BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,549	T→C	E498G (GAA→GGA)	yagW ←	putative receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,549	0	T	C	99.2%	819.9 / ‑3.6	262	E498G (GAA→GGA)	yagW	putative receptor
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (127/133); total (128/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 275 total aligned reads displayed.

AACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTT  >  NC_000913/304459‑304638

 aCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGc                                                                                        >  2:1773071/1‑93 (MQ=255)

   cAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCa                                                                                      <  2:678897/93‑1 (MQ=255)

   cAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCa                                                                                      >  1:5750430/1‑93 (MQ=255)

   cAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCa                                                                                      <  1:868886/93‑1 (MQ=255)

     gCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCGGCCagg                                                                                    >  1:6201790/1‑92 (MQ=255)

     gCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCaga                                                                                    >  2:3899268/1‑93 (MQ=255)

       cGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAgaga                                                                                  <  2:1892846/93‑1 (MQ=255)

        gTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGat                                                                                 >  1:969211/1‑93 (MQ=255)

        gTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGat                                                                                 <  2:2849723/93‑1 (MQ=255)

         ttATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGata                                                                                >  1:5080287/1‑93 (MQ=255)

         ttATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGata                                                                                <  1:3080359/93‑1 (MQ=255)

          tATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGatat                                                                               <  1:250434/93‑1 (MQ=255)

          tATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGatat                                                                               >  2:3036678/1‑93 (MQ=255)

           atcatcTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATc                                                                              <  1:2305782/93‑1 (MQ=255)

             catcTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAg                                                                            <  1:5207334/93‑1 (MQ=255)

             catcTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAg                                                                            >  2:1281338/1‑93 (MQ=255)

             catcTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAg                                                                            >  2:6400412/1‑93 (MQ=255)

               tcTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTc                                                                          <  1:2925058/93‑1 (MQ=255)

                cTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTcc                                                                         <  2:6697736/93‑1 (MQ=255)

                  aCCTTACGCAGCGAAATGGCGTTATCCATTTGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCAc                                                                       <  1:812077/93‑1 (MQ=255)

                  aCCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCAc                                                                       <  1:598630/93‑1 (MQ=255)

                   ccGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACg                                                                      >  2:6095932/1‑93 (MQ=255)

                    cGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACgg                                                                     >  1:6472497/1‑93 (MQ=255)

                    cGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACgg                                                                     >  2:5729233/1‑93 (MQ=255)

                     gTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTTCACGgt                                                                    >  2:5487012/1‑93 (MQ=255)

                     gTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGgt                                                                    <  1:710904/93‑1 (MQ=255)

                      tACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGgtg                                                                   <  1:1690519/93‑1 (MQ=255)

                         gCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTgg                                                                <  2:1999335/93‑1 (MQ=255)

                         gCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTgg                                                                <  2:909384/93‑1 (MQ=255)

                         gCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCACCACTCCGCCAGAGATATCAGTCCACGGTGTgg                                                                >  2:7094579/1‑93 (MQ=255)

                           aGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTc                                                              <  1:4409900/93‑1 (MQ=255)

                            gCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCCCTCCGCCAGAGATATCAGTCCACGGTGTGGTCa                                                             <  2:5835129/93‑1 (MQ=255)

                            gCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCa                                                             >  1:3659906/1‑93 (MQ=255)

                            gCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCa                                                             <  1:1623875/93‑1 (MQ=255)

                              gAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAAc                                                           >  2:408389/1‑93 (MQ=255)

                              gAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAAc                                                           <  2:2120657/93‑1 (MQ=255)

                               aaaTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAAcc                                                          <  1:2608133/93‑1 (MQ=255)

                                aaTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACca                                                         <  2:7288684/93‑1 (MQ=255)

                                aaTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACca                                                         <  2:5960817/93‑1 (MQ=255)

                                 aTGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACcac                                                        <  2:1371156/93‑1 (MQ=255)

                                  tGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACcaca                                                       <  2:5329580/93‑1 (MQ=255)

                                   ggCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAg                                                      <  1:6135965/93‑1 (MQ=255)

                                   ggCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAg                                                      >  1:1984190/1‑93 (MQ=255)

                                    gCGTTATCCATTGGAATCGAAAATTTGACTGTGTTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgc                                                     <  1:418858/93‑1 (MQ=255)

                                    gCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgc                                                     <  1:3391010/93‑1 (MQ=255)

                                     cGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgcg                                                    <  2:6237838/93‑1 (MQ=255)

                                      gTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAgcgc                                                   >  2:5627660/1‑93 (MQ=255)

                                       ttATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCa                                                  <  1:1216457/93‑1 (MQ=255)

                                       ttATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCa                                                  >  2:2519064/1‑93 (MQ=255)

                                          tCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCg                                               <  2:3282089/93‑1 (MQ=255)

                                          tCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCg                                               <  1:7546475/93‑1 (MQ=255)

