BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,861,203	A→G	A282A (GCA→GCG)	b2737 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,861,203	0	A	G	99.0%	902.5 / ‑2.7	292	A282A (GCA→GCG)	b2737	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/3); new base G (145/144); total (145/147)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 306 total aligned reads displayed.

GTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGT  >  NC_000913/2861114‑2861292

gTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGcgcg                                                                                         >  2:2603683/1‑93 (MQ=255)

gTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGgcgcgc                                                                                        <  1:5064847/93‑1 (MQ=255)

gTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGgcgcgc                                                                                        <  1:4186130/93‑1 (MQ=255)

 tAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCt                                                                                       >  1:4662743/1‑93 (MQ=255)

 tAGTGCTCTCCGGTTCATGCTCTAAAATGACCATCCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCACGCt                                                                                       <  1:7615833/93‑1 (MQ=255)

   gTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGc                                                                                     >  2:6747883/1‑93 (MQ=255)

   gTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGc                                                                                     <  2:822307/93‑1 (MQ=255)

    tGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGcc                                                                                    >  1:292466/1‑93 (MQ=255)

     gCTCTCCGGTTCATGCTCTCAAATGACCAAACGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCct                                                                                   >  2:2041489/1‑93 (MQ=255)

      ctctCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCctc                                                                                  <  2:6673159/93‑1 (MQ=255)

      ctctCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCctc                                                                                  <  1:3264397/93‑1 (MQ=255)

         tcCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCa                                                                               >  2:769891/1‑93 (MQ=255)

          ccGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCGCCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCaa                                                                              <  2:7155013/93‑1 (MQ=255)

           cGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCaa                                                                              >  2:6957232/1‑93 (MQ=255)

           cGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAAt                                                                             <  1:2213713/93‑1 (MQ=255)

            ggTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAAtt                                                                            <  1:5059338/93‑1 (MQ=255)

             gTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCATTTg                                                                           >  1:2698732/1‑93 (MQ=255)

              ttCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGa                                                                          >  1:4418924/1‑93 (MQ=255)

              ttCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGa                                                                          >  1:7495448/1‑93 (MQ=255)

              ttCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGa                                                                          >  1:7736201/1‑93 (MQ=255)

              ttCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGa                                                                          >  2:3964457/1‑93 (MQ=255)

               tCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGaa                                                                         >  1:6885159/1‑93 (MQ=255)

                cATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGaa                                                                         >  1:245664/1‑93 (MQ=255)

                cATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGaaa                                                                        <  2:1259655/93‑1 (MQ=255)

                 aTGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAAc                                                                       <  2:781504/93‑1 (MQ=255)

                  tGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAAct                                                                      <  1:1091796/93‑1 (MQ=255)

                  tGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAAct                                                                      <  1:6012890/93‑1 (MQ=255)

                    ctctCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAActct                                                                    >  1:6840000/1‑93 (MQ=255)

                    ctctCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAActct                                                                    <  1:3611124/93‑1 (MQ=255)

                      ctcAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTgg                                                                  >  2:1876780/1‑93 (MQ=255)

                       tcAAATGACCAACCGCCAGGTAGCACATTCCCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGc                                                                 <  2:1824733/93‑1 (MQ=255)

                       tcAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCCGCCTCTCAATTGAAACTCTGGc                                                                 >  2:6871003/1‑93 (MQ=255)

                        cAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGc                                                                 <  2:1473306/93‑1 (MQ=255)

                        cAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGcc                                                                >  1:6585060/1‑93 (MQ=255)

                         aaaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGcc                                                                >  2:5889417/1‑93 (MQ=255)

                         aaaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCg                                                               >  1:2169214/1‑93 (MQ=255)

                          aaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGc                                                              >  2:4915913/1‑93 (MQ=255)

                          aaTGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGc                                                              >  2:5812230/1‑93 (MQ=255)

                            tGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCtt                                                            >  1:7375325/1‑93 (MQ=255)

                             gACCAGCCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTa                                                           <  2:1180830/93‑1 (MQ=255)

                             gACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTa                                                           <  2:5005441/93‑1 (MQ=255)

                              aCCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTAt                                                          <  2:1991662/93‑1 (MQ=255)

                                  aCCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGcac                                                      >  1:4285545/1‑93 (MQ=255)

                                    cGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCacac                                                    <  1:1138767/93‑1 (MQ=255)

                                      ccAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGa                                                  <  2:2816726/93‑1 (MQ=255)

                                       cAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGaa                                                 >  1:4723662/1‑93 (MQ=255)

                                        aGGTAGCACATTACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGaa                                                 >  2:2884771/1‑93 (MQ=255)

                                        aGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAAc                                                <  2:7401313/93‑1 (MQ=255)

                                        aGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAAc                                                >  2:6378392/1‑93 (MQ=255)

                                        aGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAAc                                                <  1:1566651/93‑1 (MQ=255)

