BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,877,392	T→C	E135G (GAG→GGG)	b2755 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,877,392	0	T	C	99.7%	1021.0 / ‑5.7	322	E135G (GAG→GGG)	b2755	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (149/172); total (149/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 327 total aligned reads displayed.

AGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGC  >  NC_000913/2877303‑2877480

aGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTa                                                                                       >  1:1538653/1‑93 (MQ=255)

aGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTa                                                                                       <  1:358531/93‑1 (MQ=255)

aGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTa                                                                                       >  2:534822/1‑93 (MQ=255)

 gCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTAc                                                                                      <  2:2924517/93‑1 (MQ=255)

 gCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTAc                                                                                      >  1:6144306/1‑93 (MQ=255)

   gACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACgg                                                                                    >  1:7201323/1‑93 (MQ=255)

   gACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACgg                                                                                    >  1:3032700/1‑93 (MQ=255)

   gACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACgg                                                                                    >  2:1281328/1‑93 (MQ=255)

      gTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAgc                                                                                 <  2:3995032/93‑1 (MQ=255)

       tccattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCCTCTATACCTCTGAGTTGCCCTACGGAgcg                                                                                <  2:5664867/93‑1 (MQ=255)

         cattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGcc                                                                              >  2:4133208/1‑93 (MQ=255)

         cattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGcc                                                                              >  2:4811439/1‑93 (MQ=255)

          attccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCg                                                                             <  2:3293860/93‑1 (MQ=255)

           ttccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCgg                                                                            <  1:1977651/93‑1 (MQ=255)

             ccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGc                                                                          >  1:5576275/1‑93 (MQ=255)

              catGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGTGTTGCCCTACGGAGCGCCGGGCa                                                                         >  2:5374505/1‑93 (MQ=255)

              catGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCa                                                                         >  1:1192893/1‑93 (MQ=255)

               atGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAg                                                                        <  1:2887298/93‑1 (MQ=255)

               atGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAg                                                                        >  1:384182/1‑93 (MQ=255)

               atGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAg                                                                        <  1:6507472/93‑1 (MQ=255)

                   cACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGgcgc                                                                    <  1:3974806/93‑1 (MQ=255)

                   cACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGgcgc                                                                    <  1:7507951/93‑1 (MQ=255)

                    aCGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCa                                                                   <  2:4351608/93‑1 (MQ=255)

                    aCGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCa                                                                   >  1:1648153/1‑93 (MQ=255)

                     cGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAg                                                                  >  1:4322938/1‑93 (MQ=255)

                      gCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAgg                                                                 >  1:7001491/1‑93 (MQ=255)

                      gCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAgg                                                                 >  1:6377194/1‑93 (MQ=255)

                      gCCGTATTGCTTCGCCAGAAGTGCGTAGGCTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAgg                                                                 >  2:4205464/1‑93 (MQ=255)

                        cGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGtt                                                               <  2:7858892/93‑1 (MQ=255)

                        cGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGtt                                                               >  2:5907211/1‑93 (MQ=255)

                        cGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGtt                                                               <  2:2707670/93‑1 (MQ=255)

                         gTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtc                                                              >  2:4995207/1‑93 (MQ=255)

                         gTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtc                                                              >  1:2683163/1‑93 (MQ=255)

                          tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGttgt                                                             >  2:5989881/1‑91 (MQ=255)

                          tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                             >  2:4479067/1‑93 (MQ=255)

                          tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                             >  2:5121826/1‑93 (MQ=255)

                           aTTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtctc                                                            <  2:4640427/93‑1 (MQ=255)

                            ttGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTcc                                                           >  2:1886039/1‑93 (MQ=255)

                             tGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCa                                                          <  2:1515218/93‑1 (MQ=255)

                               cTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCaaa                                                        <  1:3273645/93‑1 (MQ=255)

                                ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                       >  1:5721858/1‑93 (MQ=255)

                                ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                       <  2:6370065/93‑1 (MQ=255)

                                ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                       <  1:6697754/93‑1 (MQ=255)

                                 tCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAcc                                                      >  2:5509611/1‑93 (MQ=255)

                                 tCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAcc                                                      <  2:3694346/93‑1 (MQ=255)

                                   gCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGa                                                    <  1:6266272/93‑1 (MQ=255)

                                   gCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGa                                                    <  2:5214775/93‑1 (MQ=255)

                                   gCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGa                                                    >  2:2137978/1‑93 (MQ=255)

