BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,864	T→C	M169T (ATG→ACG)	b2810 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,864	0	T	C	99.0%	904.7 / ‑2.0	290	M169T (ATG→ACG)	b2810	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (149/138); total (151/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

Only 100 of 299 total aligned reads displayed.

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTC  >  NC_000913/2941774‑2941953

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                         >  2:4751467/1‑93 (MQ=255)

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                         <  1:4553296/93‑1 (MQ=255)

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                         >  1:1801233/1‑93 (MQ=255)

aaTTGCAGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                         <  1:2032657/93‑1 (MQ=255)

 aTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTc                                                                                        <  2:3541369/93‑1 (MQ=255)

  ttGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCg                                                                                       >  2:2337095/1‑93 (MQ=255)

   tGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGa                                                                                      <  1:3448863/93‑1 (MQ=255)

    gCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGaa                                                                                     <  2:5693407/93‑1 (MQ=255)

    gCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGaa                                                                                     <  2:531518/93‑1 (MQ=255)

     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAc                                                                                    <  2:223141/93‑1 (MQ=255)

      cGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACg                                                                                   >  1:6373921/1‑93 (MQ=255)

      cGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACg                                                                                   >  1:4742394/1‑93 (MQ=255)

       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                  >  1:1239671/1‑93 (MQ=255)

       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                  >  2:7868084/1‑93 (MQ=255)

         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                                <  2:1854730/93‑1 (MQ=255)

             tGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTgg                                                                            >  1:6482857/1‑93 (MQ=255)

              gcgcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGc                                                                           <  1:3846470/93‑1 (MQ=255)

                gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                         >  2:2725232/1‑93 (MQ=255)

                 cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                        >  1:3081951/1‑93 (MQ=255)

                 cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                        >  2:1326342/1‑93 (MQ=255)

                  aGCGACTGCCGGATGTCGTTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTTGCGTTGGGCCAGACGTCAAACGTTAACGGGGGtt                                                                       >  1:1955389/1‑93 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgggt                                                                       >  1:2123085/1‑91 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                       <  2:6925117/93‑1 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                       >  2:340579/1‑93 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                       <  1:5348347/93‑1 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGCCGAACGTTACTGGCGGtt                                                                       >  1:5813912/1‑93 (MQ=255)

                    cGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGc                                                                     >  1:3350882/1‑93 (MQ=255)

                     gACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGcc                                                                    >  2:6410476/1‑93 (MQ=255)

                      aCTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGccc                                                                   <  2:45065/93‑1 (MQ=255)

                      aCTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGccc                                                                   <  2:3855287/93‑1 (MQ=255)

                       cTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGcgcg                                                                  >  2:344722/1‑93 (MQ=255)

                       cTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCg                                                                  <  1:7392084/93‑1 (MQ=255)

                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                                >  2:1994955/1‑93 (MQ=255)

                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                                >  2:600299/1‑93 (MQ=255)

                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                                >  1:2616929/1‑93 (MQ=255)

                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                               >  1:3729356/1‑93 (MQ=255)

                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                               <  1:6914664/93‑1 (MQ=255)

                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                            <  2:1461600/93‑1 (MQ=255)

                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGCATCTg                                                            >  2:2388307/1‑93 (MQ=255)

                              aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                           >  1:4115787/1‑93 (MQ=255)

                               tGTCGATTTGTTGCCAGAACTGACTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgc                                                          <  2:4282594/93‑1 (MQ=255)

                                gTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTTGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAGCTgggg                                                         >  2:1793864/1‑91 (MQ=255)

                                   gATTTGTTGCCAGAACTTATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                      <  1:5277230/93‑1 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                      <  2:881415/93‑1 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                      <  1:6743101/93‑1 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                      <  1:2488703/93‑1 (MQ=255)

                                    aTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgag                                                     >  2:5427342/1‑93 (MQ=255)

                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagg                                                    >  2:6674966/1‑92 (MQ=255)

                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagc                                                    >  2:3991309/1‑93 (MQ=255)

                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTAGAACGTTACTGGCGGGTGCCCGGTTTCGGCgcgagc                                                    >  2:1714040/1‑93 (MQ=255)

                                      ttgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcg                                                   <  2:5864424/93‑1 (MQ=255)

