BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	302,564	A→G	Y117C (TAT→TGT)	yagU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302,564	0	A	G	99.6%	883.5 / ‑4.9	280	Y117C (TAT→TGT)	yagU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (149/130); total (149/131)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 298 total aligned reads displayed.

CCAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGATTT  >  NC_000913/302474‑302653

ccAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGgttgtt                                                                                         <  2:1611831/93‑1 (MQ=255)

ccAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGgttgtt                                                                                         >  1:6737138/1‑93 (MQ=255)

ccAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGgttgtt                                                                                         >  1:3412350/1‑93 (MQ=255)

  aaTGGGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTtgttgt                                                                                       >  2:5044021/1‑93 (MQ=255)

  aaTGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTtgttgt                                                                                       >  1:2509955/1‑93 (MQ=255)

   aTGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTtgtg                                                                                      <  1:7430415/93‑1 (MQ=255)

    tGCGGCTGTTTATTCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTtgtgt                                                                                     <  1:1254015/93‑1 (MQ=255)

    tGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTtgtgt                                                                                     >  1:5081096/1‑93 (MQ=255)

    tGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTtgtgt                                                                                     >  2:5277334/1‑93 (MQ=255)

     gCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTtgtgtg                                                                                    <  2:5520590/93‑1 (MQ=255)

       tgttGTTTATTCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTTTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGt                                                                                  <  2:1317188/90‑1 (MQ=255)

       ggCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGt                                                                                  <  1:7633918/93‑1 (MQ=255)

       ggCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGt                                                                                  >  2:6485047/1‑93 (MQ=255)

        gCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTc                                                                                 <  2:4233678/93‑1 (MQ=255)

        gCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTc                                                                                 >  1:3369594/1‑93 (MQ=255)

          tGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGc                                                                               <  2:3355405/93‑1 (MQ=255)

          tGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGc                                                                               >  2:3725467/1‑93 (MQ=255)

           gTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCt                                                                              <  2:2516237/93‑1 (MQ=255)

           gTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCt                                                                              >  2:3951578/1‑93 (MQ=255)

            tttATACCTTTGCCGGGCATGTCTTTAACAGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTg                                                                             >  2:4840783/1‑93 (MQ=255)

             ttATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGa                                                                            >  2:4297486/1‑93 (MQ=255)

               ataCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAg                                                                          <  1:4361456/93‑1 (MQ=255)

                 aCCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTa                                                                        <  2:6664604/93‑1 (MQ=255)

                  ccTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTAt                                                                       <  1:240586/93‑1 (MQ=255)

                   cTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTAtt                                                                      >  2:6177246/1‑93 (MQ=255)

                    tttGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTAttt                                                                     >  1:4795345/1‑93 (MQ=255)

                      tGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTcc                                                                   >  1:5609051/1‑93 (MQ=255)

                        cctggCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCaa                                                                 <  2:7057074/90‑1 (MQ=255)

                         cGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCaaa                                                                >  2:7817103/1‑93 (MQ=255)

                           ggCATTTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCTGTTGTTGTGTGGTTGCTTATGTATTTCCaaaaa                                                              >  1:7044041/1‑93 (MQ=255)

                           ggCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCaaaaa                                                              >  2:4478520/1‑93 (MQ=255)

                           ggCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCaaaaa                                                              >  1:5938879/1‑93 (MQ=255)

                            gCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAAt                                                             <  2:472624/93‑1 (MQ=255)

                            gCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAAt                                                             <  1:639223/93‑1 (MQ=255)

                            gCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAAt                                                             >  1:5465196/1‑93 (MQ=255)

                            gCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGAAAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAAt                                                             >  2:198648/1‑93 (MQ=255)

                              aTGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTa                                                           >  1:471211/1‑93 (MQ=255)

                                gTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTaaa                                                         <  2:4201121/93‑1 (MQ=255)

                                gTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTaaa                                                         <  1:7856670/93‑1 (MQ=255)

                                gTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTaaa                                                         >  2:1724206/1‑93 (MQ=255)

                                  cTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAAct                                                       <  2:2180972/93‑1 (MQ=255)

                                    ttAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAActct                                                     <  2:52578/93‑1 (MQ=255)

                                     tAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTg                                                    <  1:1952028/93‑1 (MQ=255)

                                      aaCTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTgg                                                   >  2:283890/1‑93 (MQ=255)

                                        cTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCa                                                 >  1:3218488/1‑93 (MQ=255)

                                         tGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAg                                                >  2:857698/1‑93 (MQ=255)

                                         tGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAg                                                >  1:56831/1‑93 (MQ=255)

                                         tGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTACAAAAATTAAACTCTGGCAg                                                >  2:6198182/1‑93 (MQ=255)

                                          gggTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAgg                                               <  1:4150477/93‑1 (MQ=255)

                                          gggTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAgg                                               >  1:7733838/1‑93 (MQ=255)

