BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,818,582	A→G	N484N (AAT→AAC)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,582	0	A	G	99.7%	1026.8 / ‑5.4	326	N484N (AAT→AAC)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (172/152); total (174/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 333 total aligned reads displayed.

GCCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCATTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATGC  >  NC_000913/2818492‑2818671

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCTCAGCTTCCTGGCCTGCGtt                                                                                         >  2:7034927/1‑93 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                         >  2:4654401/1‑93 (MQ=255)

gcCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGtt                                                                                         >  1:1571784/1‑93 (MQ=255)

 ccTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTg                                                                                        >  2:7807318/1‑93 (MQ=255)

 ccTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTg                                                                                        >  1:3165244/1‑93 (MQ=255)

  cTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGa                                                                                       >  1:552949/1‑93 (MQ=255)

  cTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGa                                                                                       <  1:5979408/93‑1 (MQ=255)

   tttCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGAt                                                                                      >  1:6456881/1‑93 (MQ=255)

   tttCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGAt                                                                                      >  2:2445569/1‑93 (MQ=255)

    ttCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATg                                                                                     >  1:2670442/1‑93 (MQ=255)

     tCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATgg                                                                                    >  1:4382839/1‑93 (MQ=255)

        gTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCAt                                                                                 >  2:2611341/1‑93 (MQ=255)

         ttCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATc                                                                                <  1:5080218/93‑1 (MQ=255)

         ttCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATc                                                                                >  1:2638276/1‑93 (MQ=255)

         ttCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGACTGCGTTGATGGCATc                                                                                >  1:7839240/1‑93 (MQ=255)

          tCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCa                                                                               <  1:7618556/93‑1 (MQ=255)

                ttATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCt                                                                         >  2:1949991/1‑93 (MQ=255)

                   tCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTa                                                                      <  1:2859037/93‑1 (MQ=255)

                   tCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTa                                                                      <  2:5118955/93‑1 (MQ=255)

                     gCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTAcc                                                                    >  2:2328107/1‑93 (MQ=255)

                      ccAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCa                                                                   <  2:6906699/93‑1 (MQ=255)

                       cAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCAt                                                                  >  2:1641046/1‑93 (MQ=255)

                       cAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCAt                                                                  <  1:6748532/93‑1 (MQ=255)

                        aaCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATc                                                                 >  1:2291635/1‑93 (MQ=255)

                         aCCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATca                                                                <  2:3560324/93‑1 (MQ=255)

                            tGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATcaaca                                                             >  2:6546483/1‑93 (MQ=255)

                            tGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATcaaca                                                             >  2:3238933/1‑93 (MQ=255)

                             gACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATcaacaa                                                            >  2:2276762/1‑93 (MQ=255)

                              aCCGGCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCACCCGCTTTACCATCaacaaa                                                           <  2:4140926/93‑1 (MQ=255)

                              aCCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCGTCAACCGCTTTACCATCaacaaa                                                           >  2:5650243/1‑93 (MQ=255)

                                 gccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAg                                                        <  2:7682220/93‑1 (MQ=255)

                                  ccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACACACAgc                                                       >  1:1305500/1‑93 (MQ=255)

                                  ccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgc                                                       <  2:7582548/93‑1 (MQ=255)

                                  ccgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgc                                                       >  2:2848260/1‑93 (MQ=255)

                                   cgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgcg                                                      >  1:4727775/1‑93 (MQ=255)

                                   cgGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgcg                                                      >  2:4281863/1‑93 (MQ=255)

                                    ggATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgcgc                                                     >  2:1807675/1‑93 (MQ=255)

                                    ggATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAgcgc                                                     >  1:7366247/1‑93 (MQ=255)

                                     gATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCa                                                    <  1:5328108/93‑1 (MQ=255)

                                      aTTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAg                                                   <  1:356086/93‑1 (MQ=255)

                                       ttCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGt                                                  >  1:3043032/1‑93 (MQ=255)

                                       ttCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGt                                                  <  1:5232860/93‑1 (MQ=255)

                                        tCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTc                                                 <  2:6362315/93‑1 (MQ=255)

                                         ccGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCa                                                >  2:4840966/1‑93 (MQ=255)

                                         ccGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCa                                                >  2:1708309/1‑93 (MQ=255)

                                         ccGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCa                                                >  1:2258668/1‑93 (MQ=255)

                                           gCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACt                                              >  2:5239011/1‑93 (MQ=255)

                                           gCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACt                                              >  1:2347760/1‑93 (MQ=255)

                                            cATAGAATGGCGTTTGATCCAGCACGACTACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACtt                                             <  1:4632366/93‑1 (MQ=255)

                                             aTAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACttt                                            >  1:542481/1‑93 (MQ=255)

                                             aTAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACttt                                            >  1:1533007/1‑93 (MQ=255)

                                             aTAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACttt                                            <  1:6043220/93‑1 (MQ=255)

