BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,798,359	A→G	T65A (ACT→GCT)	b2672 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,798,359	0	A	G	99.7%	927.1 / NA	300	T65A (ACT→GCT)	b2672	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (150/149); minor base C (0/1); total (150/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

TGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCT  >  NC_000913/2798269‑2798448

 ggggCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCACAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTc                                                                                        >  2:4677201/1‑93 (MQ=255)

   ggCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGTGCGAATGCATGGTCGTGCTcgc                                                                                      <  2:4534564/93‑1 (MQ=255)

     cAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGt                                                                                    >  1:6693113/1‑93 (MQ=255)

      aGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTc                                                                                   <  1:6529864/93‑1 (MQ=255)

       gCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTcc                                                                                  >  1:5920125/1‑93 (MQ=255)

       gCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTcc                                                                                  >  2:6426385/1‑93 (MQ=255)

       gCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTcc                                                                                  <  1:3279226/93‑1 (MQ=255)

         gACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCac                                                                                >  2:1817081/1‑92 (MQ=255)

         gACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAg                                                                                <  2:1028731/93‑1 (MQ=255)

          aCGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGc                                                                               >  2:7060141/1‑93 (MQ=255)

          aCGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGc                                                                               >  1:3156924/1‑93 (MQ=255)

             ccAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGGGCGAAGGCATGGTCGTGCTCGCGTCGAGCAAg                                                                            >  1:1645803/1‑93 (MQ=255)

              cAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGc                                                                           <  1:3880226/93‑1 (MQ=255)

               aaaGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGcc                                                                          <  2:48763/93‑1 (MQ=255)

                 aGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCcgc                                                                        >  1:7368842/1‑93 (MQ=255)

                 aGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCcgc                                                                        >  1:4567138/1‑93 (MQ=255)

                   gggAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCcgcgc                                                                      <  2:7679154/93‑1 (MQ=255)

                   gggAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCcgcgc                                                                      <  1:3403866/93‑1 (MQ=255)

                     gAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCcgcgcgc                                                                    >  2:5191761/1‑93 (MQ=255)

                      aaGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCcgcgcgcg                                                                   >  1:1866140/1‑93 (MQ=255)

                       aGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGa                                                                  <  1:1037533/93‑1 (MQ=255)

                        gCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGAt                                                                 >  1:4188942/1‑93 (MQ=255)

                          tGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGc                                                               >  2:2341034/1‑93 (MQ=255)

                          tGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGc                                                               <  1:5055859/93‑1 (MQ=255)

                             aGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGt                                                            <  1:3958997/93‑1 (MQ=255)

                             aGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGt                                                            >  2:2594672/1‑93 (MQ=255)

                                cgcacgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTgg                                                         >  1:1395109/1‑93 (MQ=255)

                                  cacgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCt                                                       >  1:3035888/1‑93 (MQ=255)

                                   acgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTg                                                      <  2:1314800/93‑1 (MQ=255)

                                    cgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgc                                                     <  2:4961090/93‑1 (MQ=255)

                                    cgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgc                                                     >  1:2737630/1‑93 (MQ=255)

                                    cgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgc                                                     <  1:1213761/93‑1 (MQ=255)

                                     gcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgcg                                                    <  2:3567570/93‑1 (MQ=255)

                                     gcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgcg                                                    >  1:1900506/1‑93 (MQ=255)

                                        gTAAGGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATTGTCGTGCTCGCGTCCCGCAAGCCGCGCGCGCTGCCGTTGGCTGCGCgg                                                 >  1:4291107/1‑93 (MQ=255)

                                        gTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCgg                                                 >  2:5747372/1‑93 (MQ=255)

                                        gTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCgg                                                 >  1:6847897/1‑93 (MQ=255)

                                         tAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGa                                                >  1:6267333/1‑93 (MQ=255)

                                          aaaGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGAt                                               <  2:5522515/93‑1 (MQ=255)

                                           aaGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGAtt                                              >  2:6516532/1‑93 (MQ=255)

                                            aGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAACGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTc                                             >  2:2927761/1‑93 (MQ=255)

                                             gCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCt                                            >  1:6469674/1‑93 (MQ=255)

                                             gCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCt                                            >  1:6975643/1‑93 (MQ=255)

                                               tCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCttt                                          >  2:1144290/1‑93 (MQ=255)

                                               tCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCttt                                          <  1:193695/93‑1 (MQ=255)

                                                cAGGCATTGCTGAAAGAACCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCtttt                                         <  2:5383172/93‑1 (MQ=255)

                                                cAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCtttt                                         <  1:1773782/93‑1 (MQ=255)

                                                cAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCtttt                                         >  1:5460630/1‑93 (MQ=255)

