BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,915,759	A→G	D894G (GAT→GGT)	barA →	sensor‑regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,915,759	0	A	G	99.6%	860.4 / ‑5.2	274	D894G (GAT→GGT)	barA	sensor‑regulator
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (146/127); total (146/128)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 306 total aligned reads displayed.

ATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTC  >  NC_000913/2915669‑2915848

aTGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGtt                                                                                         <  2:4718866/93‑1 (MQ=255)

aTGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGtt                                                                                         <  2:6440935/93‑1 (MQ=255)

aTGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGtt                                                                                         <  2:5828461/93‑1 (MQ=255)

aTGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGtt                                                                                         >  2:6106946/1‑93 (MQ=255)

aTGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGtt                                                                                         <  2:4010718/93‑1 (MQ=255)

 tGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGttt                                                                                        <  2:4531405/93‑1 (MQ=255)

 tGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGttg                                                                                        >  1:2683433/1‑92 (MQ=255)

  ggCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTg                                                                                       <  2:1595266/93‑1 (MQ=255)

      gTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAAc                                                                                   >  1:5622051/1‑93 (MQ=255)

        tGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCg                                                                                 <  2:7049293/93‑1 (MQ=255)

           ggCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAg                                                                              >  1:6261236/1‑93 (MQ=255)

            gCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgc                                                                             <  1:6887635/93‑1 (MQ=255)

            gCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgc                                                                             >  2:2937560/1‑93 (MQ=255)

            gCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgc                                                                             <  2:3494850/93‑1 (MQ=255)

             cTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgct                                                                            <  2:2707510/93‑1 (MQ=255)

              tataGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgctg                                                                           >  1:116603/1‑93 (MQ=255)

               ataGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgctgc                                                                          >  1:2312276/1‑93 (MQ=255)

               ataGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgctgc                                                                          >  2:4084830/1‑93 (MQ=255)

                taGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAgctgct                                                                         >  1:4199549/1‑93 (MQ=255)

                 aGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGctgctg                                                                        <  2:3187048/93‑1 (MQ=255)

                     gtgtGCCACGTATGAAGAATCTCTGCCAACTGATCGAAAAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAAc                                                                    >  2:4856168/1‑93 (MQ=255)

                        tgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGt                                                                 <  1:5089284/93‑1 (MQ=255)

                         gCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGtt                                                                <  2:5990360/93‑1 (MQ=255)

                         gCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGtt                                                                <  1:4760626/93‑1 (MQ=255)

                          ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTg                                                               <  2:659201/93‑1 (MQ=255)

                          ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTg                                                               <  2:3346890/93‑1 (MQ=255)

                           cACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTgg                                                              >  2:7588501/1‑93 (MQ=255)

                           cACCTATGAAGAATCTCTGCCAACGGATCGAACAACAGCTCCGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTgg                                                              <  1:1072253/93‑1 (MQ=255)

                            aCGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                             >  2:2822054/1‑93 (MQ=255)

                            aCGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGa                                                             <  1:3342498/93‑1 (MQ=255)

                               tATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACga                                                          >  2:3527474/1‑93 (MQ=255)

                                aTGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACgag                                                         >  1:5352611/1‑93 (MQ=255)

                                 tGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACgaga                                                        >  1:672325/1‑93 (MQ=255)

                                  gaagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGAt                                                       <  2:2375434/93‑1 (MQ=255)

                                  gaagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGAt                                                       >  2:5340512/1‑93 (MQ=255)

                                  gaagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGAt                                                       >  2:1169149/1‑93 (MQ=255)

                                    agaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATgg                                                     >  2:4387643/1‑93 (MQ=255)

                                     gaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGa                                                    >  1:5677076/1‑93 (MQ=255)

                                      aaTCTGTGCTACCAGATCGAACACCAGCTCCGTAGCGGTACTAAGGAAGAAGGTTTGGATCCGGAGCTGCTTGAACTGTTGGACGAGATGGAt                                                   <  2:6977632/93‑1 (MQ=255)

                                       aTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGGTTGGAACCGGAGCTGCTGGAACTGTTGGCCGAGATGGATa                                                  >  2:1512796/1‑93 (MQ=255)

                                        tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATaa                                                 <  2:3762320/93‑1 (MQ=255)

                                        tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATaa                                                 >  1:3253141/1‑93 (MQ=255)

                                        tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATaa                                                 <  2:2437136/93‑1 (MQ=255)

                                         ctctGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAAt                                                <  1:607147/93‑1 (MQ=255)

                                         ctctGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAAt                                                <  1:6179292/93‑1 (MQ=255)

                                          tctGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATg                                               <  2:5436139/93‑1 (MQ=255)

                                           ctGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGt                                              <  1:341685/93‑1 (MQ=255)

