BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	306,666	C→T	V631I (GTC→ATC)	yagX ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	306,666	0	C	T	99.3%	1028.5 / NA	287	V631I (GTC→ATC)	yagX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (140/145); minor base G (0/2); total (140/147)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

GCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGGAGATGG  >  NC_000913/306576‑306755

gcccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGt                                                                                         >  1:79386/1‑93 (MQ=255)

 cccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGtt                                                                                        >  2:5122677/1‑93 (MQ=255)

  ccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCGATTGATGTTc                                                                                       >  2:1478460/1‑93 (MQ=255)

  ccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCACGCTATTGATGTTc                                                                                       >  1:104387/1‑93 (MQ=255)

    gcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAg                                                                                     <  1:4310412/93‑1 (MQ=255)

    gcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAg                                                                                     >  1:7769366/1‑93 (MQ=255)

    gcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAg                                                                                     >  2:5833098/1‑93 (MQ=255)

    gcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAg                                                                                     >  2:4602247/1‑93 (MQ=255)

     cTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGc                                                                                    >  1:3258961/1‑93 (MQ=255)

       ggCAGCAGTGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGt                                                                                  <  1:4978358/93‑1 (MQ=255)

       ggCAGCAATGTTTTTACCCTGCCAGGCGGCGCTGCCATTTACGGCCAAGTTGGTATTGACTGAGCCGGCCGCGGCGCTATTAATGTTAACCGt                                                                                  >  2:3149847/1‑93 (MQ=255)

       ggCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGt                                                                                  >  1:2735394/1‑93 (MQ=255)

       ggCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGt                                                                                  <  1:7146191/93‑1 (MQ=255)

        gcagcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGtt                                                                                 >  2:2389193/1‑93 (MQ=255)

          agcaTTGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTcc                                                                               <  2:2957153/93‑1 (MQ=255)

          agcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTcc                                                                               <  2:1290439/93‑1 (MQ=255)

           gcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCg                                                                              >  2:1682355/1‑93 (MQ=255)

           gcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCg                                                                              >  2:6883006/1‑93 (MQ=255)

            caATTTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGc                                                                             <  2:7557884/93‑1 (MQ=255)

            caATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGc                                                                             <  1:6336478/93‑1 (MQ=255)

             aaTGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCt                                                                            >  1:7184149/1‑93 (MQ=255)

              aTGTTTTTCCCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTg                                                                           <  2:30604/93‑1 (MQ=255)

              aTGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTg                                                                           >  2:7907807/1‑93 (MQ=255)

               tGTTTTTACCCTGCCAGCCGCCGCTGCCATTGGCGGGCAATTTGGGTTTGACTTACCCGTCCGCCGCGCTATTGATGTTTAGCTTTCCGCTgg                                                                          >  2:5353605/1‑93 (MQ=255)

                 tttttACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCg                                                                        >  1:3990070/1‑93 (MQ=255)

                      aCCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACg                                                                   <  1:5236806/93‑1 (MQ=255)

                       cccTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGa                                                                  <  1:6925430/93‑1 (MQ=255)

                        ccTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAg                                                                 >  2:5501410/1‑93 (MQ=255)

                        ccTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCCCTGGCGTAACGAg                                                                 >  1:2237964/1‑93 (MQ=255)

                         cTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCACTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGc                                                                >  1:1027707/1‑93 (MQ=255)

                           gCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGt                                                              >  1:3033951/1‑93 (MQ=255)

                            ccAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTc                                                             >  2:1100706/1‑93 (MQ=255)

                              aGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGa                                                           <  2:7740159/93‑1 (MQ=255)

                               gCCGACGCTGCCATTGGCGGGCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGat                                                          >  1:1843038/1‑92 (MQ=255)

                                ccGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAAt                                                         <  1:4546621/93‑1 (MQ=255)

                                ccGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAAt                                                         <  1:6449407/93‑1 (MQ=255)

                                 cGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAAtt                                                        <  2:4352180/93‑1 (MQ=255)

                                 cGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAAtt                                                        >  2:640419/1‑93 (MQ=255)

                                   aCGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATtgt                                                      <  2:1830338/93‑1 (MQ=255)

                                       tGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCAt                                                  >  2:6236511/1‑93 (MQ=255)

                                       tGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCAt                                                  <  1:2963196/93‑1 (MQ=255)

                                        gCCATTGGCGGTCAAGTTGGTATTGGCGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATa                                                 <  1:6641639/93‑1 (MQ=255)

                                        gCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATa                                                 <  2:6506168/93‑1 (MQ=255)

                                        gCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATa                                                 <  1:6102844/93‑1 (MQ=255)

                                        gCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATa                                                 >  2:3120397/1‑93 (MQ=255)

                                         ccATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATAc                                                >  2:3678794/1‑93 (MQ=255)

                                          cATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACg                                               >  1:4864/1‑93 (MQ=255)

                                          cATTGGCGGTCAAGTTGGCATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACg                                               >  2:6085589/1‑93 (MQ=255)

                                           aTTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGc                                              <  2:2087361/93‑1 (MQ=255)

