BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	297,171	A→G	R260R (CGT→CGC)	yagQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	297,171	0	A	G	98.4%	944.6 / ‑3.8	308	R260R (CGT→CGC)	yagQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (139/164); total (142/166)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

GCGCATCCCGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGACGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTT  >  NC_000913/297080‑297260

   cATCCCGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTa                                                                                       <  1:138159/93‑1 (MQ=255)

    aTCCCGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTaa                                                                                      >  1:5399678/1‑93 (MQ=255)

     tCCCGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAAc                                                                                     >  1:7719214/1‑93 (MQ=255)

     tCCCGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAAc                                                                                     <  2:6306256/93‑1 (MQ=255)

      cccGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACg                                                                                    >  2:3721425/1‑93 (MQ=255)

       ccGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACgt                                                                                   <  2:2867543/93‑1 (MQ=255)

       ccGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACgt                                                                                   <  1:6346679/93‑1 (MQ=255)

         gggCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACgtgt                                                                                 >  2:7204307/1‑93 (MQ=255)

         gggCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACgtgt                                                                                 >  1:5133749/1‑93 (MQ=255)

         gggCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACgtgt                                                                                 >  1:872504/1‑93 (MQ=255)

         gggCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACgtgg                                                                                 >  2:2222475/1‑92 (MQ=255)

           gCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTgg                                                                               <  1:4929671/93‑1 (MQ=255)

           gCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTgg                                                                               <  1:5257427/93‑1 (MQ=255)

               tGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTc                                                                           <  1:7676401/93‑1 (MQ=255)

                 gggAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGa                                                                         <  1:5843478/93‑1 (MQ=255)

                 gggAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGa                                                                         <  2:3174436/93‑1 (MQ=255)

                 gggAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGa                                                                         <  1:3178140/93‑1 (MQ=255)

                 gggAATATCCCGACGGGTGCCCGGATTTGCGTTGTATCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGa                                                                         <  2:837299/93‑1 (MQ=255)

                  ggAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGAt                                                                        >  1:2841501/1‑93 (MQ=255)

                  ggAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGAt                                                                        <  2:3630477/93‑1 (MQ=255)

                   gAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATa                                                                       >  1:7400559/1‑93 (MQ=255)

                    aaTATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAg                                                                      >  2:6259772/1‑93 (MQ=255)

                     atatCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGc                                                                     <  2:6597871/93‑1 (MQ=255)

                      tatCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGCCCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCt                                                                    <  1:3294972/93‑1 (MQ=255)

                        tCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGc                                                                  >  2:1911878/1‑93 (MQ=255)

                            gACGGTTGCCCGGATTTGCGTTCTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCaa                                                              <  2:3053912/93‑1 (MQ=255)

                            gACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCaa                                                              <  1:7535192/93‑1 (MQ=255)

                              cGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATg                                                            >  2:2329951/1‑93 (MQ=255)

                               gggTTCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATgc                                                           <  2:5500664/93‑1 (MQ=255)

                                 gTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATgcgc                                                         <  1:7252851/93‑1 (MQ=255)

                                    cccGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCga                                                      <  1:4428286/93‑1 (MQ=255)

                                    cccGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCga                                                      <  1:376697/93‑1 (MQ=255)

                                     ccGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCgag                                                     >  2:1797311/1‑93 (MQ=255)

                                     ccGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCgag                                                     >  2:4933590/1‑93 (MQ=255)

                                      cGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCgaga                                                    >  2:3684479/1‑93 (MQ=255)

                                      cGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGAGGAGCTTTTGCATGCGTAACGTGTGGGTTCGATAGCTGCCCAAAGCGCCgaga                                                    >  2:4338340/1‑93 (MQ=255)

                                       gaaTTTGCGTTGTTTCCTCCCTGGCCCAGCCCAGCTCGTGTAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCGATGCGCCGAGAt                                                   <  2:163828/91‑1 (MQ=255)

                                        gATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATa                                                  >  1:5125725/1‑93 (MQ=255)

                                        gATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATa                                                  >  1:6394762/1‑93 (MQ=255)

                                        gATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATa                                                  >  1:6774920/1‑93 (MQ=255)

                                        gATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTAGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATa                                                  >  2:7799199/1‑93 (MQ=255)

                                           ttGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATaaaa                                               <  1:2469289/93‑1 (MQ=255)

                                           ttGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATaaaa                                               <  2:912322/93‑1 (MQ=255)

                                           ttGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATaaaa                                               <  2:5892537/93‑1 (MQ=255)

                                           ttGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATaaaa                                               >  1:2952233/1‑93 (MQ=255)

                                           ttGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATaaaa                                               <  1:4726221/93‑1 (MQ=255)

                                            tGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAg                                              <  2:6496072/93‑1 (MQ=255)

                                             tcGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAgg                                             <  1:6272729/92‑1 (MQ=255)

                                             gCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCCATGCGCCGAGATAAAAgg                                             >  2:3007049/1‑93 (MQ=255)