                                              tAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTg                                           >  1:2903956/1‑93 (MQ=255)

                                                gAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGcc                                         >  1:2089659/1‑93 (MQ=255)

                                                 aaTGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCg                                        >  2:1018565/1‑93 (MQ=255)

                                                   tGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGtt                                      >  2:978065/1‑93 (MQ=255)

                                                   tGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGtt                                      <  2:735116/93‑1 (MQ=255)

                                                       gTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGt                                  >  2:6061234/1‑93 (MQ=255)

                                                        tttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGtt                                 >  2:1887090/1‑93 (MQ=255)

                                                        tttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGtt                                 >  1:1934531/1‑93 (MQ=255)

                                                        tttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGtt                                 >  2:7134713/1‑93 (MQ=255)

                                                        tttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGtt                                 >  2:5830950/1‑93 (MQ=255)

                                                         ttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTc                                <  2:7221883/93‑1 (MQ=255)

                                                         ttGATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTc                                >  1:1964338/1‑93 (MQ=255)

                                                          tGAGCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTcc                               <  1:443713/93‑1 (MQ=255)

                                                           gATCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCa                              <  1:889901/93‑1 (MQ=255)

                                                             tCCAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGa                            >  1:6905319/1‑93 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                           <  1:4893450/93‑1 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                           <  1:5014827/93‑1 (MQ=255)

                                                              ccAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCCCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGAt                           <  2:5420518/93‑1 (MQ=255)

                                                               cAGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATg                          <  1:1447564/93‑1 (MQ=255)

                                                                aGCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATgg                         <  1:3214002/93‑1 (MQ=255)

                                                                 gCACGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGt                        <  1:7779283/93‑1 (MQ=255)

                                                                   aCGACCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCa                      <  1:584139/93‑1 (MQ=255)

                                                                      aCCACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAg                   <  2:5006932/93‑1 (MQ=255)

                                                                       ccACAGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGc                  <  1:3306220/93‑1 (MQ=255)

                                                                        cacaGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGcc                 <  2:3444131/93‑1 (MQ=255)

                                                                         acaGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCt                <  1:657643/93‑1 (MQ=255)

                                                                         acaGCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCt                >  2:7462395/1‑93 (MQ=255)

                                                                            gCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTa             >  2:3602271/1‑93 (MQ=255)

                                                                            gCTTCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTa             <  1:1012509/93‑1 (MQ=255)

                                                                              ttCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTaaa           >  1:2439803/1‑93 (MQ=255)

                                                                              ttCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTaaa           <  2:2662524/93‑1 (MQ=255)

                                                                               tgcTGGCCTGCATTGATGGCATCAACCGCTTTACCATCAACAAACAGAGCAGTCACTTTGCCGTTTAGTTCCAGATTGTCATAGCCTTTAAAt          >  2:1328338/3‑93 (MQ=255)

                                                                               tCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAt          >  2:6843081/1‑93 (MQ=255)

                                                                               tCCTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTCTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAt          >  2:594461/1‑93 (MQ=255)

                                                                                ccTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAAtt         >  1:3992757/1‑93 (MQ=255)

                                                                                 cTGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTc        >  2:6232712/1‑93 (MQ=255)

                                                                                  tGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCa       <  1:1106455/93‑1 (MQ=255)

                                                                                  tGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCa       >  1:4826593/1‑93 (MQ=255)

                                                                                  tGGCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCa       >  2:4490850/1‑93 (MQ=255)

                                                                                  tGGCCTGCGTTGATGGCACCAACCGCTTTACCATCAACAAACAGCGCAGCCACTTTGCCGTTCAGTTCCAGATGGCCATAGCCTTTAAATTCa       >  2:7911880/1‑93 (MQ=255)

                                                                                    gCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGa     <  1:7188632/93‑1 (MQ=255)

                                                                                    gCCTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGa     <  1:7710937/93‑1 (MQ=255)

                                                                                    gCCTGCGGTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGa     <  1:350469/93‑1 (MQ=255)

                                                                                     ccTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGAt    <  2:1263812/93‑1 (MQ=255)

                                                                                     ccTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGAt    <  1:7232538/93‑1 (MQ=255)

                                                                                      cTGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATg   <  2:3126518/93‑1 (MQ=255)

                                                                                       tGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATGc  <  2:2152956/93‑1 (MQ=255)

                                                                                       tGCGTTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATGc  >  1:1662808/1‑93 (MQ=255)

GCCTTTCAGTTCGCCTTTATCGCCAACCTGACCGCCGGATTCCGCATAGAATGGCGTTTGATCCAGCACGACCACAGCTTCCTGGCCTGCATTGATGGCATCAACCGCTTTACCATCAACAAACAGCGCAGTCACTTTGCCGTTCAGTTCCAGATGGTCATAGCCTTTAAATTCAGATGC  >  NC_000913/2818492‑2818671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`