BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,759,957	A→G	T535T (ACT→ACC)	yfjK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,759,957	0	A	G	99.2%	801.3 / ‑4.2	255	T535T (ACT→ACC)	yfjK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (112/141); total (113/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 262 total aligned reads displayed.

GGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTA  >  NC_000913/2759868‑2760046

  cTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCa                                                                                      <  1:7517849/93‑1 (MQ=255)

   ttAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAg                                                                                     <  1:5072853/93‑1 (MQ=255)

    tAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGt                                                                                    <  1:5888687/93‑1 (MQ=255)

    tAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGt                                                                                    >  2:2479549/1‑93 (MQ=255)

       cAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGaa                                                                                 >  2:5567300/1‑93 (MQ=255)

        aGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGaaa                                                                                <  2:2951390/93‑1 (MQ=255)

          ggACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAaca                                                                              <  1:7740096/93‑1 (MQ=255)

            aCCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACt                                                                            <  1:1337892/93‑1 (MQ=255)

              cccATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTaa                                                                          <  1:2539610/93‑1 (MQ=255)

               ccATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAAc                                                                         >  1:6798368/1‑93 (MQ=255)

               ccATTGCCACCGAACGTCTGGGGAACCAGTCTGGCAAGGATCTTTGCAAAGTTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAAc                                                                         <  2:1919269/93‑1 (MQ=255)

                cATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACg                                                                        >  1:70247/1‑93 (MQ=255)

                 aTTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGa                                                                       <  1:2144055/93‑1 (MQ=255)

                 aTTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGa                                                                       >  2:3165944/1‑93 (MQ=255)

                  ttGCGACTGAACGTCTGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAg                                                                      <  2:6188259/93‑1 (MQ=255)

                  ttGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAg                                                                      <  2:1453977/93‑1 (MQ=255)

                   tGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAgg                                                                     <  2:3607166/93‑1 (MQ=255)

                   tGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAgg                                                                     <  2:2362709/93‑1 (MQ=255)

                     cGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGaa                                                                   <  2:5195019/93‑1 (MQ=255)

                      gACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGaaa                                                                  >  2:4010615/1‑93 (MQ=255)

                      gACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGaaa                                                                  >  2:7910027/1‑93 (MQ=255)

                       aCTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGaaaa                                                                 <  1:7223724/93‑1 (MQ=255)

                       aCTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGaaaa                                                                 >  2:5684811/1‑93 (MQ=255)

                         tGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAAtt                                                               >  1:885224/1‑93 (MQ=255)

                         tGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAAtt                                                               >  2:755366/1‑93 (MQ=255)

                         tGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAAtt                                                               >  1:5257038/1‑93 (MQ=255)

                         tGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAAtt                                                               >  1:601983/1‑93 (MQ=255)

                               tATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAGAATTCGTTAt                                                         >  1:5352779/1‑93 (MQ=255)

                               tATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTAt                                                         >  1:7638474/1‑93 (MQ=255)

                                aTGGGGAACCAGTCTGGCAATGATCTTTGCAGAGGTCTTGAGGAAGTTTGTCGCTTGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATc                                                        <  2:5353454/93‑1 (MQ=255)

                                  ggggAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAAAGAGGAAAATTCATTATCGt                                                      >  1:7303225/1‑93 (MQ=255)

                                    ggAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTcc                                                    >  2:4580823/1‑93 (MQ=255)

                                     gAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCca                                                   <  1:4915063/93‑1 (MQ=255)

                                     gAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCca                                                   <  2:6685153/93‑1 (MQ=255)

                                      aaCCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcat                                                  >  2:2723742/1‑93 (MQ=255)

                                       aCCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcatc                                                 <  1:7428347/93‑1 (MQ=255)

                                        ccAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcatca                                                <  2:2113901/93‑1 (MQ=255)

                                        ccAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcatca                                                <  2:6812409/93‑1 (MQ=255)

                                        ccAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcatca                                                <  2:3348963/93‑1 (MQ=255)

                                        ccAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcatca                                                >  1:2742388/1‑93 (MQ=255)

                                         cAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCcatcat                                               <  2:5461235/93‑1 (MQ=255)

                                          aGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCatcatc                                              >  2:394312/1‑93 (MQ=255)

                                          aGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCatcatc                                              <  1:7163679/93‑1 (MQ=255)

                                          aGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCatcatc                                              >  1:2979161/1‑93 (MQ=255)

                                           gTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCAtct                                             <  1:305204/93‑1 (MQ=255)

                                              tGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGa                                          >  2:1535357/1‑93 (MQ=255)

                                              tGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGa                                          >  1:3727453/1‑93 (MQ=255)

                                                 cAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACa                                       <  2:6351335/93‑1 (MQ=255)