                                           ccATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCgg                                              >  2:356475/1‑93 (MQ=255)

                                           ccATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCgg                                              >  1:4445010/1‑93 (MQ=255)

                                             aTTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTc                                            >  2:3442915/1‑93 (MQ=255)

                                             aTTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTc                                            <  2:4776350/93‑1 (MQ=255)

                                               tGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCat                                          <  1:1849210/93‑1 (MQ=255)

                                                ggAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCata                                         >  1:6852763/1‑93 (MQ=255)

                                                  aaTCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATc                                       >  1:5552542/1‑93 (MQ=255)

                                                    tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATccc                                     <  1:3799953/93‑1 (MQ=255)

                                                    tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATccc                                     >  1:7833687/1‑93 (MQ=255)

                                                    tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATccc                                     >  1:3278809/1‑93 (MQ=255)

                                                    tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATccc                                     >  2:4488628/1‑93 (MQ=255)

                                                    tCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATccc                                     >  1:470702/1‑93 (MQ=255)

                                                     cGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCg                                    >  1:4801814/1‑93 (MQ=255)

                                                     cGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCg                                    <  2:2447450/93‑1 (MQ=255)

                                                       aaaaTTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGcc                                  <  2:3261173/93‑1 (MQ=255)

                                                       aaaaTTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGcc                                  >  2:145573/1‑93 (MQ=255)

                                                        aaaTTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCa                                 <  2:4049631/93‑1 (MQ=255)

                                                         aaTTTGACTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAg                                >  1:5269192/1‑93 (MQ=255)

                                                          aTTTGACTGTGTTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGc                               <  1:6709291/93‑1 (MQ=255)

                                                               aCTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatca                          >  2:3918400/1‑93 (MQ=255)

                                                               aCTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatca                          <  1:5816132/93‑1 (MQ=255)

                                                                cTGTGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcat                         <  2:2257132/93‑1 (MQ=255)

                                                                cTGGGGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcat                         <  2:6527349/93‑1 (MQ=255)

                                                                 tgtgGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcatc                        >  2:690066/1‑93 (MQ=255)

                                                                 tgtgGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTatcatc                        >  2:7577617/1‑93 (MQ=255)

                                                                 tgtgGTCGTATCCCTAAACACCACACCGCCCGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCTTATCCCGCCAGCTatcaac                        <  1:6994377/93‑3 (MQ=255)

                                                                  gtgGTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCg                       <  2:1509303/93‑1 (MQ=255)

                                                                     gTCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAc                    >  1:413617/1‑93 (MQ=255)

                                                                      tCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAcc                   <  1:3938478/93‑1 (MQ=255)

                                                                      tCTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAcc                   >  1:2684671/1‑93 (MQ=255)

                                                                       cTTATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCAccc                  <  1:1526783/93‑1 (MQ=255)

                                                                         tATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCgg                >  1:7654111/1‑93 (MQ=255)

                                                                         tATCCATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCgg                >  2:630189/1‑93 (MQ=255)

                                                                          aTCCATCTGCCCCCCTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCCCCCGGc               <  1:1451675/93‑1 (MQ=255)

                                                                            ccATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCAt             <  2:7173261/93‑1 (MQ=255)

                                                                             cATCTGCCCCCCTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATc            <  2:6735009/93‑1 (MQ=255)

                                                                             cATCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATc            >  2:5267670/1‑93 (MQ=255)

                                                                              aTCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCg           >  2:7531489/1‑93 (MQ=255)

                                                                               tCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGt          >  2:5161171/1‑93 (MQ=255)

                                                                               tCTGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCACACCCGGCATCga          >  1:6890099/1‑92 (MQ=255)

                                                                                 tGCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAg        <  1:7507094/93‑1 (MQ=255)

                                                                                  tccccccTCCACCAGAAATATCAGTCCGCGGTGTGGTCAACCACAGCGCATCCGTCATATCCCGCCAGCTATCATCGCCCCCGGCATCGTAgg       <  2:4616047/92‑1 (MQ=255)

                                                                                  gCCCCACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAgg       <  2:6648442/93‑1 (MQ=255)

                                                                                   ccccACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGt      >  1:112241/1‑93 (MQ=255)

                                                                                     ccACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGttt    <  1:3505203/93‑1 (MQ=255)

                                                                                     ccACTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGttt    <  1:204653/93‑1 (MQ=255)

                                                                                      cACTCCGCCAGAGATATCAGCCCACGGTGCGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGtttt   <  1:2838992/93‑1 (MQ=255)

                                                                                       aCTCCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGttttt  >  1:6322407/1‑93 (MQ=255)

                                                                                       aCTCCGCCAGAGATATCAGTCCACGGCGTGGTCAAACACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGttttt  >  2:7799180/1‑93 (MQ=255)

AACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTT  >  NC_000913/304459‑304638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`