                                            tGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTc                                             >  2:507328/1‑93 (MQ=255)

                                            tGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTc                                             <  2:5888288/93‑1 (MQ=255)

                                                   tGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcgcg                                      >  1:7149949/1‑93 (MQ=255)

                                                   tGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcgcg                                      >  1:7914367/1‑93 (MQ=255)

                                                    gATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGa                                     >  2:7817658/1‑93 (MQ=255)

                                                    gATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGa                                     >  2:7499754/1‑93 (MQ=255)

                                                     aTCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGaa                                    <  2:2208781/93‑1 (MQ=255)

                                                     aTCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTCTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGaa                                    <  2:1003209/93‑1 (MQ=255)

                                                      tCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAg                                   >  1:5884588/1‑93 (MQ=255)

                                                      tCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAg                                   <  1:7760717/93‑1 (MQ=255)

                                                        gAACAACAGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGcc                                 >  2:6118780/1‑93 (MQ=255)

                                                          acaacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAg                               >  1:5779340/1‑93 (MQ=255)

                                                           caacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGc                              >  2:7446404/1‑93 (MQ=255)

                                                           caacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGc                              >  1:5223640/1‑93 (MQ=255)

                                                            aacaGCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCa                             >  1:1519060/1‑93 (MQ=255)

                                                                gCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAAt                         >  2:6192098/1‑93 (MQ=255)

                                                                gCTACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAAt                         >  1:199916/1‑93 (MQ=255)

                                                                  tACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtc                       <  1:2910941/93‑1 (MQ=255)

                                                                  tACGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtc                       >  2:6747139/1‑93 (MQ=255)

                                                                   aCGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtct                      >  1:3614363/1‑93 (MQ=255)

                                                                   aCGTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATtct                      <  1:6658060/93‑1 (MQ=255)

                                                                     gTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCg                    >  1:2556912/1‑93 (MQ=255)

                                                                     gTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCg                    >  1:3762249/1‑93 (MQ=255)

                                                                     gTAGCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCg                    >  1:5203266/1‑93 (MQ=255)

                                                                        gCGGTACTAAGGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGt                 >  1:3908636/1‑93 (MQ=255)

                                                                        gCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCggga                 >  2:7687630/1‑92 (MQ=255)

                                                                        gCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGt                 <  1:1490990/93‑1 (MQ=255)

                                                                        gCGGTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGt                 <  2:1304516/93‑1 (MQ=255)

                                                                          ggTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTaa               <  2:5184229/93‑1 (MQ=255)

                                                                          ggTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTaa               >  1:2999221/1‑93 (MQ=255)

                                                                          ggTACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTaa               <  2:4244917/93‑1 (MQ=255)

                                                                            tACTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATg             <  1:86899/93‑1 (MQ=255)

                                                                             aCTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATgg            >  1:210446/1‑93 (MQ=255)

                                                                              cTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGa           <  2:5573048/93‑1 (MQ=255)

                                                                              cTAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGa           <  2:2499218/93‑1 (MQ=255)

                                                                               tAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTTGGGTAATGGAt          >  1:7477935/1‑93 (MQ=255)

                                                                               tAAAGAAGAAGGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGAt          >  2:7888009/1‑93 (MQ=255)

                                                                                 aagaagaagGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTCATGGATGt        >  2:2000924/1‑93 (MQ=255)

                                                                                 aagaagaagGTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGt        <  1:1017267/93‑1 (MQ=255)

                                                                                  agaagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTc       <  1:4553299/93‑1 (MQ=255)

                                                                                   gaagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCt      >  1:7294272/1‑93 (MQ=255)

                                                                                    aagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCtt     >  2:3106435/1‑93 (MQ=255)

                                                                                    aagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCtt     <  1:6517995/93‑1 (MQ=255)

                                                                                    aagaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCtt     >  1:3149282/1‑93 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    >  2:1561253/1‑93 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    >  2:5759545/1‑93 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    >  2:6937393/1‑93 (MQ=255)

                                                                                     agaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTg    <  1:4364698/93‑1 (MQ=255)

                                                                                     agaaggTTGGGAACCGGAGCTGCTAGAACTGTAGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCTAAATTCTCGGGTAATGGATGTCTTg    <  2:7254902/93‑1 (MQ=255)

                                                                                      gaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGt   >  1:5462717/1‑93 (MQ=255)

                                                                                      gaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGt   >  1:1698066/1‑93 (MQ=255)

                                                                                      gaaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGt   <  1:4888284/93‑1 (MQ=255)

                                                                                       aaggTTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTc  <  2:4433760/93‑1 (MQ=255)

ATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTC  >  NC_000913/2915669‑2915848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`