BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	307,684	T→C	L291L (TTA→TTG)	yagX ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	307,684	0	T	C	99.7%	897.5 / ‑5.4	288	L291L (TTA→TTG)	yagX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (164/123); total (165/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 288 total aligned reads displayed.

CGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCC  >  NC_000913/307595‑307773

cGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCaaa                                                                                        >  1:5660418/1‑93 (MQ=255)

  ggTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCaaaaa                                                                                      <  1:1164422/93‑1 (MQ=255)

  ggTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCaaaaa                                                                                      <  1:6233121/93‑1 (MQ=255)

    tATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAgg                                                                                    >  2:552227/1‑93 (MQ=255)

    tATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAgg                                                                                    >  1:1604072/1‑93 (MQ=255)

    tATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAgg                                                                                    >  2:806791/1‑93 (MQ=255)

     aTCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGc                                                                                   >  2:7794261/1‑93 (MQ=255)

     aTCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGc                                                                                   <  1:2606294/93‑1 (MQ=255)

     aTCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGc                                                                                   >  1:451820/1‑93 (MQ=255)

      tCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCg                                                                                  >  1:4920195/1‑93 (MQ=255)

        cACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGAt                                                                                >  1:365895/1‑93 (MQ=255)

         tcTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATc                                                                               <  1:44003/92‑1 (MQ=255)

         aCTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATc                                                                               >  1:5794481/1‑93 (MQ=255)

          cTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCa                                                                              <  1:2709424/93‑1 (MQ=255)

           ttCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCAc                                                                             >  2:6158032/1‑93 (MQ=255)

           ttCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCAc                                                                             >  2:6836977/1‑93 (MQ=255)

             cATGATTGCCCATAGGGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTg                                                                           <  2:2105730/93‑1 (MQ=255)

                gATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCg                                                                        >  2:6072421/1‑93 (MQ=255)

                 aTTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGt                                                                       >  2:5886804/1‑93 (MQ=255)

                    gCCCATAGTGAAGTTCTGAACGCTTAGTAGCCCCCCATCAGGGGTAAGAGGTACTCCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGc                                                                    <  1:3303367/93‑1 (MQ=255)

                       cATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGa                                                                 <  1:6887157/93‑1 (MQ=255)

                        aTAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGAc                                                                >  1:5154032/1‑93 (MQ=255)

                        aTAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGAc                                                                >  2:5993163/1‑93 (MQ=255)

                         tAGTGAAGTTCTGACCGCTTAGTAGCCGCCCATCACGGGGGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACt                                                               <  2:3627226/93‑1 (MQ=255)

                         tAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACt                                                               >  1:1339280/1‑93 (MQ=255)

                           gTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTgg                                                             >  2:6288253/1‑93 (MQ=255)

                            tGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgc                                                            >  2:1626921/1‑93 (MQ=255)

                            tGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgc                                                            <  1:948533/93‑1 (MQ=255)

                             gAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgct                                                           <  2:2619649/93‑1 (MQ=255)

                             gAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgct                                                           >  2:4594447/1‑93 (MQ=255)

                             gAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGGGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgct                                                           >  1:4885111/1‑93 (MQ=255)

                              aaGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgctg                                                          >  2:6394398/1‑93 (MQ=255)

                              aaGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgctg                                                          >  2:3798082/1‑93 (MQ=255)

                              aaGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgctg                                                          >  1:2347339/1‑93 (MQ=255)

                               aGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgctgc                                                         <  2:3396981/93‑1 (MQ=255)

                                gTTCTGACCGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGgctgct                                                        <  2:4064868/93‑1 (MQ=255)

                                 ttCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGctgctg                                                       >  2:5171225/1‑93 (MQ=255)

                                 ttCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGctgctg                                                       >  1:803330/1‑93 (MQ=255)

                                    tGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCg                                                    >  1:3992656/1‑93 (MQ=255)

                                    tGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCg                                                    <  1:4242859/93‑1 (MQ=255)

                                    tGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCg                                                    <  2:4536779/93‑1 (MQ=255)

                                    tGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGATGTCg                                                    >  2:7715076/1‑93 (MQ=255)

                                         ccTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAt                                               <  1:5526307/92‑1 (MQ=255)

                                         ccTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAt                                               <  1:7771311/92‑1 (MQ=255)

                                           ttAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAttg                                             >  2:6917519/1‑91 (MQ=255)

                                           ttAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtgg                                             >  1:6660094/1‑93 (MQ=255)

                                            tagtagCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtggg                                            >  2:5617767/1‑92 (MQ=255)

                                            tagtacCCCCCCATCACGGGTGAGATGTACTCCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGAtggt                                            <  1:428057/93‑1 (MQ=255)

                                               tagCCCCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGa                                         <  2:7177730/93‑1 (MQ=255)

                                                 gccgccCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGc                                       <  2:3250071/93‑1 (MQ=255)

