BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,460	T→C	I163T (ATC→ACC)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,460	0	T	C	99.7%	906.4 / ‑5.6	289	I163T (ATC→ACC)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (136/152); total (136/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 308 total aligned reads displayed.

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAccc                                                                                         >  1:548951/1‑93 (MQ=255)

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCt                                                                                        >  1:1473926/1‑93 (MQ=255)

  cAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTc                                                                                       <  1:2643941/93‑1 (MQ=255)

   aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCg                                                                                      >  1:7783479/1‑93 (MQ=255)

   aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCg                                                                                      >  1:6571444/1‑93 (MQ=255)

   aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCg                                                                                      >  1:2291410/1‑93 (MQ=255)

      gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACt                                                                                   >  2:7400512/1‑93 (MQ=255)

      gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACt                                                                                   <  1:7044443/93‑1 (MQ=255)

      gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTACCCAACACCAGCCACGACt                                                                                   >  2:5730714/1‑93 (MQ=255)

        cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTgg                                                                                 >  1:3872277/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTggg                                                                                <  2:2607391/93‑1 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                               >  1:6253860/1‑93 (MQ=255)

            aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgcgc                                                                             >  1:1597859/1‑93 (MQ=255)

             tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCa                                                                            <  1:3987713/93‑1 (MQ=255)

              gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCaa                                                                           <  2:7827462/93‑1 (MQ=255)

               ccAGTTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  2:5994884/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  2:3556688/93‑1 (MQ=255)

                cAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACa                                                                         <  1:5599898/93‑1 (MQ=255)

                cAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACa                                                                         >  2:2381294/1‑93 (MQ=255)

                 aGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAg                                                                        >  1:483569/1‑93 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCACCACCACCCTCGACTGGGCGCAACAGc                                                                       >  2:4486599/1‑93 (MQ=255)

                    ttttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGt                                                                     >  2:1517000/1‑93 (MQ=255)

                      ttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTaa                                                                   >  2:2808699/1‑93 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  1:4153928/93‑1 (MQ=255)

                         gCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAt                                                                <  1:2944050/93‑1 (MQ=255)

                           cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATgg                                                              >  2:6198147/1‑93 (MQ=255)

                           cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATgg                                                              <  2:5101460/93‑1 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             <  2:3917095/93‑1 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             >  1:2545361/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             >  2:6313844/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             <  1:6701390/93‑1 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           <  2:3045511/93‑1 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGGAAGATggggt                                                           >  2:2012238/1‑91 (MQ=255)

                                gcGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCCCCCTCGACTGGGCGCAACAGCGTAAGATGGGCttt                                                         <  1:337931/92‑1 (MQ=255)

                                   aTTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGGAAGATGGGCTTTTcc                                                      >  1:87230/1‑93 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCGACAGCGTAAGATGGGCTTTTCCt                                                     >  2:5411110/1‑93 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCt                                                     >  1:6366139/1‑93 (MQ=255)

                                    ttAAACGCGGCAAGCTGGAGTTTATTTCGCACTCGGCTGCCGTCTCCAAAACCCCCCTCGACTGGGCGCAACCGCGTAAGATGGGCTTTTCCt                                                     <  2:7908693/93‑1 (MQ=255)

                                     tAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                    <  2:611112/93‑1 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAc                                                   <  2:570966/93‑1 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAc                                                   >  2:7430859/1‑93 (MQ=255)

                                       aaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACt                                                  <  1:3264490/93‑1 (MQ=255)

                                       aaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACt                                                  <  1:6106668/93‑1 (MQ=255)

                                       aaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACt                                                  <  1:4243689/93‑1 (MQ=255)

                                        aCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACtt                                                 <  2:2261102/93‑1 (MQ=255)

                                          gcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTa                                               <  2:1205744/93‑1 (MQ=255)

                                          gcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGGCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTa                                               <  1:2093742/93‑1 (MQ=255)

                                           cgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAt                                              <  1:7633102/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  2:4669882/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  2:4967788/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  1:5750929/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  1:3469529/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             >  1:1309690/1‑93 (MQ=255)

                                             gCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTg                                            <  2:870575/93‑1 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCACTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           <  2:1409160/93‑1 (MQ=255)

                                               aaGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcg                                          >  2:4090553/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         >  2:6768451/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         <  1:1882054/93‑1 (MQ=255)

                                                 gCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCt                                        >  1:7412678/1‑93 (MQ=255)

                                                  cTGTCGTTTATTTCTCAATCGGCTGCCGTCTCCAACTCCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTc                                       <  1:1985448/93‑1 (MQ=255)

                                                  cTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCtg                                       >  2:6458779/1‑92 (MQ=255)

                                                  cTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTc                                       >  1:7230281/1‑93 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                      <  1:1118670/93‑1 (MQ=255)

                                                    ggCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATTGGCTTTTCCTACTTTATTGCGCTCgg                                     >  1:437171/1‑93 (MQ=255)

                                                    ggCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCgg                                     >  2:7503627/1‑93 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                    <  1:5208366/93‑1 (MQ=255)

                                                       gTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGa                                  >  2:6945628/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 <  1:7502446/93‑1 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:3513293/1‑93 (MQ=255)

                                                         ttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACa                                <  2:7739056/93‑1 (MQ=255)

                                                         ttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACa                                <  2:816762/93‑1 (MQ=255)

                                                         ttATTTCGAAATCGGCTGCCGTCTCCAACGCCTCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACa                                <  1:6905683/93‑1 (MQ=255)

                                                          tATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAg                               <  2:3292852/93‑1 (MQ=255)

                                                          tATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAg                               <  1:5700835/93‑1 (MQ=255)

                                                           aTTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGc                              >  1:4007308/1‑93 (MQ=255)

                                                                   aTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGatat                      >  2:3622641/1‑93 (MQ=255)

                                                                   aTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGatat                      <  2:5206279/93‑1 (MQ=255)

                                                                    tCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATc                     >  1:2576488/1‑93 (MQ=255)

                                                                      ggCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGa                   >  2:714504/1‑93 (MQ=255)

                                                                        cTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                 >  2:1690851/1‑93 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                <  2:3165648/93‑1 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                <  1:5867853/93‑1 (MQ=255)

                                                                          gCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGtt               <  2:1126986/93‑1 (MQ=255)

                                                                          gCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCCGCGACAGCCTGGATATCGACGtt               >  2:4337356/1‑93 (MQ=255)

                                                                          gCCGTCTCCAACAACACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGtt               >  2:3757045/1‑93 (MQ=255)

                                                                            cGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtga             >  1:5325721/1‑93 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg           >  1:1019014/1‑93 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg           >  2:6143188/1‑93 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  2:7215999/93‑1 (MQ=255)

                                                                                 ccAACACCCCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAt        <  1:3118171/93‑1 (MQ=255)

                                                                                 ccAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAt        >  2:7271324/1‑93 (MQ=255)

                                                                                  cAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAtt       <  1:5346072/93‑1 (MQ=255)

                                                                                  cAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAtt       <  2:5441271/93‑1 (MQ=255)

                                                                                   aaCACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTg      >  1:6176559/1‑93 (MQ=255)

                                                                                    acacCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGc     >  2:5589341/1‑93 (MQ=255)

                                                                                     cgccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    >  2:5494329/3‑93 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    <  2:4890424/93‑1 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    <  2:2153643/93‑1 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  >  1:957972/1‑93 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  <  1:469924/93‑1 (MQ=255)

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`