                                        aGGTAGCACATTACCGTCAAATGGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAAc                                                <  1:1444645/93‑1 (MQ=255)

                                         ggTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACt                                               <  1:6182617/93‑1 (MQ=255)

                                          gTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTg                                              <  1:6679127/93‑1 (MQ=255)

                                          gTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTg                                              <  2:5402292/93‑1 (MQ=255)

                                           tAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTgg                                             >  1:6479207/1‑93 (MQ=255)

                                             gCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCa                                           >  2:4557926/1‑93 (MQ=255)

                                                 aTTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGt                                       <  2:3013831/93‑1 (MQ=255)

                                                 aTTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGt                                       <  1:3445957/93‑1 (MQ=255)

                                                 aTTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGt                                       <  2:2155510/93‑1 (MQ=255)

                                                   tACCGTCAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCGCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTg                                      <  1:6771513/93‑1 (MQ=255)

                                                   tACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTgg                                     <  2:2713826/93‑1 (MQ=255)

                                                   tACCGTCAAATGGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTgg                                     <  1:1686461/93‑1 (MQ=255)

                                                    aCCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTggg                                    <  1:3754483/93‑1 (MQ=255)

                                                    aCCGTCAAACTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTgg                                     <  1:3929909/93‑1 (MQ=255)

                                                      cGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGtt                                  >  1:1257765/1‑93 (MQ=255)

                                                       gTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTc                                 >  1:1814644/1‑93 (MQ=255)

                                                       gTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTc                                 <  2:4635784/93‑1 (MQ=255)

                                                        tCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCt                                <  1:2303418/93‑1 (MQ=255)

                                                         cAAATTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCt                                <  2:4235867/93‑1 (MQ=255)

                                                         cAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTg                               <  2:549545/93‑1 (MQ=255)

                                                         aaaaTTGCTCCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTg                               <  2:7630489/92‑1 (MQ=255)

                                                          aaaTTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTgg                              >  2:6519285/1‑93 (MQ=255)

                                                           aaTTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTggg                             <  1:5819730/93‑1 (MQ=255)

                                                            aTTGCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTggg                             >  2:3575745/1‑93 (MQ=255)

                                                            aTTGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGc                            <  1:6928266/93‑1 (MQ=255)

                                                             ttGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAATCTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGcc                           <  1:4366106/93‑1 (MQ=255)

                                                             ttGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGcc                           >  1:257077/1‑93 (MQ=255)

                                                             ttGCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGcc                           >  2:339067/1‑93 (MQ=255)

                                                               gCACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCa                          <  2:4443852/93‑1 (MQ=255)

                                                               gCACCAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAg                         >  1:490490/1‑93 (MQ=255)

                                                                cACCAGCCCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAg                         <  1:1146193/93‑1 (MQ=255)

                                                                   cAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGaaa                     >  2:5120769/1‑93 (MQ=255)

                                                                   cAG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGaaa                     <  1:4518198/93‑1 (MQ=255)

                                                                    aG‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAg                    >  1:5455364/1‑93 (MQ=255)

                                                                     g‑CCCGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAgt                   >  2:944895/1‑93 (MQ=255)

                                                                        ccGTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAgtgt                 >  2:4125998/1‑93 (MQ=255)

                                                                          gTGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTAc               <  2:6499929/93‑1 (MQ=255)

                                                                           tGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACt              <  2:5830095/93‑1 (MQ=255)

                                                                           tGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACt              <  2:5240402/93‑1 (MQ=255)

                                                                           tGAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACACTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACt              <  2:7019040/93‑1 (MQ=255)

                                                                            gAAGTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACtt             <  2:2298916/93‑1 (MQ=255)

                                                                               gTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCt          >  2:1014095/1‑93 (MQ=255)

                                                                               gTTGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCt          >  1:5732464/1‑93 (MQ=255)

                                                                                ttGATGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTc         <  2:4352542/93‑1 (MQ=255)

                                                                                   aTGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCAc      <  1:6256799/93‑1 (MQ=255)

                                                                                   aTGTGGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCAc      <  1:4055900/93‑1 (MQ=255)

                                                                                    tgtgGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACt     >  1:5308488/1‑93 (MQ=255)

                                                                                     gtgGCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGGGTACTTGCTCCACTg    >  2:6462873/1‑93 (MQ=255)

                                                                                      gggCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTgg   <  2:1564351/92‑1 (MQ=255)

                                                                                       ggCGCGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGt  <  1:5151822/93‑1 (MQ=255)

GTAGTGCTCTCCGGTTCATGCTCTCAAATGACCAACCGCCAGGTAGCACATTACCGTCAAATTGCACCAG‑CCCGTGAAGTTGATGTGGCACGCTGCCTCTCAATTGAAACTCTGGCCGCTTATGCACACGAACTGGCAGAGTGGGTTCTGGGCCAGGAAAGTGTACTTGCTCCACTGGT  >  NC_000913/2861114‑2861292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`