                                                                  ttAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCgcggcg                      <  1:51918/93‑1 (MQ=255)

                                                                  ttAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGGTTCgcggcg                      >  1:5349632/1‑93 (MQ=255)

                                                                   tAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGgcgc                     >  2:3739798/1‑93 (MQ=255)

                                                                   tAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGgcgc                     <  2:1991110/93‑1 (MQ=255)

                                                                       cATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAg                 <  1:6818838/93‑1 (MQ=255)

                                                                       cATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAg                 <  2:5017183/93‑1 (MQ=255)

                                                                        aTAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgt                <  2:1667227/93‑1 (MQ=255)

                                                                        aTAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgt                >  2:5746447/1‑93 (MQ=255)

                                                                        aTAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgt                >  1:6496785/1‑93 (MQ=255)

                                                                        aTAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgt                <  2:3803756/93‑1 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  2:6679238/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  2:7921700/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  2:496302/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  2:3614875/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  2:2983892/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  2:218867/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  1:7780422/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg               >  1:4377838/1‑93 (MQ=255)

                                                                          aaaCTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgt              >  1:7173002/1‑93 (MQ=255)

                                                                           aaCTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgtg             >  1:7571882/1‑93 (MQ=255)

                                                                             cTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTc           <  2:6906716/93‑1 (MQ=255)

                                                                             cTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTc           <  1:4455080/93‑1 (MQ=255)

                                                                             cTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTc           <  1:2779091/93‑1 (MQ=255)

                                                                                ccGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGc        <  1:7494227/93‑1 (MQ=255)

                                                                                ccGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGc        <  2:6480512/93‑1 (MQ=255)

                                                                                ccGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGc        <  1:1048204/93‑1 (MQ=255)

                                                                                     aCAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAg   >  2:5918840/1‑93 (MQ=255)

GTTGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGG  >  NC_000913/2699723‑2699901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`