BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,854	T→C	L271P (CTG→CCG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,854	0	T	C	99.7%	884.3 / NA	287	L271P (CTG→CCG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (131/155); minor base A (1/0); total (132/155)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

cGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGa                                                                                         >  1:6064222/1‑93 (MQ=255)

cGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGa                                                                                         <  1:6155067/93‑1 (MQ=255)

 ggtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGAt                                                                                        <  2:1865690/93‑1 (MQ=255)

  gtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATc                                                                                       >  2:7412666/1‑93 (MQ=255)

  gtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATc                                                                                       >  1:4157371/1‑93 (MQ=255)

   tgtttAACCCGCTCACCGCACTAGTTTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATcc                                                                                      <  2:6309672/90‑1 (MQ=255)

      ttAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCCATCCGcc                                                                                   >  1:7175576/1‑93 (MQ=255)

        aaCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGcccc                                                                                 <  1:3856525/93‑1 (MQ=255)

          cccGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCt                                                                               >  2:1498886/1‑93 (MQ=255)

          cccGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCt                                                                               <  2:5014345/93‑1 (MQ=255)

            cGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGc                                                                             >  1:1782460/1‑93 (MQ=255)

            cGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGc                                                                             >  2:3436751/1‑93 (MQ=255)

              cTCACCGCACTAGATTATGCGACAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAc                                                                           <  2:4773817/93‑1 (MQ=255)

               tCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAcc                                                                          >  1:5076003/1‑93 (MQ=255)

               tCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAcc                                                                          >  2:3256546/1‑93 (MQ=255)

                 aCCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGc                                                                        >  2:5895655/1‑93 (MQ=255)

                 aCCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGc                                                                        >  1:6862012/1‑93 (MQ=255)

                   cGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGccc                                                                      <  1:7507524/93‑1 (MQ=255)

                     cACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGt                                                                    >  2:7097946/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCCGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  1:3966236/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  2:674033/1‑93 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtca                                                                  >  1:5246769/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  1:328262/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  2:3749903/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  1:3631969/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 <  1:3650876/93‑1 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  2:6558467/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGGTCCGTTCACCGTCAGCCCGATCCGCACCCTGCACCGCCCGgcat                                                                 >  2:7744308/1‑93 (MQ=255)

                         aGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatc                                                                <  1:5829801/93‑1 (MQ=255)

                          gATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatca                                                               >  1:547118/1‑93 (MQ=255)

                          aaTTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatca                                                               <  1:2121524/92‑1 (MQ=255)

                            ttATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgc                                                             <  2:1071712/93‑1 (MQ=255)

                            ttATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgc                                                             >  2:1608314/1‑93 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           <  2:7931454/93‑1 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           >  1:4375152/1‑93 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCACCAgcgc                                                           >  2:2715797/1‑93 (MQ=255)

                               tGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCt                                                          >  2:3611898/1‑93 (MQ=255)

                               tGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCt                                                          <  1:7551583/93‑1 (MQ=255)

                                gCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTg                                                         <  2:2383549/93‑1 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  1:2976231/93‑1 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  2:169347/93‑1 (MQ=255)

                                 cGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTgg                                                        <  2:5746654/93‑1 (MQ=255)

                                   gCAAGCGGTCTAGTGGTGCGGCGATTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGtt                                                      <  1:5107794/93‑1 (MQ=255)

                                    cAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTc                                                     <  1:3051361/93‑1 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    <  1:6645665/93‑1 (MQ=255)

                                      aGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAg                                                   <  1:7027212/93‑1 (MQ=255)

                                       gCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAgg                                                  <  2:2324362/93‑1 (MQ=255)

                                        cGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGc                                                 <  1:7698933/93‑1 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               >  2:4503128/1‑93 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               >  2:5987708/1‑93 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCAGCCCCCTGCACCGCCCGTCATCCGCGCTGGTTCAGGCGt                                               >  2:5316645/1‑93 (MQ=255)

                                           tttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGtt                                              >  1:776090/1‑93 (MQ=255)

                                           tttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGtt                                              >  2:2006807/1‑93 (MQ=255)

                                            ttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGCTCAGGCGgtt                                             >  2:2139252/1‑93 (MQ=255)

                                             tAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTa                                            <  1:736257/93‑1 (MQ=255)

                                             tAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTa                                            <  1:1721371/93‑1 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAg                                           <  1:2132452/93‑1 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAg                                           <  1:4274843/93‑1 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          >  2:4154127/1‑93 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          <  1:4522577/93‑1 (MQ=255)

                                                     cggcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAt                                    <  2:7247021/93‑1 (MQ=255)

                                                      ggcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAtt                                   >  1:7833321/1‑93 (MQ=255)

                                                       gcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAttt                                  >  1:5549129/1‑93 (MQ=255)

                                                         ggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTAc                                >  1:1346686/1‑93 (MQ=255)

                                                          gTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACa                               >  2:180749/1‑93 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTTGTTCAGGCGTTTAGTGGGCATTTACAAg                             <  1:1442689/93‑1 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             >  2:7343884/1‑93 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             <  1:3499395/93‑1 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             <  1:6568134/93‑1 (MQ=255)

                                                              aGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCg                           >  2:6288809/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          >  1:626001/1‑93 (MQ=255)

                                                               gTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgg                          <  1:4666904/93‑1 (MQ=255)

                                                                gaTTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         <  1:1879616/92‑1 (MQ=255)

                                                                  ttGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGt                       <  1:1530237/93‑1 (MQ=255)

                                                                  ttGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGt                       <  2:2462104/93‑1 (MQ=255)

                                                                  ttGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGt                       <  2:5459467/93‑1 (MQ=255)

                                                                    gCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTa                     <  2:2879683/93‑1 (MQ=255)

                                                                    gCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTa                     <  2:277851/93‑1 (MQ=255)

                                                                       gTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCg                  >  1:227820/1‑93 (MQ=255)

                                                                       gTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCg                  <  1:3196285/93‑1 (MQ=255)

                                                                          ccGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaaa               >  1:6310303/1‑93 (MQ=255)

                                                                          ccGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGaaa               >  2:370781/1‑93 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              <  1:2535829/93‑1 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              >  2:4027164/1‑93 (MQ=255)

                                                                           cGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAAc              >  2:706098/1‑93 (MQ=255)

                                                                            gTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACt             >  2:5553408/1‑93 (MQ=255)

                                                                            gTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACt             >  2:6962441/1‑93 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTg            <  2:175845/93‑1 (MQ=255)

                                                                              tCACCGTCAGCCCGATCCGCCCCGTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTgg           <  2:4294525/93‑1 (MQ=255)

                                                                              tCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTgg           <  2:3808189/93‑1 (MQ=255)

                                                                               cACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGt          <  2:5669060/93‑1 (MQ=255)

                                                                                aCCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTc         >  2:7520627/1‑93 (MQ=255)

                                                                                aCCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTc         <  2:4666365/93‑1 (MQ=255)

                                                                                aCCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTc         >  2:5157424/1‑93 (MQ=255)

                                                                                 ccGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCa        <  2:2939772/93‑1 (MQ=255)

                                                                                    tCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACtt     >  1:5756978/1‑93 (MQ=255)

                                                                                    tCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACtt     >  2:4216912/1‑93 (MQ=255)

                                                                                    tCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACtt     >  1:116613/1‑93 (MQ=255)

                                                                                      aGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtct   <  1:4878240/93‑1 (MQ=255)

                                                                                       gCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTtctc  <  1:124811/93‑1 (MQ=255)

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`