                                                 cAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACa                                       >  1:6246489/1‑93 (MQ=255)

                                                 cAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACa                                       <  1:5828069/93‑1 (MQ=255)

                                                  aaTGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACAt                                      >  2:6229140/1‑93 (MQ=255)

                                                   aTGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATg                                     >  2:7446747/1‑93 (MQ=255)

                                                   aTGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATg                                     >  1:4420122/1‑93 (MQ=255)

                                                    tGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGc                                    >  2:104148/1‑93 (MQ=255)

                                                    tGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGc                                    >  1:1747628/1‑93 (MQ=255)

                                                    tGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGc                                    <  2:230175/93‑1 (MQ=255)

                                                     gATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCt                                   >  2:4839503/1‑93 (MQ=255)

                                                      aTCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAGAATTCGTTATCGTCCATCATCTCGAAGATGCtt                                  >  2:4136196/1‑93 (MQ=255)

                                                      aTCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCtt                                  >  2:480134/1‑93 (MQ=255)

                                                      aTCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCtt                                  <  2:462206/93‑1 (MQ=255)

                                                       tCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCttt                                 >  1:2678865/1‑93 (MQ=255)

                                                       tCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCttt                                 >  1:1063291/1‑93 (MQ=255)

                                                       tCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCttt                                 >  1:2848820/1‑93 (MQ=255)

                                                       tCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGGCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCttt                                 >  2:6531645/1‑93 (MQ=255)

                                                         tttGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgc                               >  1:337654/1‑93 (MQ=255)

                                                          ttGCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgca                              <  2:687089/93‑1 (MQ=255)

                                                            gCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgcagc                            <  1:409719/93‑1 (MQ=255)

                                                            gCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgcagc                            <  2:1227409/93‑1 (MQ=255)

                                                            gCAAAGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgcagc                            <  2:6561825/93‑1 (MQ=255)

                                                              aaaGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCAt                          >  2:5198462/1‑93 (MQ=255)

                                                              aaaGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCAt                          >  2:6264057/1‑93 (MQ=255)

                                                                aGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCAttt                        <  1:3095762/93‑1 (MQ=255)

                                                                aGGTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCAttt                        <  1:6682701/93‑1 (MQ=255)

                                                                 ggTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTc                       <  1:4065445/93‑1 (MQ=255)

                                                                 ggTCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTc                       >  1:2322456/1‑93 (MQ=255)

                                                                   tCTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTTGAACATGCTTTGCAGCATTTCGa                     >  2:4277958/1‑93 (MQ=255)

                                                                    cTTGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGaa                    <  2:712102/93‑1 (MQ=255)

                                                                     ttGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAAc                   <  1:362128/93‑1 (MQ=255)

                                                                     ttGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAAc                   >  2:3838132/1‑93 (MQ=255)

                                                                     ttGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAAc                   >  1:235014/1‑93 (MQ=255)

                                                                      tGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACt                  <  2:2838382/93‑1 (MQ=255)

                                                                      tGAGGAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACt                  <  2:6128007/93‑1 (MQ=255)

                                                                         ggAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTACATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCt               >  1:6280340/1‑93 (MQ=255)

                                                                          gAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTc              <  2:5998816/93‑1 (MQ=255)

                                                                          gAAGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTc              <  1:3198749/93‑1 (MQ=255)

                                                                           aaGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCg             >  2:1754392/1‑93 (MQ=255)

                                                                           aaGTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCg             <  2:515541/93‑1 (MQ=255)

                                                                             gTTTGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGac           >  1:1018081/1‑93 (MQ=255)

                                                                              tttGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGaca          <  2:7682856/93‑1 (MQ=255)

                                                                              tttGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGaca          >  1:1514563/1‑93 (MQ=255)

                                                                              tttGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGaca          <  2:1957849/93‑1 (MQ=255)

                                                                               ttGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGacac         <  1:5289561/93‑1 (MQ=255)

                                                                                tGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACg        >  1:3895719/1‑93 (MQ=255)

                                                                                tGTCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACg        <  2:2827194/93‑1 (MQ=255)

                                                                                  tCGCGGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGa      <  2:7298730/93‑1 (MQ=255)

                                                                                   cgcgGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGaa     <  2:5641752/93‑1 (MQ=255)

                                                                                     cgGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGt   <  1:4568822/93‑1 (MQ=255)

                                                                                     cgGGGGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGt   <  1:4498313/93‑1 (MQ=255)

                                                                                      ggggGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTa  <  1:1910451/93‑1 (MQ=255)

                                                                                      ggggGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTa  <  2:2534483/93‑1 (MQ=255)

GGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTA  >  NC_000913/2759868‑2760046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`