BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,112	T→C	intergenic (‑80/‑152)	b2817 ← / → argA	putative amidase/N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,112	0	T	C	100.0%	1235.0 / NA	386	intergenic (‑80/‑152)	b2817/argA	putative amidase/N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (168/218); total (168/218)

Only 100 of 388 total aligned reads displayed.

GATCCTGACATGCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCATAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTACACTTGC  >  NC_000913/2947022‑2947201

gATCCTGACATGCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACaa                                                                                         >  1:7391578/1‑93 (MQ=255)

  tCCTGACATGCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATc                                                                                       <  2:4656234/93‑1 (MQ=255)

  tCCTGACATGCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATc                                                                                       <  2:3441909/93‑1 (MQ=255)

  tCCTGACATGCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATc                                                                                       <  2:1328466/93‑1 (MQ=255)

           gCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCta                                                                              <  1:1138598/93‑1 (MQ=255)

           gCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCta                                                                              <  2:4262052/93‑1 (MQ=255)

            ccTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCTGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaa                                                                             >  2:7886987/1‑93 (MQ=255)

            ccTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaa                                                                             <  1:1664530/93‑1 (MQ=255)

            ccTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaa                                                                             <  1:2168819/93‑1 (MQ=255)

            ccTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaa                                                                             <  1:7491644/93‑1 (MQ=255)

            ccTCGCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaa                                                                             <  1:1045675/93‑1 (MQ=255)

             ctctCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaat                                                                            >  2:3043684/1‑93 (MQ=255)

             ctctCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaat                                                                            >  2:1735446/1‑93 (MQ=255)

              tctcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaata                                                                           <  2:7171503/93‑1 (MQ=255)

              tctcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaata                                                                           <  1:5836386/93‑1 (MQ=255)

               ctcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaataa                                                                          <  2:902708/93‑1 (MQ=255)

               ctcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCtaataa                                                                          <  1:2507787/93‑1 (MQ=255)

                tcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTCAACGACTAATTTGACCGGAAACTTTATCGAAAGACGAACAATCTGTCATCtaataca                                                                         >  1:7668495/1‑91 (MQ=255)

                tcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATaaa                                                                         >  2:2426130/1‑93 (MQ=255)

                tcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATaaa                                                                         <  2:222678/93‑1 (MQ=255)

                tcCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATATGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATaaa                                                                         >  2:3308165/1‑93 (MQ=255)

                  ccGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATACACg                                                                       >  1:3388050/1‑93 (MQ=255)

                    gAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACgga                                                                     >  2:6317354/1‑92 (MQ=255)

                      gCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTaa                                                                   >  1:1869425/1‑93 (MQ=255)

                      gCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTaa                                                                   >  2:1351914/1‑93 (MQ=255)

                      gCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTaa                                                                   <  2:1484524/93‑1 (MQ=255)

                       cAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTaaa                                                                  <  1:3165329/93‑1 (MQ=255)

                       cAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTaaa                                                                  <  1:611597/93‑1 (MQ=255)

                         aaaGAAATCTAAGTTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACa                                                                <  1:4816910/93‑1 (MQ=255)

                          aaGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACAt                                                               >  2:3924375/1‑93 (MQ=255)

                           aGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACAtt                                                              >  2:556421/1‑93 (MQ=255)

                           aGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACAtt                                                              <  2:2828455/93‑1 (MQ=255)

                           aGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACAtt                                                              >  2:4961237/1‑93 (MQ=255)

                            gAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTc                                                             <  1:7211668/93‑1 (MQ=255)

                            gAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTc                                                             <  2:6026038/93‑1 (MQ=255)

                              aaTCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCtt                                                           <  1:468802/93‑1 (MQ=255)

                              aaTCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCtt                                                           <  2:477823/93‑1 (MQ=255)

                              aaTCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCtt                                                           <  1:2814790/93‑1 (MQ=255)

                                 cTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCttttt                                                        >  2:2500513/1‑93 (MQ=255)

                                 cTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCttttt                                                        >  1:4627679/1‑93 (MQ=255)

                                 cTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCttttt                                                        <  1:6642651/93‑1 (MQ=255)

                                  tAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTta                                                       >  1:6242478/1‑93 (MQ=255)

                                  tAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTta                                                       <  1:1799524/93‑1 (MQ=255)

                                   aaGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTtat                                                      <  1:5326854/93‑1 (MQ=255)

                                   aaGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCAAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTtaa                                                      <  2:263415/93‑2 (MQ=255)

                                    aGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTtata                                                     <  1:350920/93‑1 (MQ=255)

                                    aGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTtata                                                     <  2:6094925/93‑1 (MQ=255)

                                     gCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTatat                                                    >  1:6737715/1‑93 (MQ=255)

                                     gCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTatat                                                    >  1:5770560/1‑93 (MQ=255)

                                     gCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTatat                                                    <  1:3064167/93‑1 (MQ=255)