                                                 gccgccCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGc                                       >  2:3043730/1‑93 (MQ=255)

                                                  ccgccCAGCCGGGGGGGGTTGTCCTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCt                                      <  1:1999407/93‑1 (MQ=255)

                                                   cgccCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTg                                     >  1:1379547/1‑93 (MQ=255)

                                                   cccccATCCCGGGGGAGATTTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTg                                     <  2:1484800/91‑1 (MQ=255)

                                                    gccCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTgg                                    >  2:4419055/1‑93 (MQ=255)

                                                    gccCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTgg                                    >  2:2518333/1‑93 (MQ=255)

                                                      ccATCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGcc                                  <  1:3474829/93‑1 (MQ=255)

                                                        accgcGGGTGAGATGTACTCCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTg                                <  1:1275961/89‑1 (MQ=255)

                                                        aTCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTg                                <  2:2283269/93‑1 (MQ=255)

                                                        aTCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTg                                >  2:1262706/1‑93 (MQ=255)

                                                         tCACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTgg                               >  2:7826794/1‑93 (MQ=255)

                                                          cACGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGt                              >  1:4068561/1‑93 (MQ=255)

                                                            cGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGGTGGCCTGGgtt                            >  2:7064451/1‑93 (MQ=255)

                                                            cGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGttt                            <  1:5068823/93‑1 (MQ=255)

                                                            cGGGTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGttt                            <  2:4563449/93‑1 (MQ=255)

                                                             gggTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGGGGAGCTGGCCTGGGTTc                           >  2:7500690/1‑93 (MQ=255)

                                                               gTGAGATGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTccc                         >  2:4779583/1‑93 (MQ=255)

                                                                 gagaTGTCCTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCa                       <  2:1691445/93‑1 (MQ=255)

                                                                 gagaTGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCa                       <  1:5900798/93‑1 (MQ=255)

                                                                 gagaTGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCa                       >  2:3534191/1‑93 (MQ=255)

                                                                 gagaTGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCa                       <  1:6394943/93‑1 (MQ=255)

                                                                  gaaTGTCCTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAg                      <  2:2888557/91‑1 (MQ=255)

                                                                  agaCGTTCTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAg                      <  1:7397067/93‑1 (MQ=255)

                                                                   gaTGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAgg                     >  1:7074045/1‑93 (MQ=255)

                                                                   gaTGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCGGCCGAAGAGGGTGGAGCTGGCCGGGGTTCCCCAgg                     >  1:2937818/1‑93 (MQ=255)

                                                                    aTGTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGa                    >  1:6521514/1‑93 (MQ=255)

                                                                     tGTACTTCTCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaa                   <  1:7106190/93‑1 (MQ=255)

                                                                      gtccTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  <  2:7280163/90‑1 (MQ=255)

                                                                      gTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  >  2:1820048/1‑93 (MQ=255)

                                                                      gTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  <  1:212588/93‑1 (MQ=255)

                                                                      gTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  <  2:1339279/93‑1 (MQ=255)

                                                                      gTACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  >  1:2456512/1‑93 (MQ=255)

                                                                      gTACTCCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  <  2:4331308/93‑1 (MQ=255)

                                                                      gTACTCCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGaaa                  <  2:3333010/93‑1 (MQ=255)

                                                                       tACTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAg                 >  2:6517174/1‑93 (MQ=255)

                                                                        cctccgcccgccGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAgg                <  1:3105436/89‑1 (MQ=255)

                                                                         cTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGc               >  2:2166059/1‑93 (MQ=255)

                                                                         cTTCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGc               >  1:475128/1‑93 (MQ=255)

                                                                          ttCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGcc              >  1:5390435/1‑93 (MQ=255)

                                                                          ttCGCCCGCCGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGcc              >  2:1344625/1‑93 (MQ=255)

                                                                              cccgccGCCGGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTaa          >  1:7274259/1‑93 (MQ=255)

                                                                               ccgccgccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTaaa         <  2:578738/93‑1 (MQ=255)

                                                                                 gccgccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATc       <  1:7532424/93‑1 (MQ=255)

                                                                                    gccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTc    <  1:3786209/93‑1 (MQ=255)

                                                                                    gccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTc    <  1:3189765/93‑1 (MQ=255)

                                                                                    gccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTc    <  2:2016250/93‑1 (MQ=255)

                                                                                    gccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTc    <  1:1764095/93‑1 (MQ=255)

                                                                                     ccgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTcc   <  2:7631124/93‑1 (MQ=255)

                                                                                      cgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTccc  >  1:7151056/1‑93 (MQ=255)

                                                                                      cgGCAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTccc  <  2:1969411/93‑1 (MQ=255)

CGGGTATCCACTTCATGATTGCCCATAGTGAAGTTCTGAACGCTTAGTAGCCGCCCATCACGGGTGAGATGTACTTCGCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCC  >  NC_000913/307595‑307773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`