                                           ggTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAggg                                              >  2:5635800/1‑93 (MQ=255)

                                           ggTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAggg                                              >  1:5895533/1‑93 (MQ=255)

                                            gTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGc                                             >  2:3482041/1‑93 (MQ=255)

                                             ttGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCt                                            >  1:5123889/1‑93 (MQ=255)

                                              tGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCtt                                           >  2:1265747/1‑93 (MQ=255)

                                               ggTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTa                                          >  1:1185099/1‑93 (MQ=255)

                                               ggTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTa                                          >  2:812722/1‑93 (MQ=255)

                                                gtgtTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTAc                                         >  1:2267597/1‑93 (MQ=255)

                                                gtgtTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTAc                                         <  2:4114402/93‑1 (MQ=255)

                                                    tACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGc                                     <  1:1701448/93‑1 (MQ=255)

                                                     aCGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTgcag                                    <  1:3950292/93‑4 (MQ=255)

                                                     aCGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCa                                    <  2:3043995/93‑1 (MQ=255)

                                                      cGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAg                                   >  2:6350961/1‑93 (MQ=255)

                                                       gCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAgg                                  >  2:1438859/1‑93 (MQ=255)

                                                       gCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAgg                                  <  2:2371817/93‑1 (MQ=255)

                                                       ccactTTATTTTTTCGATAGTGTTTGTTGTCGTTTGTTGTGTGGTCGCTAAAGTATTTCAAAAAATTAACCTCGGGCAGGGCTTACTGGCAgg                                  <  2:6989071/92‑1 (MQ=255)

                                                         acaTTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTg                                >  2:6845739/1‑93 (MQ=255)

                                                          caTTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGc                               <  1:869701/93‑1 (MQ=255)

                                                           aTTATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCt                              <  1:6563267/93‑1 (MQ=255)

                                                            ttATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCtt                             >  2:75177/1‑93 (MQ=255)

                                                             tATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCttt                            >  2:5454304/1‑93 (MQ=255)

                                                             tATCTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCttt                            >  1:58153/1‑93 (MQ=255)

                                                                cTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGc                         >  1:3627843/1‑93 (MQ=255)

                                                                cTTTTCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGc                         >  2:4081208/1‑93 (MQ=255)

                                                                 ttttCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGcc                        >  1:5379711/1‑93 (MQ=255)

                                                                 ttttCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGcc                        >  1:6205056/1‑93 (MQ=255)

                                                                  tttCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGccc                       <  1:4948565/93‑1 (MQ=255)

                                                                   ttCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCa                      >  1:5233056/1‑93 (MQ=255)

                                                                    tCGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTTGcccca                     >  2:1057912/1‑91 (MQ=255)

                                                                     cGATAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAAc                    <  1:6865109/93‑1 (MQ=255)

                                                                       aTAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGGGCTTTAGCCCAACtt                  >  1:7279501/1‑93 (MQ=255)

                                                                        tAGTGTTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACttt                 >  2:7543046/1‑93 (MQ=255)

                                                                          gtgtTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACttttt               >  2:5194174/1‑93 (MQ=255)

                                                                          gtgtTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACttttt               <  1:792049/93‑1 (MQ=255)

                                                                          gtgtTTGCTGTCGGTTGTTGTGTGGCCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACttttt               <  2:3205551/93‑1 (MQ=255)

                                                                           tgtTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTg              >  2:3128281/1‑93 (MQ=255)

                                                                            gtTTGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGt             >  1:2834526/1‑93 (MQ=255)

                                                                             tttGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGGCCAACTTTTTGtt            >  1:4545512/1‑93 (MQ=255)

                                                                             tttGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAAATTTTTGtt            >  2:3797367/1‑93 (MQ=255)

                                                                               tGCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCa          <  2:4808016/93‑1 (MQ=255)

                                                                                gCTGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCat         >  2:5757057/1‑93 (MQ=255)

                                                                                  tGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCCACTTTTTGTTCatat       >  2:72123/1‑93 (MQ=255)

                                                                                  tGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCatat       >  1:4674576/1‑93 (MQ=255)

                                                                                  tGTCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCatat       >  1:4855315/1‑93 (MQ=255)

                                                                                    tCGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGa     <  1:1047097/93‑1 (MQ=255)

                                                                                     cGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGAt    <  2:6621094/93‑1 (MQ=255)

                                                                                     cGGTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGAt    <  2:5310636/93‑1 (MQ=255)

                                                                                      ggTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGAtt   <  2:5944314/93‑1 (MQ=255)

                                                                                      ggTTGTTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGAtt   <  2:2719257/93‑1 (MQ=255)

                                                                                       gttgttGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGAttt  >  1:3811092/1‑93 (MQ=255)

CCAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAACTGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTCGCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAACTTTTTGTTCATATGATTT  >  NC_000913/302474‑302653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`