                                             gCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAgg                                             <  2:1112474/93‑1 (MQ=255)

                                              cattgttACCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTATCGTGTGCGTTCAATAGATGCCCAATGCGACGAGATAAAAgag                                            <  2:6999538/91‑3 (MQ=255)

                                              cGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAggg                                            <  1:4039256/93‑1 (MQ=255)

                                              cGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAggg                                            <  2:7432101/93‑1 (MQ=255)

                                               gttgttTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGt                                           >  1:1085978/1‑93 (MQ=255)

                                                ttgttTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAgggct                                          >  2:262449/1‑91 (MQ=255)

                                                ttgttTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGtt                                          >  2:5431870/1‑93 (MQ=255)

                                                 tgttTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGttt                                         <  1:764675/93‑1 (MQ=255)

                                                  ttttCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGtttt                                        <  1:6110528/92‑1 (MQ=255)

                                                  gttTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGtttt                                        <  1:3404950/93‑1 (MQ=255)

                                                   tttCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTg                                       <  1:3699421/93‑1 (MQ=255)

                                                     tcctccCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCt                                     >  2:254740/1‑93 (MQ=255)

                                                      cctccCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCtt                                    >  1:1490845/1‑93 (MQ=255)

                                                       ctccCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTtc                                   <  1:3849512/93‑1 (MQ=255)

                                                       ctccCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTtc                                   >  1:4598602/1‑93 (MQ=255)

                                                        tccCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTtct                                  >  1:3588718/1‑93 (MQ=255)

                                                        tccCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTtct                                  <  1:2549670/93‑1 (MQ=255)

                                                         cccTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTtctc                                 >  1:183668/1‑93 (MQ=255)

                                                          ccTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcg                                >  2:5374888/1‑93 (MQ=255)

                                                          ccTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcg                                >  1:7927618/1‑93 (MQ=255)

                                                           cTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgc                               >  2:6767104/1‑93 (MQ=255)

                                                           cTGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgc                               <  2:5768969/93‑1 (MQ=255)

                                                            tGGACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgcg                              >  2:790760/1‑93 (MQ=255)

                                                             ggACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgcgc                             <  1:1665168/93‑1 (MQ=255)

                                                             ggACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgcgc                             >  1:498528/1‑93 (MQ=255)

                                                             ggACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgcgc                             <  1:126972/93‑1 (MQ=255)

                                                             ggACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTcgcgc                             >  1:6840052/1‑93 (MQ=255)

                                                              gACCATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGGTTTGCTTCTcgcgcg                            >  2:7089082/1‑93 (MQ=255)

                                                                ccATCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGc                          >  1:6054634/1‑93 (MQ=255)

                                                                   tCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTg                       >  1:2209090/1‑93 (MQ=255)

                                                                   tCCCAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTCGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTTGCGCGGCCTg                       >  1:306925/1‑93 (MQ=255)

                                                                      cAGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCaa                    >  2:1357042/1‑93 (MQ=255)

                                                                       aGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAac                   <  1:100525/93‑1 (MQ=255)

                                                                       aGCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAac                   <  1:4125618/93‑1 (MQ=255)

                                                                        gCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAaca                  <  2:4495193/93‑1 (MQ=255)

                                                                        gCTCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAaca                  <  1:4308816/93‑1 (MQ=255)

                                                                          tCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACt                >  2:419048/1‑93 (MQ=255)

                                                                          tCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACt                >  1:1405674/1‑93 (MQ=255)

                                                                          tCGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACt                >  1:2109297/1‑93 (MQ=255)

                                                                           cGTGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTg               >  1:7809662/1‑93 (MQ=255)

                                                                             tGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGc             >  2:3997192/1‑93 (MQ=255)

                                                                             tGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGc             <  1:5085550/93‑1 (MQ=255)

                                                                             tGGAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTGGCTTCTCGCGCGGCCTGCAACACTGGc             >  2:2782106/1‑93 (MQ=255)

                                                                              ggAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCa            >  2:332247/1‑93 (MQ=255)

                                                                               gAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAg           >  1:4176942/1‑93 (MQ=255)

                                                                               gAGCTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAg           >  2:2971318/1‑93 (MQ=255)

                                                                                  cTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTc        <  1:1949028/93‑1 (MQ=255)

                                                                                  cTTTTGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTc        <  1:2183360/93‑1 (MQ=255)

                                                                                   ttttGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTcc       >  1:5580920/1‑93 (MQ=255)

                                                                                   ttttGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTcc       <  2:5141103/93‑1 (MQ=255)

                                                                                    tttGCAGGCGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTccc      >  2:7278517/1‑93 (MQ=255)

GCGCATCCCGGGCTTTGGGGAATATCCCGACGGGTGCCCGGATTTGCGTTGTTTCCTCCCTGGACCATCCCAGCTCGTGGAGCTTTTGCAGACGTAACGTGTGGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTT  >  NC_000913/297080‑297260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`