                                       tgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcga                                                  <  1:6065824/93‑1 (MQ=255)

                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                                >  1:641022/1‑93 (MQ=255)

                                          tgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTa                                               <  2:5148626/93‑1 (MQ=255)

                                          tgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTa                                               <  2:3232953/93‑1 (MQ=255)

                                            ccAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAcc                                             <  2:917577/93‑1 (MQ=255)

                                             cAGAGCTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                            >  1:1630400/1‑93 (MQ=255)

                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                            <  1:1286645/93‑1 (MQ=255)

                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGTTCTGGCGGGAGCGATTACCtt                                           >  1:2600486/1‑93 (MQ=255)

                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                           >  2:6974982/1‑93 (MQ=255)

                                                  cTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGCGCGATTACCTTTGCt                                       >  1:2548765/1‑93 (MQ=255)

                                                    gATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGGGCGAGCGATTACCTTTGCTct                                     >  2:7904764/1‑92 (MQ=255)

                                                    gATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCa                                     >  1:2662880/1‑93 (MQ=255)

                                                     aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAt                                    <  1:4254876/93‑1 (MQ=255)

                                                      ttACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAtt                                   <  1:3988015/93‑1 (MQ=255)

                                                      ttACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAtt                                   <  1:2943749/93‑1 (MQ=255)

                                                        aCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                                 >  1:3584621/1‑93 (MQ=255)

                                                         cTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGCTCTGGCGCGAGCGATTACCTTTGCTCATTCac                                >  1:5047027/1‑92 (MQ=255)

                                                         cTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAg                                >  2:740422/1‑93 (MQ=255)

                                                          cccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGc                               <  1:1738206/92‑1 (MQ=255)

                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGcc                              >  2:7519992/1‑93 (MQ=255)

                                                            cccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCg                             >  2:7640873/1‑93 (MQ=255)

                                                              cGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCggg                           <  2:2810672/93‑1 (MQ=255)

                                                                tAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGGGCGATTACCTTTGCTCATTCAGCCGGGag                         >  1:5596646/1‑92 (MQ=255)

                                                                tAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAt                         >  1:7756123/1‑93 (MQ=255)

                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtg                        >  1:844174/1‑93 (MQ=255)

                                                                  gTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtgg                       <  1:7912145/93‑1 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggt                      <  2:4997865/93‑1 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                      >  1:7502551/1‑92 (MQ=255)

                                                                   gcGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggt                      <  1:3277489/92‑1 (MQ=255)

                                                                    cGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtg                     <  1:1040341/93‑1 (MQ=255)

                                                                     ggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                    >  1:6168579/1‑93 (MQ=255)

                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtggg                   >  1:1191381/1‑92 (MQ=255)

                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtga                 <  1:278412/93‑1 (MQ=255)

                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtga                 >  1:5926346/1‑93 (MQ=255)

                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCCGCCGGGGTGGTGGtgat                >  2:3729264/1‑93 (MQ=255)

                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat                <  2:2081313/93‑1 (MQ=255)

                                                                          cTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatg               <  1:7146009/93‑1 (MQ=255)

                                                                               gTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCCTTCAGCCGGGATGGTGGtgatggttgg          >  2:2891798/1‑92 (MQ=255)

                                                                               gTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgg          >  2:3075768/1‑92 (MQ=255)

                                                                               gTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttga          <  2:6520334/93‑1 (MQ=255)

                                                                               gTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGggatggttga          >  1:2253305/1‑93 (MQ=255)

                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat         <  2:6192742/93‑1 (MQ=255)

                                                                                 tGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatg        <  1:1486804/93‑1 (MQ=255)

                                                                                  gggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatgg       <  2:5609965/93‑1 (MQ=255)

                                                                                   ggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatggt      <  1:4182429/93‑1 (MQ=255)

                                                                                    gTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTg     >  1:7267164/1‑93 (MQ=255)

                                                                                       aGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTc  >  2:6547339/1‑93 (MQ=255)

                                                                                       aGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTc  <  1:5222505/93‑1 (MQ=255)

                                                                                       aGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTc  <  1:4955024/93‑1 (MQ=255)

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTC  >  NC_000913/2941774‑2941953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`