                                                 aGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTg                                        >  1:7600114/1‑93 (MQ=255)

                                                 aGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTg                                        >  2:7881889/1‑93 (MQ=255)

                                                 aGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTg                                        <  2:4257052/93‑1 (MQ=255)

                                                 aGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTg                                        <  2:3325602/93‑1 (MQ=255)

                                                  ggCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGt                                       <  2:1617280/93‑1 (MQ=255)

                                                  ggCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGt                                       >  1:7833507/1‑93 (MQ=255)

                                                   gCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGtt                                      <  2:689936/93‑1 (MQ=255)

                                                     aTTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGGCGTTGGCTGCGCGGATTCTTTTGTTct                                    >  1:840187/1‑92 (MQ=255)

                                                     aTTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCg                                    <  2:2244497/93‑1 (MQ=255)

                                                       tGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTg                                  <  2:6039194/93‑1 (MQ=255)

                                                       tGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTg                                  >  1:2886748/1‑93 (MQ=255)

                                                        gCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGa                                 <  2:3920305/93‑1 (MQ=255)

                                                        gCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGa                                 <  1:6111902/93‑1 (MQ=255)

                                                          tGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGGTGCGCGGATTCTTTTGTTCGTGaaa                               <  2:7340237/93‑1 (MQ=255)

                                                          tGAAAGAAACCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGaaa                               <  1:573008/93‑1 (MQ=255)

                                                           gaaagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAg                              >  1:5680202/1‑93 (MQ=255)

                                                           gaaagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAg                              <  1:1294480/93‑1 (MQ=255)

                                                            aaagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGa                             >  2:6233235/1‑93 (MQ=255)

                                                            aaagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGa                             <  1:5793490/93‑1 (MQ=255)

                                                            aaagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGa                             <  2:7564573/93‑1 (MQ=255)

                                                             aagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAc                            <  1:2589272/93‑1 (MQ=255)

                                                             aagaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAc                            <  2:2392117/93‑1 (MQ=255)

                                                               gaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAccc                          >  2:3612356/1‑93 (MQ=255)

                                                               aaaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAccc                          <  1:2324840/92‑1 (MQ=255)

                                                                aaaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCt                         <  1:3555408/93‑1 (MQ=255)

                                                                 aaCCAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTg                        <  1:7931876/93‑1 (MQ=255)

                                                                 aaCCAGGGCGCGAATGCAGGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTg                        <  1:5333162/93‑1 (MQ=255)

                                                                   ccAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGgt                      <  1:6358719/93‑1 (MQ=255)

                                                                   ccAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGgt                      <  2:3191392/93‑1 (MQ=255)

                                                                   ccAGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGgt                      >  2:7191169/1‑93 (MQ=255)

                                                                     aGGGCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGgtgt                    >  1:2009807/1‑93 (MQ=255)

                                                                      gggCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTa                   <  2:7468111/93‑1 (MQ=255)

                                                                      gggCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTa                   <  2:7377388/93‑1 (MQ=255)

                                                                       ggCGCGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAg                  >  1:1677260/1‑93 (MQ=255)

                                                                        gcgcGAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGc                 <  2:6808303/93‑1 (MQ=255)

                                                                           cgAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTg              >  2:272756/1‑93 (MQ=255)

                                                                            gAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTgg             >  2:2990242/1‑93 (MQ=255)

                                                                            gAATGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTgg             >  2:6207898/1‑93 (MQ=255)

                                                                             aaTGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTggg            <  2:2001742/93‑1 (MQ=255)

                                                                             aaTGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTggg            >  2:4612577/1‑93 (MQ=255)

                                                                              aTGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGt           >  2:6342469/1‑93 (MQ=255)

                                                                              aTGCATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGt           >  2:151196/1‑93 (MQ=255)

                                                                                 cATGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTAca        <  2:4415587/93‑1 (MQ=255)

                                                                                   tGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTAcagc      <  2:2040975/93‑1 (MQ=255)

                                                                                   tGGTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTAcagc      <  2:5930872/93‑1 (MQ=255)

                                                                                     gTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTAcagcag    <  1:7112391/93‑1 (MQ=255)

                                                                                     gTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTAcagcag    <  1:4702380/93‑1 (MQ=255)

                                                                                     gTCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTAcagcag    >  2:3079267/1‑93 (MQ=255)

                                                                                      tCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACagcagc   <  1:2176585/93‑1 (MQ=255)

                                                                                      tCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACagcagc   <  2:4390389/93‑1 (MQ=255)

                                                                                      tCGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACagcagc   <  2:779691/93‑1 (MQ=255)

                                                                                      gcGTGCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACagcagc   <  1:2610686/92‑1 (MQ=255)

TGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCT  >  NC_000913/2798269‑2798448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`