                                       tgtgTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTc                                                  <  2:649222/93‑1 (MQ=255)

                                       tgtgTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTc                                                  >  2:3956707/1‑93 (MQ=255)

                                        gtgtAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCa                                                 <  2:7669589/93‑1 (MQ=255)

                                        gtgtAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCa                                                 <  1:7039070/93‑1 (MQ=255)

                                        gtgtAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCa                                                 <  2:2965049/93‑1 (MQ=255)

                                        gtgtAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCa                                                 <  1:4048329/93‑1 (MQ=255)

                                         tgtAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTAATCTAATAAACGGTAAACATTCTTTTTATATTCAc                                                <  1:2602083/93‑1 (MQ=255)

                                           tAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACgg                                              <  1:2978812/93‑1 (MQ=255)

                                           tAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACgg                                              <  2:4068263/93‑1 (MQ=255)

                                            aaCAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGc                                             <  2:3994252/93‑1 (MQ=255)

                                             aCAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAAAATTCTTTTTATATTCACGGCa                                            <  1:1413777/93‑1 (MQ=255)

                                               aaGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCAtt                                          >  2:1630035/1‑93 (MQ=255)

                                               aaGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCAtt                                          >  2:4191628/1‑93 (MQ=255)

                                               aaGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCAtt                                          >  2:5852683/1‑93 (MQ=255)

                                                aGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTa                                         <  2:1175498/93‑1 (MQ=255)

                                                aGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTa                                         >  2:7542678/1‑93 (MQ=255)

                                                 gTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAACCGGTAAACATTCTTTTTATATTCACGGCATTAc                                        <  1:954356/93‑1 (MQ=255)

                                                 gTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTAc                                        >  1:1467445/1‑93 (MQ=255)

                                                 gTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTAc                                        <  1:4475698/93‑1 (MQ=255)

                                                  tAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACt                                       <  2:3738459/93‑1 (MQ=255)

                                                    aaCGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGa                                     <  1:1940124/93‑1 (MQ=255)

                                                    aaCGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGa                                     <  2:3563628/93‑1 (MQ=255)

                                                    aaCGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGa                                     <  2:1908622/93‑1 (MQ=255)

                                                    aaCGACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGa                                     <  1:68098/93‑1 (MQ=255)

                                                       gACTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATaa                                  <  2:5462228/93‑1 (MQ=255)

                                                        aCTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATaaa                                 <  2:3184002/93‑1 (MQ=255)

                                                        aCTAATTTGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATaaa                                 >  1:5895448/1‑93 (MQ=255)

                                                             tttGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGt                            <  1:3857790/93‑1 (MQ=255)

                                                              ttGACCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTc                           <  1:1082176/93‑1 (MQ=255)

                                                                 aCCGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGct                        >  2:52037/1‑93 (MQ=255)

                                                                  ccGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGctc                       <  2:3590247/93‑1 (MQ=255)

                                                                  ccGGAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGctc                       >  2:2366345/1‑93 (MQ=255)

                                                                    ggAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCtctc                     <  1:6967285/93‑1 (MQ=255)

                                                                     gAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCg                    >  1:5475853/1‑93 (MQ=255)

                                                                     gAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCg                    >  2:4997216/1‑93 (MQ=255)

                                                                     gAAACTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCg                    >  1:2875704/1‑93 (MQ=255)

                                                                       aaCTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCa                  >  1:811911/1‑93 (MQ=255)

                                                                        aCTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTATTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCAt                 <  2:4583972/93‑1 (MQ=255)

                                                                         cTTTAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATa                >  2:6392233/1‑93 (MQ=255)

                                                                          tttAGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATaa               <  1:4138336/93‑1 (MQ=255)

                                                                             aGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAAt            >  2:202442/1‑93 (MQ=255)

                                                                             aGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAAt            >  1:7806908/1‑93 (MQ=255)

                                                                             aGCGAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAAt            <  2:6885191/93‑1 (MQ=255)

                                                                                gAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTa         >  2:533080/1‑93 (MQ=255)

                                                                                gAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTa         >  2:5097500/1‑93 (MQ=255)

                                                                                gAAAGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTa         >  1:3590692/1‑93 (MQ=255)

                                                                                  aaGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTaca       <  2:323592/93‑1 (MQ=255)

                                                                                  aaGACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTaca       >  1:4975632/1‑93 (MQ=255)

                                                                                    gACGCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTACACt     >  1:5116453/1‑93 (MQ=255)

                                                                                       gCACAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTACACTTGc  <  1:171662/93‑1 (MQ=255)

GATCCTGACATGCCTCTCCCGAGCAAAAGAAATCTAAGCTGTGTAACAAGTAAACGACTAATTTGACCGGAAACTTTAGCGAAAGACGCATAATCTGTCATCTAATAAACGGTAAACATTCTTTTTATATTCACGGCATTACTGATAAAAAAGTCGCTCTCGCATAAAATTTACACTTGC  >  NC_000913/2947022‑2947201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`