BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,983	T→C	A44A (GCT→GCC)	yfjB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,983	0	T	C	99.7%	880.9 / ‑5.1	285	A44A (GCT→GCC)	yfjB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (151/132); total (151/134)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

Only 100 of 290 total aligned reads displayed.

ACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACG  >  NC_000913/2748893‑2749071

  ggCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACg                                                                                      >  1:3250002/1‑93 (MQ=255)

    cACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGaa                                                                                    <  2:7796964/93‑1 (MQ=255)

     aCCCCCCTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAAc                                                                                   <  1:3643551/93‑1 (MQ=255)

     aCCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAAc                                                                                   <  2:2914933/93‑1 (MQ=255)

     aCCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAAc                                                                                   >  1:5165412/1‑93 (MQ=255)

      ccccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACt                                                                                  >  1:4663787/1‑93 (MQ=255)

      ccccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGACCACGCACt                                                                                  <  2:1801904/93‑1 (MQ=255)

        ccACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGc                                                                                >  1:1319201/1‑93 (MQ=255)

         cactgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCa                                                                               <  2:734461/93‑1 (MQ=255)

         cactgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCa                                                                               >  2:6908580/1‑93 (MQ=255)

           ctgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAAc                                                                             <  1:2271494/93‑1 (MQ=255)

            tgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACt                                                                            <  1:7616034/93‑1 (MQ=255)

            tgcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACt                                                                            >  2:1564396/1‑93 (MQ=255)

             gcactgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTg                                                                           >  2:6730439/1‑93 (MQ=255)

               actgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACCAATCGCCCACGAAATGAAACTgaa                                                                         >  2:3024506/1‑93 (MQ=255)

               actgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaa                                                                         <  1:1116453/93‑1 (MQ=255)

                ctgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaag                                                                        >  2:4383459/1‑93 (MQ=255)

                ctgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaag                                                                        >  1:6281656/1‑93 (MQ=255)

                ctgACAACACATGAAATGCTCTACCGCTCGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAATAAATCGCCCACGAACTGCAACTgaag                                                                        >  1:3998159/1‑93 (MQ=255)

                 tgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaaga                                                                       >  2:1237238/1‑93 (MQ=255)

                 tgACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaaga                                                                       >  1:2682318/1‑93 (MQ=255)

                  gACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaagaa                                                                      <  2:7213711/93‑1 (MQ=255)

                  gACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaagaa                                                                      >  2:6033133/1‑93 (MQ=255)

                  gACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTgaagaa                                                                      <  1:5568633/93‑1 (MQ=255)

                    caacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtg                                                                    <  1:2834193/93‑1 (MQ=255)

                    caacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtg                                                                    >  1:1927986/1‑93 (MQ=255)

                    caacaCATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtg                                                                    >  1:2659738/1‑93 (MQ=255)

                     aacaAATTAAATGCTCTACCGCTGGCTTTGCGCAAAATGTTACGAGGTCATCCTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAAtgt                                                                   <  1:3848003/93‑1 (MQ=255)

                        acaTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaa                                                                >  2:470804/1‑93 (MQ=255)

                          aTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaaaa                                                              <  1:6629644/93‑1 (MQ=255)

                          aTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaaaa                                                              <  2:2691423/93‑1 (MQ=255)

                          aTGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGaaaa                                                              <  2:2742871/93‑1 (MQ=255)

                           tGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAAc                                                             <  2:2422877/93‑1 (MQ=255)

                           tGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAAc                                                             <  2:1322333/93‑1 (MQ=255)

                            gAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACt                                                            <  2:1701752/93‑1 (MQ=255)

                            gAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACt                                                            <  2:6362147/93‑1 (MQ=255)

                              aaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTgg                                                          >  2:4724131/1‑93 (MQ=255)

                              aaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTgg                                                          >  2:1076629/1‑93 (MQ=255)

                              aaTGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTgg                                                          >  1:1715668/1‑93 (MQ=255)

                                tGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGTACTGCAACTGAAGAATGTGAAAACTGGct                                                        <  2:2539493/93‑2 (MQ=255)

                                tGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCa                                                        <  1:7515929/93‑1 (MQ=255)

                                tGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCa                                                        >  1:2493868/1‑93 (MQ=255)

                                 gCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCAc                                                       <  1:6133398/93‑1 (MQ=255)

                                 gCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCAc                                                       >  1:5698706/1‑93 (MQ=255)

                                   tctACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGc                                                     >  1:527075/1‑93 (MQ=255)

                                        cGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTcgcg                                                >  1:7323582/1‑93 (MQ=255)

                                         gctggctgTGAACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCgg                                               >  2:3424577/1‑93 (MQ=255)

                                          ctggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAACCTGGCACGCTCGCGga                                              <  2:7520024/93‑1 (MQ=255)

                                          ctggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGga                                              >  2:4257111/1‑93 (MQ=255)

                                           tggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGgag                                             >  1:7930420/1‑93 (MQ=255)