                                    ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  2:7181637/93‑1 (MQ=255)

                                    ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  1:5621608/93‑1 (MQ=255)

                                    ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                   <  1:3125177/93‑1 (MQ=255)

                                     cAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                  >  1:6645445/1‑93 (MQ=255)

                                     cAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                  <  2:3191194/93‑1 (MQ=255)

                                       gAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGtt                                                >  1:4745893/1‑93 (MQ=255)

                                        aaGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTc                                               <  1:6882972/93‑1 (MQ=255)

                                           tGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCaaa                                            >  1:1756856/1‑93 (MQ=255)

                                            gCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAAc                                           <  1:5890630/93‑1 (MQ=255)

                                             cGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACa                                          <  2:693738/93‑1 (MQ=255)

                                              gTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAt                                         >  2:2429632/1‑93 (MQ=255)

                                               tAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtt                                        >  2:117386/1‑93 (MQ=255)

                                               tAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtt                                        <  2:2405656/93‑1 (MQ=255)

                                                aGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAttt                                       <  2:164424/93‑1 (MQ=255)

                                                aGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAttt                                       <  1:336913/93‑1 (MQ=255)

                                                aGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAttt                                       >  2:802963/1‑93 (MQ=255)

                                                 ggTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtttt                                      <  2:855225/93‑1 (MQ=255)

                                                  gTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTta                                     <  1:4982373/93‑1 (MQ=255)

                                                  gTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTta                                     >  2:4709555/1‑93 (MQ=255)

                                                   ttGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtac                                    <  1:6411435/93‑1 (MQ=255)

                                                      cccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgta                                 <  2:7356161/93‑1 (MQ=255)

                                                      cccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgta                                 >  2:1433505/1‑93 (MQ=255)

                                                       ccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCGTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCACACATTTtacgtac                                >  1:733436/1‑93 (MQ=255)

                                                       ccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgtac                                <  2:3526786/93‑1 (MQ=255)

                                                        cGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgtacg                               <  2:6257763/93‑1 (MQ=255)

                                                         gCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGa                              >  1:6042087/1‑93 (MQ=255)

                                                         gCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGa                              >  1:5010240/1‑93 (MQ=255)

                                                         gCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGa                              >  2:4927493/1‑93 (MQ=255)

                                                          cACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGAc                             <  1:44898/93‑1 (MQ=255)

                                                            cgcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCt                           >  2:6606667/1‑93 (MQ=255)

                                                                aCTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAg                       >  1:2133168/1‑93 (MQ=255)

                                                                 cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                      <  1:605716/93‑1 (MQ=255)

                                                                 cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                      <  2:1408318/93‑1 (MQ=255)

                                                                 cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                      >  1:1374693/1‑93 (MQ=255)

                                                                 cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                      <  1:5321366/93‑1 (MQ=255)

                                                                 cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                      <  1:3435438/93‑1 (MQ=255)

                                                                  tGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGAc                     <  1:1827783/93‑1 (MQ=255)

                                                                  tGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGAc                     >  1:7933547/1‑93 (MQ=255)

                                                                   gCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGGTCAAACATTTTACGTACGACCTTCCGACg                    >  1:251660/1‑93 (MQ=255)

                                                                      ttCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCaa                 >  1:4630485/1‑93 (MQ=255)

                                                                      ttCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCaa                 <  1:106813/93‑1 (MQ=255)

                                                                         tataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATc              >  2:7302567/1‑93 (MQ=255)

                                                                          ataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCt             <  2:2768839/93‑1 (MQ=255)

                                                                           ttcttctGTGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCTAAACCGAAGTTCAAACATTTTATGTACGACCATCAGACGCAAATCtt            >  2:7046591/5‑93 (MQ=255)

                                                                            aCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTc           <  2:7530217/93‑1 (MQ=255)

                                                                             ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa          <  1:7688104/93‑1 (MQ=255)

                                                                             ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa          <  1:2380645/93‑1 (MQ=255)

                                                                             ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa          <  2:3847715/93‑1 (MQ=255)

                                                                               tctGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATc        >  2:6727547/1‑93 (MQ=255)

                                                                               tctGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATc        >  1:2473713/1‑93 (MQ=255)

                                                                                cagagaTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATcc       <  1:2832592/91‑1 (MQ=255)

                                                                                     ttGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGc  <  2:7426357/93‑1 (MQ=255)

AGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGC  >  NC_000913/2877303‑2877480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`