                                              ggCGTTCAAGTTGGTATTGACGTAGCTGCCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGcccc                                           <  2:27302/93‑1 (MQ=255)

                                              ggCGGTCAAGTTGGTTTTGTAGTAGCCGTCCGCCGCGCTATTGATGTTAAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGcccc                                           <  1:7041828/93‑1 (MQ=255)

                                              ggCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGcccc                                           <  1:348661/93‑1 (MQ=255)

                                                  gTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCccgccg                                       <  1:2390653/93‑1 (MQ=255)

                                                  gTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCccgccg                                       >  2:6924341/1‑93 (MQ=255)

                                                   tCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCcgccgc                                      >  1:4450345/1‑93 (MQ=255)

                                                    cAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCt                                     >  1:2008915/1‑93 (MQ=255)

                                                    cAAGTTGGTATTGACGTAGCCATCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCt                                     >  2:5016132/1‑93 (MQ=255)

                                                      aGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGa                                   >  2:3770995/1‑93 (MQ=255)

                                                       gTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAg                                  <  2:1731462/93‑1 (MQ=255)

                                                        ttGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAgg                                 >  1:6789093/1‑93 (MQ=255)

                                                        ttGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAgg                                 <  1:5549680/93‑1 (MQ=255)

                                                        ttGGTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAgg                                 >  1:984920/1‑93 (MQ=255)

                                                           gTATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGtt                              >  1:3927477/1‑93 (MQ=255)

                                                            tATTGACGTAGCCGTCCGCCGCTCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGttt                             >  1:1399944/1‑93 (MQ=255)

                                                            tATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGttt                             >  1:5321367/1‑93 (MQ=255)

                                                            tATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGttt                             <  1:5187464/93‑1 (MQ=255)

                                                            tATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGttt                             >  2:7008850/1‑93 (MQ=255)

                                                            tATTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGttt                             <  2:349786/93‑1 (MQ=255)

                                                             aTTGACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGtttt                            <  2:3362134/93‑1 (MQ=255)

                                                                gACGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtc                         <  2:2030149/93‑1 (MQ=255)

                                                                 aCGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtca                        <  1:897699/93‑1 (MQ=255)

                                                                 aCGTAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtca                        <  1:3522391/93‑1 (MQ=255)

                                                                  cttAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtcat                       <  1:1627276/91‑1 (MQ=255)

                                                                    tAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtcatcc                     >  2:6952082/1‑92 (MQ=255)

                                                                    tAGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGtcatca                     >  2:3567049/1‑93 (MQ=255)

                                                                     aGCCGTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCAc                    <  2:2521309/93‑1 (MQ=255)

                                                                         gTCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGt                <  2:6249976/93‑1 (MQ=255)

                                                                          tCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTa               <  1:4739033/93‑1 (MQ=255)

                                                                          tCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTa               <  1:5680198/93‑1 (MQ=255)

                                                                          tCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTa               <  1:5854775/93‑1 (MQ=255)

                                                                          tCCGCCGCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTa               <  1:6901591/93‑1 (MQ=255)

                                                                           ccgccgCGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTAt              >  1:3454911/1‑93 (MQ=255)

                                                                            cgccgcGCTATTGATGTTCAGCGTTCCGCTGACGTAACGAGCGTCGAATTGTGCACACGCCCCGCCGCTGAGGTTTTTGTCATCACCGGTATc             <  2:2332094/93‑1 (MQ=255)

                                                                              ccgcGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGc           <  2:4175064/93‑1 (MQ=255)

                                                                              ccgcGCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGc           <  2:6545805/93‑1 (MQ=255)

                                                                               cgcgCTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGcc          >  2:2452891/1‑93 (MQ=255)

                                                                                  gcTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGga       >  1:4808487/1‑93 (MQ=255)

                                                                                  gcTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGga       <  2:764323/93‑1 (MQ=255)

                                                                                  gcTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGga       >  1:7392504/1‑93 (MQ=255)

                                                                                   cTATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgag      >  1:2589818/1‑93 (MQ=255)

                                                                                    tATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgagg     >  2:2276281/1‑92 (MQ=255)

                                                                                    tATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgaga     <  1:7641228/93‑1 (MQ=255)

                                                                                    tATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgaga     >  1:6488029/1‑93 (MQ=255)

                                                                                    tATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgaga     >  2:5715167/1‑93 (MQ=255)

                                                                                    tATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgaga     >  2:7320519/1‑93 (MQ=255)

                                                                                    tATTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGgaga     >  2:2773863/1‑93 (MQ=255)

                                                                                     aTTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGGAGAt    <  1:3759230/93‑1 (MQ=255)

                                                                                     aTTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGGAGAt    <  1:1166079/93‑1 (MQ=255)

                                                                                     aTTGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGGAGAt    <  2:4118323/93‑1 (MQ=255)

                                                                                       tGATGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGGAGATgc  >  1:1963990/1‑92 (MQ=255)

GCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATTGACGTTCAGCGTTCCGCTGGCGTAACGAGCGTCGAATTGTGCATACGCCCCGCCGCTGAGGGTTTTGTCATCACCGGTATCGCCGGAGATGG  >  NC_000913/306576‑306755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`