                                           tggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGgag                                             >  1:5919585/1‑93 (MQ=255)

                                            ggctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGgaga                                            <  2:5757648/93‑1 (MQ=255)

                                              ctgTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCTCGGAGAtg                                          >  1:1534133/1‑92 (MQ=255)

                                               tgtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTg                                         <  2:4549703/93‑1 (MQ=255)

                                               tgtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTg                                         <  2:3741019/93‑1 (MQ=255)

                                                gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTgg                                        <  1:7850308/93‑1 (MQ=255)

                                                gtgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTgg                                        <  2:7696988/93‑1 (MQ=255)

                                                 tgCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTggg                                       >  2:1521817/1‑93 (MQ=255)

                                                  gCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGc                                      <  1:6415259/93‑1 (MQ=255)

                                                  gCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGc                                      >  1:7727110/1‑93 (MQ=255)

                                                   cacaAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCa                                     >  1:2910071/1‑93 (MQ=255)

                                                    acaAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCaa                                    >  1:4255282/1‑93 (MQ=255)

                                                     caAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAAc                                   <  1:4734169/93‑1 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGCTTGGGCAAcca                                 >  2:2197015/1‑91 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTa                                 <  2:2257558/93‑1 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTa                                 >  1:7091121/1‑93 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTa                                 >  1:4766315/1‑93 (MQ=255)

                                                       aaaGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTa                                 <  2:7114349/93‑1 (MQ=255)

                                                        aaGGCTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTCAAAACTGGCACGCTCGCGGAGATTGGGCAACTAg                                <  2:2375522/93‑1 (MQ=255)

                                                         aGGTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGc                               >  2:5835206/1‑93 (MQ=255)

                                                          ggTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCt                              >  2:2054156/1‑93 (MQ=255)

                                                          ggTTACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCt                              >  1:101316/1‑93 (MQ=255)

                                                           gTTACGAGGTCATCGTTGAGCAACAAATCGCCCGCGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCACCTAGCTg                             <  2:5225378/93‑1 (MQ=255)

                                                            ttACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGa                            <  1:5412746/93‑1 (MQ=255)

                                                            ttACGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGa                            <  1:5966748/93‑1 (MQ=255)

                                                              aCGAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAtc                          <  2:5433207/93‑1 (MQ=255)

                                                                gAGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAtctc                        >  2:904605/1‑93 (MQ=255)

                                                                 aGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg                       >  2:5295979/1‑93 (MQ=255)

                                                                 aGGTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg                       <  1:7412692/93‑1 (MQ=255)

                                                                 aGGTCATCGTTGAGCAACAAATCGCCCACGAACGGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcg                       <  1:7604451/93‑1 (MQ=255)

                                                                  ggTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcgc                      >  2:3911129/1‑93 (MQ=255)

                                                                  ggTCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTcgc                      >  2:6468727/1‑93 (MQ=255)

                                                                    tCATCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCgg                    >  1:1882704/1‑93 (MQ=255)

                                                                     cATCGTTGAGCAACAAATCGCACACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGt                   <  1:3755021/93‑1 (MQ=255)

                                                                      aTCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTa                  <  2:551773/93‑1 (MQ=255)

                                                                       tCGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAg                 >  2:813988/1‑93 (MQ=255)

                                                                        cGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGt                <  2:4026533/93‑1 (MQ=255)

                                                                        cGTTGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGt                <  2:7177148/93‑1 (MQ=255)

                                                                           tGAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGt             >  1:2526178/1‑93 (MQ=255)

                                                                            gAGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGtt            <  2:7533020/93‑1 (MQ=255)

                                                                             aGCAACAAATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtg           <  2:3150857/93‑1 (MQ=255)

                                                                                aacaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGAACTCGCGGTAGTCGTtggtg        >  1:5013610/1‑93 (MQ=255)

                                                                                 acaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtgg       >  1:3126602/1‑93 (MQ=255)

                                                                                 acaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtgg       >  1:701110/1‑93 (MQ=255)

                                                                                 acaaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTtggtgg       >  1:4329362/1‑93 (MQ=255)

                                                                                  caaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc      <  2:1148319/93‑1 (MQ=255)

                                                                                  caaATCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc      >  1:144391/1‑93 (MQ=255)

                                                                                  caaATCGCCCACGAACTGCAACTGAAGAATGGGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGc      >  1:6783880/1‑93 (MQ=255)

                                                                                    aaTCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGa    <  1:3839289/93‑1 (MQ=255)

                                                                                      tCGCCCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACg  >  2:5021813/1‑93 (MQ=255)

ACGGCACCCCACTGCACTGACAACACATGAAATGCTCTACCGCTGGCTGTGCACAAAAGGTTACGAGGTCATCGTTGAGCAACAAATCGCTCACGAACTGCAACTGAAGAATGTGAAAACTGGCACGCTCGCGGAGATTGGGCAACTAGCTGATCTCGCGGTAGTCGTTGGTGGCGACG  >  NC_000913/2748893‑2749071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`